Proteintech

USP8 Polyclonal Antibody

View all (11) USP8 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (4)

USP8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

USP8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

USP8 Antibody (27791-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 27791-1-AP (USP8 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

USP8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

USP8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

USP8 Antibody in Western Blot (WB)

USP8 Antibody in Western Blot (WB)

Product Image Gallery

USP8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 27791-1-AP (USP8 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

USP8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 27791-1-AP (USP8 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

USP8 Antibody in Western Blot (WB) — HEK-293T cells were subjected to SDS PAGE followed by western blot with 27791-1-AP (USP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

USP8 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 27791-1-AP (USP8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Product Details

27791-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Not applicable (N/A)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

USP8 Fusion Protein Ag27143 (15-253 aa encoded by BC110590)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SSLKDLNKK TEVKPEKIST KSYVHSALKI FKTAEECRLD RDEERAYVLY MKYVTVYNLI KKRPDFKQQQ DYFHSILGPG NIKKAVEEAE RLSESLKLRY EEAEVRKKLE EKDRQEEAQR LQQKRQETGR EDGGTLAKGS LENVLDSKDK TQKSNGEKNE KCETKEKGAI TAKELYTMMT DKNISLIIMD ARRMQDYQDS CILHSLSVPE EAISPGVTAS WIEAHLPDDS KDTWKKRGNV (15-253 aa encoded by BC110590)

Target Information

USP8 is a ubiquitin specific protease that plays an important regulatory role at the level of protein turnover by preventing degradation. USP8 is involved in cell proliferation, and probably regulates the stability of STAM2 and RASGRF1. USP8 may regulate T-cell energy mediated by RNF128 via the formation of a complex containing RNF128 and STAM2. As revealed by structure/function studies, USP8 forms a ternary complex with RNF128 and OTUB1, and interacts with the SH3 domain of STAM2 and RASGRF1. Expression of USP8 is induced Upon growth stimulation in starved human fibroblasts, and expression decreases in response to growth arrest induced by cell-cell contact.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer.

Cell death and differentiation

Yang H,Zhang X,Lao M,Sun K,He L,Xu J,Duan Y,Chen Y,Ying H,Li M,Guo C,Lu Q,Wang S,Su W,Liang T,Bai X

Wed Feb 01 00:00:00 UTC 2023

Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer.

Cell death and differentiation

Yang H,Zhang X,Lao M,Sun K,He L,Xu J,Duan Y,Chen Y,Ying H,Li M,Guo C,Lu Q,Wang S,Su W,Liang T,Bai X

Wed Feb 01 00:00:00 UTC 2023

Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer.

Cell death and differentiation

Yang H,Zhang X,Lao M,Sun K,He L,Xu J,Duan Y,Chen Y,Ying H,Li M,Guo C,Lu Q,Wang S,Su W,Liang T,Bai X

Wed Feb 01 00:00:00 UTC 2023

Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer.

Cell death and differentiation

Yang H,Zhang X,Lao M,Sun K,He L,Xu J,Duan Y,Chen Y,Ying H,Li M,Guo C,Lu Q,Wang S,Su W,Liang T,Bai X

Wed Feb 01 00:00:00 UTC 2023

Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer.

Cell death and differentiation

Yang H,Zhang X,Lao M,Sun K,He L,Xu J,Duan Y,Chen Y,Ying H,Li M,Guo C,Lu Q,Wang S,Su W,Liang T,Bai X

Wed Feb 01 00:00:00 UTC 2023

Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer.

Cell death and differentiation

Yang H,Zhang X,Lao M,Sun K,He L,Xu J,Duan Y,Chen Y,Ying H,Li M,Guo C,Lu Q,Wang S,Su W,Liang T,Bai X

Wed Feb 01 00:00:00 UTC 2023

The Expression of Cell Cycle-Related Genes in USP8-Mutated Corticotroph Neuroendocrine Pituitary Tumors and Their Possible Role in Cell Cycle-Targeting Treatment.

Cancers

Mossakowska BJ,Rusetska N,Konopinski R,Kober P,Maksymowicz M,Pekul M,Zieliński G,Styk A,Kunicki J,Bujko M

Mon Nov 14 00:00:00 UTC 2022

Knockdown of USP8 inhibits prostate cancer cell growth, proliferation, and metastasis and promotes docetaxel's activity by suppressing the NF-kB signaling pathway.

Frontiers in oncology

Islam MT,Chen FZ,Chen HC,Wahid A

Sat Sep 07 00:00:00 UTC 2024

USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.

The Science of the total environment

Zhang L,Gao X,Qin Z,Shi X,Xu K,Wang S,Tang M,Wang W,Gao S,Zuo L,Zhang L,Zhang W

Fri Aug 20 00:00:00 UTC 2021

Down-regulation of USP8 Inhibits Cholangiocarcinoma Cell Proliferation and Invasion.

Cancer management and research

Jing X,Chen Y,Chen Y,Shi G,Lv S,Cheng N,Feng C,Xin Z,Zhang L,Wu J

Wed Apr 13 00:00:00 UTC 2022

Down-regulation of USP8 Inhibits Cholangiocarcinoma Cell Proliferation and Invasion.

Cancer management and research

Jing X,Chen Y,Chen Y,Shi G,Lv S,Cheng N,Feng C,Xin Z,Zhang L,Wu J

Wed Apr 13 00:00:00 UTC 2022

Exogenous H₂S Promoted USP8 Sulfhydration to Regulate Mitophagy in the Hearts of db/db Mice.

Aging and disease

Sun Y,Lu F,Yu X,Wang B,Chen J,Lu F,Peng S,Sun X,Yu M,Chen H,Wang Y,Zhang L,Liu N,Du H,Zhao D,Zhang W

Wed Apr 01 00:00:00 UTC 2020

Glucocorticoid modulatory element-binding protein 1 (GMEB1) interacts with the de-ubiquitinase USP40 to stabilize CFLAR_L and inhibit apoptosis in human non-small cell lung cancer cells.

Journal of experimental & clinical cancer research : CR

An W,Yao S,Sun X,Hou Z,Lin Y,Su L,Liu X

Thu May 02 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: Deubiquitinating enzyme 8; hUBPy; mUBPy; putative deubiquitinating enzyme; Ubiquitin carboxyl-terminal hydrolase 8; Ubiquitin isopeptidase Y; Ubiquitin thioesterase 8; ubiquitin thiolesterase 8; Ubiquitin-specific-processing protease 8

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Gene Aliases: AI574262; AW557536; HumORF8; KIAA0055; mKIAA0055; SPG59; UBPY; USP8

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UniProt ID: (Human) P40818, (Mouse) Q80U87

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Entrez Gene ID: (Human) 9101, (Mouse) 84092

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cysteine-type endopeptidase activity thiol-dependent ubiquitin-specific protease activity protein binding SH3 domain binding cadherin binding involved in cell-cell adhesion peptidase activity cysteine-type peptidase activity hydrolase activity thiol-dependent ubiquitinyl hydrolase activity

mitotic cytokinesis ubiquitin-dependent protein catabolic process endosome organization cell proliferation protein deubiquitination ERBB2 signaling pathway protein K63-linked deubiquitination protein K48-linked deubiquitination cell-cell adhesion proteolysis cell cycle Ras protein signal transduction proteasome-mediated ubiquitin-dependent protein catabolic process regulation of proteasomal protein catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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