Proteintech

UBR5 Monoclonal Antibody (2E7A6)

UBR5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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UBR5 Antibody (66937-1-IG) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

66937-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2E7A6

Immunogen

UBR5 Fusion Protein Ag18753

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

EDD (for E3 identified by Differential Display) is a progestin-regulated gene that was isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor gene that is required for control of imaginal disc growth. EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases, or E3 enzymes. EDD has been shown to bind to ubiquitin, and like other HECT family proteins, may function as an E3 ubiquitin-protein ligase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.

Experimental eye research

Zhang J,Gao H,Jiang N,Jing M,Sun Z,Du C,Zhang J,Wang M,Li J,Gao F,Hu Y,Mu H,Cui X

Fri Jul 01 00:00:00 UTC 2022

Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.

Experimental eye research

Zhang J,Gao H,Jiang N,Jing M,Sun Z,Du C,Zhang J,Wang M,Li J,Gao F,Hu Y,Mu H,Cui X

Fri Jul 01 00:00:00 UTC 2022

Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection.

Nature communications

Gestuveo RJ,Royle J,Donald CL,Lamont DJ,Hutchinson EC,Merits A,Kohl A,Varjak M

Thu May 13 00:00:00 UTC 2021

CSN6 promotes melanoma proliferation and metastasis by controlling the UBR5-mediated ubiquitination and degradation of CDK9.

Cell death & disease

Zhang Y,Hou J,Shi S,Du J,Liu Y,Huang P,Li Q,Liu L,Hu H,Ji Y,Guo L,Shi Y,Liu Y,Cui H

Fri Jan 22 00:00:00 UTC 2021

CSN6 promotes melanoma proliferation and metastasis by controlling the UBR5-mediated ubiquitination and degradation of CDK9.

Cell death & disease

Zhang Y,Hou J,Shi S,Du J,Liu Y,Huang P,Li Q,Liu L,Hu H,Ji Y,Guo L,Shi Y,Liu Y,Cui H

Fri Jan 22 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: CH D1S3362; E3 identified by differential display; E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase, HECT domain-containing 1; ECGF1; EDG-1; EDG1; extraembryonic development; hHYD; Hyperplastic discs protein homolog; Progestin-induced protein; S1P receptor 1; S1P receptor Edg-1; S1P1; S1PR1

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Gene Aliases: DD5; EDD; EDD1; HYD; KIAA0896; Rat100; UBR5

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UniProt ID: (Human) O95071, (Mouse) Q80TP3

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Entrez Gene ID: (Human) 51366, (Rat) 117060, (Mouse) 70790

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Function(s)

RNA binding ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity ubiquitin-ubiquitin ligase activity ubiquitin binding protein domain specific binding ubiquitin protein ligase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

Process(es)

protein polyubiquitination DNA repair ubiquitin-dependent protein catabolic process cellular response to DNA damage stimulus cell proliferation positive regulation of protein import into nucleus, translocation positive regulation of catenin import into nucleus protein ubiquitination involved in ubiquitin-dependent protein catabolic process progesterone receptor signaling pathway positive regulation of canonical Wnt signaling pathway negative regulation of histone H2A K63-linked ubiquitination negative regulation of double-strand break repair protein ubiquitination

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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UBR5 Monoclonal Antibody (2E7A6)

UBR5 Antibody (66937-1-IG) in IHC (P)

Product Details

66937-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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