Proteintech

USP15 Polyclonal Antibody

USP15 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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USP15 Antibody (14354-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human renal cell carcinoma tissue slide using (Product # 14354-1-AP) USP15 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

USP15 Antibody in Immunoprecipitation (IP)

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Product Details

14354-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

USP15 Fusion Protein Ag5703 (1-235 aa encoded by BC020688)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.44 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAEGGAADL DTQRSDIATL LKTSLRKGDT WYLVDSRWFK QWKKYVGFDS WDKYQMGDQN VYPGPIDNSG LLKDGDAQSL KEHLIDELDY ILLPTEGWNK LVSWYTLMEG QEPIARKVVE QGMFVKHCKV EVYLTELKLC ENGNMNNVVT RRFSKADTID TIEKEIRKIF SIPDEKETRL WNKYMSNTFE PLNKPDSTIQ DAGLYQGQVL VIEQKNEDGT WPRGPSTPKK PLEQSC (1-235 aa encoded by B C020688)

Target Information

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy.

Nature communications

Xiao X,Shi J,He C,Bu X,Sun Y,Gao M,Xiang B,Xiong W,Dai P,Mao Q,Xing X,Yao Y,Yu H,Xu G,Li S,Ren Y,Chen B,Jiang C,Meng G,Lee YR,Wei W,Freeman GJ,Xie C,Zhang J

Fri May 19 00:00:00 UTC 2023

USP8 inhibition regulates autophagy flux and controls Salmonella infection.

Frontiers in cellular and infection microbiology

Santelices J,Ou M,Maegawa GHB,Hercik K,Edelmann MJ

Mon Apr 10 00:00:00 UTC 2023

USP15 regulates p66Shc stability associated with Drp1 activation in liver ischemia/reperfusion.

Cell death & disease

Tian X,Zhao Y,Yang Z,Lu Q,Zhou L,Zheng S

Mon Sep 26 00:00:00 UTC 2022

USP15 regulates p66Shc stability associated with Drp1 activation in liver ischemia/reperfusion.

Cell death & disease

Tian X,Zhao Y,Yang Z,Lu Q,Zhou L,Zheng S

Mon Sep 26 00:00:00 UTC 2022

The deubiquitinase USP15 modulates cellular redox and is a therapeutic target in acute myeloid leukemia.

Leukemia

Niederkorn M,Ishikawa C,M Hueneman K,Bartram J,Stepanchick E,R Bennett J,E Culver-Cochran A,Bolanos LC,Uible E,Choi K,Wunderlich M,Perentesis JP,M Chlon T,Filippi MD,Starczynowski DT

Tue Feb 01 00:00:00 UTC 2022

USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.

The Science of the total environment

Zhang L,Gao X,Qin Z,Shi X,Xu K,Wang S,Tang M,Wang W,Gao S,Zuo L,Zhang L,Zhang W

Fri Aug 20 00:00:00 UTC 2021

USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.

The Science of the total environment

Zhang L,Gao X,Qin Z,Shi X,Xu K,Wang S,Tang M,Wang W,Gao S,Zuo L,Zhang L,Zhang W

Fri Aug 20 00:00:00 UTC 2021

LncRNA 2810403D21Rik/Mirf promotes ischemic myocardial injury by regulating autophagy through targeting Mir26a.

Autophagy

Liang H,Su X,Wu Q,Shan H,Lv L,Yu T,Zhao X,Sun J,Yang R,Zhang L,Yan H,Zhou Y,Li X,Du Z,Shan H

Mon Jun 01 00:00:00 UTC 2020

Overexpression of Ubiquitin-Specific Protease15 (USP15) Promotes Tumor Growth and Inhibits Apoptosis and Correlated With Poor Disease-Free Survival in Hepatocellular Carcinoma.

Technology in cancer research & treatment

Yao XQ,Li L,Piao LZ,Zhang GJ,Huang XZ,Wang Y,Liang ZL

Mon Nov 08 00:00:00 UTC 2021

Inhibition of USP15 Prevent Glutamate-Induced Oxidative Damage by Activating Nrf2/HO-1 Signaling Pathway in HT22 Cells.

Cellular and molecular neurobiology

Chen X,Bao G,Liu F

Sat Aug 01 00:00:00 UTC 2020

Disruption of the Keap1/Nrf2-Antioxidant Response System After Chronic Doxorubicin Exposure In Vivo.

Cardiovascular toxicology

Nordgren KKS,Wallace KB

Tue Dec 01 00:00:00 UTC 2020

USP15 Enhances Re-epithelialization Through Deubiquitinating EIF4A1 During Cutaneous Wound Repair.

Frontiers in cell and developmental biology

Zhao Y,Huang X,Zhang Z,Zhang Y,Zhang G,Zan T,Li Q

Mon Sep 28 00:00:00 UTC 2020

USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy.

Autophagy

Niu K,Fang H,Chen Z,Zhu Y,Tan Q,Wei D,Li Y,Balajee AS,Zhao Y

Wed Apr 01 00:00:00 UTC 2020

USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy.

Autophagy

Niu K,Fang H,Chen Z,Zhu Y,Tan Q,Wei D,Li Y,Balajee AS,Zhao Y

Wed Apr 01 00:00:00 UTC 2020

TIFAB Regulates USP15-Mediated p53 Signaling during Stressed and Malignant Hematopoiesis.

Cell reports

Niederkorn M,Hueneman K,Choi K,Varney ME,Romano L,Pujato MA,Greis KD,Inoue JI,Meetei R,Starczynowski DT

Tue Feb 25 00:00:00 UTC 2020

Ubiquitin-Specific Protease 15 Maintains Transforming Growth Factor-β Pathway Activity by Deubiquitinating Transforming Growth Factor-β Receptor I during Wound Healing.

The American journal of pathology

Zhao Y,Wang Z,Ho C,Zhang G,Li Q

Mon Jul 01 00:00:00 UTC 2019

Ubiquitin-Specific Protease 15 Maintains Transforming Growth Factor-β Pathway Activity by Deubiquitinating Transforming Growth Factor-β Receptor I during Wound Healing.

The American journal of pathology

Zhao Y,Wang Z,Ho C,Zhang G,Li Q

Mon Jul 01 00:00:00 UTC 2019

Ubiquitin-Specific Protease 15 Maintains Transforming Growth Factor-β Pathway Activity by Deubiquitinating Transforming Growth Factor-β Receptor I during Wound Healing.

The American journal of pathology

Zhao Y,Wang Z,Ho C,Zhang G,Li Q

Mon Jul 01 00:00:00 UTC 2019

Use of focused ultrasonication in activity-based profiling of deubiquitinating enzymes in tissue.

Analytical biochemistry

Nanduri B,Shack LA,Rai AN,Epperson WB,Baumgartner W,Schmidt TB,Edelmann MJ

Thu Dec 15 00:00:00 UTC 2016

Use of focused ultrasonication in activity-based profiling of deubiquitinating enzymes in tissue.

Analytical biochemistry

Nanduri B,Shack LA,Rai AN,Epperson WB,Baumgartner W,Schmidt TB,Edelmann MJ

Thu Dec 15 00:00:00 UTC 2016

USP15 stabilizes MDM2 to mediate cancer-cell survival and inhibit antitumor T cell responses.

Nature immunology

Zou Q,Jin J,Hu H,Li HS,Romano S,Xiao Y,Nakaya M,Zhou X,Cheng X,Yang P,Lozano G,Zhu C,Watowich SS,Ullrich SE,Sun SC

Sun Jun 01 00:00:00 UTC 2014

USP15 stabilizes MDM2 to mediate cancer-cell survival and inhibit antitumor T cell responses.

Nature immunology

Zou Q,Jin J,Hu H,Li HS,Romano S,Xiao Y,Nakaya M,Zhou X,Cheng X,Yang P,Lozano G,Zhu C,Watowich SS,Ullrich SE,Sun SC

Sun Jun 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Deubiquitinating enzyme 15; deubiquitinating enzyme Ubp109; granule cell antiserum positive 18; MGC131982; MGC149838; MGC74854; Ubiquitin carboxyl-terminal hydrolase 15; Ubiquitin carboxyl-terminal hydrolase of 109 kDa; ubiquitin specific protease 15; Ubiquitin thioesterase 15; ubiquitin thiolesterase 15; Ubiquitin-specific-processing protease 15; Unph-2; Unph4

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Gene Aliases: 4921514G19Rik; AI327321; E430033I05Rik; Gcap18; KIAA0529; Ubp109; UNPH-2; UNPH4; USP15

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UniProt ID: (Human) Q9Y4E8, (Mouse) Q8R5H1, (Rat) Q9R085

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Entrez Gene ID: (Human) 9958, (Mouse) 14479, (Rat) 171329

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Function(s)

cysteine-type endopeptidase activity thiol-dependent ubiquitin-specific protease activity transforming growth factor beta receptor binding protein binding identical protein binding SMAD binding ubiquitinated histone binding catalytic activity peptidase activity cysteine-type peptidase activity hydrolase activity thiol-dependent ubiquitinyl hydrolase activity

Process(es)

proteolysis ubiquitin-dependent protein catabolic process transforming growth factor beta receptor signaling pathway protein deubiquitination BMP signaling pathway monoubiquitinated protein deubiquitination histone H2B conserved C-terminal lysine deubiquitination proteasome-mediated ubiquitin-dependent protein catabolic process pathway-restricted SMAD protein phosphorylation regulation of proteasomal protein catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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USP15 Polyclonal Antibody

USP15 Antibody (14354-1-AP) in IHC (P)

Product Details

14354-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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