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UBP43 Antibody (600-401-476) in WB
Western blot using Rocklands Affinity Purified anti-UBP43 antibody shows detection of a band ~43 kDa band (arrowhead) believed to be to UBP43 in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3), Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody. Signal was detected using standard techniques. The strong band between the 37kD and 50kD markers is e... View More
Product Details
600-401-476
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 25-50 of human UBP43 protein.
Conjugate
Form
Liquid
Concentration
0.9 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human UBP43 protein. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence. The core 7 amino acid sequence QPRERPR of the 10 amino acid immunogen sequence may be reactive with UBP43 from mouse and dog. This core sequence also shows partial homology with proteins other than UBP43. Cross reactivity with UBP43 homologues from other sources has not been determined.
Target Information
USP18, a member of the deubiquitinating protease family of enzymes, removes ubiquitin adducts from a broad range of protein substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 43 kDa ISG15-specific protease; hUBP43; ISG15-specific-processing protease; ubiquitin specific protease 18; Ubl carboxyl-terminal hydrolase 18; Ubl thioesterase 18; ubl thiolesterase 18
Gene Aliases: ISG43; UBP43; USP18
UniProt ID: (Human) Q9UMW8
Entrez Gene ID: (Human) 11274
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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