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USP7 Antibody (712032) in WB
Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of A549 (Lane 1) SW480 (Lane 2), HCT 116 (Lane 3), SK-OV-3 (Lane 4) and tissue extracts of Mouse Lung (Lane 5). The blot was probed with Anti-USP7 Recombinant Rabbit Monoclonal Antibody (Product # 712032, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (product # A27036, 0.25 µg/mL, 1:4000 dilution). A 130 kDa band corresponding to USP7 was observed across the ... View More
Product Details
712032
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
5HCLC
Immunogen
Protein corresponding to human USP7 [aa537-aa664]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2734829
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Rat.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP activity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53. Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 7; Herpes virus-associated ubiquitin-specific protease; Herpesvirus-associated ubiquitin-specific protease; mHAUSP; Ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific protease 7; ubiquitin specific protease 7 (herpes virus-associated); Ubiquitin thioesterase 7; ubiquitin thiolesterase 7; Ubiquitin-specific-processing protease 7
Gene Aliases: 2210010O09Rik; AA409944; AA617399; AU019296; AW548146; C80752; HAUSP; TEF1; USP7
UniProt ID: (Human) Q93009, (Mouse) Q6A4J8
Entrez Gene ID: (Human) 7874, (Mouse) 252870
p53 binding
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
protein C-terminus binding
transcription factor binding
ubiquitin protein ligase binding
peptidase activity
cysteine-type peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
protein homodimerization activity
DNA repair
transcription-coupled nucleotide-excision repair
regulation of transcription, DNA-templated
proteolysis
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
multicellular organism development
maintenance of DNA methylation
histone deubiquitination
protein deubiquitination
regulation of protein stability
negative regulation of NF-kappaB transcription factor activity
positive regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of sequence-specific DNA binding transcription factor activity
regulation of proteasomal protein catabolic process
viral process
protein stabilization
regulation of telomere capping
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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