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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • USP5 Antibodies

          Proteintech

          USP5 Polyclonal Antibody

          42 References
          View all (30) USP5 antibodies

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          Cite USP5 Polyclonal Antibody

          • Antibody Testing Data (14)
          USP5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          USP5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          USP5 Antibody (10473-1-AP) in IHC (P)

          Immunohistochemistry of paraffin-embedded human spleen tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 40x lens). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          USP5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          USP5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          USP5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          USP5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          USP5 Antibody in Western Blot (WB)
          USP5 Antibody in Western Blot (WB)
          USP5 Antibody in Western Blot (WB)
          USP5 Antibody in Western Blot (WB)
          USP5 Antibody in Western Blot (WB)
          USP5 Antibody in Western Blot (WB)
          USP5 Antibody in Western Blot (WB)
          USP5 Antibody in Western Blot (WB)
          USP5 Antibody in Western Blot (WB)
          USP5 Antibody in Immunoprecipitation (IP)

          Product Details

          10473-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:12,000
          View 25 publications 25 publications

          Immunohistochemistry (IHC)

          -
          View 4 publications 4 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:400
          -

          Immunocytochemistry (ICC/IF)

          -
          View 2 publications 2 publications

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 4 publications 4 publications

          Miscellaneous PubMed (MISC)

          -
          View 3 publications 3 publications

          Not applicable (N/A)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          USP5 Fusion Protein Ag0770 (1-300 aa encoded by BC004889)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.4 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: MAELSEEAL LSVLPTIRVP KAGDRVHKDE CAFSFDTPES EGGLYICMNT FLGFGKQYVE RHFNKTGQRV YLHLRRTRRP KEEDPATGTG DPPRKKPTRL AIGVEGGFDL SEEKFELDED VKIVILPDYL EIARDGLGGL PDIVRDRVTS AVEALLSADS ASRKQEVQAW DGEVRQVSKH AFSLKQLDNP ARIPPCGWKC SKCDMRENLW LNLTDGSILC GRRYFDGSGG NNHAVEHYRE TGYPLAVKLG TITPDGADVY SYDEDDMVLD PSLAEHLSHF GIDMLKMQKT DKTMTELEID M (1-300 aa encoded by BC004889)

          Target Information

          USP5 (ubiquitin carboxyl-terminal hydrolase 5) cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. It is involved in unanchored Lys-48-linked polyubiquitin disassembly, and also binds linear and Lys-63 linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Deubiquitinating enzyme 5; Isopeptidase T; testicular tissue protein Li 218; ubiquitin carboxy terminal hydrolase T; Ubiquitin carboxyl-terminal hydrolase 5; ubiquitin isopeptidase T; ubiquitin specific peptidase 5 (isopeptidase T); ubiquitin specific protease 5; ubiquitin specific protease 5 (isopeptidase T); Ubiquitin thioesterase 5; ubiquitin thiolesterase 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); Ubiquitin-specific-processing protease 5

          View more View less

          Gene Aliases: AA407472; ISOT; ISOT-1; Ucht; USP5

          View more View less

          UniProt ID: (Human) P45974, (Mouse) P56399

          View more View less

          Entrez Gene ID: (Human) 8078, (Rat) 297593, (Mouse) 22225

          View more View less

          Function(s)
          cysteine-type endopeptidase activity thiol-dependent ubiquitin-specific protease activity protein binding zinc ion binding ubiquitin binding omega peptidase activity peptidase activity cysteine-type peptidase activity hydrolase activity thiol-dependent ubiquitinyl hydrolase activity metal ion binding
          Process(es)
          protein deubiquitination positive regulation of proteasomal ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process regulation of proteasomal protein catabolic process protein K48-linked deubiquitination ubiquitin-dependent protein catabolic process proteolysis
          It has to be done as per old AB suggested Products section.
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