Invitrogen

Ub-K63 Monoclonal Antibody (HWA4C4), eBioscience™

FIGURE: 1 / 3

Ub-K63 Antibody (14-6077-95) in WB

In vitro linked and purified polyubiquitin chains were analyzed by SDS-PAGE followed by immunoblotting with Anti-Human Ub-K63 Purified. The antibody detects lysine-63-linked chains (lane 1), but does not detect lysine-48-linked chains (lane 2).

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Product Details

14-6077-95

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

GST Pull Down (GPD)

View 1 publication 1 publication

Product Specifications

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

HWA4C4

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4°C

Shipping conditions

Wet ice

AB_2865190

Product Specific Information

Description: Ubiquitin is a highly conserved 76kDa protein which is ligated to many proteins in both monomeric and polymeric forms. Ubiquitin polymers (polyubiquitin) can be found in several varieties depending on which of its 7 lysine residues is the site of ligation. The two most common polymer chains are linked via an isopeptide bond between glycine-76 and either lysine-48 (K-48) or lysine-63 (K-63). K-48-linked polyubiquitination typically results in targeting the protein to the 26S proteosome resulting in proteolysis, although recently exceptions to this have been observed. In contrast, K-63-linked polyubiquitination results in signals related to intracellular trafficking, cell signaling, ribosomal biogenesis, and DNA damage repair. There are also reports that K63-linked polyubiquitin is involved in proteosome-independent proteolysis via autophagy.

HWA4C4 reacts specifically with the isopeptide linkage region of lysine-63-linked polyubiquitin. It does not react with other forms of ubiquitin including ubiquitin monomer and lysine-48-linked chains.

Applications Reported: This HWA4C4 antibody has been reported for use in immunoblotting (WB).

Applications Tested: This HWA2-8°C4 antibody has been tested by immunoblot of lysine-63-linked polyubiquitin chains. This can be used at less than or equal to 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ubiquitin is a conserved 76 amino acid polypeptide and can affect proteasomal degradation of the protein it is bound to, or mediate interactions with other proteins related to post-translational modifications. The degradation of cellular regulatory proteins by the Uubiquitin pathway is important as it controls the cellular growth and proliferation. Ubiquitin-dependent proteolysis occurs after a covalent attachment of the peptide to a lysine residue of a protein, which involves three enzymatic reactions: E1, E2 and E3. The first reaction involves ubiquitin-activating enzyme. The third reaction uses enzyme ubiquitin ligase (E3) to transfer the activated ubiquitin from E2 to a lysine residue on a protein, or directly transfers the ubiquitin from E2 to the substrate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PSGL-1 is an evolutionarily conserved antiviral restriction factor.

mBio

Jiang C,Mei M,Liu Y,Hou M,Jiao J,Tan Y,Tan X

14-6077-82 was used in Western Blot to show that this antagonism is conserved from mouse to human and from mouse retrovirus to HIV.

Tue Oct 31 00:00:00 UTC 2023

Liquid phase separation of NEMO induced by polyubiquitin chains activates NF-κB.

Molecular cell

Du M,Ea CK,Fang Y,Chen ZJ

14-6077-82 was used in Western Blot, Immunocytochemistry to demonstrate that polyUb activates IKK and NF-?B signaling by promoting the phase separation of NEMO.

Thu Jul 07 00:00:00 UTC 2022

Cholesterol alters mitophagy by impairing optineurin recruitment and lysosomal clearance in Alzheimer's disease.

Molecular neurodegeneration

Roca-Agujetas V,Barbero-Camps E,de Dios C,Podlesniy P,Abadin X,Morales A,Marí M,Trullàs R,Colell A

14-6077-80 was used in Western Blot, Immunohistochemistry to examine the impact of neuronal cholesterol changes on mitochondrial removal by autophagy.

Mon Mar 08 00:00:00 UTC 2021

Cholesterol alters mitophagy by impairing optineurin recruitment and lysosomal clearance in Alzheimer's disease.

Molecular neurodegeneration

Roca-Agujetas V,Barbero-Camps E,de Dios C,Podlesniy P,Abadin X,Morales A,Marí M,Trullàs R,Colell A

14-6077-80 was used in Western Blot, Immunohistochemistry to examine the impact of neuronal cholesterol changes on mitochondrial removal by autophagy.

Mon Mar 08 00:00:00 UTC 2021

Cholesterol alters mitophagy by impairing optineurin recruitment and lysosomal clearance in Alzheimer's disease.

Molecular neurodegeneration

Roca-Agujetas V,Barbero-Camps E,de Dios C,Podlesniy P,Abadin X,Morales A,Marí M,Trullàs R,Colell A

14-6077-80 was used in Western Blot, Immunohistochemistry to examine the impact of neuronal cholesterol changes on mitochondrial removal by autophagy.

Mon Mar 08 00:00:00 UTC 2021

Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair.

Cell

Nakazawa Y,Hara Y,Oka Y,Komine O,van den Heuvel D,Guo C,Daigaku Y,Isono M,He Y,Shimada M,Kato K,Jia N,Hashimoto S,Kotani Y,Miyoshi Y,Tanaka M,Sobue A,Mitsutake N,Suganami T,Masuda A,Ohno K,Nakada S,Mashimo T,Yamanaka K,Luijsterburg MS,Ogi T

14-6077-80 was used in Western Blot, GST Pull Down to show ubiquitination of RNAPII prevents prolonged transcription arrest and protects against neurodegeneration.

Thu Mar 19 00:00:00 UTC 2020

Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair.

Cell

14-6077-80 was used in Western Blot, GST Pull Down to show ubiquitination of RNAPII prevents prolonged transcription arrest and protects against neurodegeneration.

Thu Mar 19 00:00:00 UTC 2020

Reprogramming of Amino Acid Transporters to Support Aspartate and Glutamate Dependency Sustains Endocrine Resistance in Breast Cancer.

Cell reports

Bacci M,Lorito N,Ippolito L,Ramazzotti M,Luti S,Romagnoli S,Parri M,Bianchini F,Cappellesso F,Virga F,Gao Q,Simões BM,Marangoni E,Martin LA,Comito G,Ferracin M,Giannoni E,Mazzone M,Chiarugi P,Morandi A

14-6077-82 was used in Western Blot to provide insight into the metabolic deregulation of endocrine therapy resistant (ETR) cells, and discover molecules that may prove useful in identifying a signature for breast cancer patients with an increased risk of ETR.

Tue Jul 02 00:00:00 UTC 2019

The BEACH-containing protein WDR81 coordinates p62 and LC3C to promote aggrephagy.

The Journal of cell biology

Liu X,Li Y,Wang X,Xing R,Liu K,Gan Q,Tang C,Gao Z,Jian Y,Luo S,Guo W,Yang C

14-6077 was used in Western Blotting to report the essential role of the protein WDR81 in eliminating ubiquitinated proteins through autophagy.

Mon May 01 00:00:00 UTC 2017

Molecular Determinants of Scaffold-induced Linear Ubiquitinylation of B Cell Lymphoma/Leukemia 10 (Bcl10) during T Cell Receptor and Oncogenic Caspase Recruitment Domain-containing Protein 11 (CARD11) Signaling.

The Journal of biological chemistry

Yang YK,Yang C,Chan W,Wang Z,Deibel KE,Pomerantz JL

14-6077 was used in Western Blotting to examine the effects of Bcl10 recruitment to the CARD11 scaffold protein during T cell receptor signalling.

Fri Dec 09 00:00:00 UTC 2016

The Journal of biological chemistry

Yang YK,Yang C,Chan W,Wang Z,Deibel KE,Pomerantz JL

14-6077 was used in Western Blotting to examine the effects of Bcl10 recruitment to the CARD11 scaffold protein during T cell receptor signalling.

Fri Dec 09 00:00:00 UTC 2016

Fucose-specific DC-SIGN signalling directs T helper cell type-2 responses via IKKε- and CYLD-dependent Bcl3 activation.

Nature communications

Gringhuis SI,Kaptein TM,Wevers BA,Mesman AW,Geijtenbeek TB

14-6077 was used in Western Blotting to demonstrate that recognition of fucose-expressing extracellular pathogens by DC-SIGN favours T helper cell type-2 (TH2) responses via Bcl3.

Wed May 28 00:00:00 UTC 2014

A quantitative signaling screen identifies CARD11 mutations in the CARD and LATCH domains that induce Bcl10 ubiquitination and human lymphoma cell survival.

Molecular and cellular biology

Chan W,Schaffer TB,Pomerantz JL

14-6077 was used in Western Blotting to identify a method of detecting CARD11 mutations with oncogeneic potential, and offer a straightforward method for the discovery of variants that promote the tumorigenesis of NF-κB-dependent lymphomas.

Tue Jan 01 00:00:00 UTC 2013

Degradation of the antiviral component ARGONAUTE1 by the autophagy pathway.

Proceedings of the National Academy of Sciences of the United States of America

Derrien B,Baumberger N,Schepetilnikov M,Viotti C,De Cillia J,Ziegler-Graff V,Isono E,Schumacher K,Genschik P

14-6077 was used in Western Blotting to investigate the mechanism of P0-mediated ARGONAUTE1 degradation using a P0-inducible transgenic Arabidopsis cell line.

Tue Sep 25 00:00:00 UTC 2012

Regulation of Abro1/KIAA0157 during myocardial infarction and cell death reveals a novel cardioprotective mechanism for Lys63-specific deubiquitination.

Journal of molecular and cellular cardiology

Cilenti L,Balakrishnan MP,Wang XL,Ambivero C,Sterlicchi M,del Monte F,Ma XL,Zervos AS

14-6077-82 was used in Western Blotting to suggest a link between Lys63-specific ubiquitination, its modulation by the BRCC36-containing isopeptidase complex deubiquitinating enzyme, and heart disease development and progression.

Fri Apr 01 00:00:00 UTC 2011

Polyubiquitin linkage profiles in three models of proteolytic stress suggest the etiology of Alzheimer disease.

The Journal of biological chemistry

Dammer EB,Na CH,Xu P,Seyfried NT,Duong DM,Cheng D,Gearing M,Rees H,Lah JJ,Levey AI,Rush J,Peng J

14-6077 was used in Immunocytochemistry to demonstrate that different polyUb linkages play distinct roles under the three proteolytic stress conditions.

Fri Mar 25 00:00:00 UTC 2011

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Ub-K63 Monoclonal Antibody (HWA4C4), eBioscience™

Ub-K63 Antibody (14-6077-95) in WB

Product Details

14-6077-95

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

GST Pull Down (GPD)

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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