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Invitrogen
UNC5B Polyclonal Antibody
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UNC5B Antibody (PA5-120319) in WB
Western blot analysis of UNC5B in extracts of various cell lines. Samples were incubated with UNC5B Polyclonal antibody (Product # PA5-120319) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-120319
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 290-377 of human UNC5B (NP_734465.2).
Conjugate
Form
Liquid
Concentration
0.99 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913891
Product Specific Information
Positive test controls include: U-87MG, A-549, 293T, Mouse brain, Mouse lung, Mouse kidney, Rat brain, Rat lung. The target is usually found in the following locations: Cell membrane, Membrane raft, Single-pass type I membrane protein.
Immunogen sequence: FQKTACTTIC PVDGAWTEWS KWSACSTECA HWRSRECMAP PPQNGGRDCS GTLLDSKNCT DGLCMQNKKT LSDPNSHLLE ASGDAALY
Target Information
Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C. elegans; Netrin receptor UNC5B; p53-regulated receptor for death and life protein 1; p53RDL1; Protein unc-5 homolog 2; Protein unc-5 homolog B; transmembrane receptor Unc5H2; unc-5 homolog 2; unc-5 homolog B; unc5 homolog 2
Gene Aliases: 6330415E02Rik; A630020F16; D10Bwg0792e; P53RDL1; UNC5B; UNC5H2; UNQ1883/PRO4326
UniProt ID: (Human) Q8IZJ1, (Mouse) Q8K1S3, (Rat) O08722
Entrez Gene ID: (Human) 219699, (Mouse) 107449, (Rat) 60630
apoptotic process
signal transduction
multicellular organism development
axon guidance
positive regulation of phosphatidylinositol 3-kinase signaling
anterior/posterior axon guidance
netrin-activated signaling pathway
negative regulation of neuron apoptotic process
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
angiogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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