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          • Primary Antibodies ›
          • UBE2E2 Antibodies

          Bethyl Laboratories

          UBE2E2 Polyclonal Antibody

          View all (8) UBE2E2 antibodies

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          Cite UBE2E2 Polyclonal Antibody

          • Antibody Testing Data (1)
          UBE2E2 Antibody in Western Blot (WB)
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          UBE2E2 Antibody in Western Blot (WB)
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          FIGURE: 1 / 1

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          UBE2E2 Antibody (A303-485A) in WB

          Detection of human UBE2E2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-UBE2E2 antibody (Product # A303-485A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 6 µg/mg lysate. UBE2E2 was also immunoprecipitated by rabbit anti-UBE2E2 antibody A303-484A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          UBE2E2 Antibody in Western Blot (WB)

          Product Details

          A303-485A

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          -

          Immunoprecipitation (IP)

          2-10 µg/mg lysate
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Region between residue 25 and 75 of Human Ubiquitin-conjugating Enzyme E2E 2.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.20 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          TBS, pH 7.0 to 8.0, with 0.1% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          The recommended shelf life for this product is 1 year from date of receipt.

          Target Information

          ubiquitin-conjugating enzyme E2 (UBE2E2) is a ubiquitin conjugating enzyme.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: E2 ubiquitin-conjugating enzyme E2; FLJ25157; UbcH8; Ubiquitin carrier protein E2; ubiquitin conjugating enzyme E2E 2; Ubiquitin-conjugating enzyme E2 E2; ubiquitin-conjugating enzyme E2E 2 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase E2

          View more View less

          Gene Aliases: UBCH8; UBE2E2

          View more View less

          UniProt ID: (Human) Q96LR5

          View more View less

          Entrez Gene ID: (Human) 7325

          View more View less

          Function(s)
          ubiquitin-protein transferase activity protein binding ATP binding ISG15 transferase activity ubiquitin conjugating enzyme activity
          Process(es)
          cellular response to DNA damage stimulus ISG15-protein conjugation protein K63-linked ubiquitination protein K48-linked ubiquitination protein K11-linked ubiquitination positive regulation of G1/S transition of mitotic cell cycle
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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