Invitrogen

UCP3 Polyclonal Antibody

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UCP3 Antibody (PA1-055) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of L6 (Lane 1) and C2C12 (Lane 2). The blot was probed with Anti-UCP3 Rabbit Polyclonal Antibody (Product # PA1-055, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 34 kDa band corresponding to UCP3 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® ; 12% Bis-... View More

Product Image Gallery

UCP3 Antibody — Fig. 3. Long-chain acyl-CoA dehydrogenase (LCAD) and cytochrome c protein levels greater in animals fed a high-fat diet and given AICAR than either individual treatment in the white quadriceps. A : cytochrome c protein levels in skeletal muscle ( n = 7-10). B : LCAD protein levels in skeletal muscle ( n = 7-11). C : uncoupling protein 3 (UCP3) protein levels in skeletal muscle ( n = 6-10). D : representative Western blots. Letters are used to represent significance; same letter means no significant difference ( P < 0.05). AICAR and HF were not compared. *Main treatment effect ( P < 0.05). {TM}

Product Details

PA1-055

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 26 publications 26 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C R(295) A L M K V Q V L R E S P F(308) of mouse and rat UCP-3.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2213925

Product Specific Information

PA1-055 detects uncoupling protein-3 (UCP-3) mouse, rat, and canine tissues. This antibody will also detect recombinant human UCP-3, but does not detect UCP-3 from mouse liver or kidney.

PA1-055 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~30 kDa protein representing UCP-3 from rat heart homogenate.

The PA1-055 immunogen is a synthetic peptide corresponding to residues C R(295) A L M K V Q V L R E S P F(308) of mouse and rat UPC-3. This peptide differs from the porcine and human UCP-3 sequence by a single amino acid substitution. PA1-055 immunizing peptide (Cat. # PEP-074) is available for use in neutralization and control experiments.

Target Information

The uncoupling protein UCP1 (formerly designated UCP) is an integral membrane protein unique to brown adipose tissue mitochondria. UCP1 forms a dimer that acts as a proton channel, which can uncouple oxidative phosphorylation by dissipating the electrochemical potential across the inner mitochondrial membrane. This process induces heat production in brown adipose tissue and is involved in regulation of body temperature and glucose metabolism. UCP2 is a structurally related protein that also uncouples mitochondrial respiration. It is more widely expressed in human and mouse tissues, including white adipose tissue and muscle, than is UCP1. UCP2 is thought to play a role in body weight regulation. An additional UCP family member, UCP3, is highly muscle specific and is possibly involved in the uncoupling of oxidative phosphorylation in skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Oxygen Consumption and Basal Metabolic Rate as Markers of Susceptibility to Malignant Hyperthermia and Heat Stroke.

Cells

Serano M,Pietrangelo L,Paolini C,Guarnier FA,Protasi F

PA1-055 was used in Western Blotting to suggest that metabolic rate, oxygen consumption, and body temperature at rest, all more elevated in Y522S than in CASQ1-null mice, could possibly be used as predictors of the level of susceptibility to hyperthermic crises of mice (and possibly humans).

Tue Aug 09 00:00:00 UTC 2022

Oxygen Consumption and Basal Metabolic Rate as Markers of Susceptibility to Malignant Hyperthermia and Heat Stroke.

Cells

Serano M,Pietrangelo L,Paolini C,Guarnier FA,Protasi F

Tue Aug 09 00:00:00 UTC 2022

Switching to a Standard Chow Diet at Weaning Improves the Effects of Maternal and Postnatal High-Fat and High-Sucrose Diet on Cardiometabolic Health in Adult Male Mouse Offspring.

Metabolites

Chiñas Merlin A,Gonzalez K,Mockler S,Perez Y,Jia UA,Chicco AJ,Ullevig SL,Chung E

PA1-055 was used in Protein Assay to test that switching to a standard chow diet after weaning would attenuate systemic metabolic disorders and cardiac and mitochondrial dysfunction associated with maternal and postnatal high-fat/high-sucrose (HFHS) diet in mice.

Sat Jun 18 00:00:00 UTC 2022

Nox4 mediates skeletal muscle metabolic responses to exercise.

Molecular metabolism

Specht KS,Kant S,Addington AK,McMillan RP,Hulver MW,Learnard H,Campbell M,Donnelly SR,Caliz AD,Pei Y,Reif MM,Bond JM,DeMarco A,Craige B,Keaney JF,Craige SM

PA1-055 was used in Western Blotting to demonstrate that endothelial-Nox4 is required for glucose and fatty acid oxidation, suggesting inter-tissue cross-talk between the endothelium and skeletal muscle in response to exercise.

Mon Mar 01 00:00:00 UTC 2021

Nox4 mediates skeletal muscle metabolic responses to exercise.

Molecular metabolism

Specht KS,Kant S,Addington AK,McMillan RP,Hulver MW,Learnard H,Campbell M,Donnelly SR,Caliz AD,Pei Y,Reif MM,Bond JM,DeMarco A,Craige B,Keaney JF,Craige SM

Mon Mar 01 00:00:00 UTC 2021

Cardiomyocyte-restricted high-mobility group box 1 (HMGB1) deletion leads to small heart and glycolipid metabolic disorder through GR/PGC-1α signalling.

Cell death discovery

Yu P,Liu M,Zhang B,Yu Y,Su E,Xie S,Zhang L,Yang X,Jiang H,Chen R,Zou Y,Ge J

Published figure using UCP3 polyclonal antibody (Product # PA1-055) in Western Blot

Wed Oct 28 00:00:00 UTC 2020

Cardiomyocyte-restricted high-mobility group box 1 (HMGB1) deletion leads to small heart and glycolipid metabolic disorder through GR/PGC-1α signalling.

Cell death discovery

Yu P,Liu M,Zhang B,Yu Y,Su E,Xie S,Zhang L,Yang X,Jiang H,Chen R,Zou Y,Ge J

Published figure using UCP3 polyclonal antibody (Product # PA1-055) in Western Blot

Wed Oct 28 00:00:00 UTC 2020

4-Hydroxynonenal induces Nrf2-mediated UCP3 upregulation in mouse cardiomyocytes.

Free radical biology & medicine

López-Bernardo E,Anedda A,Sánchez-Pérez P,Acosta-Iborra B,Cadenas S

PA1-055 was used in western blot to study the role of Nrf2 when mouse cardiomyocytes are treated with 4-Hydroxy-2-nonenal

Sun Nov 01 00:00:00 UTC 2015

Skeletal muscle dysfunction is associated with derangements in mitochondrial bioenergetics (but not UCP3) in a rodent model of sepsis.

American journal of physiology. Endocrinology and metabolism

Zolfaghari PS,Carré JE,Parker N,Curtin NA,Duchen MR,Singer M

PA1-055 was used in western blot to assess the metabolic, cardiovascular, and skeletal muscle contractile changes after induction of peritoneal sepsis in wild-type and Ucp3 knockout mice

Fri May 01 00:00:00 UTC 2015

Skeletal muscle dysfunction is associated with derangements in mitochondrial bioenergetics (but not UCP3) in a rodent model of sepsis.

American journal of physiology. Endocrinology and metabolism

Zolfaghari PS,Carré JE,Parker N,Curtin NA,Duchen MR,Singer M

PA1-055 was used in western blot to assess the metabolic, cardiovascular, and skeletal muscle contractile changes after induction of peritoneal sepsis in wild-type and Ucp3 knockout mice

Fri May 01 00:00:00 UTC 2015

Cardiac-specific adipose triglyceride lipase overexpression protects from cardiac steatosis and dilated cardiomyopathy following diet-induced obesity.

International journal of obesity (2005)

Pulinilkunnil T,Kienesberger PC,Nagendran J,Sharma N,Young ME,Dyck JR

PA1-055 was used in western blot to study the protective effects against cardiac steatosis and dilated cardiomyopathy of specifically overexpressing triglyceride lipase in cardiomyocytes in a murine model of diet-induced obesity

Sat Feb 01 00:00:00 UTC 2014

NF1 is a critical regulator of muscle development and metabolism.

Human molecular genetics

Sullivan K,El-Hoss J,Quinlan KG,Deo N,Garton F,Seto JT,Gdalevitch M,Turner N,Cooney GJ,Kolanczyk M,North KN,Little DG,Schindeler A

PA1-055 was used in western blot to study the role of NF1 in the development and metabolism of muscle

Sat Mar 01 00:00:00 UTC 2014

A novel SP1/SP3 dependent intronic enhancer governing transcription of the UCP3 gene in brown adipocytes.

PloS one

Hoffmann C,Zimmermann A,Hinney A,Volckmar AL,Jarrett HW,Fromme T,Klingenspor M

PA1-055 was used in western blot to study the regulation of brown adipocyte UCP3 transcription by a novel intronic enhancer that binds SP1 and SP3

Mon Aug 25 00:00:00 UTC 2014

Skeletal muscle respiratory capacity is enhanced in rats consuming an obesogenic Western diet.

American journal of physiology. Endocrinology and metabolism

Stephenson EJ,Camera DM,Jenkins TA,Kosari S,Lee JS,Hawley JA,Stepto NK

PA1-055 was used in Western Blotting to evaluate the effects of Western diet-induced obesity on skeletal muscle mitochondrial function.

Fri Jun 15 00:00:00 UTC 2012

Divergent skeletal muscle respiratory capacities in rats artificially selected for high and low running ability: a role for Nor1?

Journal of applied physiology (Bethesda, Md. : 1985)

Stephenson EJ,Stepto NK,Koch LG,Britton SL,Hawley JA

PA1-055 was used in western blot to study the potential role of Nor1 in the different metabolic profiles and capacities of rats with high and low running abilities

Thu Nov 01 00:00:00 UTC 2012

Dietary supplementation with vitamin E and C attenuates dexamethasone-induced glucose intolerance in rats.

American journal of physiology. Regulatory, integrative and comparative physiology

Williams DB,Wan Z,Frier BC,Bell RC,Field CJ,Wright DC

PA1-055 was used in western blot to study the effect of vitamin E and C on dexamethasone-induced glucose intolerance in rats

Sun Jan 01 00:00:00 UTC 2012

Exercise and PGC-1α-independent synchronization of type I muscle metabolism and vasculature by ERRγ.

Cell metabolism

Narkar VA,Fan W,Downes M,Yu RT,Jonker JW,Alaynick WA,Banayo E,Karunasiri MS,Lorca S,Evans RM

PA1-055 was used in western blot to investigate the role of ERR gamma in coordinating type I muscle metabolism and vascular transformation in muscles

Wed Mar 02 00:00:00 UTC 2011

PGC-1β deficiency accelerates the transition to heart failure in pressure overload hypertrophy.

Circulation research

Riehle C,Wende AR,Zaha VG,Pires KM,Wayment B,Olsen C,Bugger H,Buchanan J,Wang X,Moreira AB,Doenst T,Medina-Gomez G,Litwin SE,Lelliott CJ,Vidal-Puig A,Abel ED

Published figure using UCP3 polyclonal antibody (Product # PA1-055) in Western Blot

Fri Sep 16 00:00:00 UTC 2011

Amelioration of lipid-induced insulin resistance in rat skeletal muscle by overexpression of Pgc-1β involves reductions in long-chain acyl-CoA levels and oxidative stress.

Diabetologia

Wright LE,Brandon AE,Hoy AJ,Forsberg GB,Lelliott CJ,Reznick J,Löfgren L,Oscarsson J,Strömstedt M,Cooney GJ,Turner N

PA1-055 was used in western blot to investigate the ameliorative effect of Ppargc1b expression in insulin resistance in rat skeletal muscle

Wed Jun 01 00:00:00 UTC 2011

PPARδ agonists have opposing effects on insulin resistance in high fat-fed rats and mice due to different metabolic responses in muscle.

British journal of pharmacology

Ye JM,Tid-Ang J,Turner N,Zeng XY,Li HY,Cooney GJ,Wulff EM,Sauerberg P,Kraegen EW

PA1-055 was used in western blot to investigate the different effect of PPARdelta agonist on diabetic treatment between rats and mice

Wed Jun 01 00:00:00 UTC 2011

Effect of LKB1 deficiency on mitochondrial content, fibre type and muscle performance in the mouse diaphragm.

Acta physiologica (Oxford, England)

Brown JD,Hancock CR,Mongillo AD,Benjamin Barton J,DiGiovanni RA,Parcell AC,Winder WW,Thomson DM

PA1-055 was used in western blot to investigate the role of LKB1 in skeletal muscle metabolic processes

Fri Apr 01 00:00:00 UTC 2011

Exercise training reverses impaired skeletal muscle metabolism induced by artificial selection for low aerobic capacity.

American journal of physiology. Regulatory, integrative and comparative physiology

Lessard SJ,Rivas DA,Stephenson EJ,Yaspelkis BB,Koch LG,Britton SL,Hawley JA

PA1-055 was used in western blot to investigate the effect of exercise on the treatment of muscle impairment

Sat Jan 01 00:00:00 UTC 2011

Transforming growth factor β₁ oppositely regulates the hypertrophic and contractile response to β-adrenergic stimulation in the heart.

PloS one

Huntgeburth M,Tiemann K,Shahverdyan R,Schlüter KD,Schreckenberg R,Gross ML,Mödersheim S,Caglayan E,Müller-Ehmsen J,Ghanem A,Vantler M,Zimmermann WH,Böhm M,Rosenkranz S

PA1-055 was used in western blot to investigate the role of TGF-beta 1 in heart response to beta-epinephrine

Mon Apr 02 00:00:00 UTC 2012

Loss of bradykinin signaling does not accelerate the development of cardiac dysfunction in type 1 diabetic akita mice.

Endocrinology

Wende AR,Soto J,Olsen CD,Pires KM,Schell JC,Larrieu-Lahargue F,Litwin SE,Kakoki M,Takahashi N,Smithies O,Abel ED

PA1-055 was used in western blot to investigate the involvement of bradykinin signaling in diabetic cardiomyopathy

Sun Aug 01 00:00:00 UTC 2010

Chronic AMP-activated protein kinase activation and a high-fat diet have an additive effect on mitochondria in rat skeletal muscle.

Journal of applied physiology (Bethesda, Md. : 1985)

Fillmore N,Jacobs DL,Mills DB,Winder WW,Hancock CR

PA1-055 was used in western blot to study the effect of chronic AMPK activation and high-fat diet on mitochondria in rat skeletal muscle

Sun Aug 01 00:00:00 UTC 2010

GDP and carboxyatractylate inhibit 4-hydroxynonenal-activated proton conductance to differing degrees in mitochondria from skeletal muscle and heart.

Biochimica et biophysica acta

Aguirre E,Cadenas S

PA1-055 was used in western blot to investigate the differences of proton conductance mechanism between skeletal muscle and heart

Fri Oct 01 00:00:00 UTC 2010

AMP-activated protein kinase response to contractions and treatment with the AMPK activator AICAR in young adult and old skeletal muscle.

The Journal of physiology

Thomson DM,Brown JD,Fillmore N,Ellsworth SK,Jacobs DL,Winder WW,Fick CA,Gordon SE

PA1-055 was used in western blot to investigate the influence of AICAR or muscle contraction on AMPK activity in old and young adult rat muscles

Fri May 01 00:00:00 UTC 2009

Impaired skeletal muscle beta-adrenergic activation and lipolysis are associated with whole-body insulin resistance in rats bred for low intrinsic exercise capacity.

Endocrinology

Lessard SJ,Rivas DA,Chen ZP,van Denderen BJ,Watt MJ,Koch LG,Britton SL,Kemp BE,Hawley JA

PA1-055 was used in western blot to investigate the effect of beta-adrenergic signal transduction on metabolic health

Sun Nov 01 00:00:00 UTC 2009

c-Cbl-deficient mice have reduced adiposity, higher energy expenditure, and improved peripheral insulin action.

The Journal of clinical investigation

Molero JC,Jensen TE,Withers PC,Couzens M,Herzog H,Thien CB,Langdon WY,Walder K,Murphy MA,Bowtell DD,James DE,Cooney GJ

PA1-055 was used in western blot to study the role of casitas b-lineage lymphoma (c-Cbl) in the whole-body fuel homeostasis.

Mon Nov 01 00:00:00 UTC 2004

Expression of mitochondrial uncoupling protein 3 and adenine nucleotide translocase 1 genes in developing rat heart: putative involvement in control of mitochondrial membrane potential.

Journal of molecular and cellular cardiology

Skárka L,Bardová K,Brauner P,Flachs P,Jarkovská D,Kopecký J,Ostádal B

PA1-055 was used in western blot to investigate the expression of mitochondrial uncoupling protein 3 and adenine nucleotide translocase 1 genes in rat heart during development and its role in mitochondrial membrane potential

Sat Mar 01 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: mitochondrial uncoupling protein 3; Putative mitochondrial transporter UCP3; Solute carrier family 25 member 9; UCP 3; uncoupling protein; Uncoupling protein 3; Uncoupling protein 3, mitochondrial; Uncoupling protein-3

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Gene Aliases: AI645527; SLC25A9; UCP-3; UCP3

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UniProt ID: (Dog) Q9N2I9, (Mouse) P56501, (Rat) P56499

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Entrez Gene ID: (Dog) 403575, (Mouse) 22229, (Rat) 25708

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Function(s)

structural constituent of ribosome oxidative phosphorylation uncoupler activity hydrogen ion transmembrane transporter activity

Process(es)

response to superoxide response to hypoxia fatty acid metabolic process transport mitochondrial transport proton transport transmembrane transport aging response to nutrient response to cold response to activity response to insulin cellular response to hormone stimulus response to steroid hormone response to glucocorticoid hydrogen ion transmembrane transport translation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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UCP3 Polyclonal Antibody

UCP3 Antibody (PA1-055) in WB

Product Details

PA1-055

Western Blot (WB)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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