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Invitrogen
V-ATPase A Polyclonal Antibody
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V-ATPase A Antibody (PA5-89995) in WB
Western blot analysis of V-ATPase A in mouse brain. Samples were incubated with V-ATPase A Polyclonal antibody (Product # PA5-89995) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.
Product Details
PA5-89995
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human ATP6V1A (NP_0016812)
Conjugate
Form
Liquid
Concentration
0.37 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805882
Product Specific Information
Immunogen sequence: SLAETDKITL EVAKLIKDDF LQQNGYTPYD RFCPFYKTVG MLSNMIAFYD MARRAVETTA QSDNKITWSI IREHMGDILY KLSSMKFKDP LKDGEAKIKS D; Positive Samples: Mouse brain
Target Information
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 70-kDa subunit; ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha 70 kDa, isoform 2; ATPase, H+ transporting, lysosomal 70kD, V1 subunit A, isoform 1; ATPase, H+ transporting, V1 subunit A, isoform 1; ATPase, H+ transporting, V1 subunit A1; lysosomal 70kDa; V-ATPase 69 kDa subunit; V-ATPase subunit A; V-type proton ATPase catalytic subunit A; Vacuolar proton pump subunit alpha
Gene Aliases: AI647066; Atp6a1; Atp6a2; Atp6v1a; Atp6v1a1; VA68; VPP2
UniProt ID: (Mouse) P50516
Entrez Gene ID: (Mouse) 11964
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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