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Invitrogen
USP7 Monoclonal Antibody (USP 3D8), eBioscience™
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FIGURE: 1 / 3
USP7 Antibody (14-6907-82) in ICC/IF
Immunofluorescence analysis of USP7 Monoclonal Antibody was performed using 60% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 1% Triton X-100 for 30 minutes at 37730C, and blocked with 10% Normal Goat Serum for 1 hour at room temperature. The cells were labeled with 1 µg/mL of USP7 Monoclonal Antibody (Product # 14-6907-82), incubated overnight at 4°C and then labeled with 4 µg/mL of Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor ... View More
Product Details
14-6907-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
USP 3D8
Immunogen
peptide for aa1-125 human USP7
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2784638
Product Specific Information
Description: This USP 3D8 monoclonal antibody recognizes human USP7, an ubiquitin specific protease. USP 3D8 detects a ~128 kDa band corresponding to USP7 by Western Blot and stains USP7 in the nucleus in Immunocytochemistry/immunofluorescence.
Applications Reported: This USP 3D8 antibody has been reported for use in western blot and immunocytochemistry/immunofluorescence.
Applications Tested: This USP 3D8 antibody has been tested by western blot of human cell lysates and immunocytochemistry/immunofluorescence of human cell lines. This may be used at less than or equal to 2 µg/mL for western blot and less than or equal to 2 µg/mL for immunocytochemistry/immunofluorescence.
Target Information
USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP activity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53. Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 7; Herpes virus-associated ubiquitin-specific protease; Herpesvirus-associated ubiquitin-specific protease; Ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific protease 7 (herpes virus-associated); Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7
Gene Aliases: HAUSP; TEF1; USP7
UniProt ID: (Human) Q93009
Entrez Gene ID: (Human) 7874
transcription-coupled nucleotide-excision repair
ubiquitin-dependent protein catabolic process
multicellular organism development
maintenance of DNA methylation
viral process
protein deubiquitination
negative regulation of NF-kappaB transcription factor activity
protein stabilization
regulation of sequence-specific DNA binding transcription factor activity
regulation of telomere capping
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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