Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
UCP3 Antibody (MA5-55374) in WB
Western blot analysis of lysates from Mouse skeletal muscle, using UCP3 Recombinant Monoclonal Antibody (Product # MA5-55374) at 1:1000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.
Product Details
MA5-55374
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
10I0J9
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human UCP3.
Conjugate
Form
Liquid
Concentration
1.25 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667615
Product Specific Information
Immunogen Sequence: YDILKEKLLD YHLLTDNFPC HFVSAFGAGF CATVVASPVD VVKTRYMNSP PGQYFSPLDC MIKMVAQEGP TAFYKGFTPS FLRLGSWNVV MFVTYEQLKR A
Target Information
The uncoupling protein UCP1 (formerly designated UCP) is an integral membrane protein unique to brown adipose tissue mitochondria. UCP1 forms a dimer that acts as a proton channel, which can uncouple oxidative phosphorylation by dissipating the electrochemical potential across the inner mitochondrial membrane. This process induces heat production in brown adipose tissue and is involved in regulation of body temperature and glucose metabolism. UCP2 is a structurally related protein that also uncouples mitochondrial respiration. It is more widely expressed in human and mouse tissues, including white adipose tissue and muscle, than is UCP1. UCP2 is thought to play a role in body weight regulation. An additional UCP family member, UCP3, is highly muscle specific and is possibly involved in the uncoupling of oxidative phosphorylation in skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: mitochondrial uncoupling protein 3; Putative mitochondrial transporter UCP3; Solute carrier family 25 member 9; UCP 3; uncoupling protein; Uncoupling protein 3; uncoupling protein 3, mitochondrial
Gene Aliases: AI645527; Slc25a9; UCP-3; Ucp3
UniProt ID: (Mouse) P56501
Entrez Gene ID: (Mouse) 22229
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support