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          • Primary Antibodies ›
          • U2AF1 Antibodies

          Proteintech

          U2AF35 Monoclonal Antibody (2B7F11)

          4 References
          View all (16) U2AF1 antibodies

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          Datasheet
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          Cite U2AF35 Monoclonal Antibody (2B7F11)

          • Antibody Testing Data (9)
          U2AF35 Antibody in Immunocytochemistry (ICC/IF)
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          U2AF35 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          U2AF35 Antibody (60289-1-IG) in ICC/IF

          Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 60289-1-IG (U2AF35 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          U2AF35 Antibody in Immunocytochemistry (ICC/IF)
          U2AF35 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          U2AF35 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          U2AF35 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          U2AF35 Antibody in Western Blot (WB)
          U2AF35 Antibody in Western Blot (WB)
          U2AF35 Antibody in Western Blot (WB)
          U2AF35 Antibody in Western Blot (WB)
          U2AF35 Antibody in Immunoprecipitation (IP)

          Product Details

          60289-1-IG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 4 publications 4 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          -

          Immunoprecipitation (IP)

          0.5-4.0 µg
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human

          Host/Isotype

          Mouse / IgG2c

          Class

          Monoclonal

          Type

          Antibody

          Clone

          2B7F11

          Immunogen

          U2AF35 Fusion Protein Ag0399 (26-240 aa encoded by BC001923)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • CoraLite 594
          • CoraLite Plus 488
          • CoraLite Plus 647
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1.11 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: ACRHGDRCS RLHNKPTFSQ TIALLNIYRN PQNSSQSADG LRCAVSDVEM QEHYDEFFEE VFTEMEEKYG EVEEMNVCDN LGDHLVGNVY VKFRREEDAE KAVIDLNNRW FNGQPIHAEL SPVTDFREAC CRQYEMGECT RGGFCNFMHL KPISRELRRE LYGRRRKKHR SRSRSRERRS RSRDRGRGGG GGGGGGGGGR ERDRRRSRDR ERSGRF (26-240 aa encoded by BC001923)

          Target Information

          This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Splicing factor U2AF 35 kDa subunit; splicing factor U2AF 35kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear ribonucleoprotein auxiliary factor (U2AF),35 kDa; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit; U2 small nuclear RNA auxiliary factor 1; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein

          View more View less

          Gene Aliases: 2010107D16Rik; 35kDa; FP793; RN; RNU2AF1; U2AF1; U2AF35; U2AFBP

          View more View less

          UniProt ID: (Human) Q01081, (Mouse) Q9D883

          View more View less

          Entrez Gene ID: (Human) 7307, (Mouse) 108121, (Rat) 687575

          View more View less

          Function(s)
          nucleotide binding protein binding poly(A) RNA binding metal ion binding nucleic acid binding RNA binding RS domain binding
          Process(es)
          mRNA processing RNA splicing mRNA splicing, via spliceosome termination of RNA polymerase II transcription RNA export from nucleus mRNA export from nucleus mRNA 3'-end processing regulation of mitophagy positive regulation of protein targeting to mitochondrion
          It has to be done as per old AB suggested Products section.
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