Invitrogen

Ubiquilin 2 Monoclonal Antibody (3D5E2)

Ubiquilin 2 Antibody in Immunocytochemistry (ICC/IF)

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Ubiquilin 2 Antibody (37-7700) in ICC/IF

Immunofluorescence analysis of Ubiquilin / Dsk2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ubiquilin / Dsk2 (3D5E2) Mouse Monoclonal Antibody (Product # 37-7700) at 2µg/mL in 0.1% BSA and incuba... View More

Ubiquilin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ubiquilin 2 Antibody in Western Blot (WB)

Ubiquilin 2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

37-7700

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:100

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

C. elegans, Human, Mouse, Yeast

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

3D5E2

Immunogen

Recombinant protein derived from an internal region of human ubiquilin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533341

Target Information

This gene encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases; and thus, are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence.

F1000Research

McDowell I,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

37-7700 was used in Immunocytochemistry to select the most appropriate antibody for their specific needs.

Wed Jun 28 00:00:00 UTC 2023

The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence.

F1000Research

McDowell I,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

37-7700 was used in Immunocytochemistry to select the most appropriate antibody for their specific needs.

Wed Jun 28 00:00:00 UTC 2023

The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence.

F1000Research

McDowell I,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

37-7700 was used in Immunocytochemistry to select the most appropriate antibody for their specific needs.

Wed Jun 28 00:00:00 UTC 2023

The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence.

F1000Research

McDowell I,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

37-7700 was used in Immunocytochemistry to select the most appropriate antibody for their specific needs.

Wed Jun 28 00:00:00 UTC 2023

Human Ubiquilin 2 and TDP-43 copathology drives neurodegeneration in transgenic Caenorhabditis elegans.

G3 (Bethesda, Md.)

Saxton AD,Kraemer BC

37-7700 was used in Western Blot to show that this model of UBQLN2-mediated neurodegeneration may be useful for further mechanistic investigation into the molecular cascades driving neurodegeneration in ALS and ALS-FTLD.

Sat Aug 07 00:00:00 UTC 2021

Overexpression of UBQLN1 reduces neuropathology in the P497S UBQLN2 mouse model of ALS/FTD.

Acta neuropathologica communications

Wang S,Tatman M,Monteiro MJ

37-7700 was used in Western Blot, Immunohistochemistry to test whether overexpression of UBQLN1 can alleviate disease in the P497S UBQLN2 mouse model of ALS/FTD by crossing transgenic (Tg) mouse lines expressing the two proteins and characterizing the resulting genotypes using a battery of pathologic and behavioral tests.

Wed Oct 07 00:00:00 UTC 2020

Overexpression of UBQLN1 reduces neuropathology in the P497S UBQLN2 mouse model of ALS/FTD.

Acta neuropathologica communications

Wang S,Tatman M,Monteiro MJ

Wed Oct 07 00:00:00 UTC 2020

Overexpression of UBQLN1 reduces neuropathology in the P497S UBQLN2 mouse model of ALS/FTD.

Acta neuropathologica communications

Wang S,Tatman M,Monteiro MJ

Wed Oct 07 00:00:00 UTC 2020

Mutation-dependent aggregation and toxicity in a Drosophila model for UBQLN2-associated ALS.

Human molecular genetics

Kim SH,Stiles SG,Feichtmeier JM,Ramesh N,Zhan L,Scalf MA,Smith LM,Pandey UB,Tibbetts RS

37-7700 was used in Immunoprecipitation to study the the normal functions of the proline-rich-repeat region and the impacts of amyotrophic lateral sclerosis-associated mutations.

Mon Jan 15 00:00:00 UTC 2018

Signature changes in ubiquilin expression in the R6/2 mouse model of Huntington's disease.

Brain research

Safren N,Chang L,Dziki KM,Monteiro MJ

37-7700 was used in immunohistochemistry and western blot to evaluate the diagnostic significance of ubiquilin expression in Huntington disease

Mon Feb 09 00:00:00 UTC 2015

Signature changes in ubiquilin expression in the R6/2 mouse model of Huntington's disease.

Brain research

Safren N,Chang L,Dziki KM,Monteiro MJ

37-7700 was used in immunohistochemistry and western blot to evaluate the diagnostic significance of ubiquilin expression in Huntington disease

Mon Feb 09 00:00:00 UTC 2015

Ubiquilin-1 overexpression increases the lifespan and delays accumulation of Huntingtin aggregates in the R6/2 mouse model of Huntington's disease.

PloS one

Safren N,El Ayadi A,Chang L,Terrillion CE,Gould TD,Boehning DF,Monteiro MJ

37-7700 was used in immunohistochemistry and western blot to test if overexpression of ubiquilin-1 delays or prevents neurodegeneration in R6/2 mice

Mon Sep 29 00:00:00 UTC 2014

Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE).

Biochimica et biophysica acta

Pitcher DS,de Mattos-Shipley K,Wang Z,Tzortzis K,Goudevenou K,Flynn H,Bohn G,Rahemtulla A,Roberts I,Snijders AP,Karadimitris A,Kleijnen MF

37-7700 was used in western blot to identify the unique SDS-PAGE pattern of nuclear proteasomes.

Mon Dec 01 00:00:00 UTC 2014

Studies of the role of ubiquitination in the interaction of ubiquilin with the loop and carboxyl terminal regions of presenilin-2.

Biochemistry

Ford DL,Monteiro MJ

Tue Jul 31 00:00:00 UTC 2007

Studies of the role of ubiquitination in the interaction of ubiquilin with the loop and carboxyl terminal regions of presenilin-2.

Biochemistry

Ford DL,Monteiro MJ

Tue Jul 31 00:00:00 UTC 2007

Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion.

The Journal of biological chemistry

Hiltunen M,Lu A,Thomas AV,Romano DM,Kim M,Jones PB,Xie Z,Kounnas MZ,Wagner SL,Berezovska O,Hyman BT,Tesco G,Bertram L,Tanzi RE

Fri Oct 27 00:00:00 UTC 2006

Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion.

The Journal of biological chemistry

Hiltunen M,Lu A,Thomas AV,Romano DM,Kim M,Jones PB,Xie Z,Kounnas MZ,Wagner SL,Berezovska O,Hyman BT,Tesco G,Bertram L,Tanzi RE

Fri Oct 27 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: Chap1; DSK2 homolog; Nedd4 binding protein 4; PLIC-2; Protein linking IAP with cytoskeleton 2; Ubiquilin-2; Ubiquitin-like product Chap1/Dsk2; Ubiquitin-related protein

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Gene Aliases: ALS15; CHAP1; DSK2; HRIHFB2157; N4BP4; Plic-2; PLIC2; UBQLN2

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UniProt ID: (Human) Q9UHD9, (Mouse) Q9QZM0

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Entrez Gene ID: (Human) 29978, (Mouse) 54609

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Function(s)

protein binding

Process(es)

regulation of macroautophagy ER-associated ubiquitin-dependent protein catabolic process negative regulation of clathrin-mediated endocytosis positive regulation of ER-associated ubiquitin-dependent protein catabolic process negative regulation of G-protein coupled receptor internalization regulation of autophagosome assembly autophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ubiquilin 2 Monoclonal Antibody (3D5E2)

Ubiquilin 2 Antibody (37-7700) in ICC/IF

Product Details

37-7700

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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