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Invitrogen
V-ATPase G1 Polyclonal Antibody
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V-ATPase G1 Antibody (PA5-112349) in ICC/IF
Immunocytochemichal analysis of V-ATPase G1 in PC-3 cells using a Polyclonal antibody (Product # PA5-112349) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were al... View More
Product Details
PA5-112349
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human V-type proton ATPase subunit G 1 protein (2-118AA)
Conjugate
Form
Liquid
Concentration
3 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2867085
Target Information
This protein is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase, H+ transporting, lysosomal (vacuolar proton pump), member J; ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1; DKFZp547P234; V-ATPase 13 kDa subunit 1; V-ATPase subunit G 1; V-type proton ATPase subunit G 1; vacuolar ATP synthase subunit M16; vacuolar H(+)-ATPase subunit G 1; Vacuolar proton pump subunit G 1; Vacuolar proton pump subunit M16
Gene Aliases: ATP6G; ATP6G1; ATP6GL; ATP6J; ATP6V1G1; Vma10
UniProt ID: (Human) O75348
Entrez Gene ID: (Human) 9550
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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