Proteintech

USP49 Polyclonal Antibody

USP49 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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USP49 Antibody (18066-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human stomach using 18066-1-AP (USP49 antibody) at dilution of 1:50 (under 40x lens).

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Product Details

18066-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 14 publications 14 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

USP49 Fusion Protein Ag12697 (1-352 aa encoded by BC014176)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MDRCKHVGR LRLAQDHSIL NPQKWCCLEC ATTESVWACL KCSHVACGRY IEDHALKHFE ETGHPLAMEV RDLYVFCYLC KDYVLNDNPE GDLKLLRSSL LAVRGQKQDT PVRRGRTLRS MASGEDVVLP QRAPQGQPQM LTALWYRRQR LLARTLRLWF EKSSRGQAKL EQRRQEEALE RKKEEARRRR REVKRRLLEE LASTPPRKSA RLLLHTPRDA GPAASRPAAL PTSRRVPAAT LKLRRQPAMA PGVTGLRNLG NTCYMNSILQ VLSHLQKFRE CFLNLDPSKT EHLFPKATNG KTQLSGKPTN SSATELSLRN DRAEACEREG FCWNGRASIS RSLELIQNKE PSS (1-352 aa encoded by BC014176)

Target Information

Specifically deubiquitinates histone H2B at 'Lys-120' (H2BK120Ub). H2BK120Ub is a specific tag for epigenetic transcriptional activation and acts as a regulator of mRNA splicing. Deubiquitination is required for efficient cotranscriptional splicing of a large set of exons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

USP49 is a novel deubiquitylating enzyme for γ H2AX in DNA double-strand break repair.

Gene

Matsui M,Kajita S,Tsuchiya Y,Torii W,Tamekuni S,Nishi R

Sat Jul 30 00:00:00 UTC 2022

Genome-wide screening for deubiquitinase subfamily identifies ubiquitin-specific protease 49 as a novel regulator of odontogenesis.

Cell death and differentiation

Kaushal K,Kim EJ,Tyagi A,Karapurkar JK,Haq S,Jung HS,Kim KS,Ramakrishna S

Thu Sep 01 00:00:00 UTC 2022

Fbxo45 facilitates pancreatic carcinoma progression by targeting USP49 for ubiquitination and degradation.

Cell death & disease

Wu L,Yu K,Chen K,Zhu X,Yang Z,Wang Q,Gao J,Wang Y,Cao T,Xu H,Pan X,Wang L,Xia J,Li Y,Wang ZP,Ma J

Sat Mar 12 00:00:00 UTC 2022

c-MYC-USP49-BAG2 axis promotes proliferation and chemoresistance of colorectal cancer cells in vitro.

Biochemical and biophysical research communications

Tu R,Kang W,Kang Y,Chen Z,Zhang P,Xiong X,Ma J,Du RL,Zhang C

Sat Jun 04 00:00:00 UTC 2022

USP49-Mediated Histone H2B Deubiquitination Regulates HCT116 Cell Proliferation through MDM2-p53 Axis.

Molecular and cellular biology

Shi L,Shen X,Shen Y

Thu Mar 17 00:00:00 UTC 2022

USP49-Mediated Histone H2B Deubiquitination Regulates HCT116 Cell Proliferation through MDM2-p53 Axis.

Molecular and cellular biology

Shi L,Shen X,Shen Y

Thu Mar 17 00:00:00 UTC 2022

miR-5000-3p confers oxaliplatin resistance by targeting ubiquitin-specific peptidase 49 in colorectal cancer.

Cell death discovery

Zhuang YY,Zhong W,Xia ZS,Lin SZ,Chan MC,Jiang K,Li WF,Xu XY

Tue Jun 01 00:00:00 UTC 2021

CCAT1/FABP5 promotes tumour progression through mediating fatty acid metabolism and stabilizing PI3K/AKT/mTOR signalling in lung adenocarcinoma.

Journal of cellular and molecular medicine

Chen J,Alduais Y,Zhang K,Zhu X,Chen B

Fri Oct 01 00:00:00 UTC 2021

USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2.

Theranostics

Chandrasekaran AP,Kaushal K,Park CH,Kim KS,Ramakrishna S

Mon Feb 07 00:00:00 UTC 2022

Differential Expression of DUB Genes in Ovarian Cells Treated with Di-2-Ethylhexyl Phthalate.

International journal of molecular sciences

Lee DH,Park JH,Choi J,Lee KJ,Yun BS,Baek KH

Wed Mar 04 00:00:00 UTC 2020

Ubiquitin-specific protease 49 attenuates IL-1β-induced rat primary chondrocyte apoptosis by facilitating Axin deubiquitination and subsequent Wnt/β-catenin signaling cascade inhibition.

Molecular and cellular biochemistry

Yang L,Wang Z,Zou C,Mi Y,Tang H,Wu X

Sun Nov 01 00:00:00 UTC 2020

USP49 inhibits ischemia-reperfusion-induced cell viability suppression and apoptosis in human AC16 cardiomyocytes through DUSP1-JNK1/2 signaling.

Journal of cellular physiology

Zhang W,Zhang Y,Zhang H,Zhao Q,Liu Z,Xu Y

Wed May 01 00:00:00 UTC 2019

USP49 inhibits ischemia-reperfusion-induced cell viability suppression and apoptosis in human AC16 cardiomyocytes through DUSP1-JNK1/2 signaling.

Journal of cellular physiology

Zhang W,Zhang Y,Zhang H,Zhao Q,Liu Z,Xu Y

Wed May 01 00:00:00 UTC 2019

USP49 inhibits ischemia-reperfusion-induced cell viability suppression and apoptosis in human AC16 cardiomyocytes through DUSP1-JNK1/2 signaling.

Journal of cellular physiology

Zhang W,Zhang Y,Zhang H,Zhao Q,Liu Z,Xu Y

Wed May 01 00:00:00 UTC 2019

USP49 inhibits ischemia-reperfusion-induced cell viability suppression and apoptosis in human AC16 cardiomyocytes through DUSP1-JNK1/2 signaling.

Journal of cellular physiology

Zhang W,Zhang Y,Zhang H,Zhao Q,Liu Z,Xu Y

Wed May 01 00:00:00 UTC 2019

USP49 potently stabilizes APOBEC3G protein by removing ubiquitin and inhibits HIV-1 replication.

eLife

Pan T,Song Z,Wu L,Liu G,Ma X,Peng Z,Zhou M,Liang L,Liu B,Liu J,Zhang J,Zhang X,Huang R,Zhao J,Li Y,Ling X,Luo Y,Tang X,Cai W,Deng K,Li L,Zhang H

Fri Aug 09 00:00:00 UTC 2019

Ubiquitin specific peptidase 49 inhibits non-small cell lung cancer cell growth by suppressing PI3K/AKT signaling.

The Kaohsiung journal of medical sciences

Shen WM,Yin JN,Xu RJ,Xu DF,Zheng SY

Mon Jul 01 00:00:00 UTC 2019

USP49 negatively regulates cellular antiviral responses via deconjugating K63-linked ubiquitination of MITA.

PLoS pathogens

Ye L,Zhang Q,Liuyu T,Xu Z,Zhang MX,Luo MH,Zeng WB,Zhu Q,Lin D,Zhong B

Mon Apr 01 00:00:00 UTC 2019

USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.

The EMBO journal

Luo K,Li Y,Yin Y,Li L,Wu C,Chen Y,Nowsheen S,Hu Q,Zhang L,Lou Z,Yuan J

Mon May 15 00:00:00 UTC 2017

USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.

The EMBO journal

Luo K,Li Y,Yin Y,Li L,Wu C,Chen Y,Nowsheen S,Hu Q,Zhang L,Lou Z,Yuan J

Mon May 15 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Deubiquitinating enzyme 49; Ubiquitin carboxyl-terminal hydrolase 49; ubiquitin specific protease 49; Ubiquitin thioesterase 49; ubiquitin thiolesterase 49; Ubiquitin-specific-processing protease 49

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Gene Aliases: C330046L10Rik; Gm545; RGD1310513; USP49

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UniProt ID: (Human) Q70CQ1, (Mouse) Q6P9L4

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Entrez Gene ID: (Human) 25862, (Mouse) 224836, (Rat) 316211

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Function(s)

cysteine-type endopeptidase activity thiol-dependent ubiquitin-specific protease activity protein binding zinc ion binding histone binding peptidase activity cysteine-type peptidase activity hydrolase activity thiol-dependent ubiquitinyl hydrolase activity metal ion binding

Process(es)

mRNA splicing, via spliceosome mRNA processing proteolysis ubiquitin-dependent protein catabolic process RNA splicing chromatin modification protein deubiquitination histone H2B conserved C-terminal lysine deubiquitination proteasome-mediated ubiquitin-dependent protein catabolic process regulation of proteasomal protein catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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USP49 Polyclonal Antibody

USP49 Antibody (18066-1-AP) in IHC (P)

Product Details

18066-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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