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Invitrogen
UBE2M Polyclonal Antibody
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UBE2M Antibody (PA5-118332) in WB
Western Blot using UBE2M Polyclonal Antibody (Product # PA5-118332) at 1:500 dilution. Lane A: 293T Whole Cell Lysate, Lane B: HEK-293 Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate, Lane D: U251MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa.
Product Details
PA5-118332
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived Human UBE2M fragment
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7
Contains
0.03% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2902926
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NEDD8 carrier protein; NEDD8 protein ligase; NEDD8-conjugating enzyme Ubc12; ubiquitin carrier protein M; ubiquitin conjugating enzyme E2M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog
Gene Aliases: hUbc12; UBC-RS2; UBC12; UBE2M
UniProt ID: (Human) P61081
Entrez Gene ID: (Human) 9040
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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