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Invitrogen
UBL4A Polyclonal Antibody
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UBL4A Antibody (PA5-92164) in WB
Western blot analysis of UBL4A in extracts of various cell lines. Samples were incubated with UBL4A Polyclonal antibody (Product # PA5-92164) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-92164
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-157 of human UBL4A (NP_0550501)
Conjugate
Form
Liquid
Concentration
0.11 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806396
Product Specific Information
Immunogen sequence: MQLTVKALQG RECSLQVPED ELVSTLKQLV SEKLNVPVRQ QRLLFKGKAL ADGKRLSDYS IGPNSKLNLV VKPLEKVLLE EGEAQRLADS PPPQVWQLIS KVLARHFSAA DASRVLEQLQ RDYERSLSRL TLDDIERLAS RFLHPEVTET MEKGFSK; Positive Samples: U-87MG, A-549, HeLa, Jurkat, Mouse liver, Mouse brain, Mouse heart, Mouse kidney; Cellular Location: Cytoplasm, cytosol
Target Information
UBL4A is a component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. UBL4A is the TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: housekeeping protein DXS254E; ubiquitin-like 4; ubiquitin-like 4A; Ubiquitin-like protein 4A; Ubiquitin-like protein GDX
Gene Aliases: DX254E; DXHXS254E; DXS254E; DXS254Eh; G6PD; GDX; GET5; MDY2; RGD1563983; TMA24; UBL4; UBL4A
UniProt ID: (Human) P11441, (Mouse) P21126, (Rat) B2GV38
Entrez Gene ID: (Human) 8266, (Mouse) 27643, (Rat) 293864
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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