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Invitrogen
USP7 Recombinant Rabbit Monoclonal Antibody (JB80-36)
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Product Details
MA5-34690
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JB80-36
Immunogen
Recombinant protein within Human USP7 aa 80-180
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848598
Product Specific Information
Positive Control: 293T, rat brain tissue, human tonsil tissue, mouse colon tissue, PC-3M.
Target Information
USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP activity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53. Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 7; Herpes virus-associated ubiquitin-specific protease; herpes-virus-associated ubiquitin-specific protease; Herpesvirus-associated ubiquitin-specific protease; mHAUSP; rHAUSP; Ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific protease 7; ubiquitin specific protease 7 (herpes virus-associated); Ubiquitin thioesterase 7; ubiquitin thiolesterase 7; Ubiquitin-specific-processing protease 7
Gene Aliases: 2210010O09Rik; AA409944; AA617399; AU019296; AW548146; C80752; HAUSP; TEF1; USP7
UniProt ID: (Human) Q93009, (Rat) Q4VSI4, (Mouse) Q6A4J8
Entrez Gene ID: (Human) 7874, (Rat) 360471, (Mouse) 252870
p53 binding
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
protein C-terminus binding
transcription factor binding
ubiquitin protein ligase binding
protein homodimerization activity
peptidase activity
cysteine-type peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
transcription-coupled nucleotide-excision repair
regulation of transcription, DNA-templated
proteolysis
ubiquitin-dependent protein catabolic process
multicellular organism development
maintenance of DNA methylation
histone deubiquitination
protein deubiquitination
regulation of protein stability
negative regulation of NF-kappaB transcription factor activity
positive regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of sequence-specific DNA binding transcription factor activity
regulation of proteasomal protein catabolic process
DNA repair
cellular response to DNA damage stimulus
viral process
protein stabilization
regulation of telomere capping
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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