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UBE2D1 Antibody (PA5-102532) in ICC/IF
Immunocytochemistry analysis of UBE2D1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with UBE2D1 polyclonal antibody (Product # PA5-102532) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102532
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human UBE2D1(Accession P51668), corresponding to amino acid residues V102-M147.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2851934
Product Specific Information
Antibody detects endogenous levels of total UBE2D1.
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: (E3-independent) E2 ubiquitin-conjugating enzyme D1; E2 ubiquitin-conjugating enzyme D1; E2(17)KB 1; SFT; Stimulator of Fe transport; UBC 4/5; UBC4/5 homolog; UBCH 5; UBCH 5A; UbcH5; ubiquitin; Ubiquitin carrier protein D1; ubiquitin conjugating enzyme E2D 1; Ubiquitin-conjugating enzyme E2 D1; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2D 1, UBC4/5 homolog; Ubiquitin-protein ligase D1
Gene Aliases: E2(17)KB1; SFT; UBC4/5; UBC5A; UBCH5; UBCH5A; UBE2D1
UniProt ID: (Human) P51668, (Mouse) P61080, (Rat) D3ZDK2
Entrez Gene ID: (Human) 7321, (Mouse) 216080, (Rat) 361831
protein polyubiquitination
ubiquitin-dependent protein catabolic process
positive regulation of protein ubiquitination
protein K48-linked ubiquitination
negative regulation of transcription from RNA polymerase II promoter
stimulatory C-type lectin receptor signaling pathway
BMP signaling pathway
anaphase-promoting complex-dependent catabolic process
TRIF-dependent toll-like receptor signaling pathway
Fc-epsilon receptor signaling pathway
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
T cell receptor signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
regulation of transcription from RNA polymerase II promoter in response to hypoxia
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