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UGT1A1 Antibody (MA5-51463) in ICC/IF
Immunocytochemistry (confocal imaging) analysis of UGT1A1 in NIH/3T3 cells. Samples were incubated with UGT1A1 Recombinant monoclonal antibody (Product # MA5-51463) using a dilution of 1:200 (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (Product # MA5-38221, dilution 1:400) (Green). DAPI was used for nuclear staining (Blue). Objective: 60x.
Product Details
MA5-51463
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4R6Q0
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of humanUGT1A1 (NP_000454.1).
Conjugate
Form
Liquid
Concentration
0.34 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3093762
Product Specific Information
Immunogen sequence: FENDSFLQRV IKTYKKIKKD SAMLLSGCSH LLHNKELMAS LAESSFDVML TDPFLPCSPI VAQYLSLPTV FFLHALPCSL EFEATQCPNP FSYVPRPLSS H
Target Information
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B1; EC 2.4.1.17; HUG BR1; HUGBR1; UDP glycosyltransferase 1 family, polypeptide A1; UDP-glucuronosyltransferase 1 family member 1; UDP-glucuronosyltransferase 1 family, member 1; UDP-glucuronosyltransferase 1 family, polypeptide A1; UDP-glucuronosyltransferase 1-1; UDP-glucuronosyltransferase 1A1; UDPGT; UDPGT 1-1; UGT 1A; UGT1 01; UGT1*1; UGT1-01; UGT1.1; UGT1A1; UGTBR1
Gene Aliases: Gnt1; Udpgt; UDPGT 1-1; Udpgt-1a; Ugt1; UGT1A01; Ugt1a1; UgtBr1
UniProt ID: (Mouse) Q63886, (Rat) Q64550
Entrez Gene ID: (Mouse) 394436, (Rat) 24861
retinoic acid binding
enzyme inhibitor activity
steroid binding
glucuronosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
enzyme binding
protein homodimerization activity
protein heterodimerization activity
metabolic process
flavonoid biosynthetic process
response to organic substance
cellular response to hormone stimulus
negative regulation of catalytic activity
flavone metabolic process
cellular glucuronidation
flavonoid glucuronidation
xenobiotic glucuronidation
biphenyl catabolic process
negative regulation of cellular glucuronidation
liver development
bilirubin conjugation
acute-phase response
response to nutrient
response to organic cyclic compound
organ regeneration
response to lipopolysaccharide
response to drug
response to starvation
response to ethanol
response to steroid hormone
response to glucocorticoid
cellular response to ethanol
cellular response to glucocorticoid stimulus
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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