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Invitrogen
UBE2B Recombinant Rabbit Monoclonal Antibody (2Z3R2)
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FIGURE: 1 / 5
UBE2B Antibody (MA5-42600) in IHC (P)
Immunohistochemistry analysis of UBE2B in paraffin-embedded human esophageal cancer. Samples were incubated with UBE2B Monoclonal antibody (Product # MA5-42600) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
MA5-42600
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2Z3R2
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 53-152 of human UBE2B (P63146).
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911741
Product Specific Information
Positive test controls include: HeLa, 293T, NIH/3T3, K-562, Mouse testis, Mouse heart, Rat testis. The target is usually found in the following locations: Cell membrane, Nucleus.
Immunogen sequence: FKLVIEFSEE YPNKPPTVRF LSKMFHPNVY ADGSICLDIL QNRWSPTYDV SSILTSIQSL LDEPNPNSPA NSQAAQLYQE NKREYEKRVS AIVEQSWNDS
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2 protein; E2 ubiquitin-conjugating enzyme B; E2(14k); E214K; HR6B; RAD6 homolog B; Ubiquitin carrier protein B; ubiquitin conjugating enzyme; ubiquitin conjugating enzyme E2B; Ubiquitin-conjugating enzyme E2 B; Ubiquitin-conjugating enzyme E2(14k); Ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B (RAD6 homolog); ubiquitin-conjugating enzyme E2B (RAD6 homolog, S. cerevisiae); ubiquitin-conjugating enzyme E2B, RAD6 homolog; ubiquitin-conjugating enzyme E2B, RAD6 homology; Ubiquitin-protein ligase B
Gene Aliases: 2610301N02Rik; E2-14k; E2-17kDa; HHR6B; HR6B; mHR6B; RAD6B; UBC2; UBCH1; UBE2B
UniProt ID: (Human) P63146, (Rat) P63149, (Mouse) P63147
Entrez Gene ID: (Human) 7320, (Rat) 81816, (Mouse) 22210
protein polyubiquitination
response to hypoxia
in utero embryonic development
DNA repair
postreplication repair
maintenance of chromatin silencing
ubiquitin-dependent protein catabolic process
protein monoubiquitination
cellular response to DNA damage stimulus
spermatogenesis
sperm axoneme assembly
response to UV
positive regulation of reciprocal meiotic recombination
protein ubiquitination
histone ubiquitination
regulation of histone modification
cellular response to insulin stimulus
negative regulation of histone phosphorylation
histone H2A ubiquitination
response to drug
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of cAMP-mediated signaling
protein stabilization
chiasma assembly
protein autoubiquitination
canonical Wnt signaling pathway
histone lysine demethylation
synaptonemal complex organization
protein K63-linked ubiquitination
protein K48-linked ubiquitination
protein K11-linked ubiquitination
DNA damage response, detection of DNA damage
meiotic telomere clustering
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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