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Invitrogen
USP13 Recombinant Rabbit Monoclonal Antibody (JU72-38)
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FIGURE: 1 / 5
USP13 Antibody (MA5-32802) in ICC/IF
Immunocytochemical analysis of USP13 in SH-SY5Y cells using a USP13 Monoclonal antibody (Product # MA5-32802) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
MA5-32802
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JU72-38
Immunogen
Synthetic peptide within Human USP13 aa 739-788
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2810078
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 13; Isopeptidase T-3; ISOT-3; Ubiquitin carboxyl-terminal hydrolase 13; ubiquitin specific protease 13 (isopeptidase T-3); Ubiquitin thioesterase 13; ubiquitin thiolesterase 13; Ubiquitin-specific-processing protease 13
Gene Aliases: 2700071E21Rik; AI848077; IsoT-3; ISOT3; USP13
UniProt ID: (Human) Q92995, (Mouse) Q5BKP2
Entrez Gene ID: (Human) 8975, (Mouse) 72607
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
zinc ion binding
ubiquitin protein ligase binding
ubiquitin binding
ubiquitin-like protein ligase binding
chaperone binding
proteasome binding
BAT3 complex binding
ubiquitin-specific protease activity involved in positive regulation of ERAD pathway
peptidase activity
cysteine-type peptidase activity
omega peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
metal ion binding
regulation of transcription, DNA-templated
proteolysis
ubiquitin-dependent protein catabolic process
autophagy
cell proliferation
regulation of autophagy
protein deubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
protein stabilization
regulation of proteasomal protein catabolic process
protein K63-linked deubiquitination
melanocyte differentiation
positive regulation of ERAD pathway
maintenance of unfolded protein involved in ERAD pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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