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Rockland
UBC12 Polyclonal Antibody
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FIGURE: 1 / 2
UBC12 Antibody (600-401-865) in WB
Western blot analysis is shown using Rocklands Affinity Purified anti-UBC12 antibody to detect Human UBC12 in various preparations. This western blot shows reactivity with purified human UBC12 protein (lane 1), NIH-3T3 cells over expressing UBC12 by infection (lane 2) and endogenous UBC12 in NIH 3T3 cells (lane 3). Peptide competition (lane 4) blocks specific reactivity of the antibody with purified UBC12 protein. Comparison to a molecular weight marker (not shown) indicates a single band of ~21.0 kDa corresponding to the expected molecula... View More
Product Details
600-401-865
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chimpanzee,
Human,
Mouse,
Rat,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region near aa 150-183 of Human UBC12 protein.
Conjugate
Form
Liquid
Concentration
0.73 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Reactivity occurs against human UBC12 protein. However, 100% homology is on record for this protein from human, chimpanzee, frog, mouse, rat and dog as indicated by BLAST analysis. Reactivity with UBC12 proteins from other sources is not known.
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NEDD8 carrier protein; NEDD8 protein ligase; NEDD8-conjugating enzyme Ubc12; UBC12 homolog, yeast; ubiquitin C, related sequence 2; ubiquitin carrier protein M; ubiquitin conjugating enzyme E2M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog
Gene Aliases: 2510040H03Rik; hUbc12; UBC-RS2; UBC12; UBE2M
UniProt ID: (Human) P61081, (Mouse) P61082
Entrez Gene ID: (Human) 9040, (Dog) 484221, (Mouse) 22192, (Rat) 361509
ubiquitin-protein transferase activity
protein binding
ATP binding
ribosomal S6-glutamic acid ligase activity
NEDD8 transferase activity
ubiquitin protein ligase binding
ubiquitin protein ligase activity
nucleotide binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
molecular_function
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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