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Product Details
PA5-93221
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 852-952 of human USP15 (NP_0063041)
Conjugate
Form
Liquid
Concentration
1.53 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806676
Product Specific Information
Immunogen sequence: SNHYGGMGGG HYTAFAKNKD DGKWYYFDDS SVSTASEDQI VSKAAYVLFY QRQDTFSGTG FFPLDRETKG ASAATGIPLE SDEDSNDNDN DIENENCMHT N; Positive Samples: 293T, LO2; Cellular Location: Cytoplasm, Nucleus
Target Information
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 15; granule cell antiserum positive 18; MGC131982; MGC149838; MGC74854; Ubiquitin carboxyl-terminal hydrolase 15; Ubiquitin thioesterase 15; ubiquitin thiolesterase 15; Ubiquitin-specific-processing protease 15; Unph-2; Unph4
Gene Aliases: 4921514G19Rik; AI327321; E430033I05Rik; Gcap18; KIAA0529; UNPH-2; UNPH4; USP15
UniProt ID: (Human) Q9Y4E8, (Mouse) Q8R5H1
Entrez Gene ID: (Human) 9958, (Mouse) 14479
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
transforming growth factor beta receptor binding
protein binding
identical protein binding
SMAD binding
ubiquitinated histone binding
catalytic activity
peptidase activity
cysteine-type peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
proteolysis
ubiquitin-dependent protein catabolic process
transforming growth factor beta receptor signaling pathway
protein deubiquitination
BMP signaling pathway
monoubiquitinated protein deubiquitination
histone H2B conserved C-terminal lysine deubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
pathway-restricted SMAD protein phosphorylation
regulation of proteasomal protein catabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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