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Ubiquilin 4 Antibody (PA5-99579) in IHC (P)
Immunohistochemistry analysis of Ubiquilin 4 in mouse testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Ubiquilin 4 polyclonal antibody (Product # PA5-99579) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-99579
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human UBQLN4(Accession Q9NRR5), corresponding to amino acid residues V205-P255.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2818512
Product Specific Information
Antibody detects endogenous levels of total A1Up.
Target Information
The ubiquitin-proteosome pathway of protein degradation is one of the mechanisms that ensure the proper level of cellular proteins. The ubiquitin-like (UbL) and ubiquitin-associated (UBA) domain containing protein family is thought to be involved in proteosomal degradation. One such protein is CIP75, also known as ubiquilin-4, interacts with a number of proteins such as Ataxin-1 and Connexin-43, resulting in an increased rate of turnover of these proteins. Overexpression CIP75 led to a significant reduction of Connexin-43 half-life, with the opposite being observed in siRNA knockdown experiments. CIP75 contains an N-terminal UbL domain which is thought to interact with proteins of the 26S proteosome complex and a C-terminal UBA domain which appears to mediate its interaction with Connexin-75. At least three isoforms of CIP75 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A1Up; Ataxin-1 interacting ubiquitin-like protein; ataxin-1 ubiquitin-like interacting protein; Ataxin-1 ubiquitin-like-interacting protein A1U; CIP75; Connexin43-interacting protein of 75 kDa; RP11-336K24.8; Ubiquilin-4
Gene Aliases: A1U; A1Up; AI663987; C1orf6; CIP75; RGD1308273; UBIN; UBQLN4
UniProt ID: (Human) Q9NRR5, (Mouse) Q99NB8
Entrez Gene ID: (Human) 56893, (Mouse) 94232, (Rat) 310633
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