Proteintech

UBA5 Polyclonal Antibody

UBA5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

UBA5 Antibody (12093-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using UBA5 antibody 12093-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

UBA5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

UBA5 Antibody in Immunoprecipitation (IP)

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Product Details

12093-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

UBA5 Fusion Protein Ag2734 (105-404 aa encoded by BC009737)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: YDKVELANM NRLFFQPHQA GLSKVQAAEH TLRNINPDVL FEVHNYNITT VENFQHFMDR ISNGGLEEGK PVDLVLSCVD NFEARMTINT ACNELGQTWM ESGVSENAVS GHIQLIIPGE SACFACAPPL VVAANIDEKT LKREGVCAAS LPTTMGVVAG ILVQNVLKFL LNFGTVSFYL GYNAMQDFFP TMSMKPNPQC DDRNCRKQQE EYKKKVAALP KQEVIQEEEE IIHEDNEWGI ELVSEVSEEE LKNFSGPVPD LPEGITVAYT IPKKQEDSVT ELTVEDSGES LEDLMAKMKN M (105-404 aa encoded by BC009737)

Target Information

This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis.

Journal of cell science

Eck F,Phuyal S,Smith MD,Kaulich M,Wilkinson S,Farhan H,Behrends C

Wed Sep 16 00:00:00 UTC 2020

Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy.

American journal of human genetics

Colin E,Daniel J,Ziegler A,Wakim J,Scrivo A,Haack TB,Khiati S,Denommé AS,Amati-Bonneau P,Charif M,Procaccio V,Reynier P,Aleck KA,Botto LD,Herper CL,Kaiser CS,Nabbout R,N'Guyen S,Mora-Lorca JA,Assmann B,Christ S,Meitinger T,Strom TM,Prokisch H,Miranda-Vizuete A,Hoffmann GF,Lenaers G,Bomont P,Liebau E,Bonneau D

Thu Sep 01 00:00:00 UTC 2016

Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy.

American journal of human genetics

Thu Sep 01 00:00:00 UTC 2016

Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway.

The Journal of biological chemistry

Gavin JM,Hoar K,Xu Q,Ma J,Lin Y,Chen J,Chen W,Bruzzese FJ,Harrison S,Mallender WD,Bump NJ,Sintchak MD,Bence NF,Li P,Dick LR,Gould AE,Chen JJ

Fri Aug 15 00:00:00 UTC 2014

Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway.

The Journal of biological chemistry

Gavin JM,Hoar K,Xu Q,Ma J,Lin Y,Chen J,Chen W,Bruzzese FJ,Harrison S,Mallender WD,Bump NJ,Sintchak MD,Bence NF,Li P,Dick LR,Gould AE,Chen JJ

Fri Aug 15 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: FLJ23251; FLJ23251UBA5; ThiFP1; UBA5, ubiquitin-activating enzyme E1 homolog; Ubiquitin-activating enzyme 5; Ubiquitin-activating enzyme E1 domain-containing protein 1; ubiquitin-activating enzyme E1-domain containing 1; ubiquitin-like modifier activating enzyme 5; Ubiquitin-like modifier-activating enzyme 5; UFM1-activating enzyme

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Gene Aliases: 5730525G14Rik; AW240750; THIFP1; UBA5; UBE1DC1

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UniProt ID: (Human) Q9GZZ9, (Mouse) Q8VE47

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Entrez Gene ID: (Human) 79876, (Mouse) 66663

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Function(s)

protein binding ATP binding metal ion binding tRNA threonylcarbamoyladenosine dehydratase UFM1 activating enzyme activity nucleotide binding small protein activating enzyme activity

Process(es)

regulation of intracellular estrogen receptor signaling pathway response to endoplasmic reticulum stress protein ufmylation protein K69-linked ufmylation cyclic threonylcarbamoyladenosine biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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UBA5 Polyclonal Antibody

UBA5 Antibody (12093-1-AP) in ICC/IF

Product Details

12093-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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