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Invitrogen
USP14 Polyclonal Antibody
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FIGURE: 1 / 9
USP14 Antibody (PA5-30300) in ICC/IF
Immunofluorescence analysis of Anti-USP14 Polyclonal Antibody (Product # PA5-30300) was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with USP14 Polyclonal Antibody (Product # PA5-30300) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary... View More
Product Details
PA5-30300
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human USP14. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547774
Product Specific Information
Recommended positive controls: HeLa, A431, Jurkat, mouse brain, rat brain, U87-MG, SK-N-SH, IMR32, SK-N-AS, K562.
Predicted reactivity: Mouse (94%), Rat (93%), Zebrafish (81%), Rabbit (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ataxia; Deubiquitinating enzyme 14; dUB-type TGT for deubiquitinating enzyme; tRNA-guanine transglycosylase, 60-kD subunit; Ubiquitin carboxyl-terminal hydrolase 14; ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase); ubiquitin specific protease 14; ubiquitin specific protease 14 (tRNA-guanine transglycosylase); Ubiquitin thioesterase 14; ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; Ubiquitin-specific-processing protease 14
Gene Aliases: 2610005K12Rik; 2610037B11Rik; AW107924; ax; C78769; nmf375; TGT; USP14
UniProt ID: (Human) P54578, (Mouse) Q9JMA1
Entrez Gene ID: (Human) 9097, (Mouse) 59025, (Rat) 291796
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
endopeptidase inhibitor activity
protein binding
tRNA guanylyltransferase activity
proteasome binding
peptidase activity
cysteine-type peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
proteolysis
ubiquitin-dependent protein catabolic process
chemical synaptic transmission
negative regulation of endopeptidase activity
protein deubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of chemotaxis
regulation of proteasomal protein catabolic process
negative regulation of ER-associated ubiquitin-dependent protein catabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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