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Invitrogen
USP25 Polyclonal Antibody
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FIGURE: 1 / 1
USP25 Antibody (PA5-88642) in WB
Western blot analysis of USP25 in various lysates. Samples were incubated with USP25 Polyclonal antibody (Product # PA5-88642) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-88642
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human USP25 (NP_0375283)
Conjugate
Form
Liquid
Concentration
0.32 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805048
Product Specific Information
Immunogen sequence: MTVEQNVLQQ SAAQKHQQTF LNQLREITGI NDTQILQQAL KDSNGNLELA VAFLTAKNAK TPQQEETTYY QTALPGNDRY ISVGSQADTN VIDLTGDDKD DLQRAIALSL AESNRAFRET GITDEEQAIS RVLEASIAEN KACLKRTPTE VWRDSRNPYD RKRQDKAPVG LKNVGNTCWF SAVIQSLFNL LEFRRLVLNY KPPSNAQDLP RNQKEHRNLP FMRELRYLFA; Positive Samples: HL-60, HT1080, U251MG, HeLa, SKOV3, Mouse testis, Rat testis; Cellular Location: Cytoplasm, Nucleus
Target Information
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 25; mUSP25; Ubiquitin carboxyl-terminal hydrolase 25; ubiquitin specific protease 25; Ubiquitin thioesterase 25; ubiquitin thiolesterase 25; ubiquitin-specific processing protease 25; Ubiquitin-specific-processing protease 25; USP on chromosome 21
Gene Aliases: USP21; USP25
UniProt ID: (Human) Q9UHP3, (Mouse) P57080
Entrez Gene ID: (Human) 29761, (Mouse) 30940, (Rat) 304150
thiol-dependent ubiquitin-specific protease activity
protein binding
peptidase activity
ubiquitin protein ligase binding
SUMO binding
ubiquitin binding
ATPase binding
ubiquitin-specific protease activity involved in negative regulation of ERAD pathway
cysteine-type endopeptidase activity
cysteine-type peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
proteolysis
ubiquitin-dependent protein catabolic process
protein deubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
protein K63-linked deubiquitination
protein K48-linked deubiquitination
cellular protein modification process
negative regulation of ERAD pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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