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Proteintech
USP5 Monoclonal Antibody (3F12C10), CoraLite® Plus 488
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Product Details
CL488-66213
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
3F12C10
Immunogen
Recombinant protein
Conjugate
Excitation/Emission Max
493/522 nm
Form
Liquid
Purification
Protein A
Storage buffer
PBS with 0.5% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, store in dark
Shipping conditions
Wet ice
Product Specific Information
Immunogen sequence: MAELSEEALL SVLPTIRVPK AGDRVHKDEC AFSFDTPESE GGLYICMNTF LGFGKQYVER HFNKTGQRVY LHLRRTRRPK EEDPATGTGD PPRKKPTRLA IGVEGGFDLS EEKFELDEDV KIVILPDYLE IARDGLGGLP DIVRDRVTSA VEALLSADSA SRKQEVQAWD GEVRQVSKHA FSLKQLDNPA RIPPCGWKCS KCDMRENLWL NLTDGSILCG RRYFDGSGGN NHAVEHYRET GYPLAVKLGT ITPDGADVYS YDEDDMVLDP SLAEHLSHFG IDMLKMQKTD KTMTELEIDM NQRIGEWELI QESGVPLKPL FGPGYTGIRN LGNSCYLNSV VQVLFSIPDF
Target Information
USP5 (ubiquitin carboxyl-terminal hydrolase 5) cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. It is involved in unanchored Lys-48-linked polyubiquitin disassembly, and also binds linear and Lys-63 linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 5; Isopeptidase T; testicular tissue protein Li 218; ubiquitin carboxy terminal hydrolase T; Ubiquitin carboxyl-terminal hydrolase 5; ubiquitin isopeptidase T; ubiquitin specific peptidase 5 (isopeptidase T); ubiquitin specific protease 5; ubiquitin specific protease 5 (isopeptidase T); Ubiquitin thioesterase 5; ubiquitin thiolesterase 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); Ubiquitin-specific-processing protease 5
Gene Aliases: AA407472; ISOT; ISOT-1; Ucht; USP5
UniProt ID: (Human) P45974, (Mouse) P56399
Entrez Gene ID: (Human) 8078, (Rat) 297593, (Mouse) 22225
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
zinc ion binding
ubiquitin binding
omega peptidase activity
peptidase activity
cysteine-type peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
metal ion binding
protein deubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
protein K48-linked deubiquitination
ubiquitin-dependent protein catabolic process
proteolysis
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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