Invitrogen

UBC13 Monoclonal Antibody (4E 11)

FIGURE: 1 / 23

UBC13 Antibody (37-1100) in WB

Western blot analysis of HepG2 cell lysates using (A) Mms2 Monoclonal Antibody, Mouse (Product # 37-1100) and (B) Ubc13 Monoclonal Antibody, Mouse (Product # 37-1100).

Product Image Gallery

UBC13 Antibody — Figure 4 NSC697923 specifically inhibits Ubc13-mediated polyubiquitin chain synthesis in vitro. (A) NSC697923 inhibits the synthesis of polyubiquitin chains catalyzed by Ubc13-Uev1A in vitro. The in vitro reaction, which contains purified E1, Ub, Ubc13, and Uev1A, was carried out with or without the indicated concentrations of NSC697923. The reaction products were analyzed by an anti-Ub Ab on Western blots. The asterisk (*) denotes the signal from the cross-reaction of the Ab with the recombinant Ubc13 protein. (B) Same as in panel A, except that purified GST-TRAF6 was included as the E3 in the complete reaction as indicated. (C) NSC697923 exhibits no inhibitory effect on UbcH5c-mediated polyubiquitin chain synthesis. The assay was carried out as in panel B, except that UbcH5c instead of Ubc13-Uev1A was used as the E2 in the ubiquitination reaction. (D) NSC697923 has no inhibitory effect on the formation of Ubc13-Uev1A complex in vitro. Purified GST or GST-Ubc13 protein was incubated with the cell extracts prepared from OCI-Ly10 cells with or without the presence of NSC697923 as indicated. The amounts of Uev1A protein associated with GST or GST-Ubc13 were analyzed by Western blotting. The first lane was loaded with 8% of the input cell lysate. (E) NSC697923 inhibits the formation of the Ubc13~Ub thioester conjugate. The assay was carried out as described in panel A, except that the loading buffer for SDS-PAGE gel contained no reducing agents unless otherwise specified and the {TM}

Product Details

37-1100

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 29 publications 29 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bacteria, Human, Mouse, Plant

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

4E 11

Immunogen

Recombinant human Ubc13

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533298

Target Information

The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIG-I/DDX58 to activate the downstream signaling pathway that leads to interferon beta production. UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway. (Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The lncRNA 'UCA1' modulates the response to chemotherapy of ovarian cancer through direct binding to miR-27a-5p and control of UBE2N levels.

Molecular oncology

Wambecke A,Ahmad M,Morice PM,Lambert B,Weiswald LB,Vernon M,Vigneron N,Abeilard E,Brotin E,Figeac M,Gauduchon P,Poulain L,Denoyelle C,Meryet-Figuiere M

37-1100 was used in Western Blotting to demonstrate that downregulation of the short isoform of UCA1 sensitized OC cells to cisplatin and that UCA1 acted as competing endogenous RNA to miR-27a-5p.

Wed Dec 01 00:00:00 UTC 2021

The lncRNA 'UCA1' modulates the response to chemotherapy of ovarian cancer through direct binding to miR-27a-5p and control of UBE2N levels.

Molecular oncology

Wambecke A,Ahmad M,Morice PM,Lambert B,Weiswald LB,Vernon M,Vigneron N,Abeilard E,Brotin E,Figeac M,Gauduchon P,Poulain L,Denoyelle C,Meryet-Figuiere M

37-1100 was used in Western Blotting to demonstrate that downregulation of the short isoform of UCA1 sensitized OC cells to cisplatin and that UCA1 acted as competing endogenous RNA to miR-27a-5p.

Wed Dec 01 00:00:00 UTC 2021

Ubc13 Promotes K63-Linked Polyubiquitination of NLRP3 to Activate Inflammasome.

Journal of immunology (Baltimore, Md. : 1950)

Ni J,Guan C,Liu H,Huang X,Yue J,Xiang H,Jiang Z,Tao Y,Cao W,Liu J,Wang Z,Wang Y,Wu X

37-1100 was used in Western Blotting to shed light on mechanisms of NLRP3 inflammasome activation and identifies that targeting Ubc13 could be an effective therapeutic strategy for treating aberrant NLRP3 inflammasome activation-induced pathogenesis.

Sat May 15 00:00:00 UTC 2021

Abraxas suppresses DNA end resection and limits break-induced replication by controlling SLX4/MUS81 chromatin loading in response to TOP1 inhibitor-induced DNA damage.

Nature communications

Wu X,Wang B

37-1100 was used in Western Blot to implicate Abraxas/BRCA1-A complex as a critical regulator that restrains BIR for protection of genome stability.

Fri Jul 16 00:00:00 UTC 2021

Abraxas suppresses DNA end resection and limits break-induced replication by controlling SLX4/MUS81 chromatin loading in response to TOP1 inhibitor-induced DNA damage.

Nature communications

Wu X,Wang B

37-1100 was used in Western Blot to implicate Abraxas/BRCA1-A complex as a critical regulator that restrains BIR for protection of genome stability.

Fri Jul 16 00:00:00 UTC 2021

Immune regulator LGP2 targets Ubc13/UBE2N to mediate widespread interference with K63 polyubiquitination and NF-κB activation.

Cell reports

Lenoir JJ,Parisien JP,Horvath CM

37-1100 was used in Immunoprecipitation to establish a unifying mechanism for LGP2-mediated negative regulation that can modulate a variety of K63-Ub signaling pathways.

Tue Dec 28 00:00:00 UTC 2021

Legionella pneumophila regulates the activity of UBE2N by deamidase-mediated deubiquitination.

The EMBO journal

Gan N,Guan H,Huang Y,Yu T,Fu J,Nakayasu ES,Puvar K,Das C,Wang D,Ouyang S,Luo ZQ

37-1100 was used in Western Blotting to establish a deubiquitination mechanism catalyzed by a deamidase, which, together with MavC, imposes temporal regulation of the activity of UBE2N during L. pneumophila infection.

Mon Feb 17 00:00:00 UTC 2020

Advanced Cataloging of Lysine-63 Polyubiquitin Networks by Genomic, Interactome, and Sensor-Based Proteomic Analyses.

The Plant cell

Romero-Barrios N,Monachello D,Dolde U,Wong A,San Clemente H,Cayrel A,Johnson A,Lurin C,Vert G

37-1100 was used in Western Blotting to strongly increase our understanding of K63 polyubiquitination networks and functions in plants.

Wed Jan 01 00:00:00 UTC 2020

Legionella effector MavC targets the Ube2N~Ub conjugate for noncanonical ubiquitination.

Nature communications

Puvar K,Iyer S,Fu J,Kenny S,Negrón Terón KI,Luo ZQ,Brzovic PS,Klevit RE,Das C

37-1100 was used in Western Blotting to demonstrate that Ubiquitin exhibits weak binding to MavC alone, but shows an increase in affinity when tethered to Ube2N in a disulfide-linked substrate that mimics the charged E2~Ub conjugate.

Tue May 12 00:00:00 UTC 2020

Legionella effector MavC targets the Ube2N~Ub conjugate for noncanonical ubiquitination.

Nature communications

Puvar K,Iyer S,Fu J,Kenny S,Negrón Terón KI,Luo ZQ,Brzovic PS,Klevit RE,Das C

Tue May 12 00:00:00 UTC 2020

Legionella pneumophila inhibits immune signalling via MavC-mediated transglutaminase-induced ubiquitination of UBE2N.

Nature microbiology

Gan N,Nakayasu ES,Hollenbeck PJ,Luo ZQ

37-1100 was used in Western Blotting to identify a mechanism by which Legionella pneumophila modifies a key ubiquitination enzyme by ubiquitin transglutamination in order to modulate host immunity.

Tue Jan 01 00:00:00 UTC 2019

ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability.

Molecular cell

Haahr P,Borgermann N,Guo X,Typas D,Achuthankutty D,Hoffmann S,Shearer R,Sixma TK,Mailand N

37-1100 was used in Western Blotting to study the role of zinc finger protein UFM1 specific peptidase domain protein on genome stability.

Thu Apr 05 00:00:00 UTC 2018

Genetic rescue of lineage-balanced blood cell production reveals a crucial role for STAT3 antiinflammatory activity in hematopoiesis.

Proceedings of the National Academy of Sciences of the United States of America

Zhang H,Li HS,Hillmer EJ,Zhao Y,Chrisikos TT,Hu H,Wu X,Thompson EJ,Clise-Dwyer K,Millerchip KA,Wei Y,Puebla-Osorio N,Kaushik S,Santos MA,Wang B,Garcia-Manero G,Wang J,Sun SC,Watowich SS

37-1100 was used in Western Blotting to elucidate a crucial role for STAT3 anti-inflammatory activity in the preservation of haematopoietic stem and progenitor cells and lineage-balanced haematopoiesis.

Tue Mar 06 00:00:00 UTC 2018

OTUB1 non-catalytically stabilizes the E2 ubiquitin-conjugating enzyme UBE2E1 by preventing its autoubiquitination.

The Journal of biological chemistry

Pasupala N,Morrow ME,Que LT,Malynn BA,Ma A,Wolberger C

37-1100 was used in Western Blotting to elucidate a novel role for OTUB1-E2 interactions in modulating E2 protein ubiquitination.

Fri Nov 23 00:00:00 UTC 2018

Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity.

Molecular cell

Vujanovic M,Krietsch J,Raso MC,Terraneo N,Zellweger R,Schmid JA,Taglialatela A,Huang JW,Holland CL,Zwicky K,Herrador R,Jacobs H,Cortez D,Ciccia A,Penengo L,Lopes M

37-1100 was used in Western Blotting to show that damage-induced fork reversal in mammalian cells requires PCNA ubiquitination, UBC13, and K63-linked polyubiquitin chains.

Thu Sep 07 00:00:00 UTC 2017

HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains.

PLoS pathogens

Shibata Y,Tokunaga F,Goto E,Komatsu G,Gohda J,Saeki Y,Tanaka K,Takahashi H,Sawasaki T,Inoue S,Oshiumi H,Seya T,Nakano H,Tanaka Y,Iwai K,Inoue JI

371100 was used in western blot to discover that Tax recruits linear (Met1-linked) ubiquitin chain assembly complex to the IkB kinase complex

Sun Jan 01 00:00:00 UTC 2017

A High-Throughput Screening Strategy for Development of RNF8-Ubc13 Protein-Protein Interaction Inhibitors.

SLAS discovery : advancing life sciences R & D

Weber E,Rothenaigner I,Brandner S,Hadian K,Schorpp K

371100 was used in western blot to find inhibitors of the RNF8-Ubc13 interaction

Wed Mar 01 00:00:00 UTC 2017

Human T-cell leukemia virus type 1 (HTLV-1) tax requires CADM1/TSLC1 for inactivation of the NF-κB inhibitor A20 and constitutive NF-κB signaling.

PLoS pathogens

Pujari R,Hunte R,Thomas R,van der Weyden L,Rauch D,Ratner L,Nyborg JK,Ramos JC,Takai Y,Shembade N

37-1100 was used in Western Blotting to demonstrate that membrane associated Cell adhesion molecule1 recruits Ubc13 to Tax, causing K63-linked polyubiquitination of Tax, and IKK complex activation in the membrane lipid raft.

Sun Mar 01 00:00:00 UTC 2015

Covalent Inhibition of Ubc13 Affects Ubiquitin Signaling and Reveals Active Site Elements Important for Targeting.

ACS chemical biology

Hodge CD,Edwards RA,Markin CJ,McDonald D,Pulvino M,Huen MS,Zhao J,Spyracopoulos L,Hendzel MJ,Glover JN

37-1100 was used in Western Blotting to elucidate the structures of complexes of Ubc13 with two inhibitors, NSC697923 and BAY 11-7082, which inhibit DNA damage and NF-κB signalling in human cells.

Fri Jul 17 00:00:00 UTC 2015

USP21 negatively regulates antiviral response by acting as a RIG-I deubiquitinase.

The Journal of experimental medicine

Fan Y,Mao R,Yu Y,Liu S,Shi Z,Cheng J,Zhang H,An L,Zhao Y,Xu X,Chen Z,Kogiso M,Zhang D,Zhang H,Zhang P,Jung JU,Li X,Xu G,Yang J

37-1100 was used in western blot to report a role for USP21 in the negative regulation of antiviral response through deubiquitinating RIG-I

Mon Feb 10 00:00:00 UTC 2014

A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.

Journal of cell science

Fiesel FC,Moussaud-Lamodière EL,Ando M,Springer W

37-1100 was used in western blot to identify E2s that regulate the activation, translocation, and enzymatic functions of Parkin during mitochondrial quality control

Fri Aug 15 00:00:00 UTC 2014

STAT3 restrains RANK- and TLR4-mediated signalling by suppressing expression of the E2 ubiquitin-conjugating enzyme Ubc13.

Nature communications

Zhang H,Hu H,Greeley N,Jin J,Matthews AJ,Ohashi E,Caetano MS,Li HS,Wu X,Mandal PK,McMurray JS,Moghaddam SJ,Sun SC,Watowich SS

37-1100 was used in western blot to elucidate the mechanism by which STAT3 controls NF-kapaB signaling in innate immune cells.

Mon Dec 15 00:00:00 UTC 2014

A small-molecule inhibitor of UBE2N induces neuroblastoma cell death via activation of p53 and JNK pathways.

Cell death & disease

Cheng J,Fan YH,Xu X,Zhang H,Dou J,Tang Y,Zhong X,Rojas Y,Yu Y,Zhao Y,Vasudevan SA,Zhang H,Nuchtern JG,Kim ES,Chen X,Lu F,Yang J

37-1100 was used in western blot to study the effect of a UBE2N inhibitor on neuroblastoma and its mechanism.

Thu Feb 20 00:00:00 UTC 2014

E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1.

Nature structural & molecular biology

Wiener R,DiBello AT,Lombardi PM,Guzzo CM,Zhang X,Matunis MJ,Wolberger C

37-1100 was used in western blot to assess the deubiquitinating activity of OTUB1 regulated by E2 ubiquitin-conjugating enzymes

Sun Sep 01 00:00:00 UTC 2013

Inhibition of proliferation and survival of diffuse large B-cell lymphoma cells by a small-molecule inhibitor of the ubiquitin-conjugating enzyme Ubc13-Uev1A.

Blood

Pulvino M,Liang Y,Oleksyn D,DeRan M,Van Pelt E,Shapiro J,Sanz I,Chen L,Zhao J

37-1100 was used in western blot to identify and characterize compounds that inhibit NF-κB activation in diffuse large B-cell lymphoma cells.

Thu Aug 23 00:00:00 UTC 2012

Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress.

Molecular cell

Ciccia A,Nimonkar AV,Hu Y,Hajdu I,Achar YJ,Izhar L,Petit SA,Adamson B,Yoon JC,Kowalczykowski SC,Livingston DM,Haracska L,Elledge SJ

37-1100 was used in western blot to study the role of ZRANB3 translocase during replication.

Fri Aug 10 00:00:00 UTC 2012

A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling.

Science (New York, N.Y.)

Cotta-Ramusino C,McDonald ER,Hurov K,Sowa ME,Harper JW,Elledge SJ

Published figure using UBC13 monoclonal antibody (Product # 37-1100) in Western Blot

Fri Jun 10 00:00:00 UTC 2011

The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage.

Cell

Stewart GS,Panier S,Townsend K,Al-Hakim AK,Kolas NK,Miller ES,Nakada S,Ylanko J,Olivarius S,Mendez M,Oldreive C,Wildenhain J,Tagliaferro A,Pelletier L,Taubenheim N,Durandy A,Byrd PJ,Stankovic T,Taylor AM,Durocher D

37-1100 was used in immunoprecipitation and western blot to show that RNF168 is mutated in individuals with RIDDLE syndrome

Fri Feb 06 00:00:00 UTC 2009

Regulation of hematopoiesis by the K63-specific ubiquitin-conjugating enzyme Ubc13.

Proceedings of the National Academy of Sciences of the United States of America

Wu X,Yamamoto M,Akira S,Sun SC

Tue Dec 08 00:00:00 UTC 2009

Noncanonical E2 variant-independent function of UBC13 in promoting checkpoint protein assembly.

Molecular and cellular biology

Huen MS,Huang J,Yuan J,Yamamoto M,Akira S,Ashley C,Xiao W,Chen J

Wed Oct 01 00:00:00 UTC 2008

Noncanonical E2 variant-independent function of UBC13 in promoting checkpoint protein assembly.

Molecular and cellular biology

Huen MS,Huang J,Yuan J,Yamamoto M,Akira S,Ashley C,Xiao W,Chen J

Wed Oct 01 00:00:00 UTC 2008

RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.

The Journal of biological chemistry

Sakasai R,Tibbetts R

37-1100 was used in western blot to investigate the potential role of the proteasome in checkpoint activation and ATM/ATR signaling in response to UV light-induced DNA damage

Fri May 16 00:00:00 UTC 2008

The human T-cell leukemia virus type 1 Tax oncoprotein requires the ubiquitin-conjugating enzyme Ubc13 for NF-kappaB activation.

Journal of virology

Shembade N,Harhaj NS,Yamamoto M,Akira S,Harhaj EW

37-1100 was used in immunoprecipitation and western blot to study the requirement of ubiquitin-conjugating enzyme Ubc13 for NF-kappaB activation by human T-cell leukemia virus 1 tax oncoprotein

Sat Dec 01 00:00:00 UTC 2007

Cutting Edge: Pivotal function of Ubc13 in thymocyte TCR signaling.

Journal of immunology (Baltimore, Md. : 1950)

Yamamoto M,Sato S,Saitoh T,Sakurai H,Uematsu S,Kawai T,Ishii KJ,Takeuchi O,Akira S

37-1100 was used in western blot to determine the function of Ubc13 in T cell receptor-mediated signaling and responses

Fri Dec 01 00:00:00 UTC 2006

Ubiquitination and translocation of TRAF2 is required for activation of JNK but not of p38 or NF-kappaB.

The EMBO journal

Habelhah H,Takahashi S,Cho SG,Kadoya T,Watanabe T,Ronai Z

Wed Jan 28 00:00:00 UTC 2004

TIFA activates IkappaB kinase (IKK) by promoting oligomerization and ubiquitination of TRAF6.

Proceedings of the National Academy of Sciences of the United States of America

Ea CK,Sun L,Inoue J,Chen ZJ

Tue Oct 26 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: bendless protein; bendless-like ubiquitin conjugating enzyme; Bendless-like ubiquitin-conjugating enzyme; E2 ubiquitin-conjugating enzyme N; epididymis secretory protein Li 71; MGC131857; MGC8489; Ubc13; UbcH13; Ubiquitin carrier protein N; ubiquitin conjugating enzyme E2N; Ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); Ubiquitin-protein ligase N; yeast UBC13 homolog

View more View less

Gene Aliases: 1500026J17Rik; AL022654; BB101821; BLU; HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN; UBC13; UBE2N

View more View less

UniProt ID: (Human) P61088, (Rat) Q9EQX9, (Mouse) P61089

View more View less

Entrez Gene ID: (Human) 7334, (Rat) 116725, (Mouse) 93765

View more View less

Function(s)

ubiquitin-protein transferase activity protein binding ATP binding ubiquitin protein ligase binding ubiquitin binding poly(A) RNA binding ubiquitin protein ligase activity ubiquitin conjugating enzyme activity ligase activity ubiquitin-like protein transferase activity nucleotide binding

Process(es)

double-strand break repair via homologous recombination DNA double-strand break processing postreplication repair ubiquitin-dependent protein catabolic process protein ubiquitination histone ubiquitination positive regulation of histone modification regulation of histone ubiquitination positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of DNA repair T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity positive regulation of ubiquitin-protein transferase activity protein K63-linked ubiquitination DNA repair cellular response to DNA damage stimulus stimulatory C-type lectin receptor signaling pathway regulation of DNA repair double-strand break repair via nonhomologous end joining cellular protein modification process proteolysis Fc-epsilon receptor signaling pathway nucleotide-binding oligomerization domain containing signaling pathway global genome nucleotide-excision repair

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

UBC13 Monoclonal Antibody (4E 11)

UBC13 Antibody (37-1100) in WB

Product Details

37-1100

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!