Proteintech

UBXN1 Polyclonal Antibody

UBXN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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UBXN1 Antibody (16135-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using (Product # 16135-1-AP) (UBXN1 antibody) at dilution of 1:1,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

16135-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

UBXN1 Fusion Protein Ag9146 (1-297 aa encoded by BC000902)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAELTALES LIEMGFPRGR AEKALALTGN QGIEAAMDWL MEHEDDPDVD EPLETPLGHI LGREPTSSEQ GGLEGSGSAA GEGKPALSEE ERQEQTKRML ELVAQKQRER EEREEREALE RERQRRRQGQ ELSAARQRLQ EDEMRRAAEE RRREKAEELA ARQRVREKIE RDKAERAKKY GGSVGSQPPP VAPEPGPVPS SPSQEPPTKR EYDQCRIQVR LPDGTSLTQT FRAREQLAAV RLYVELHRGE ELGGGQDPVQ LLSGFPRRAF SEADMERPLQ ELGLVPSAVL IVAKKCPS (1-297 aa encoded by BC000902)

Target Information

Ubiquitin-binding protein that plays a role in the modulation of innate immune response. Blocks both the RIG-I-like receptors (RLR) and NF-kappa-B pathways. Following viral infection, UBXN1 is induced and recruited to the RLR component MAVS. In turn, interferes with MAVS oligomerization, and disrupts the MAVS/TRAF3/TRAF6 signalosome. This function probably serves as a brake to prevent excessive RLR signaling (PubMed:23545497). Interferes with the TNFalpha-triggered NF-kappa-B pathway by interacting with cellular inhibitors of apoptosis proteins (cIAPs) and thereby inhibiting their recruitment to TNFR1 (PubMed:25681446). Prevents also the activation of NF-kappa-B by associating with CUL1 and thus inhibiting NF-kappa-B inhibitor alpha/NFKBIA degradation that remains bound to NF-kappa-B (PubMed:28152074). Interacts with the BRCA1-BARD1 heterodimer and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1 leads to the inhibition of the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer (PubMed:20351172). Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.

Nature communications

Ganji R,Paulo JA,Xi Y,Kline I,Zhu J,Clemen CS,Weihl CC,Purdy JG,Gygi SP,Raman M

Mon Feb 06 00:00:00 UTC 2023

A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins.

The EMBO journal

Alderson TR,Adriaenssens E,Asselbergh B,Pritišanac I,Van Lent J,Gastall HY,Wälti MA,Louis JM,Timmerman V,Baldwin AJ,Lp Benesch J

Thu Apr 15 00:00:00 UTC 2021

The p97-UBXN1 complex regulates aggresome formation.

Journal of cell science

Mukkavalli S,Klickstein JA,Ortiz B,Juo P,Raman M

Thu Apr 01 00:00:00 UTC 2021

The p97-UBXN1 complex regulates aggresome formation.

Journal of cell science

Mukkavalli S,Klickstein JA,Ortiz B,Juo P,Raman M

Thu Apr 01 00:00:00 UTC 2021

PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma.

Frontiers in immunology

Yi K,Cui X,Liu X,Wang Y,Zhao J,Yang S,Xu C,Yang E,Xiao M,Hong B,Fang C,Kang C,Tan Y,Wang Q

Wed Feb 23 00:00:00 UTC 2022

PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma.

Frontiers in immunology

Yi K,Cui X,Liu X,Wang Y,Zhao J,Yang S,Xu C,Yang E,Xiao M,Hong B,Fang C,Kang C,Tan Y,Wang Q

Wed Feb 23 00:00:00 UTC 2022

PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma.

Frontiers in immunology

Yi K,Cui X,Liu X,Wang Y,Zhao J,Yang S,Xu C,Yang E,Xiao M,Hong B,Fang C,Kang C,Tan Y,Wang Q

Wed Feb 23 00:00:00 UTC 2022

PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma.

Frontiers in immunology

Yi K,Cui X,Liu X,Wang Y,Zhao J,Yang S,Xu C,Yang E,Xiao M,Hong B,Fang C,Kang C,Tan Y,Wang Q

Wed Feb 23 00:00:00 UTC 2022

UBXN1 interacts with the S1 protein of transmissible gastroenteritis coronavirus and plays a role in viral replication.

Veterinary research

Yuan P,Huang S,Yang Z,Xie L,Wang K,Yang Y,Ran L,Yu Q,Song Z

Sat Apr 27 00:00:00 UTC 2019

The VCP-UBXN1 Complex Mediates Triage of Ubiquitylated Cytosolic Proteins Bound to the BAG6 Complex.