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          • Primary Antibodies ›
          • Ubiquitin Antibodies

          NeoBiotechnologies

          Ubiquitin Monoclonal Antibody (UBB, 1748)

          View all (59) Ubiquitin antibodies

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          Cite Ubiquitin Monoclonal Antibody (UBB, 1748)

          • Antibody Testing Data (6)
          Ubiquitin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          Ubiquitin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          FIGURE: 1 / 6

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          Ubiquitin Antibody (7314-MSM1-P1ABX) in IHC (P)

          Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human Brain using Ubiquitin Monoclonal Antibody (Product # 7314-MSM1-P1ABX). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Ubiquitin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Ubiquitin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Ubiquitin Antibody in Western Blot (WB)
          Ubiquitin Antibody in Flow Cytometry (Flow)
          Ubiquitin Antibody in Peptide array (ARRAY)
          Ubiquitin Antibody in SDS-PAGE (SDS-PAGE)

          Product Details

          7314-MSM1-P1ABX

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1-2 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          1-2 µg/mL
          -

          Flow Cytometry (Flow)

          1-2 µg/10^6 cells
          -

          Peptide Array (Array)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          All, Human

          Host/Isotype

          Mouse / IgG2c, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          UBB, 1748

          Immunogen

          Recombinant fragment of human Ubiquitin protein (around aa 1-119)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A/G

          Storage buffer

          PBS, pH 7.4

          Contains

          no preservative

          Storage conditions

          -20°C or -80°C if preferred

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

          Target Information

          Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: 40S ribosomal protein S27-like; 40S ribosomal protein S27a; CEP52; Chap1; epididymis luminal protein 112; epididymis secretory protein Li 50; hPLIC-2; HRIHFB2157; HUBCEP52; OTTHUMP00000200786; OTTHUMP00000201692; PLIC-2; polyubiquitin B; Polyubiquitin-B; Polyubiquitin-C; Ribosomal protein eS27-like; ribosomal protein S27-like; RPL40; RPS27A; RPS27L; Small ribosomal subunit protein eS27-like; UBA52; UBB; UBC; Ubiquilin 2; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein S27a; ubiquitin C; ubiquitin carboxyl extension protein 52; Ubiquitin carboxyl extension protein 80; ubiquitin-52 amino acid fusion protein; ubiquitin-CEP52; ubiquitin-CEP80; Ubiquitin-ribosomal protein eL40 fusion protein; Ubiquitin-ribosomal protein eS31 fusion protein

          View more View less

          Gene Aliases: CEP52; CEP80; HEL-S-50; HEL112; HMG20; HUBCEP52; L40; RPL40; RPS27A; RPS27L; S27A; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP2; UBCEP80

          View more View less

          UniProt ID: (Human) P62979, (Human) Q71UM5, (Human) P62987, (Human) P0CG47, (Human) P0CG48

          View more View less

          Entrez Gene ID: (Human) 6233, (Human) 51065, (Human) 7311, (Human) 7314, (Human) 7316

          View more View less

          Function(s)
          structural constituent of ribosome protein binding poly(A) RNA binding metal ion binding translation activator activity cysteine-type endopeptidase activator activity involved in apoptotic process protease binding
          Process(es)
          G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter MAPK cascade nuclear-transcribed mRNA catabolic process, nonsense-mediated decay activation of MAPK activity protein polyubiquitination nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding stimulatory C-type lectin receptor signaling pathway positive regulation of defense response to virus by host MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway glycogen biosynthetic process transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling rRNA processing translational initiation cellular protein modification process SRP-dependent cotranslational protein targeting to membrane DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest transforming growth factor beta receptor signaling pathway Notch signaling pathway I-kappaB kinase/NF-kappaB signaling JNK cascade fibroblast growth factor receptor signaling pathway regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death Wnt signaling pathway endosomal transport macroautophagy viral life cycle virion assembly viral transcription translesion synthesis negative regulation of transforming growth factor beta receptor signaling pathway anaphase-promoting complex-dependent catabolic process regulation of type I interferon production negative regulation of type I interferon production tumor necrosis factor-mediated signaling pathway nucleotide-excision repair, DNA incision ion transmembrane transport TRIF-dependent toll-like receptor signaling pathway interstrand cross-link repair NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway error-prone translesion synthesis DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability cellular protein metabolic process innate immune response positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway regulation of transcription from RNA polymerase II promoter in response to hypoxia nucleotide-binding oligomerization domain containing signaling pathway global genome nucleotide-excision repair error-free translesion synthesis intracellular transport of virus negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway xenophagy regulation of signal transduction by p53 class mediator ubiquitin homeostasis ribosomal small subunit assembly DNA repair translation activation of cysteine-type endopeptidase activity involved in apoptotic process cellular response to DNA damage stimulus mitotic G1 DNA damage checkpoint intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of translation positive regulation of protein ubiquitination mitochondrion transport along microtubule neuron projection morphogenesis regulation of mitochondrial membrane potential regulation of proteasomal protein catabolic process regulation of neuron death positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of protein monoubiquitination
          It has to be done as per old AB suggested Products section.
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