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Bethyl Laboratories
USP7 Polyclonal Antibody
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Product Details
A300-033A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residues 1 and 50 of Human Ubiquitin Specific Peptidase 7.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, many tissues will not require epitope retrieval. However to boost weak staining epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP activity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53. Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 7; Herpes virus-associated ubiquitin-specific protease; Herpesvirus-associated ubiquitin-specific protease; mHAUSP; Ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific protease 7; ubiquitin specific protease 7 (herpes virus-associated); Ubiquitin thioesterase 7; ubiquitin thiolesterase 7; Ubiquitin-specific-processing protease 7
Gene Aliases: 2210010O09Rik; AA409944; AA617399; AU019296; AW548146; C80752; HAUSP; TEF1; USP7
UniProt ID: (Human) Q93009, (Mouse) Q6A4J8
Entrez Gene ID: (Human) 7874, (Mouse) 252870
p53 binding
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
protein C-terminus binding
transcription factor binding
ubiquitin protein ligase binding
peptidase activity
cysteine-type peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
protein homodimerization activity
DNA repair
transcription-coupled nucleotide-excision repair
regulation of transcription, DNA-templated
proteolysis
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
multicellular organism development
maintenance of DNA methylation
histone deubiquitination
protein deubiquitination
regulation of protein stability
negative regulation of NF-kappaB transcription factor activity
positive regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of sequence-specific DNA binding transcription factor activity
regulation of proteasomal protein catabolic process
viral process
protein stabilization
regulation of telomere capping
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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