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Invitrogen
USP21 Monoclonal Antibody (16E1)
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FIGURE: 1 / 10
USP21 Antibody (MA5-34953) in ICC/IF
Immunofluorescent analysis of USP21 in 293T cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with USP21 monoclonal antibody (Product # MA5-34953) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.
Product Details
MA5-34953
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
16E1
Immunogen
Recombinant protein within Human USP21 aa 303-453
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848859
Product Specific Information
Positive Control: SH-SY5Y cell lysates, K562 cell lysates, 293T, rat seminal vesicle tissue, human liver tissue, human kidney tissue, human small intestine tissue, mouse heart tissue.
Target Information
This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Deubiquitinating enzyme 21; NEDD8-specific protease; Ubiquitin carboxyl-terminal hydrolase 21; ubiquitin specific protease 21; Ubiquitin thioesterase 21; ubiquitin thiolesterase 21; ubiquitin-specific processing protease 21; ubiquitin-specific protease 16; Ubiquitin-specific-processing protease 21
Gene Aliases: PP1490; USP16; USP21; USP23
UniProt ID: (Human) Q9UK80
Entrez Gene ID: (Human) 27005
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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