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UBE2J2 Antibody (600-401-B43) in WB
Western blot using Rocklands affinity purified anti-Ube2j2 antibody shows detection of Ube2j2 in 293 cells over-expressing Myc-Ube2j2 (Lane 1). Lane 2 contains lysate from mock-transfected 293 cells. Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD
Product Details
600-401-B43
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the human Ube2j2 protein.
Conjugate
Form
Liquid
Concentration
0.91 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Directed against human Ube2j2 protein. A BLAST analysis was used to suggest cross-reactivity with Ube2j2 protein from human, horse, opossum, cattle, dog, mouse, rat, macaque, salmon, chicken, zebra finch, Xenopus and platypus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2J2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2 ubiquitin-conjugating enzyme J2; NCUBE-2; Non-canonical ubiquitin-conjugating enzyme 2; Ubc6p homolog; ubiquitin conjugating enzyme 6; ubiquitin conjugating enzyme E2, J2; Ubiquitin-conjugating enzyme E2 J2; ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast); ubiquitin-conjugating enzyme E2, J2 homolog; yeast UBC6 homolog
Gene Aliases: 1200007B18Rik; 2400008G19Rik; 5730472G04Rik; AL022923; NCUBE-2; NCUBE2; PRO2121; Ubc6; Ubc6p; UBE2J2
UniProt ID: (Human) Q8N2K1, (Mouse) Q6P073
Entrez Gene ID: (Human) 118424, (Mouse) 140499, (Rat) 298689
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