Proteintech

USP21 Polyclonal Antibody

USP21 Antibody in Immunocytochemistry (ICC/IF)

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USP21 Antibody (17856-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed RAW 264.7 cells using 17856-1-AP (USP21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

USP21 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

17856-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

USP21 Fusion Protein Ag12316 (216-565 aa encoded by BC090946)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: NLGNTCFLN AVLQCLSSTR PLRDFCLRRD FRQEVPGGGR AQELTEAFAD VIGALWHPDS CEAVNPTRFR AVFQKYVPSF SGYSQQDAQE FLKLLMERLH LEINRRGRRA PPILANGPVP SPPRRGGALL EEPELSDDDR ANLMWKRYLE REDSKIVDLF VGQLKSCLKC QACGYRSTTF EVFCDLSLPI PKKGFAGGKV SLRDCFNLFT KEEELESENA PVCDRCRQKT RSTKKLTVQR FPRILVLHLN RFSASRGSIK KSSVGVDFPL QRLSLGDFAS DKAGSPVYQL YALCNHSGSV HYGHYTALCR CQTGWHVYND SRVSPVSENQ VASSEGYVLF YQLMQEPPRC L (216-565 aa encoded by BC090946)

Target Information

This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

USP21 contributes to the aggressiveness of laryngeal cancer cells by deubiquitinating and stabilizing AURKA.

The Kaohsiung journal of medical sciences

Wang QD,Shi T,Xu Y,Liu Y,Zhang MJ

Sat Apr 01 00:00:00 UTC 2023

Construction and validation of a prognostic signature based on necroptosis-related genes in hepatocellular carcinoma.

PloS one

Peng YL,Wang LX,Li MY,Liu LP,Li RS

Wed Feb 22 00:00:00 UTC 2023

Salt-like transcription factor 4 promotes laryngeal cancer progression through transcriptional activation of ubiquitin-specific protease 21 to stabilize Yin Yang 1.

Pathology international

Wang QD,Liu LL,Li D,Gao L,Zhang MJ

Wed Mar 01 00:00:00 UTC 2023

Salt-like transcription factor 4 promotes laryngeal cancer progression through transcriptional activation of ubiquitin-specific protease 21 to stabilize Yin Yang 1.

Pathology international

Wang QD,Liu LL,Li D,Gao L,Zhang MJ

Wed Mar 01 00:00:00 UTC 2023

RBM47/SNHG5/FOXO3 axis activates autophagy and inhibits cell proliferation in papillary thyroid carcinoma.

Cell death & disease

Qin Y,Sun W,Wang Z,Dong W,He L,Zhang T,Lv C,Zhang H

Fri Mar 25 00:00:00 UTC 2022

Deubiquitinating Enzyme USP21 Inhibits HIV-1 Replication by Downregulating Tat Expression.

Journal of virology

Gao W,Li G,Zhao S,Wang H,Huan C,Zheng B,Jiang C,Zhang W

Thu Jun 10 00:00:00 UTC 2021

PR-619, a General Inhibitor of Deubiquitylating Enzymes, Diminishes Cisplatin Resistance in Urothelial Carcinoma Cells through the Suppression of c-Myc: An In Vitro and In Vivo Study.

International journal of molecular sciences

Hsu FS,Lin WC,Kuo KL,Chiu YL,Hsu CH,Liao SM,Dong JR,Liu SH,Chang SC,Yang SP,Chen YT,Chang RJ,Huang KH

Thu Oct 28 00:00:00 UTC 2021

Oligomerization-driven MLKL ubiquitylation antagonizes necroptosis.

The EMBO journal

Liu Z,Dagley LF,Shield-Artin K,Young SN,Bankovacki A,Wang X,Tang M,Howitt J,Stafford CA,Nachbur U,Fitzgibbon C,Garnish SE,Webb AI,Komander D,Murphy JM,Hildebrand JM,Silke J

Wed Dec 01 00:00:00 UTC 2021

The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.

Cell death & disease

Li W,Cui K,Prochownik EV,Li Y

Tue May 01 00:00:00 UTC 2018

The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.

Cell death & disease

Li W,Cui K,Prochownik EV,Li Y

Tue May 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: Deubiquitinating enzyme 21; NEDD8-specific protease; Ubiquitin carboxyl-terminal hydrolase 21; ubiquitin specific protease 21; ubiquitin specific protease 23; Ubiquitin thioesterase 21; ubiquitin thiolesterase 21; ubiquitin-specific processing protease 21; ubiquitin-specific protease 16; Ubiquitin-specific-processing protease 21

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Gene Aliases: ESTM28; PP1490; USP16; USP21; USP23; W53272

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UniProt ID: (Human) Q9UK80, (Mouse) Q9QZL6

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Entrez Gene ID: (Human) 27005, (Mouse) 30941

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Function(s)

transcription coactivator activity thiol-dependent ubiquitin-specific protease activity protein binding cysteine-type peptidase activity NEDD8-specific protease activity metal ion binding cysteine-type endopeptidase activity peptidase activity hydrolase activity thiol-dependent ubiquitinyl hydrolase activity

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated proteolysis ubiquitin-dependent protein catabolic process chromatin modification histone deubiquitination neuron projection development proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of transcription, DNA-templated regulation of proteasomal protein catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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USP21 Polyclonal Antibody

USP21 Antibody (17856-1-AP) in ICC/IF

Product Details

17856-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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