Proteintech

UBE2L3 Polyclonal Antibody

View all (15) UBE2L3 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (10)

UBE2L3 Antibody in Western Blot (WB)

UBE2L3 Antibody in Western Blot (WB)

FIGURE: 1 / 10

UBE2L3 Antibody (14415-1-AP) in WB

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

UBE2L3 Antibody in Western Blot (WB)

UBE2L3 Antibody in Western Blot (WB)

UBE2L3 Antibody in Western Blot (WB)

UBE2L3 Antibody in Western Blot (WB)

UBE2L3 Antibody in Western Blot (WB)

UBE2L3 Antibody in Western Blot (WB)

UBE2L3 Antibody in Western Blot (WB)

UBE2L3 Antibody in Western Blot (WB)

UBE2L3 Antibody in Western Blot (WB)

UBE2L3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

UBE2L3 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

UBE2L3 Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

UBE2L3 Antibody in Western Blot (WB) — Rat kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

UBE2L3 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

UBE2L3 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

UBE2L3 Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

UBE2L3 Antibody in Western Blot (WB) — A431 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

UBE2L3 Antibody in Western Blot (WB) — MCF7 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

UBE2L3 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

UBE2L3 Antibody in Immunoprecipitation (IP) — IP result of anti-UBE2L3 (IP:14415-1-AP, 3ug; Detection:14415-1-AP 1:2000) with mouse kidney tissue lysate 4000ug.

Product Details

14415-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

UBE2L3 Fusion Protein Ag5758 (1-154 aa encoded by BC053368)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAASRRLMK ELEEIRKCGM KNFRNIQVDE ANLLTWQGLI VPDNPPYDKG AFRIEINFPA EYPFKPPKIT FKTKIYHPNI DEKGQVCLPV ISAENWKPAT KTDQVIQSLI ALVNDPQPEH PLRADLAEEY SKDRKKFCKN AEEFTKKYGE KRPVD (1-154 aa encoded by BC053368)

Target Information

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Cell communication and signaling : CCS

Xie X,Hu L,Mi B,Xue H,Hu Y,Panayi AC,Endo Y,Chen L,Yan C,Lin Z,Li H,Zhou W,Liu G

Tue Oct 25 00:00:00 UTC 2022

Metformin alleviates bone loss in ovariectomized mice through inhibition of autophagy of osteoclast precursors mediated by E2F1.

Cell communication and signaling : CCS

Xie X,Hu L,Mi B,Xue H,Hu Y,Panayi AC,Endo Y,Chen L,Yan C,Lin Z,Li H,Zhou W,Liu G

Tue Oct 25 00:00:00 UTC 2022

UBE2L3 promotes squamous cell carcinoma progression in the oral cavity and hypopharynx via activating the NF-κB signaling by increasing IκBα degradation.

Cell biology international

Cui Z,Sun S,Li J,Li J,Sha T,He J,Zuo L

Sun May 01 00:00:00 UTC 2022

UBE2L3 promotes squamous cell carcinoma progression in the oral cavity and hypopharynx via activating the NF-κB signaling by increasing IκBα degradation.

Cell biology international

Cui Z,Sun S,Li J,Li J,Sha T,He J,Zuo L

Sun May 01 00:00:00 UTC 2022

UBE2L3 promotes squamous cell carcinoma progression in the oral cavity and hypopharynx via activating the NF-κB signaling by increasing IκBα degradation.

Cell biology international

Cui Z,Sun S,Li J,Li J,Sha T,He J,Zuo L

Sun May 01 00:00:00 UTC 2022

UBE2L3 promotes squamous cell carcinoma progression in the oral cavity and hypopharynx via activating the NF-κB signaling by increasing IκBα degradation.

Cell biology international

Cui Z,Sun S,Li J,Li J,Sha T,He J,Zuo L

Sun May 01 00:00:00 UTC 2022

UBE2L3 promotes squamous cell carcinoma progression in the oral cavity and hypopharynx via activating the NF-κB signaling by increasing IκBα degradation.

Cell biology international

Cui Z,Sun S,Li J,Li J,Sha T,He J,Zuo L

Sun May 01 00:00:00 UTC 2022

UBE2L3 promotes squamous cell carcinoma progression in the oral cavity and hypopharynx via activating the NF-κB signaling by increasing IκBα degradation.

Cell biology international

Cui Z,Sun S,Li J,Li J,Sha T,He J,Zuo L

Sun May 01 00:00:00 UTC 2022

The Mechano-Ubiquitinome of Articular Cartilage: Differential Ubiquitination and Activation of a Group of ER-Associated DUBs and ER Stress Regulators.

Molecular & cellular proteomics : MCP

Kaokhum N,Pinto-Fernández A,Wilkinson M,Kessler BM,Ismail HM

Thu Dec 01 00:00:00 UTC 2022

UBC Mediated by SEPT6 Inhibited the Progression of Prostate Cancer.

Mediators of inflammation

Zhang R,Yang Y,Huang H,Li T,Ye L,Lin L,Wei Y

Thu Mar 31 00:00:00 UTC 2022

E2F transcription factor 1 (E2F1) promotes the transforming growth factor TGF-β1 induced human cardiac fibroblasts differentiation through promoting the transcription of CCNE2 gene.

Bioengineered

Liao R,Xie B,Cui J,Qi Z,Xue S,Wang Y

Wed Dec 01 00:00:00 UTC 2021

E2F transcription factor 1 (E2F1) promotes the transforming growth factor TGF-β1 induced human cardiac fibroblasts differentiation through promoting the transcription of CCNE2 gene.

Bioengineered

Liao R,Xie B,Cui J,Qi Z,Xue S,Wang Y

Wed Dec 01 00:00:00 UTC 2021

Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.

Frontiers in immunology

Ye X,Pan T,Wang D,Fang L,Ma J,Zhu X,Shi Y,Zhang K,Zheng H,Chen H,Li K,Xiao S

Tue Jul 30 00:00:00 UTC 2019

Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.

Frontiers in immunology

Ye X,Pan T,Wang D,Fang L,Ma J,Zhu X,Shi Y,Zhang K,Zheng H,Chen H,Li K,Xiao S

Tue Jul 30 00:00:00 UTC 2019

A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.

Journal of cell science

Fiesel FC,Moussaud-Lamodière EL,Ando M,Springer W

Fri Aug 15 00:00:00 UTC 2014

A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.

Journal of cell science

Fiesel FC,Moussaud-Lamodière EL,Ando M,Springer W

Fri Aug 15 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: E2 ubiquitin-conjugating enzyme L3; L-UBC; UbcH7; UbcM4; Ubiquitin carrier protein L3; ubiquitin conjugating enzyme E2L 3; ubiquitin-conjugating enzyme 7; Ubiquitin-conjugating enzyme E2 L3; Ubiquitin-conjugating enzyme E2-F1; ubiquitin-conjugating enzyme E2L 3; ubiquitin-conjugating enzyme UBCH7; Ubiquitin-protein ligase L3

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Gene Aliases: C79827; E2-F1; L-UBC; UBCE7; UBCH7; UbcM4; UBE2L3

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UniProt ID: (Human) P68036, (Mouse) P68037

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Entrez Gene ID: (Human) 7332, (Rat) 363836, (Mouse) 22195

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transcription coactivator activity ubiquitin-protein transferase activity protein binding ATP binding enzyme binding ubiquitin protein ligase binding poly(A) RNA binding ubiquitin conjugating enzyme activity ubiquitin-protein transferase activator activity ligase activity

protein polyubiquitination regulation of transcription, DNA-templated metabolic process cell proliferation protein ubiquitination positive regulation of protein ubiquitination protein ubiquitination involved in ubiquitin-dependent protein catabolic process cell cycle phase transition positive regulation of ubiquitin-protein transferase activity protein K11-linked ubiquitination cellular response to steroid hormone stimulus cellular response to glucocorticoid stimulus transcription, DNA-templated cellular protein modification process ubiquitin-dependent protein catabolic process positive regulation of protein targeting to mitochondrion

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