Product References
Engineering protein glycosylation in CHO cells to be highly similar to murine host cells.
Frontiers in bioengineering and biotechnology
Gupta S,Shah B,Fung CS,Chan PK,Wakefield DL,Kuhns S,Goudar CT,Piret JM
R960-25 was used in Immunocytochemistry to express mAbs with murine glycans to facilitate the development of biosimilars that are highly similar to marketed reference products expressed in murine cells.
Tue Mar 07 00:00:00 UTC 2023
A comprehensive SARS-CoV-2-human protein-protein interactome reveals COVID-19 pathobiology and potential host therapeutic targets.
Nature biotechnology
Zhou Y,Liu Y,Gupta S,Paramo MI,Hou Y,Mao C,Luo Y,Judd J,Wierbowski S,Bertolotti M,Nerkar M,Jehi L,Drayman N,Nicolaescu V,Gula H,Tay S,Randall G,Wang P,Lis JT,Feschotte C,Erzurum SC,Cheng F,Yu H
R960-25 was used in Western Blotting to demonstrate the value of network systems biology to understand human-virus interactions and provides hits for further research on COVID-19 therapeutics.
Sun Jan 01 00:00:00 UTC 2023
Vaccinia E5 is a major inhibitor of the DNA sensor cGAS.
Nature communications
Yang N,Wang Y,Dai P,Li T,Zierhut C,Tan A,Zhang T,Xiang JZ,Ordureau A,Funabiki H,Chen Z,Deng L
R960-25 was used in Immunoprecipitation Western Blotting to identify potential viral inhibitors of the cGAS/Stimulator of interferon gene (STING) pathway.
Mon May 22 00:00:00 UTC 2023
Vaccinia E5 is a major inhibitor of the DNA sensor cGAS.
Nature communications
Yang N,Wang Y,Dai P,Li T,Zierhut C,Tan A,Zhang T,Xiang JZ,Ordureau A,Funabiki H,Chen Z,Deng L
R960-25 was used in Immunoprecipitation Western Blotting to identify potential viral inhibitors of the cGAS/Stimulator of interferon gene (STING) pathway.
Mon May 22 00:00:00 UTC 2023
Endogenous retroviruses and TDP-43 proteinopathy form a sustaining feedback driving intercellular spread of Drosophila neurodegeneration.
Nature communications
Chang YH,Dubnau J
R960-25 was used in Immunocytochemistry-immunofluorescence to demonstrate that endogenous retrovirus (ERV) expression and TDP-43 proteinopathy are mutually reinforcing.
Tue Feb 21 00:00:00 UTC 2023
Transcription activator WCC recruits deacetylase HDA3 to control transcription dynamics and bursting in Neurospora.
Science advances
Oehler M,Geisser L,Diernfellner ACR,Brunner M
R960-25 was used in Immunoprecipitation to show that in addition to stochastic binding of transcription factors, transcription factor-mediated repression may also influence transcriptional bursting.
Fri Jun 30 00:00:00 UTC 2023
ATM signaling modulates cohesin behavior in meiotic prophase and proliferating cells.
Nature structural & molecular biology
Yu Z,Kim HJ,Dernburg AF
R960-25 was used in Immunocytochemistry to suggest that cohesin-enriched domains that promote DNA repair in mammalian cells also depend on WAPL inhibition by ATM.
Sat Apr 01 00:00:00 UTC 2023
Notch Signaling Regulates Mouse Perivascular Adipose Tissue Function via Mitochondrial Pathways.
Genes
Yang C,Yang X,Harrington A,Potts C,Kaija A,Ryzhova L,Liaw L
R960-25 was used in Western Blot to provide support for the novel concept that increased Notch signaling in the adipose tissue leads to PVAT whitening, impaired mitochondrial function, increased ferroptosis, and loss of a protective vasodilatory signal.
Fri Oct 20 00:00:00 UTC 2023
Electrical synapse structure requires distinct isoforms of a postsynaptic scaffold.
PLoS genetics
Michel JC,Grivette MMB,Harshfield AT,Huynh L,Komons AP,Loomis B,McKinnis K,Miller BT,Nguyen EQ,Huang TW,Lauf S,Michel ES,Michel ME,Kissinger JS,Marsh AJ,Crow WE,Kaye LE,Lasseigne AM,Lukowicz-Bedford RM,Farnsworth DR,Martin EA,Miller AC
R960-25 was used in Immunohistochemistry to propose that ESD molecular complexity arises both from the diversity of unique genes and from distinct isoforms encoded by single genes.
Wed Nov 01 00:00:00 UTC 2023
N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity.
Nature communications
Varland S,Silva RD,Kjosås I,Faustino A,Bogaert A,Billmann M,Boukhatmi H,Kellen B,Costanzo M,Drazic A,Osberg C,Chan K,Zhang X,Tong AHY,Andreazza S,Lee JJ,Nedyalkova L,Ušaj M,Whitworth AJ,Andrews BJ,Moffat J,Myers CL,Gevaert K,Boone C,Martinho RG,Arnesen T
R960CUS was used in Western Blot to developmental muscle defects.
Fri Oct 27 00:00:00 UTC 2023
Structure and functional determinants of Rad6-Bre1 subunits in the histone H2B ubiquitin-conjugating complex.
Nucleic acids research
Shukla PK,Bissell JE,Kumar S,Pokhrel S,Palani S,Radmall KS,Obidi O,Parnell TJ,Brasch J,Shrieve DC,Chandrasekharan MB
R960-25 was used in Western Blot to provide structural insights into Rad6-Bre1 interaction through a novel interface that is important for their biochemical and biological responses.
Tue Mar 21 00:00:00 UTC 2023
Stable endocytic structures navigate the complex pellicle of apicomplexan parasites.
Nature communications
Koreny L,Mercado-Saavedra BN,Klinger CM,Barylyuk K,Butterworth S,Hirst J,Rivera-Cuevas Y,Zaccai NR,Holzer VJC,Klingl A,Dacks JB,Carruthers VB,Robinson MS,Gras S,Waller RF
R960-25 was used in Immunocytochemistry to determine that a dominant function of endocytosis in Toxoplasma is plasma membrane homeostasis, rather than parasite nutrition, and that these specialized endocytic structures originated early in infrakingdom Alveolata likely in response to the complex cell pellicle that defines this medically and ecologically important ancient eukaryotic lineage.
Sat Apr 15 00:00:00 UTC 2023
Nanoscaled RIM clustering at presynaptic active zones revealed by endogenous tagging.
Life science alliance
Mrestani A,Dannhäuser S,Pauli M,Kollmannsberger P,Hübsch M,Morris L,Langenhan T,Heckmann M,Paul MM
R960-25 was used in Immunohistochemistry to detect RIMHA-Znf subclusters within presynaptic active zones, and provide further information on the potential of their location.
Fri Dec 01 00:00:00 UTC 2023
Wg/Wnt1 and Erasp link ER stress to proapoptotic signaling in an autosomal dominant retinitis pigmentosa model.
Experimental & molecular medicine
Park JE,Lee J,Ok S,Byun S,Chang EJ,Yoon SE,Kim YJ,Kang MJ
R960-25 was used in Western Blotting to identify a novel cell death signaling pathway involved in retinal degeneration in an autosomal dominant retinitis pigmentosa model.
Sat Jul 01 00:00:00 UTC 2023
Characterization of an Aedes ADP-Ribosylation Protein Domain and Role of Post-Translational Modification during Chikungunya Virus Infection.
Pathogens (Basel, Switzerland)
Kumar R,Mehta D,Nayak D,Sunil S
R960-25 was used in Western Blotting to demonstrate that transfection of the CHIKV nsP3 macrodomain increases the CHIKV viral titer in mosquito cells, suggesting that ADP-ribosylation may play a significant role in viral replication.
Tue May 16 00:00:00 UTC 2023
Developing a peptide to disrupt cohesin head domain interactions.
iScience
Elias M,Gani S,Lerner Y,Yamin K,Tor C,Patel A,Matityahu A,Dessau M,Qvit N,Onn I
R960-25 was used in ChIP assay to demonstrate the power of peptides to inhibit cohesin in cells and discuss the potential application of cohesin-inhibiting peptides as a therapeutic approach.
Fri Sep 15 00:00:00 UTC 2023
Developing a peptide to disrupt cohesin head domain interactions.
iScience
Elias M,Gani S,Lerner Y,Yamin K,Tor C,Patel A,Matityahu A,Dessau M,Qvit N,Onn I
R960-25 was used in ChIP assay to demonstrate the power of peptides to inhibit cohesin in cells and discuss the potential application of cohesin-inhibiting peptides as a therapeutic approach.
Fri Sep 15 00:00:00 UTC 2023
GEN-Click: Genetically Encodable Click Reactions for Spatially Restricted Metabolite Labeling.
ACS central science
Mishra PK,Sharma N,Kim H,Lee C,Rhee HW
R960-25 was used in Immunocytochemistry-immunofluorescence to develop the GEN-Click" system which can target the copper(I)-catalyzed azide/alkyne cycloaddition reaction catalysts proximal to the protein of interest and can be genetically expressed in a live cell."
Wed Aug 23 00:00:00 UTC 2023
Engineering human JMJD2A tudor domains for an improved understanding of histone peptide recognition.
Proteins
Parkinson J,Hard R,Ainsworth R,Wang W
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry to engineer the tudor domain of JMJD2A to specifically bind to H4K20me3 with a 20-fold increase of affinity and improved selectivity.
Sun Jan 01 00:00:00 UTC 2023
Soluble pathogenic tau enters brain vascular endothelial cells and drives cellular senescence and brain microvascular dysfunction in a mouse model of tauopathy.
Nature communications
Hussong SA,Banh AQ,Van Skike CE,Dorigatti AO,Hernandez SF,Hart MJ,Ferran B,Makhlouf H,Gaczynska M,Osmulski PA,McAllen SA,Dineley KT,Ungvari Z,Perez VI,Kayed R,Galvan V
R960-25 was used in Immunocytochemistry to suggest that soluble pathogenic tau aggregates mediate AD-like brain microvascular deficits in a mouse model of tauopathy, which may arise from endothelial cell senescence and eNOS dysfunction triggered by internalization of soluble tau aggregates.
Tue Apr 25 00:00:00 UTC 2023
Meiotic resetting of the cellular Sod1 pool is driven by protein aggregation, degradation, and transient LUTI-mediated repression.
The Journal of cell biology
Vander Wende HM,Gopi M,Onyundo M,Medrano C,Adanlawo T,Brar GA
R960-25 was used in Western Blot to show that one such case-Superoxide dismutase 1 (Sod1), a protein that commonly aggregates in human ALS patients-is regulated by an integrated set of events, beginning with the formation of pre-meiotic Sod1 aggregates.
Mon Mar 06 00:00:00 UTC 2023
The Ubiquitin-specific Protease USP36 Associates with the Microprocessor Complex and Regulates miRNA Biogenesis by SUMOylating DGCR8.
Cancer research communications
Li Y,Carey TS,Feng CH,Zhu HM,Sun XX,Dai MS
R960-25 was used in Western Blot to suggest that deregulated USP36-miRNA biogenesis pathway may contribute to tumorigenesis.
Wed Mar 01 00:00:00 UTC 2023
E3 ligase adaptor FBXO7 contributes to ubiquitination and proteasomal degradation of SIRT7 and promotes cell death in response to hydrogen peroxide.
The Journal of biological chemistry
Lee SH,Lee YJ,Jung S,Chung KC
R960-25 was used in Immunoprecipitation to suggest that FBXO7 negatively regulates SIRT7 stability and may suppress the cytoprotective effects of SIRT7 during H2O2-induced mammalian cell death.
Wed Mar 01 00:00:00 UTC 2023
Hypoxia truncates and constitutively activates the key cholesterol synthesis enzyme squalene monooxygenase.
eLife
Coates HW,Capell-Hattam IM,Olzomer EM,Du X,Farrell R,Yang H,Byrne FL,Brown AJ
R960-25 was used in Western Blotting to show hypoxia is a physiological trigger for this truncation, which occurs through a two-part mechanism: (1) increased targeting of SM to the proteasome via stabilization of the E3 ubiquitin ligase MARCHF6 and (2) accumulation of the SM substrate, squalene, which impedes the complete degradation of SM and liberates its truncated form.
Thu Jan 19 00:00:00 UTC 2023
Spatial position is a key determinant of N-glycan functionality of the scavenger receptor cysteine-rich domain of human hepsin.
The FEBS journal
Sun S,Hu K,Wang L,Liu M,Zhang Y,Dong N,Wu Q
R960-25 was used in Immunocytochemistry to indicate that the spatial N-glycan positioning in the SRCR domain is a key determinant in the interaction with calnexin and subsequent cell surface expression of hepsin.
Tue Aug 01 00:00:00 UTC 2023
Spatial position is a key determinant of N-glycan functionality of the scavenger receptor cysteine-rich domain of human hepsin.
The FEBS journal
Sun S,Hu K,Wang L,Liu M,Zhang Y,Dong N,Wu Q
R960-25 was used in Immunocytochemistry to indicate that the spatial N-glycan positioning in the SRCR domain is a key determinant in the interaction with calnexin and subsequent cell surface expression of hepsin.
Tue Aug 01 00:00:00 UTC 2023
A Ubiquitination Cascade Regulating the Integrated Stress Response and Survival in Carcinomas.
Cancer discovery
Cervia LD,Shibue T,Borah AA,Gaeta B,He L,Leung L,Li N,Moyer SM,Shim BH,Dumont N,Gonzalez A,Bick NR,Kazachkova M,Dempster JM,Krill-Burger JM,Piccioni F,Udeshi ND,Olive ME,Carr SA,Root DE,McFarland JM,Vazquez F,Hahn WC
R960-25 was used in Western Blot to describe the identification of a heretofore unrecognized ubiquitin ligase complex that prevents the aberrant activation of the ISR in a subset of cancer cells.
Wed Mar 01 00:00:00 UTC 2023
Separation of transcriptional repressor and activator functions in Drosophila HDAC3.
Development (Cambridge, England)
Tang M,Regadas I,Belikov S,Shilkova O,Xu L,Wernersson E,Liu X,Wu H,Bienko M,Mannervik M
R960-25 was used in Immunoprecipitation to conclude that the crucial functions of HDAC3 in embryo development involve catalytic-dependent gene activation and non-enzymatic repression by several mechanisms, including tethering of loci to the nuclear periphery.
Tue Aug 01 00:00:00 UTC 2023
Structural basis of NINJ1-mediated plasma membrane rupture in cell death.
Nature
Degen M,Santos JC,Pluhackova K,Cebrero G,Ramos S,Jankevicius G,Hartenian E,Guillerm U,Mari SA,Kohl B,Müller DJ,Schanda P,Maier T,Perez C,Sieben C,Broz P,Hiller S
R960-25 was used in Western Blotting to suggest that, during lytic cell death, the extracellular α-helices of NINJ1 insert into the plasma membrane to polymerize NINJ1 monomers into amphipathic filaments that rupture the plasma membrane.
Thu Jun 01 00:00:00 UTC 2023
The TNFR Wengen regulates the FGF pathway by an unconventional mechanism.
Nature communications
Letizia A,Espinàs ML,Giannios P,Llimargas M
R960-25 was used in Western Blot to propose that, in the tracheal context, Wengen interacts with Breathless to regulate its activity, and suggest that such unconventional mechanism, involving binding by TNFRs to unrelated proteins, may be a general strategy of TNFRs.
Thu Sep 21 00:00:00 UTC 2023
Dopamine receptor D2 regulates glioblastoma survival and death through MET and death receptor 4/5.
Neoplasia (New York, N.Y.)
Jeon HM,Oh YT,Shin YJ,Chang N,Kim D,Woo D,Yeup Y,Joo KM,Jo H,Yang H,Lee JK,Kang W,Sa J,Lee WJ,Hale J,Lathia JD,Purow B,Park MJ,Park JB,Nam DH,Lee J
R960-25 was used in Western Blot to demonstrate a molecular circuitry of oncogenic DRD2 signaling in which MET and TRAIL receptors, critical factors for tumor cell survival and cell death, respectively, govern GBM survival and death.
Mon May 01 00:00:00 UTC 2023
TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages.
Neuron
Espinosa-Medina I,Feliciano D,Belmonte-Mateos C,Linda Miyares R,Garcia-Marques J,Foster B,Lindo S,Pujades C,Koyama M,Lee T
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, Immunohistochemistry to present TEMPO (Temporal Encoding and Manipulation in a Predefined Order), an imaging-readable genetic tool allowing differential labeling and manipulation of consecutive cell generations in vertebrates.
Wed Feb 01 00:00:00 UTC 2023
Regulation of PKR-dependent RNA translation inhibition by TRIM21 upon virus infection or other stress.
PLoS pathogens
Li H,Liu S,Feng Q,Deng R,Wang J,Wang X,Tian R,Xu Y,Chen S,Liu Q,Wang L,Li X,Wan M,Peng Y,Tang S,Xue B,Zhu H
R960-25 was used in Western Blotting to highlight a previously undiscovered role of TRIM21 in regulating translation, which will provide new insights into the host antiviral response and novel targets for the treatment of translation-associated diseases in the clinic.
Thu Jun 01 00:00:00 UTC 2023
Huntingtin regulates calcium fluxes in skeletal muscle.
The Journal of general physiology
Chivet M,McCluskey M,Nicot AS,Brocard J,Beaufils M,Giovannini D,Giannesini B,Poreau B,Brocard J,Humbert S,Saudou F,Fauré J,Marty I
R960-25 was used in Immunoprecipitation to demonstrate an association of HTT with junctophilin 1 (JPH1) and stromal interaction molecule 1 (STIM1), both providing clues on the functional effects of HTT deletion on calcium fluxes.
Mon Jan 02 00:00:00 UTC 2023
CREB3L2-ATF4 heterodimerization defines a transcriptional hub of Alzheimer's disease gene expression linked to neuropathology.
Science advances
Gouveia Roque C,Chung KM,McCurdy EP,Jagannathan R,Randolph LK,Herline-Killian K,Baleriola J,Hengst U
R960-25 was used in Western Blotting to reveal differential transcription factor dimerization as a mechanism linking disease stimuli to the development of pathogenic cellular states.
Fri Mar 03 00:00:00 UTC 2023
The blood vasculature instructs lymphatic patterning in a SOX7-dependent manner.
The EMBO journal
Chiang IKN,Graus MS,Kirschnick N,Davidson T,Luu W,Harwood R,Jiang K,Li B,Wong YY,Moustaqil M,Lesieur E,Skoczylas R,Kouskoff V,Kazenwadel J,Arriola-Martinez L,Sierecki E,Gambin Y,Alitalo K,Kiefer F,Harvey NL,Francois M
R960CUS was used in ChIP assay to show that SOX7 directly binds HEY1, a canonical repressor of the Notch pathway, suggesting that transcriptional repression may also be modulated by the recruitment of this protein partner at Vegfc genomic regulatory regions.
Wed Mar 01 00:00:00 UTC 2023
The COMPASS subunit Spp1 protects nascent DNA at the Tus/Ter replication fork barrier by limiting DNA availability to nucleases.
Nature communications
Ghaddar N,Corda Y,Luciano P,Galli M,Doksani Y,Géli V
R960-25 was used in ChIP assay to propose that Spp1 limits the remodeling of the fork, which ultimately limits nascent DNA availability to nucleases.
Tue Sep 05 00:00:00 UTC 2023
The USP46 deubiquitylase complex increases Wingless/Wnt signaling strength by stabilizing Arrow/LRP6.
Nature communications
Spencer ZT,Ng VH,Benchabane H,Siddiqui GS,Duwadi D,Maines B,Bryant JM,Schwarzkopf A,Yuan K,Kassel SN,Mishra A,Pimentel A,Lebensohn AM,Rohatgi R,Gerber SA,Robbins DJ,Lee E,Ahmed Y
R960-25 was used in Immunohistochemistry to reveal an evolutionarily conserved mechanism that mediates Wnt/Wingless receptor stabilization and underlies the precise activation of signaling throughout the spatial range of the morphogen gradient.
Thu Oct 05 00:00:00 UTC 2023
Plexin-A2 enables the proliferation and the development of tumors from glioblastoma derived cells.
Cell death & disease
Toledano S,Sabag AD,Ilan N,Liburkin-Dan T,Kessler O,Neufeld G
R960-25 was used in Western Blotting to suggest that plexin-A2 may represent a novel target for the development of anti-tumorigenic therapeutics.
Thu Jan 19 00:00:00 UTC 2023
A protease and a lipoprotein jointly modulate the conserved ExoR-ExoS-ChvI signaling pathway critical in Sinorhizobium meliloti for symbiosis with legume hosts.
PLoS genetics
Bustamante JA,Ceron JS,Gao IT,Ramirez HA,Aviles MV,Bet Adam D,Brice JR,Cuellar RA,Dockery E,Jabagat MK,Karp DG,Lau JK,Li S,Lopez-Magaña R,Moore RR,Morin BKR,Nzongo J,Rezaeihaghighi Y,Sapienza-Martinez J,Tran TTK,Huang Z,Duthoy AJ,Barnett MJ,Long SR,Chen JC
R960-25 was used in Western Blot to show that two extracytoplasmic proteins, LppA (a lipoprotein) and JspA (a lipoprotein and a metalloprotease), jointly influence exopolysaccharide-I synthesis by modulating the ExoR-ExoS-ChvI pathway and expression of genes in the ChvI regulon.
Sun Oct 01 00:00:00 UTC 2023
--Atg9 interactions via its transmembrane domains are required for phagophore expansion during autophagy.
Autophagy
Chumpen Ramirez S,Gómez-Sánchez R,Verlhac P,Hardenberg R,Margheritis E,Cosentino K,Reggiori F,Ungermann C
R960-25 was used in Western Blot to reveal that interactions of Atg9 trimers via their transmembrane segments play a key role in phagophore expansion beyond Atg9's role as a lipid scramblase.
Mon May 01 00:00:00 UTC 2023
The conserved histone chaperone Spt6 is strongly required for DNA replication and genome stability.
Cell reports
Miller CLW,Winston F
R960-25 was used in Immunoprecipitation to identify Spt6 as a regulator of genome stability, at least in part through a role in DNA replication.
Tue Mar 28 00:00:00 UTC 2023
Functional classification of DDOST variants of uncertain clinical significance in congenital disorders of glycosylation.
Scientific reports
Kas SM,Mundra PA,Smith DL,Marais R
R960-25 was used in Western Blot to describe a rapid assay for functional testing of clinically relevant CDG variants to complement genome sequencing and support clinical diagnosis of affected individuals.
Tue Oct 17 00:00:00 UTC 2023
Protein kinase B/AKT phosphorylates hypoxia-inducible factor-3α1 in response to insulin, promoting cell growth and migration.
Frontiers in cell and developmental biology
Nguyen TVH,Bergmann U,Kietzmann T,Mennerich D
R960-25 was used in Western Blot to identify serine 524, located in the oxygen-dependent degradation domain of HIF-3α, as a phosphorylation site of PKB.
Fri Dec 01 00:00:00 UTC 2023
PIMT regulates hepatic gluconeogenesis in mice.
iScience
Kapadia B,Behera S,Kumar ST,Shah T,Edwin RK,Babu PP,Chakrabarti P,Parsa KVL,Misra P
R960-25 was used in Immunoprecipitation to establish that PKA regulates PIMT at post-transcriptional/translational and post-translational levels.
Fri Mar 17 00:00:00 UTC 2023
PIMT regulates hepatic gluconeogenesis in mice.
iScience
Kapadia B,Behera S,Kumar ST,Shah T,Edwin RK,Babu PP,Chakrabarti P,Parsa KVL,Misra P
R960-25 was used in Immunoprecipitation to establish that PKA regulates PIMT at post-transcriptional/translational and post-translational levels.
Fri Mar 17 00:00:00 UTC 2023
A conformation-locking inhibitor of SLC15A4 with TASL proteostatic anti-inflammatory activity.
Nature communications
Boeszoermenyi A,Bernaleau L,Chen X,Kartnig F,Xie M,Zhang H,Zhang S,Delacrétaz M,Koren A,Hopp AK,Dvorak V,Kubicek S,Aletaha D,Yang M,Rebsamen M,Heinz LX,Superti-Furga G
R960-25 was used in Immunocytochemistry to identify feeblin, a chemical entity which inhibits the nucleic acid-sensing TLR7/8 pathway activating IRF5 by disrupting the SLC15A4-TASL adapter module.
Fri Oct 20 00:00:00 UTC 2023
Antiviral type III CRISPR signalling via conjugation of ATP and SAM.
Nature
Chi H,Hoikkala V,Grüschow S,Graham S,Shirran S,White MF
R960-25 was used in Western Blot to demonstrate that the CorA-associated type III-B (Cmr) CRISPR system from Bacteroides fragilis provides immunity against mobile genetic elements when expressed in Escherichia coli.
Sun Oct 01 00:00:00 UTC 2023
Proteomic Analyses of the G Protein-Coupled Estrogen Receptor GPER1 Reveal Constitutive Links to Endoplasmic Reticulum, Glycosylation, Trafficking, and Calcium Signaling.
Cells
Ahmadian Elmi M,Motamed N,Picard D
R960-25 was used in Immunocytochemistry to show that the Ca2+ sensor STIM1 interacts with GPER1 and that upon STIM1 overexpression and depletion of Ca2+ stores, GPER1 becomes more nuclear.
Fri Nov 03 00:00:00 UTC 2023
CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signalling.
Nature communications
Serwe G,Kachaner D,Gagnon J,Plutoni C,Lajoie D,Duramé E,Sahmi M,Garrido D,Lefrançois M,Arseneault G,Saba-El-Leil MK,Meloche S,Emery G,Therrien M
R960-25 was used in Western Blotting to show that the scaffold protein CNK2 promotes cancer cell migration by coupling the pro-metastatic receptor tyrosine kinase AXL to downstream activation of ARF6 GTPase.
Thu Jun 15 00:00:00 UTC 2023
ANKRD26 is a new regulator of type I cytokine receptor signaling in normal and pathological hematopoiesis.
Haematologica
Basso-Valentina F,Donada A,Manchev VT,Lisetto M,Balayn N,Martin JE,Muller D,Oyarzun CPM,Duparc H,Arkoun B,Cumin A,Faivre L,Droin N,Biunno I,Pecci A,Balduini A,Debili N,Antony-Debré I,Marty C,Vainchenker W,Plo I,Favier R,Raslova H
R960-25 was used in Immunoprecipitation to demonstrate for the first time that ANKRD26 is expressed during the early steps of erythroid, megakaryocyte and granulocyte differentiation, and is necessary for progenitor proliferation.
Tue Aug 01 00:00:00 UTC 2023
Changing course: Glucose starvation drives nuclear accumulation of Hexokinase 2 in S. cerevisiae.
PLoS genetics
Lesko MA,Chandrashekarappa DG,Jordahl EM,Oppenheimer KG,Bowman RW,Shang C,Durrant JD,Schmidt MC,O'Donnell AF
R960-25 was used in Western Blotting to define a new model of cis- and trans-acting regulators of Hxk2 dimerization and nuclear localization.
Mon May 01 00:00:00 UTC 2023
A novel function for the sperm adhesion protein IZUMO1 in cell-cell fusion.
The Journal of cell biology
Brukman NG,Nakajima KP,Valansi C,Flyak K,Li X,Higashiyama T,Podbilewicz B
R960-25 was used in Immunocytochemistry to suggest a second function for IZUMO1 as a unilateral mouse gamete fusogen.
Mon Feb 06 00:00:00 UTC 2023
Tryptophan depletion results in tryptophan-to-phenylalanine substitutants.
Nature
Pataskar A,Champagne J,Nagel R,Kenski J,Laos M,Michaux J,Pak HS,Bleijerveld OB,Mordente K,Navarro JM,Blommaert N,Nielsen MM,Lovecchio D,Stone E,Georgiou G,de Gooijer MC,van Tellingen O,Altelaar M,Joosten RP,Perrakis A,Olweus J,Bassani-Sternberg M,Peeper DS,Agami R
R960-25 was used in Western Blot to conclude that substitutants are generated by an alternative decoding mechanism with potential effects on gene function and tumour immunoreactivity.
Tue Mar 01 00:00:00 UTC 2022
An Enhancer-Driven Stem Cell-Like Program Mediated by SOX9 Blocks Intestinal Differentiation in Colorectal Cancer.
Gastroenterology
Liang X,Duronio GN,Yang Y,Bala P,Hebbar P,Spisak S,Sahgal P,Singh H,Zhang Y,Xie Y,Cejas P,Long HW,Bass AJ,Sethi NS
R960-25 was used in Immunoprecipitation to establish SOX9 as a central regulator of an enhancerdriven stem cell-like program and carry important implications for developing therapeutics directed at overcoming differentiation defects in CRC.
Sat Jan 01 00:00:00 UTC 2022
dCas9-based gene editing for cleavage-free genomic knock-in of long sequences.
Nature cell biology
Wang C,Qu Y,Cheng JKW,Hughes NW,Zhang Q,Wang M,Cong L
R960-25 was used in Western Blot to show that dCas9-SSAP opens opportunities towards safer long-sequence genome engineering.
Tue Feb 01 00:00:00 UTC 2022
Paternal transmission of the Wolbachia CidB toxin underlies cytoplasmic incompatibility.
Current biology : CB
Horard B,Terretaz K,Gosselin-Grenet AS,Sobry H,Sicard M,Landmann F,Loppin B
R960-25 was used in Immunohistochemistry to the mechanism by which Wolbachia CI factor genes control the fate of paternal chromosomes at fertilization.
Mon Mar 28 00:00:00 UTC 2022
Identification of 14-3-3 proteins, Polo kinase, and RNA-binding protein Pes4 as key regulators of meiotic commitment in budding yeast.
Current biology : CB
Gavade JN,Puccia CM,Herod SG,Trinidad JC,Berchowitz LE,Lacefield S
R960-25 was used in Immunoprecipitation to demonstrate that meiotic commitment is actively maintained throughout meiosis, with the 14-3-3 proteins and Polo kinase serving as key regulators of this developmental program.
Mon Apr 11 00:00:00 UTC 2022
Mutant p53 elicits context-dependent pro-tumorigenic phenotypes.
Oncogene
McCann JJ,Vasilevskaya IA,McNair C,Gallagher P,Neupane NP,de Leeuw R,Shafi AA,Dylgjeri E,Mandigo AC,Schiewer MJ,Knudsen KE
R960-25 was used in Western Blot to elucidate the molecular and biological outcomes of distinct TP53 mutations and the context-dependent impact of mut-p53 in the driving of tumorigenesis.
Sat Jan 01 00:00:00 UTC 2022
Coronin 1C restricts endosomal branched actin to organize ER contact and endosome fission.
The Journal of cell biology
Striepen JF,Voeltz GK
R960CUS was used in Western Blotting to reveal how the dynamics of endosomal actin and activity of actin regulators organize ER-associated bud fission.
Mon Aug 01 00:00:00 UTC 2022
The US3 Kinase of Herpes Simplex Virus Phosphorylates the RNA Sensor RIG-I To Suppress Innate Immunity.
Journal of virology
van Gent M,Chiang JJ,Muppala S,Chiang C,Azab W,Kattenhorn L,Knipe DM,Osterrieder N,Gack MU
R960-25 was used in Western Blot to identify new therapeutic targets for HSV-1-induced diseases.
Wed Feb 23 00:00:00 UTC 2022
Biochemical analysis of novel NAA10 variants suggests distinct pathogenic mechanisms involving impaired protein N-terminal acetylation.
Human genetics
McTiernan N,Tranebjærg L,Bjørheim AS,Hogue JS,Wilson WG,Schmidt B,Boerrigter MM,Nybo ML,Smeland MF,Tümer Z,Arnesen T
R960-25 was used in Immunoprecipitation to present eight individuals from five families with five different de novo or inherited NAA10 variants.
Mon Aug 01 00:00:00 UTC 2022
Evidence for a Dual-Pathway, 2-Hit Genetic Model for Focal Cortical Dysplasia and Epilepsy.
Neurology. Genetics
Bennett MF,Hildebrand MS,Kayumi S,Corbett MA,Gupta S,Ye Z,Krivanek M,Burgess R,Henry OJ,Damiano JA,Boys A,Gécz J,Bahlo M,Scheffer IE,Berkovic SF
R960-25 was used in Western Blot to investigate the 2-hit model by performing genetic analysis and searching for germline and somatic variants in genes in the mTOR and related pathways.
Tue Feb 01 00:00:00 UTC 2022
Extensive conformational and physical plasticity protects HER2-HER3 tumorigenic signaling.
Cell reports
Campbell MR,Ruiz-Saenz A,Zhang Y,Peterson E,Steri V,Oeffinger J,Sampang M,Jura N,Moasser MM
R960-25 was used in Western Blot to reveal complete uncoupling of intracellular signaling and tumorigenic function from regulation or constraints from their extracellular domains (ECDs).
Tue Feb 01 00:00:00 UTC 2022
PCR-based profiling of transcription factor activity in vivo by a virus-based reporter battery.
iScience
Abe H,Abe K
R960-25 was used in Immunohistochemistry to introduce a viral-vector-based TF reporter battery that can be used to simultaneously analyze the activity of multiple TFs, visualized as the TF activity profile (TFAP) obtained by qPCR.
Fri Mar 18 00:00:00 UTC 2022
SARS-CoV-2 Nsp14 protein associates with IMPDH2 and activates NF-κB signaling.
Frontiers in immunology
Li TW,Kenney AD,Park JG,Fiches GN,Liu H,Zhou D,Biswas A,Zhao W,Que J,Santoso N,Martinez-Sobrido L,Yount JS,Zhu J
R960-25 was used in Western Blotting to study how the SARS-CoV-2 Nsp14 protein contributes to the viral activation of NF-κB signaling through Inosine-5'-monophosphate dehydrogenase 2 (IMPDH2) and the induction of pro-inflammatory cytokines.
Mon Oct 03 00:00:00 UTC 2022
Cell type-specific biotin labeling in vivo resolves regional neuronal and astrocyte proteomic differences in mouse brain.
Nature communications
Rayaprolu S,Bitarafan S,Santiago JV,Betarbet R,Sunna S,Cheng L,Xiao H,Nelson RS,Kumar P,Bagchi P,Duong DM,Goettemoeller AM,Oláh VJ,Rowan M,Levey AI,Wood LB,Seyfried NT,Rangaraju S
R960-25 was used in Functional assay to describe a mouse line for cell type-specific expression of biotin ligase TurboID, for in vivo biotinylation of proteins.
Wed May 25 00:00:00 UTC 2022
LUBAC assembles a ubiquitin signaling platform at mitochondria for signal amplification and transport of NF-κB to the nucleus.
The EMBO journal
Wu Z,Berlemann LA,Bader V,Sehr DA,Dawin E,Covallero A,Meschede J,Angersbach L,Showkat C,Michaelis JB,Münch C,Rieger B,Namgaladze D,Herrera MG,Fiesel FC,Springer W,Mendes M,Stepien J,Barkovits K,Marcus K,Sickmann A,Dittmar G,Busch KB,Riedel D,Brini M,Tatzelt J,Cali T,Winklhofer KF
R960-25 was used in Immunoprecipitation to reveal a role for mitochondria in amplifying TNF-mediated NF-κB activation, both serving as a signaling platform, as well as a transport mode for activated NF-κB to the nuclear.
Thu Dec 15 00:00:00 UTC 2022
Integrated screens uncover a cell surface tumor suppressor gene KIRREL involved in Hippo pathway.
Proceedings of the National Academy of Sciences of the United States of America
Wang C,Feng X,Su D,Chen Z,Wang S,Tang M,Huang M,Nie L,Zhang H,Li S,Yin L,Johnson RL,Hart T,Chen J
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot to identify a tumor suppressor gene, KIRREL, that acts in the Hippo signaling pathway.
Tue Jun 21 00:00:00 UTC 2022
MuRF1 deficiency prevents age-related fat weight gain, possibly through accumulation of PDK4 in skeletal muscle mitochondria in older mice.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Sugiura K,Hirasaka K,Maeda T,Uchida T,Kishimoto K,Oarada M,Labeit S,Ulla A,Sakakibara I,Nakao R,Sairyo K,Nikawa T
R960-25 was used in Western Blotting to show that MuRF1 deficiency enhances lipid metabolism by upregulating PDK4 localization into skeletal muscle mitochondria - a potential therapeutic target for age-related dysfunction of lipid metabolism and muscle atrophy.
Sun May 01 00:00:00 UTC 2022
Repression of essential cell cycle genes increases cellular fitness.
PLoS genetics
Conti MM,Ghizzoni JM,Gil-Bona A,Wang W,Costanzo M,Li R,Flynn MJ,Zhu LJ,Myers CL,Boone C,Andrews BJ,Benanti JA
R960-25 was used in Western Blotting to reveal how altering expression of cell cycle genes leads to a redistribution of cell cycle timing and confers a fitness advantage to cells.
Mon Aug 01 00:00:00 UTC 2022
A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency.
Cell reports
Huang X,Bashkenova N,Hong Y,Lyu C,Guallar D,Hu Z,Malik V,Li D,Wang H,Shen X,Zhou H,Wang J
R960-25 was used in Immunoprecipitation to demonstrate a TET1-PSPC1-Neat1 molecular axis that modulates PRC2-binding affinity to chromatin and bivalent gene transcripts in controlling stem cell bivalency.
Tue Jun 07 00:00:00 UTC 2022
St18 specifies globus pallidus projection neuron identity in MGE lineage.
Nature communications
Nunnelly LF,Campbell M,Lee DI,Dummer P,Gu G,Menon V,Au E
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, Immunohistochemistry to identify St18 as a key regulator of projection neuron vs. interneuron identity.
Wed Dec 14 00:00:00 UTC 2022
Transmembrane serine protease TMPRSS2 implicated in SARS-CoV-2 infection is autoactivated intracellularly and requires N-glycosylation for regulation.
The Journal of biological chemistry
Zhang Y,Sun S,Du C,Hu K,Zhang C,Liu M,Wu Q,Dong N
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry to provide new insights into the cellular mechanism in regulating TMPRSS2 biosynthesis and function.
Thu Dec 01 00:00:00 UTC 2022
Transmembrane serine protease TMPRSS2 implicated in SARS-CoV-2 infection is autoactivated intracellularly and requires N-glycosylation for regulation.
The Journal of biological chemistry
Zhang Y,Sun S,Du C,Hu K,Zhang C,Liu M,Wu Q,Dong N
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry to provide new insights into the cellular mechanism in regulating TMPRSS2 biosynthesis and function.
Thu Dec 01 00:00:00 UTC 2022
Multiphase coalescence mediates Hippo pathway activation.
Cell
Wang L,Choi K,Su T,Li B,Wu X,Zhang R,Driskill JH,Li H,Lei H,Guo P,Chen EH,Zheng Y,Pan D
R960-25 was used in Immunocytochemistry to show that upstream regulators of the Hippo signaling pathway form functionally antagonizing condensates, and their coalescence into a common phase provides a mode of counteracting the function of biomolecular condensates without condensate dissolution.
Thu Nov 10 00:00:00 UTC 2022
Isolation of Single-Domain Antibodies to Transmembrane Proteins Using Magnetized Yeast Cell Targets.
Methods in molecular biology (Clifton, N.J.)
Bacon K,Menegatti S,Rao BM
R960-25 was used in In vitro experiments to discuss how to assess the affinity and specificity of the isolated nanobodies.
Fri Feb 18 00:00:00 UTC 2022
Mapping R-Loops Using Catalytically Inactive RNaseH1 (R-ChIP).
Methods in molecular biology (Clifton, N.J.)
Zhang X,Hao Y,Fu XD
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, ChIP Assay to provide a n overview on R-loop mapping strategies along with a detailed protocol based on the use of catalytically inactive RNaseH1, an evolutionarily conserved protein responsible for R-loop recognition and resolution.
Fri Jun 17 00:00:00 UTC 2022
ALS-associated KIF5A mutations abolish autoinhibition resulting in a toxic gain of function.
Cell reports
Baron DM,Fenton AR,Saez-Atienzar S,Giampetruzzi A,Sreeram A,Keagle PJ,Doocy VR,Smith NJ,Danielson EW,Andresano M,McCormack MC,Garcia J,Bercier V,Van Den Bosch L,Brent JR,Fallini C,Traynor BJ,Holzbaur ELF,Landers JE
R960-25 was used in Simple Western - Size to support the hypothesis that causative ALS mutations result in a toxic gain of function in the intracellular motor KIF5A that disrupts intracellular trafficking and neuronal homeostasis.
Tue Apr 05 00:00:00 UTC 2022
Malaria parasites utilize two essential plasma membrane fusogens for gamete fertilization.
Cellular and molecular life sciences : CMLS
Kumar S,Valansi C,Haile MT,Li X,Flyak K,Dwivedy A,Abatiyow BA,Leeb AS,Kennedy SY,Camargo NM,Vaughan AM,Brukman NG,Podbilewicz B,Kappe SHI
R960-25 was used in Immunocytochemistry-immunofluorescence to establish nonredundant essential roles for PfHAP2 and PfHAP2p in mediating gamete fusion in Plasmodium.
Fri Oct 14 00:00:00 UTC 2022
Fluorizoline Blocks the Interaction between Prohibitin-2 and γ-Glutamylcyclotransferase and Induces Expression in MCF7 Breast Cancer Cells.
Molecular pharmacology
Takagi H,Moyama C,Taniguchi K,Ando K,Matsuda R,Ando S,Ii H,Kageyama S,Kawauchi A,Chouha N,Désaubry L,Nakata S
R960-25 was used in Western Blotting to show that fluorizoline may be a promising novel anticancer drug candidate that induces p21 expression and blocks cell-cycle progression in human cancer cell lines.
Tue Feb 01 00:00:00 UTC 2022
The HIV-1 protein Nef activates the Tec family kinase Btk by stabilizing an intermolecular SH3-SH2 domain interaction.
Science signaling
Aryal M,Lin D,Regan K,Du S,Shi H,Alvarado JJ,Ilina TV,Andreotti AH,Smithgall TE
R960-25 was used in Immunocytochemistry-immunofluorescence to reveal that the interaction with Nef stabilizes Btk dimers through the SH3-SH2 interface to promote kinase activity and show that the HIV-1 Nef protein evolved distinct mechanisms to activate Src and Tec family tyrosine kinases to enhance viral replication.
Tue Sep 20 00:00:00 UTC 2022
The non-adrenergic imidazoline-1 receptor protein nischarin is a key regulator of astrocyte glutamate uptake.
iScience
Gupta S,Bazargani N,Drew J,Howden JH,Modi S,Al Awabdh S,Marie H,Attwell D,Kittler JT
R960-25 was used in Western Blotting to study the surface expression and function of astrocytic GLT-1 to show it can be rapidly modulated through the interaction of its N-terminus with the nonadrenergic imidazoline-1 receptor protein, Nischarin, providing molecular insight into the regulation of GLT-1 in neurological disorders.
Fri Apr 15 00:00:00 UTC 2022
Immunogenetics associated with severe coccidioidomycosis.
JCI insight
Hsu AP,Korzeniowska A,Aguilar CC,Gu J,Karlins E,Oler AJ,Chen G,Reynoso GV,Davis J,Chaput A,Peng T,Sun L,Lack JB,Bays DJ,Stewart ER,Waldman SE,Powell DA,Donovan FM,Desai JV,Pouladi N,Long Priel DA,Yamanaka D,Rosenzweig SD,Niemela JE,Stoddard J,Freeman AF,Zerbe CS,Kuhns DB,Lussier YA,Olivier KN,Boucher RC,Hickman HD,Frelinger J,Fierer J,Shubitz LF,Leto TL,Thompson GR,Galgiani JN,Lionakis MS,Holland SM
R960-25 was used in Western Blotting to find that early defects in innate fungal recognition and response are critical to the host control of the pathogen Coccidioides; defects in these pathways contribute to the development of severe coccidioidomycosis, a predisposition only apparent in the corresponding locale.
Tue Nov 22 00:00:00 UTC 2022
HP1 maintains protein stability of H3K9 methyltransferases and demethylases.
EMBO reports
Maeda R,Tachibana M
R960-25 was used in Western Blotting to indicate that mammalian HP1 forms a heterochromatin hub that governs protein stability of H3K9 MTs and H3K9 DMs.
Tue Apr 05 00:00:00 UTC 2022
Tumor elimination by clustered microRNAs miR-306 and miR-79 via noncanonical activation of JNK signaling.
eLife
Wang Z,Xia X,Li J,Igaki T
R960-25 was used in Western Blotting to show that, while JNK activity is essential for tumor growth, elevation of miR-306 or miR-79 overactivate JNK signaling to the lethal level via noncanonical JNK pathway and thus eliminate tumors, providing a new miRNA-based strategy against cancer.
Wed Oct 12 00:00:00 UTC 2022
Proteomic profiling in cerebral amyloid angiopathy reveals an overlap with CADASIL highlighting accumulation of HTRA1 and its substrates.
Acta neuropathologica communications
Zellner A,Müller SA,Lindner B,Beaufort N,Rozemuller AJM,Arzberger T,Gassen NC,Lichtenthaler SF,Kuster B,Haffner C,Dichgans M
R960-25 was used in Western Blot to investigate the role of HTRA1-dependent protein homeostasis in the cerebral amyloid angiopathy microvasculature and a molecular connection between multiple types of brain microvascular disease.
Mon Jan 24 00:00:00 UTC 2022
A circadian clock translational control mechanism targets specific mRNAs to cytoplasmic messenger ribonucleoprotein granules.
Cell reports
Castillo KD,Wu C,Ding Z,Lopez-Garcia OK,Rowlinson E,Sachs MS,Bell-Pedersen D
R960-25 was used in Western Blot to reveal that the clock regulates rhythmic translation of specific mRNAs through rhythmic eIF2α activity and cmRNPg metabolism.
Tue Dec 27 00:00:00 UTC 2022
Chronic Kidney Disease Is Associated With Increased Cardiac Corin Expression But Decreased Proatrial Natriuretic Peptide Conversion Activity.
Journal of the American Heart Association
Yang SF,Li SY,Lin FY,Hsieh TH,Huang PH,Lin SJ
R960-25 was used in Western Blotting to investigate if corin dysfunction may play a significant role in the pathogenesis of Chronic kidney disease-associated cardiomyopathy.
Tue Jul 19 00:00:00 UTC 2022
Deubiquitinase ubiquitin-specific protease 3 (USP3) inhibits HIV-1 replication via promoting APOBEC3G (A3G) expression in both enzyme activity-dependent and -independent manners.
Chinese medical journal
Zhao S,Zheng B,Wang L,Cui W,Jiang C,Li Z,Gao W,Zhang W
R960-25 was used in ChIP assay to demonstrate that USP3 specifically inhibits HIV-1 replication in an A3G-dependent manner.
Sun Nov 20 00:00:00 UTC 2022
TRK-fused gene (TFG) regulates ULK1 stability via TRAF3-mediated ubiquitination and protects macrophages from LPS-induced pyroptosis.
Cell death & disease
Shi JH,Ling C,Wang TT,Zhang LN,Liu WW,Qin Y,Tan YH,Cui NP,Ni ZY
R960-25 was used in Immunoprecipitation to determine the function of TRK-fused genes in LPS-induced inflammation and pyroptotic cell death.
Fri Jan 28 00:00:00 UTC 2022
PPM1D mutations are oncogenic drivers of de novo diffuse midline glioma formation.
Nature communications
Khadka P,Reitman ZJ,Lu S,Buchan G,Gionet G,Dubois F,Carvalho DM,Shih J,Zhang S,Greenwald NF,Zack T,Shapira O,Pelton K,Hartley R,Bear H,Georgis Y,Jarmale S,Melanson R,Bonanno K,Schoolcraft K,Miller PG,Condurat AL,Gonzalez EM,Qian K,Morin E,Langhnoja J,Lupien LE,Rendo V,Digiacomo J,Wang D,Zhou K,Kumbhani R,Guerra Garcia ME,Sinai CE,Becker S,Schneider R,Vogelzang J,Krug K,Goodale A,Abid T,Kalani Z,Piccioni F,Beroukhim R,Persky NS,Root DE,Carcaboso AM,Ebert BL,Fuller C,Babur O,Kieran MW,Jones C,Keshishian H,Ligon KL,Carr SA,Phoenix TN,Bandopadhayay P
R960-25 was used in Western Blot to analyze whole genome sequences of 170 pediatric high-grade gliomas and find that truncating mutations in PPM1D that increase the stability of its phosphatase are clonal driver events in 11% of Diffuse Midline Gliomas and are enriched in primary pontine tumors.
Tue Feb 01 00:00:00 UTC 2022
A yeast suppressor screen links Coa4 to the mitochondrial copper delivery pathway for cytochrome c oxidase.
Genetics
Swaminathan AB,Soma S,Vicary AC,Zulkifli M,Kaur H,Gohil VM
R960-25 was used in Western Blotting to provide genetic evidences for the role of Coa4 in the copper delivery pathway to the CuB site of cytochrome c oxidase.
Sat Jul 30 00:00:00 UTC 2022
Regulation of mitophagy by the NSL complex underlies genetic risk for Parkinson's disease at 16q11.2 and MAPT H1 loci.
Brain : a journal of neurology
Soutar MPM,Melandri D,O'Callaghan B,Annuario E,Monaghan AE,Welsh NJ,D'Sa K,Guelfi S,Zhang D,Pittman A,Trabzuni D,Verboven AHA,Pan KS,Kia DA,Bictash M,Gandhi S,Houlden H,Cookson MR,Kasri NN,Wood NW,Singleton AB,Hardy J,Whiting PJ,Blauwendraat C,Whitworth AJ,Manzoni C,Ryten M,Lewis PA,Plun-Favreau H
R960-25 was used in Western Blotting to suggest PINK1-mitophagy is a contributing factor to idiopathic Parkinson's disease.
Mon Dec 19 00:00:00 UTC 2022
MicroRNA-7 regulates melanocortin circuits involved in mammalian energy homeostasis.
Nature communications
LaPierre MP,Lawler K,Godbersen S,Farooqi IS,Stoffel M
R960-25 was used in Immunohistochemistry to demonstrate that miR-7 suppresses gene networks involved in the hypothalamic melanocortin pathway to regulate mammalian energy homeostasis.
Thu Sep 29 00:00:00 UTC 2022
Mislocalization of protein kinase A drives pathology in Cushing's syndrome.
Cell reports
Omar MH,Byrne DP,Jones KN,Lakey TM,Collins KB,Lee KS,Daly LA,Forbush KA,Lau HT,Golkowski M,McKnight GS,Breault DT,Lefrançois-Martinez AM,Martinez A,Eyers CE,Baird GS,Ong SE,Smith FD,Eyers PA,Scott JD
R960-25 was used in Immunoprecipitation, Immunocytochemistry to conclude that aberrant spatiotemporal regulation of each Cushing's variant promotes the transmission of distinct downstream pathogenic signals.
Tue Jul 12 00:00:00 UTC 2022
Mislocalization of protein kinase A drives pathology in Cushing's syndrome.
Cell reports
Omar MH,Byrne DP,Jones KN,Lakey TM,Collins KB,Lee KS,Daly LA,Forbush KA,Lau HT,Golkowski M,McKnight GS,Breault DT,Lefrançois-Martinez AM,Martinez A,Eyers CE,Baird GS,Ong SE,Smith FD,Eyers PA,Scott JD
R960-25 was used in Immunoprecipitation, Immunocytochemistry to conclude that aberrant spatiotemporal regulation of each Cushing's variant promotes the transmission of distinct downstream pathogenic signals.
Tue Jul 12 00:00:00 UTC 2022
Monoallelic CRMP1 gene variants cause neurodevelopmental disorder.
eLife
Ravindran E,Arashiki N,Becker LL,Takizawa K,Lévy J,Rambaud T,Makridis KL,Goshima Y,Li N,Vreeburg M,Demeer B,Dickmanns A,Stegmann APA,Hu H,Nakamura F,Kaindl AM
R960-25 was used in Immunocytochemistry to report for the first-time variants in the CRMP1 gene and emphasize its key role in brain development and function by linking directly to a human neurodevelopmental disease.
Tue Dec 13 00:00:00 UTC 2022
Temporal and thermal profiling of the Toxoplasma proteome implicates parasite Protein Phosphatase 1 in the regulation of Ca2+-responsive pathways.
eLife
Herneisen AL,Li ZH,Chan AW,Moreno SNJ,Lourido S
R960-25 was used in Immunocytochemistry and Western Blotting to characterize protein phosphatase 1 (PP1) as a Ca2+-responsive enzyme that relocalized to the parasite apex upon Ca2+ store release.
Wed Aug 17 00:00:00 UTC 2022
Temporal and thermal profiling of the Toxoplasma proteome implicates parasite Protein Phosphatase 1 in the regulation of Ca2+-responsive pathways.
eLife
Herneisen AL,Li ZH,Chan AW,Moreno SNJ,Lourido S
R960-25 was used in Immunocytochemistry and Western Blotting to characterize protein phosphatase 1 (PP1) as a Ca2+-responsive enzyme that relocalized to the parasite apex upon Ca2+ store release.
Wed Aug 17 00:00:00 UTC 2022
Single-cell profiling of transcriptome and histone modifications with EpiDamID.
Molecular cell
Rang FJ,de Luca KL,de Vries SS,Valdes-Quezada C,Boele E,Nguyen PD,Guerreiro I,Sato Y,Kimura H,Bakkers J,Kind J
R960-25 was used in Western Blotting to conclude that EpiDamID is a new addition to a vast toolbox to study chromatin states during dynamic cellular processes.
Thu May 19 00:00:00 UTC 2022
A PKA inhibitor motif within SMOOTHENED controls Hedgehog signal transduction.
Nature structural & molecular biology
Happ JT,Arveseth CD,Bruystens J,Bertinetti D,Nelson IB,Olivieri C,Zhang J,Hedeen DS,Zhu JF,Capener JL,Bröckel JW,Vu L,King CC,Ruiz-Perez VL,Ge X,Veglia G,Herberg FW,Taylor SS,Myers BR
R960-25 was used in Immunocytochemistry-immunofluorescence to define a mode of GPCR-PKA communication that may be harnessed by a range of membrane receptors and kinases.
Sat Oct 01 00:00:00 UTC 2022
Stress-induced phase separation of ERES components into Sec bodies precedes ER exit inhibition in mammalian cells.
Journal of cell science
van Leeuwen W,Nguyen DTM,Grond R,Veenendaal T,Rabouille C,Farías GG
R960-25 was used in Immunocytochemistry to illustrate that ERES and Sec body assembly, driven by Sec16A, regulates protein exit from ER during and upon stress relief in mammalian cells, thus providing a conserved pro-survival mechanism in response to stress.
Thu Dec 01 00:00:00 UTC 2022
Structural and biophysical characterization of transcription factor HNF-1A as a tool to study MODY3 diabetes variants.
The Journal of biological chemistry
Kind L,Raasakka A,Molnes J,Aukrust I,Bjørkhaug L,Njølstad PR,Kursula P,Arnesen T
R960-25 was used in Western Blot to present a novel approach for the dissection of structurally unstable and DNA binding defective variants. This study indicates that structural and biochemical investigation of HNF-1A is a valuable tool in reliable variant classification needed for precision diabetes diagnostics and management.
Fri Apr 01 00:00:00 UTC 2022
A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs.
The Journal of biological chemistry
Haugen RJ,Arvola RM,Connacher RP,Roden RT,Goldstrohm AC
R960-25 was used in Western Blotting to identify the motifs that are necessary and sufficient for the activity of the third repression domain of Pumilio, designated RD3, which is present in all isoforms and has conserved regulatory function.
Thu Sep 01 00:00:00 UTC 2022
Human transcription factor protein interaction networks.
Nature communications
Göös H,Kinnunen M,Salokas K,Tan Z,Liu X,Yadav L,Zhang Q,Wei GH,Varjosalo M
R960-25 was used in Dot blot to systemically characterize the protein-protein interactions of a selected set of 109 human transcription factors.
Wed Feb 09 00:00:00 UTC 2022
SETDB1 acts as a topological accessory to Cohesin via an H3K9me3-independent, genomic shunt for regulating cell fates.
Nucleic acids research
Warrier T,El Farran C,Zeng Y,Ho BSQ,Bao Q,Zheng ZH,Bi X,Ng HH,Ong DST,Chu JJH,Sanyal A,Fullwood MJ,Collins JJ,Li H,Xu J,Loh YH
R960-25 was used in Western Blotting to investigate the regulatory role of SETDB1 beyond its known function in depositing the repressive H3K9me3 mark.
Fri Jul 22 00:00:00 UTC 2022
An expansion of the non-coding genome and its regulatory potential underlies vertebrate neuronal diversity.
Neuron
Closser M,Guo Y,Wang P,Patel T,Jang S,Hammelman J,De Nooij JC,Kopunova R,Mazzoni EO,Ruan Y,Gifford DK,Wichterle H
R960-25 was used in Immunocytochemistry to show that neuronal genes are associated with highly complex gene regulatory systems composed of independent cell-type- and cell-stage-specific regulatory elements that reside in expanded non-coding genomic domains.
Wed Jan 05 00:00:00 UTC 2022
Cdk8 attenuates lipogenesis by inhibiting SREBP-dependent transcription in Drosophila.
Disease models & mechanisms
Li X,Zhang M,Liu M,Liu TH,Hemba-Waduge RU,Ji JY
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, Immunohistochemistry to support that the concerted actions of Cdk8 and MED15 are essential for the tight regulation of SREBP-dependent transcription.
Tue Nov 01 00:00:00 UTC 2022
Serine 13 of the human cytomegalovirus viral cyclin-dependent kinase UL97 is required for regulatory protein 14-3-3 binding and UL97 stability.
The Journal of biological chemistry
Iwahori S,Umaña AC,Kalejta RF,Murata T
R960-25 was used in Western Blotting to conclude that 14-3-3 proteins interact with and stabilize UL97 during HCMV infection.
Tue Nov 01 00:00:00 UTC 2022
ER-mitochondrial contact protein Miga regulates autophagy through Atg14 and Uvrag.
Cell reports
Xu L,Qiu Y,Wang X,Shang W,Bai J,Shi K,Liu H,Liu JP,Wang L,Tong C
R960-25 was used in Immunoprecipitation to identify a mitochondrial protein that regulates autophagy by recruiting two alternative components of the PI3K complex present at the endoplasmic reticulum-mitochondrial contact sites.
Tue Nov 01 00:00:00 UTC 2022
ER-mitochondrial contact protein Miga regulates autophagy through Atg14 and Uvrag.
Cell reports
Xu L,Qiu Y,Wang X,Shang W,Bai J,Shi K,Liu H,Liu JP,Wang L,Tong C
R960-25 was used in Immunoprecipitation to identify a mitochondrial protein that regulates autophagy by recruiting two alternative components of the PI3K complex present at the endoplasmic reticulum-mitochondrial contact sites.
Tue Nov 01 00:00:00 UTC 2022
Crosstalk between CRISPR-Cas9 and the human transcriptome.
Nature communications
Smargon AA,Madrigal AA,Yee BA,Dong KD,Mueller JR,Yeo GW
R960-25 was used in Immunocytochemistry to study through eCLIP the interactions between Cas9 and human RNA.
Wed Mar 02 00:00:00 UTC 2022
The Oligomeric Assemblies of Cytomegalovirus Core Nuclear Egress Proteins Are Associated with Host Kinases and Show Sensitivity to Antiviral Kinase Inhibitors.
Viruses
Kicuntod J,Häge S,Hahn F,Sticht H,Marschall M
R960-25 was used in PCR to underline the functional importance of the oligomerization of the HCMV-specific NEC that is both phosphorylation-dependent and sensitive to antiviral kinase inhibitors.
Wed May 11 00:00:00 UTC 2022
S146L in MYC is a context-dependent activating substitution in cancer development.
PloS one
Hinds JW,Feris EJ,Wilkins OM,Deary LT,Wang X,Cole MD
R960-25 was used in Proximity Ligation Assay to show that this mutation enhances the interaction between MYC and its cofactor TRRAP and may enhance oncogenic MYC activity in certain cellular contexts.
Tue Aug 30 00:00:00 UTC 2022
Transient regulation of focal adhesion via Tensin3 is required for nascent oligodendrocyte differentiation.
eLife
Merour E,Hmidan H,Marie C,Helou PH,Lu H,Potel A,Hure JB,Clavairoly A,Shih YP,Goudarzi S,Dussaud S,Ravassard P,Hafizi S,Lo SH,Hassan BA,Parras C
R960-25 was used in Western Blot to reveal a specific time window during which transcriptional upregulation of Tns3 in immature OLs is required for OL differentiation likely by mediating integrin-β1 survival signaling to the actin cytoskeleton as OL undergo the large morphological changes required for their terminal differentiation.
Mon Oct 10 00:00:00 UTC 2022
SARS-CoV-2 ORF7a potently inhibits the antiviral effect of the host factor SERINC5.
Nature communications
Timilsina U,Umthong S,Ivey EB,Waxman B,Stavrou S
R960-25 was used in Immunoprecipitation to show that SERINC5 inhibits SARS-CoV-2 entry by blocking virus-cell fusion, and SARS-CoV-2 ORF7a counteracts the antiviral effect of SERINC5 by blocking the incorporation of over expressed SERINC5 in budding virions.
Thu May 26 00:00:00 UTC 2022
SARS-CoV-2 ORF7a potently inhibits the antiviral effect of the host factor SERINC5.
Nature communications
Timilsina U,Umthong S,Ivey EB,Waxman B,Stavrou S
R960-25 was used in Immunoprecipitation to show that SERINC5 inhibits SARS-CoV-2 entry by blocking virus-cell fusion, and SARS-CoV-2 ORF7a counteracts the antiviral effect of SERINC5 by blocking the incorporation of over expressed SERINC5 in budding virions.
Thu May 26 00:00:00 UTC 2022
IRF3 inhibits nuclear translocation of NF-κB to prevent viral inflammation.
Proceedings of the National Academy of Sciences of the United States of America
Popli S,Chakravarty S,Fan S,Glanz A,Aras S,Nagy LE,Sen GC,Chakravarti R,Chattopadhyay S
R960-25 was used in Proximity Ligation Assay (PLA) to demonstrate IRF3 deployed a three-pronged attack on virus replication and the accompanying inflammation.
Tue Sep 13 00:00:00 UTC 2022
Foxa2 and Pet1 Direct and Indirect Synergy Drive Serotonergic Neuronal Differentiation.
Frontiers in neuroscience
Aydin B,Sierk M,Moreno-Estelles M,Tejavibulya L,Kumar N,Flames N,Mahony S,Mazzoni EO
R960-25 was used in Immunocytochemistry-immunofluorescence to highlight the challenges in formulating generalizable rules for describing the behavior of TF combinations that program distinct neuronal subtypes.
Tue Aug 30 00:00:00 UTC 2022
SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.
Nucleic acids research
Guan Y,Lin H,Leu NA,Ruthel G,Fuchs SY,Busino L,Luo M,Wang PJ
R960-25 was used in Western Blotting to demonstrate that SCF ubiquitin E3 ligase prevents hyperactive DSB formation through proteasome-mediated degradation of HORMAD1 and subsequent modulation of the pre-DSB complex during meiosis.
Fri May 20 00:00:00 UTC 2022
Studying Cell Polarity Dynamics During Cancer Initiation Using Inducible 3D Organotypic Cultures.
Methods in molecular biology (Clifton, N.J.)
Catterall R,Kurdieh R,McCaffrey L
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot to establish 3D organoids from human cell lines or mouse primary cells with inducible oncogene expression in polarized epithelial structures to investigate mechanisms of tumor initiation, luminal filling, and growth.
Fri Feb 18 00:00:00 UTC 2022
Female meiosis II and pronuclear fusion require the microtubule transport factor Bicaudal D.
Development (Cambridge, England)
Vazquez-Pianzola P,Beuchle D,Saro G,Hernández G,Maldonado G,Brunßen D,Meister P,Suter B
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, Immunohistochemistry to find that reducing the activity of BicD specifically in freshly laid Drosophila eggs by acute protein degradation requires BicD to produce normal female meiosis II products.
Fri Jul 01 00:00:00 UTC 2022
The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.
iScience
Zou L,Moch C,Graille M,Chapat C
R960-25 was used in Western Blotting to reveal the potential impact of NSP2 on the post-transcriptional silencing of gene expression in human cells, pointing out 4EHP-GIGYF2 targeting as a possible strategy of SARS-CoV-2 to take over the silencing machinery and to suppress host defenses.
Fri Jul 15 00:00:00 UTC 2022
MYPT1-PP1β phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis.
Nature communications
Takahashi H,Yang G,Yoneshiro T,Abe Y,Ito R,Yang C,Nakazono J,Okamoto-Katsuyama M,Uchida A,Arai M,Jin H,Choi H,Tumenjargal M,Xie S,Zhang J,Sagae H,Zhao Y,Yamaguchi R,Nomura Y,Shimizu Y,Yamada K,Yasuda S,Kimura H,Tanaka T,Wada Y,Kodama T,Aburatani H,Zhu MS,Inagaki T,Osborne TF,Kawamura T,Ishihama Y,Matsumura Y,Sakai J
R960-25 was used in Western Blotting to uncover regulatory cross-talk involved in beige adipogenesis that coordinates epigenetic regulation with direct activation of the mechano-sensitive YAP/TAZ transcriptional co-activators.
Thu Sep 29 00:00:00 UTC 2022
Pharmacological chaperone-rescued cystic fibrosis CFTR-F508del mutant overcomes PRAF2-gated access to endoplasmic reticulum exit sites.
Cellular and molecular life sciences : CMLS
Saha K,Chevalier B,Doly S,Baatallah N,Guilbert T,Pranke I,Scott MGH,Enslen H,Guerrera C,Chuon C,Edelman A,Sermet-Gaudelus I,Hinzpeter A,Marullo S
R960-25 was used in Immunoprecipitation to demonstrate that some pharmacological chaperones that efficiently rescue CFTR-F508del loss of function in CF patients target CFTR-F508del retention by PRAF2 operating with various mechanisms.
Tue Sep 27 00:00:00 UTC 2022
A light-gated transcriptional recorder for detecting cell-cell contacts.
eLife
Cho KF,Gillespie SM,Kalogriopoulos NA,Quezada MA,Jacko M,Monje M,Ting AY
R960-25 was used in Western Blotting to show that TRACC detects cell-cell contacts with high specificity and sensitivity in mammalian cell culture and that it can be used to interrogate interactions between neurons and glioma, a form of brain cancer.
Mon Mar 21 00:00:00 UTC 2022
MicroID2: A Novel Biotin Ligase Enables Rapid Proximity-Dependent Proteomics.
Molecular & cellular proteomics : MCP
Johnson BS,Chafin L,Farkas D,Adair J,Elhance A,Farkas L,Bednash JS,Londino JD
R960-25 was used in Western Blotting to develop and demonstrate the utility of MicroID2 in mass spectrometry experiments by localizing MicroID2 constructs to subcellular organelles and measuring proximal interactions.
Fri Jul 01 00:00:00 UTC 2022
Anatomic position determines oncogenic specificity in melanoma.
Nature
Weiss JM,Hunter MV,Cruz NM,Baggiolini A,Tagore M,Ma Y,Misale S,Marasco M,Simon-Vermot T,Campbell NR,Newell F,Wilmott JS,Johansson PA,Thompson JF,Long GV,Pearson JV,Mann GJ,Scolyer RA,Waddell N,Montal ED,Huang TH,Jonsson P,Donoghue MTA,Harris CC,Taylor BS,Xu T,Chaligné R,Shliaha PV,Hendrickson R,Jungbluth AA,Lezcano C,Koche R,Studer L,Ariyan CE,Solit DB,Wolchok JD,Merghoub T,Rosen N,Hayward NK,White RM
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, Immunohistochemistry to suggest that the anatomic position of the cell of origin endows it with a unique transcriptional state that makes it susceptible to only certain oncogenic insults.
Fri Apr 01 00:00:00 UTC 2022
Phosphosites of the yeast centrosome component Spc110 contribute to cell cycle progression and mitotic exit.
Biology open
Abbasi M,Julner A,Lim YT,Zhao T,Sobota RM,Menéndez-Benito V
R960-25 was used in Western Blot to propose that Spc110 phosphorylation at S11 and S36 is required to regulate timely cell cycle progression in budding yeast.
Tue Nov 01 00:00:00 UTC 2022
Distinguishing between recruitment and spread of silent chromatin structures in Saccharomyces cerevisiae.
eLife
Brothers M,Rine J
R960-25 was used in Immunoprecipitation, Western Blot to track the positions first methylated after induction and found that repression of genes at HML and HMR began before Sir3 occupied the entire locus.
Mon Jan 24 00:00:00 UTC 2022
Distinguishing between recruitment and spread of silent chromatin structures in Saccharomyces cerevisiae.
eLife
Brothers M,Rine J
R960-25 was used in Immunoprecipitation, Western Blot to track the positions first methylated after induction and found that repression of genes at HML and HMR began before Sir3 occupied the entire locus.
Mon Jan 24 00:00:00 UTC 2022
ML216 Prevents DNA Damage-Induced Senescence by Modulating DBC1-BLM Interaction.
Cells
Cui F,Han X,Zhang X,Wang S,Liang N,Tan Q,Sha W,Li J
R960-25 was used in Immunoprecipitation to reveal a unique mechanism preventing DNA-damaged cells from becoming senescent, which may be regulated by the use of ML216 as a potential treatment for senescence-related diseases.
Thu Dec 29 00:00:00 UTC 2022
A putative cap binding protein and the methyl phosphate capping enzyme Bin3/MePCE function in telomerase biogenesis.
Nature communications
Páez-Moscoso DJ,Ho DV,Pan L,Hildebrand K,Jensen KL,Levy MJ,Florens L,Baumann P
R960-25 was used in Immunoprecipitation to assess whether Pof8, a member of the LARP7 protein family and a constitutive component of telomerase in fission yeast, has binding partners in addition to known telomerase components.
Fri Feb 25 00:00:00 UTC 2022
EMC is required for biogenesis of Xport-A, an essential chaperone of Rhodopsin-1 and the TRP channel.
EMBO reports
Gaspar CJ,Vieira LC,Santos CC,Christianson JC,Jakubec D,Strisovsky K,Adrain C,Domingos PM
R960-25 was used in Western Blotting to reveal a role for Xport-A in a glycosylation-dependent triage mechanism during rhodopsin-1 biogenesis in the endoplasmic reticulum.
Wed Jan 05 00:00:00 UTC 2022
Differential cofactor dependencies define distinct types of human enhancers.
Nature
Neumayr C,Haberle V,Serebreni L,Karner K,Hendy O,Boija A,Henninger JE,Li CH,Stejskal K,Lin G,Bergauer K,Pagani M,Rath M,Mechtler K,Arnold CD,Stark A
R960-25 was used in Western Blotting to categorize human enhancers by their cofactor dependencies and show that these categories provide a framework to understand the sequence and chromatin diversity of enhancers and their roles in different gene-regulatory programmes.
Wed Jun 01 00:00:00 UTC 2022
EV71 3C protease cleaves host anti-viral factor OAS3 and enhances virus replication.
Virologica Sinica
Zhou X,Tian L,Wang J,Zheng B,Zhang W
R960-25 was used in Western Blotting to demonstrate that EV71 utilizes autologous 3C protease to cleave intracellular OAS3 and enhance viral replication.
Wed Jun 01 00:00:00 UTC 2022
Loss of phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) in mesenchymal stem cells leads to osteopenia by impairing bone remodeling.
The Journal of biological chemistry
Yan Q,Gao H,Yao Q,Ling K,Xiao G
R960-25 was used in Western Blot to determine whether phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) plays a role in skeletal development and homeostasis and what the underlying mechanisms are.
Tue Mar 01 00:00:00 UTC 2022
In vivo characterization of Drosophila golgins reveals redundancy and plasticity of vesicle capture at the Golgi apparatus.
Current biology : CB
Park SY,Muschalik N,Chadwick J,Munro S
R960-25 was used in Immunohistochemistry to show that ectopic forms can capture intra-Golgi transport vesicles, but strikingly, the cargo present in the vesicles captured by each golgin varies between tissues.
Mon Nov 07 00:00:00 UTC 2022
Autosomal recessive congenital ichthyosis caused by a pathogenic missense variant in CLDN1.
American journal of medical genetics. Part A
Mohamad J,Samuelov L,Assaf S,Malki L,Malovitski K,Meijers O,Adir N,Granot E,Pavlovsky M,Sarig O,Sprecher E
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot to describe the first pathogenic missense variant in CLDN1 shown to result in autosomal recessive congenital ichthyosis.
Sat Oct 01 00:00:00 UTC 2022
Regulation of PD-L1 through direct binding of cholesterol to CRAC motifs.
Science advances
Wang Q,Cao Y,Shen L,Xiao T,Cao R,Wei S,Tang M,Du L,Wu H,Wu B,Yu Y,Wang S,Wen M,OuYang B
R960-25 was used in Western Blotting to demonstrate that cholesterol can directly bind to the transmembrane domain of PD-L1 through two cholesterol-recognition amino acid consensus (CRAC) motifs, forming a sandwich-like architecture and stabilizing PD-L1 to prevent downstream degradation.
Fri Aug 26 00:00:00 UTC 2022
Engineered Wnt ligands enable blood-brain barrier repair in neurological disorders.
Science (New York, N.Y.)
Martin M,Vermeiren S,Bostaille N,Eubelen M,Spitzer D,Vermeersch M,Profaci CP,Pozuelo E,Toussay X,Raman-Nair J,Tebabi P,America M,De Groote A,Sanderson LE,Cabochette P,Germano RFV,Torres D,Boutry S,de Kerchove d'Exaerde A,Bellefroid EJ,Phoenix TN,Devraj K,Lacoste B,Daneman R,Liebner S,Vanhollebeke B
R960-25 was used in Dot Blot to reveal that the signaling specificity of Wnt ligands is adjustable and defines a modality to treat CNS disorders by normalizing the BBB.
Fri Feb 18 00:00:00 UTC 2022
Engineered Wnt ligands enable blood-brain barrier repair in neurological disorders.
Science (New York, N.Y.)
Martin M,Vermeiren S,Bostaille N,Eubelen M,Spitzer D,Vermeersch M,Profaci CP,Pozuelo E,Toussay X,Raman-Nair J,Tebabi P,America M,De Groote A,Sanderson LE,Cabochette P,Germano RFV,Torres D,Boutry S,de Kerchove d'Exaerde A,Bellefroid EJ,Phoenix TN,Devraj K,Lacoste B,Daneman R,Liebner S,Vanhollebeke B
R960-25 was used in Dot Blot to reveal that the signaling specificity of Wnt ligands is adjustable and defines a modality to treat CNS disorders by normalizing the BBB.
Fri Feb 18 00:00:00 UTC 2022
Development of an HIV reporter virus that identifies latently infected CD4+ T cells.
Cell reports methods
Kim EH,Manganaro L,Schotsaert M,Brown BD,Mulder LCF,Simon V
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry to show that this viral vector permits the interrogation of HIV latency at single-cell resolution, revealing mechanisms of latency establishment and allowing the characterization of effective latency-reversing agents.
Mon Jun 20 00:00:00 UTC 2022
ER-stress promotes VHL-independent degradation of hypoxia-inducible factors via FBXW1A/βTrCP.
Redox biology
Mennerich D,Kubaichuk K,Raza GS,Fuhrmann DC,Herzig KH,Brüne B,Kietzmann T
R960-25 was used in Western Blotting to discover previously unrecognized links between the HIF pathway and the ER stress response and uncovered an essential survival pathway for cells under ER stress.
Fri Apr 01 00:00:00 UTC 2022
Identification of the stress granule transcriptome via RNA-editing in single cells and in vivo.
Cell reports methods
van Leeuwen W,VanInsberghe M,Battich N,Salmén F,van Oudenaarden A,Rabouille C
R960-25 was used in Sample Preparation to use TRIBE (target of RNA-binding proteins identified by editing) to profile stress granule RNAs.
Mon Jun 20 00:00:00 UTC 2022
Genomic and phenotypic characterization of 404 individuals with neurodevelopmental disorders caused by CTNNB1 variants.
Genetics in medicine : official journal of the American College of Medical Genetics
Kayumi S,Pérez-Jurado LA,Palomares M,Rangu S,Sheppard SE,Chung WK,Kruer MC,Kharbanda M,Amor DJ,McGillivray G,Cohen JS,García-Miñaúr S,van Eyk CL,Harper K,Jolly LA,Webber DL,Barnett CP,Santos-Simarro F,Pacio-Míguez M,Pozo AD,Bakhtiari S,Deardorff M,Dubbs HA,Izumi K,Grand K,Gray C,Mark PR,Bhoj EJ,Li D,Ortiz-Gonzalez XR,Keena B,Zackai EH,Goldberg EM,Perez de Nanclares G,Pereda A,Llano-Rivas I,Arroyo I,Fernández-Cuesta MÁ,Thauvin-Robinet C,Faivre L,Garde A,Mazel B,Bruel AL,Tress ML,Brilstra E,Fine AS,Crompton KE,Stegmann APA,Sinnema M,Stevens SCJ,Nicolai J,Lesca G,Lion-François L,Haye D,Chatron N,Piton A,Nizon M,Cogne B,Srivastava S,Bassetti J,Muss C,Gripp KW,Procopio RA,Millan F,Morrow MM,Assaf M,Moreno-De-Luca A,Joss S,Hamilton MJ,Bertoli M,Foulds N,McKee S,MacLennan AH,Gecz J,Corbett MA
R960-25 was used in Western Blotting to investigate the range of clinical phenotypes owing to disruptions of CTNNB1 to determine the association between NEDSDV and CP.
Tue Nov 01 00:00:00 UTC 2022
Subcellular proteomics of dopamine neurons in the mouse brain.
eLife
Hobson BD,Choi SJ,Mosharov EV,Soni RK,Sulzer D,Sims PA
R960-25 was used in Immunohistochemistry-immunofluorescence to find that most proteins encoded by DA neuron-enriched genes are localized within striatal dopaminergic axons, including ion channels with previously undescribed axonal localization.
Mon Jan 31 00:00:00 UTC 2022
Subcellular proteomics of dopamine neurons in the mouse brain.
eLife
Hobson BD,Choi SJ,Mosharov EV,Soni RK,Sulzer D,Sims PA
R960-25 was used in Immunohistochemistry-immunofluorescence to find that most proteins encoded by DA neuron-enriched genes are localized within striatal dopaminergic axons, including ion channels with previously undescribed axonal localization.
Mon Jan 31 00:00:00 UTC 2022
Anti-tumour immunity induces aberrant peptide presentation in melanoma.
Nature
Bartok O,Pataskar A,Nagel R,Laos M,Goldfarb E,Hayoun D,Levy R,Körner PR,Kreuger IZM,Champagne J,Zaal EA,Bleijerveld OB,Huang X,Kenski J,Wargo J,Brandis A,Levin Y,Mizrahi O,Alon M,Lebon S,Yang W,Nielsen MM,Stern-Ginossar N,Altelaar M,Berkers CR,Geiger T,Peeper DS,Olweus J,Samuels Y,Agami R
R960-25 was used in Western Blot to investigate the effects of prolonged IFNγ treatment on mRNA translation.
Mon Feb 01 00:00:00 UTC 2021
Drugs that inhibit TMEM16 proteins block SARS-CoV-2 spike-induced syncytia.
Nature
Braga L,Ali H,Secco I,Chiavacci E,Neves G,Goldhill D,Penn R,Jimenez-Guardeño JM,Ortega-Prieto AM,Bussani R,Cannatà A,Rizzari G,Collesi C,Schneider E,Arosio D,Shah AM,Barclay WS,Malim MH,Burrone J,Giacca M
R960-25 was used in Immunocytochemistry to suggest a potential mechanism for COVID-19 disease pathogenesis and support the repurposing of niclosamide for therapy.
Tue Jun 01 00:00:00 UTC 2021
Functional diversity for body actions in the mesencephalic locomotor region.
Cell
Ferreira-Pinto MJ,Kanodia H,Falasconi A,Sigrist M,Esposito MS,Arber S
R960CUS was used in Immunohistochemistry to identify and functionally dissect glutamatergic mesencephalic locomotor region subpopulations based on the premise that target connectivity might be linked to function.
Thu Aug 19 00:00:00 UTC 2021
Increased ACTL6A occupancy within mSWI/SNF chromatin remodelers drives human squamous cell carcinoma.
Molecular cell
Chang CY,Shipony Z,Lin SG,Kuo A,Xiong X,Loh KM,Greenleaf WJ,Crabtree GR
R960-25 was used in Western Blotting to conclude that ACTL6A plays a dosage-sensitive role underlying squamous cell carcinoma (SCC).
Thu Dec 16 00:00:00 UTC 2021
Integrated genomic analysis reveals key features of long undecoded transcript isoform-based gene repression.
Molecular cell
Tresenrider A,Morse K,Jorgensen V,Chia M,Liao H,van Werven FJ,Ünal E
R960-25 was used in Western Blot to conclude that LUTIs downregulate gene expression in a manner that integrates translational repression, chromatin state changes, and the magnitude of LUTI expression.
Thu May 20 00:00:00 UTC 2021
Integrated genomic analysis reveals key features of long undecoded transcript isoform-based gene repression.
Molecular cell
Tresenrider A,Morse K,Jorgensen V,Chia M,Liao H,van Werven FJ,Ünal E
R960-25 was used in Western Blot to conclude that LUTIs downregulate gene expression in a manner that integrates translational repression, chromatin state changes, and the magnitude of LUTI expression.
Thu May 20 00:00:00 UTC 2021
Functional landscape of SARS-CoV-2 cellular restriction.
Molecular cell
Martin-Sancho L,Lewinski MK,Pache L,Stoneham CA,Yin X,Becker ME,Pratt D,Churas C,Rosenthal SB,Liu S,Weston S,De Jesus PD,O'Neill AM,Gounder AP,Nguyen C,Pu Y,Curry HM,Oom AL,Miorin L,Rodriguez-Frandsen A,Zheng F,Wu C,Xiong Y,Urbanowski M,Shaw ML,Chang MW,Benner C,Hope TJ,Frieman MB,García-Sastre A,Ideker T,Hultquist JF,Guatelli J,Chanda SK
R960-25 was used in Western Blot, Immunocytochemistry to illuminate a set of IFN-stimulated genes that underlie innate immune control of SARS-CoV-2/SARS-CoV-1 infection, which will facilitate the understanding of host determinants that impact disease severity and offer therapeutic strategies for COVID-19.
Thu Jun 17 00:00:00 UTC 2021
Functional landscape of SARS-CoV-2 cellular restriction.
Molecular cell
Martin-Sancho L,Lewinski MK,Pache L,Stoneham CA,Yin X,Becker ME,Pratt D,Churas C,Rosenthal SB,Liu S,Weston S,De Jesus PD,O'Neill AM,Gounder AP,Nguyen C,Pu Y,Curry HM,Oom AL,Miorin L,Rodriguez-Frandsen A,Zheng F,Wu C,Xiong Y,Urbanowski M,Shaw ML,Chang MW,Benner C,Hope TJ,Frieman MB,García-Sastre A,Ideker T,Hultquist JF,Guatelli J,Chanda SK
R960-25 was used in Western Blot, Immunocytochemistry to illuminate a set of IFN-stimulated genes that underlie innate immune control of SARS-CoV-2/SARS-CoV-1 infection, which will facilitate the understanding of host determinants that impact disease severity and offer therapeutic strategies for COVID-19.
Thu Jun 17 00:00:00 UTC 2021
Combinatorial analysis of translation dynamics reveals eIF2 dependence of translation initiation at near-cognate codons.
Nucleic acids research
Ichihara K,Matsumoto A,Nishida H,Kito Y,Shimizu H,Shichino Y,Iwasaki S,Imami K,Ishihama Y,Nakayama KI
R960CUS was used in Immunoprecipitation to identify translation initiation sites through a new method, TISCA (TIS detection by translation Complex Analysis).
Wed Jul 21 00:00:00 UTC 2021
Chemical Inhibition of ENL/AF9 YEATS Domains in Acute Leukemia.
ACS central science
Garnar-Wortzel L,Bishop TR,Kitamura S,Milosevich N,Asiaban JN,Zhang X,Zheng Q,Chen E,Ramos AR,Ackerman CJ,Hampton EN,Chatterjee AK,Young TS,Hull MV,Sharpless KB,Cravatt BF,Wolan DW,Erb MA
R960-25 was used in Western Blot to establish YEATS domain inhibition as a viable approach to disrupt the pathogenic function of ENL in acute leukemia and provides the first thoroughly characterized chemical probe for the ENL YEATS domain.
Wed May 26 00:00:00 UTC 2021
Comparative Connectomics Reveals How Partner Identity, Location, and Activity Specify Synaptic Connectivity in Drosophila.
Neuron
Valdes-Aleman J,Fetter RD,Sales EC,Heckman EL,Venkatasubramanian L,Doe CQ,Landgraf M,Cardona A,Zlatic M
R960-25 was used in Immunohistochemistry to elucidate the roles of (1) partner identity, (2) location, and (3) activity in circuit assembly in the embryonic nerve cord of Drosophila.
Wed Jan 06 00:00:00 UTC 2021
Cell type-selective secretome profiling in vivo.
Nature chemical biology
Wei W,Riley NM,Yang AC,Kim JT,Terrell SM,Li VL,Garcia-Contreras M,Bertozzi CR,Long JZ
R960-25 was used in Western Blot to develop a method for the detection, enrichment, and profiling of cell type-selective secretomes in mice.
Mon Mar 01 00:00:00 UTC 2021
Differential compartmentalization of BMP4/NOGGIN requires NOGGIN trans-epithelial transport.
Developmental cell
Phan-Everson T,Etoc F,Li S,Khodursky S,Yoney A,Brivanlou AH,Siggia ED
R960-25 was used in Western Blot to propose that the transport of morphogen inhibitors regulates the spatial availability of morphogens during embryogenesis.
Mon Jul 12 00:00:00 UTC 2021
Toll receptors remodel epithelia by directing planar-polarized Src and PI3K activity.
Developmental cell
Tamada M,Shi J,Bourdot KS,Supriyatno S,Palmquist KH,Gutierrez-Ruiz OL,Zallen JA
R960-25 was used in Western Blot, Immunoprecipitation, Immunohistochemistry (PFA fixed) to demonstrate that Toll receptors direct cell polarity and behavior by locally mobilizing Src and PI3K activity.
Mon Jun 07 00:00:00 UTC 2021
Toll receptors remodel epithelia by directing planar-polarized Src and PI3K activity.
Developmental cell
Tamada M,Shi J,Bourdot KS,Supriyatno S,Palmquist KH,Gutierrez-Ruiz OL,Zallen JA
R960-25 was used in Western Blot, Immunoprecipitation, Immunohistochemistry (PFA fixed) to demonstrate that Toll receptors direct cell polarity and behavior by locally mobilizing Src and PI3K activity.
Mon Jun 07 00:00:00 UTC 2021
Toll receptors remodel epithelia by directing planar-polarized Src and PI3K activity.
Developmental cell
Tamada M,Shi J,Bourdot KS,Supriyatno S,Palmquist KH,Gutierrez-Ruiz OL,Zallen JA
R960-25 was used in Western Blot, Immunoprecipitation, Immunohistochemistry (PFA fixed) to demonstrate that Toll receptors direct cell polarity and behavior by locally mobilizing Src and PI3K activity.
Mon Jun 07 00:00:00 UTC 2021
USP42 drives nuclear speckle mRNA splicing via directing dynamic phase separation to promote tumorigenesis.
Cell death and differentiation
Liu S,Wang T,Shi Y,Bai L,Wang S,Guo D,Zhang Y,Qi Y,Chen C,Zhang J,Zhang Y,Liu Q,Yang Q,Wang Y,Liu H
R960-25 was used in Immunohistochemistry to show that USP42 expression is strongly correlated with that of PLRG1 in non-small-cell lung cancer samples and predicts adverse prognosis in overall survival.
Sun Aug 01 00:00:00 UTC 2021
CK2 Inhibits TIMELESS Nuclear Export and Modulates CLOCK Transcriptional Activity to Regulate Circadian Rhythms.
Current biology : CB
Cai YD,Xue Y,Truong CC,Del Carmen-Li J,Ochoa C,Vanselow JT,Murphy KA,Li YH,Liu X,Kunimoto BL,Zheng H,Zhao C,Zhang Y,Schlosser A,Chiu JC
R960-25 was used in Western Blotting to investigate the role of phosphorylation of the protein TIMELESS in modulating kinase accessibility in the CLOCK nucleus using drosophila.
Mon Feb 08 00:00:00 UTC 2021
USP42 protects ZNRF3/RNF43 from R-spondin-dependent clearance and inhibits Wnt signalling.
EMBO reports
Giebel N,de Jaime-Soguero A,García Del Arco A,Landry JJM,Tietje M,Villacorta L,Benes V,Fernández-Sáiz V,Acebrón SP
R960-25 was used in Western Blot to show that the USP42 antagonises R-spondin by protecting ZNRF3/RNF43 from ubiquitin-dependent clearance.
Wed May 05 00:00:00 UTC 2021
Disruption of RFX family transcription factors causes autism, attention-deficit/hyperactivity disorder, intellectual disability, and dysregulated behavior.
Genetics in medicine : official journal of the American College of Medical Genetics
Harris HK,Nakayama T,Lai J,Zhao B,Argyrou N,Gubbels CS,Soucy A,Genetti CA,Suslovitch V,Rodan LH,Tiller GE,Lesca G,Gripp KW,Asadollahi R,Hamosh A,Applegate CD,Turnpenny PD,Simon MEH,Volker-Touw CML,Gassen KLIV,Binsbergen EV,Pfundt R,Gardeitchik T,Vries BBA,Immken LL,Buchanan C,Willing M,Toler TL,Fassi E,Baker L,Vansenne F,Wang X,Ambrus JL,Fannemel M,Posey JE,Agolini E,Novelli A,Rauch A,Boonsawat P,Fagerberg CR,Larsen MJ,Kibaek M,Labalme A,Poisson A,Payne KK,Walsh LE,Aldinger KA,Balciuniene J,Skraban C,Gray C,Murrell J,Bupp CP,Pascolini G,Grammatico P,Broly M,Küry S,Nizon M,Rasool IG,Zahoor MY,Kraus C,Reis A,Iqbal M,Uguen K,Audebert-Bellanger S,Ferec C,Redon S,Baker J,Wu Y,Zampino G,Syrbe S,Brosse I,Jamra RA,Dobyns WB,Cohen LL,Blomhoff A,Mignot C,Keren B,Courtin T,Agrawal PB,Beggs AH,Yu TW
R960-25 was used in Western Blot to describe a novel neurobehavioral phenotype of autism spectrum disorder (ASD), intellectual disability, and/or attention-deficit/hyperactivity disorder (ADHD) associated with de novo or inherited deleterious variants in members of the RFX family of genes.
Tue Jun 01 00:00:00 UTC 2021
DOT1L modulates the senescence-associated secretory phenotype through epigenetic regulation of IL1A.
The Journal of cell biology
Leon KE,Buj R,Lesko E,Dahl ES,Chen CW,Tangudu NK,Imamura-Kawasawa Y,Kossenkov AV,Hobbs RP,Aird KM
R960-25 was used in Western Blot to establish DOT1L as an epigenetic regulator of the SASP, whose expression is uncoupled from the senescence-associated cell cycle arrest.
Mon Aug 02 00:00:00 UTC 2021
Neuronal activity regulates the nuclear proteome to promote activity-dependent transcription.
The Journal of cell biology
Herbst WA,Deng W,Wohlschlegel JA,Achiro JM,Martin KC
R960-25 was used in Immunocytochemistry to highlight the importance of the nuclear proteasome in regulating the activity-dependent nuclear proteome and point to a specific role for PDCD4 as a regulator of activity-dependent transcription in neurons.
Mon Dec 06 00:00:00 UTC 2021
Interactions of SARS-CoV-2 envelope protein with amilorides correlate with antiviral activity.
PLoS pathogens
Park SH,Siddiqi H,Castro DV,De Angelis AA,Oom AL,Stoneham CA,Lewinski MK,Clark AE,Croker BA,Carlin AF,Guatelli J,Opella SJ
R960-25 was used in Western Blot to describe the structure and dynamics of the SARS-CoV-2 envelope protein using NMR spectroscopy.
Sat May 01 00:00:00 UTC 2021
Activation of EphA2-EGFR signaling in oral epithelial cells by Candida albicans virulence factors.
PLoS pathogens
Swidergall M,Solis NV,Millet N,Huang MY,Lin J,Phan QT,Lazarus MD,Wang Z,Yeaman MR,Mitchell AP,Filler SG
R960-25 was used in Dot blot to study the interactions among Als3, candidalysin, ephrin type-A receptor 2, and epidermal growth factor receptor during C. albicans infection of oral epithelial cells.
Fri Jan 01 00:00:00 UTC 2021
TGA class II transcription factors are essential to restrict oxidative stress in response to UV-B stress in Arabidopsis.
Journal of experimental botany
Herrera-Vásquez A,Fonseca A,Ugalde JM,Lamig L,Seguel A,Moyano TC,Gutiérrez RA,Salinas P,Vidal EA,Holuigue L
R960-25 was used in Western Blotting to present evident to support a role for TGA class II transcription factors as critical regulators of genes controlling Reactive Oxygen Species (ROS) levels in the tolerance response to UV-B stress in Arabidopsis.
Sat Feb 27 00:00:00 UTC 2021
Endocytosis mediated by an atypical CUBAM complex modulates slit diaphragm dynamics in nephrocytes.
Development (Cambridge, England)
Atienza-Manuel A,Castillo-Mancho V,De Renzis S,Culi J,Ruiz-Gómez M
R960-25 was used in Immunohistochemistry to argue for a possible unexpected conserved role of this endocytic receptor in vertebrate SD remodelling.
Mon Nov 15 00:00:00 UTC 2021
SARS-CoV-2 Nonstructural Protein 1 Inhibits the Interferon Response by Causing Depletion of Key Host Signaling Factors.
Journal of virology
Kumar A,Ishida R,Strilets T,Cole J,Lopez-Orozco J,Fayad N,Felix-Lopez A,Elaish M,Evseev D,Magor KE,Mahal LK,Nagata LP,Evans DH,Hobman TC
R960-25 was used in Western Blot to provide new insights on how SARS-CoV-2 evades the IFN response to successfully establish infection.
Thu Jun 10 00:00:00 UTC 2021
An Integrated Systems Biology Approach Identifies the Proteasome as A Critical Host Machinery for ZIKV and DENV Replication.
Genomics, proteomics & bioinformatics
Song G,Lee EM,Pan J,Xu M,Rho HS,Cheng Y,Whitt N,Yang S,Kouznetsova J,Klumpp-Thomas C,Michael SG,Moore C,Yoon KJ,Christian KM,Simeonov A,Huang W,Xia M,Huang R,Lal-Nag M,Tang H,Zheng W,Qian J,Song H,Ming GL,Zhu H
R960-25 was used in Western Blot to develop a systematic understanding of virus-host protein-protein interaction networks can reveal cellular pathways critical to viral replication and disease pathogenesis.
Mon Feb 01 00:00:00 UTC 2021
The Antipsychotic Drug Clozapine Suppresses the RGS4 Polyubiquitylation and Proteasomal Degradation Mediated by the Arg/N-Degron Pathway.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics
Jeon JH,Oh TR,Park S,Huh S,Kim JH,Mai BK,Lee JH,Kim SH,Lee MJ
R960-25 was used in Western Blotting to imply that modulation of protein post-translational modifications, in particular the Arg/N-degron pathway, may be a novel molecular therapeutic strategy against schizophrenia.
Thu Jul 01 00:00:00 UTC 2021
TBL1XR1 Ensures Balanced Neural Development Through NCOR Complex-Mediated Regulation of the MAPK Pathway.
Frontiers in cell and developmental biology
Mastrototaro G,Zaghi M,Massimino L,Moneta M,Mohammadi N,Banfi F,Bellini E,Indrigo M,Fagnocchi G,Bagliani A,Taverna S,Rohm M,Herzig S,Sessa A
R960-25 was used in Immunocytochemistry to show that Tbl1xr1 knock-out mice exhibit behavioral and neuronal abnormalities.
Sat Mar 13 00:00:00 UTC 2021
Large Multicohort Study Reveals a Prostate Cancer Susceptibility Allele at 5p15 Regulating TERT via Androgen Signaling-Orchestrated Chromatin Binding of E2F1 and MYC.
Frontiers in oncology
Dong X,Zhang Q,Hao J,Xie Q,Xu B,Zhang P,Lu H,Huang Q,Yang T,Wei GH,Na R,Gao P
R960-25 was used in Western Blotting to investigate the roles of noncoding variations coordinated with androgen signaling and oncogenic transcription factors in mis-regulating TERT expression and driving PCa.
Mon Aug 19 00:00:00 UTC 2024
The nuclear activity of the actin-binding Moesin protein is necessary for gene expression in Drosophila.
The FEBS journal
Bajusz C,Kristó I,Abonyi C,Venit T,Vedelek V,Lukácsovich T,Farkas A,Borkúti P,Kovács Z,Bajusz I,Marton A,Vizler C,Lipinszki Z,Sinka R,Percipalle P,Vilmos P
R960-25 was used in Immunohistochemistry to reveal that in Drosophila the expression of protein chaperones requires the nuclear functions of Moesin.
Sun Aug 01 00:00:00 UTC 2021
NAA10 p.(N101K) disrupts N-terminal acetyltransferase complex NatA and is associated with developmental delay and hemihypertrophy.
European journal of human genetics : EJHG
McTiernan N,Gill H,Prada CE,Pachajoa H,Lores J,Arnesen T
R960-25 was used in Western Blotting to identify the previously undescribed NAA10 c.303C>A and c.303C>G p.(N101K) variants in two unrelated girls.
Mon Feb 01 00:00:00 UTC 2021
Turgor-dependent and coronin-mediated F-actin dynamics drive septin disc-to-ring remodeling in the blast fungus Magnaporthe oryzae.
Journal of cell science
Dulal N,Rogers AM,Proko R,Bieger BD,Liyanage R,Krishnamurthi VR,Wang Y,Egan MJ
R960-25 was used in Western Blot to show F-actin rings are recruited to the circumference of incipient septin disc-like structures, necessary for their contraction and remodelling into rings.
Thu Feb 25 00:00:00 UTC 2021
WNT-responsive SUMOylation of ZIC5 promotes murine neural crest cell development, having multiple effects on transcription.
Journal of cell science
Ali RG,Bellchambers HM,Warr N,Ahmed JN,Barratt KS,Neill K,Diamand KEM,Arkell RM
R960-25 was used in Western Blot to demonstrate that WNT-dependent SUMOylation influences Zic protein activity during neural crest cell specification.
Sat May 01 00:00:00 UTC 2021
C9orf72-associated arginine-rich dipeptide repeats induce RNA-dependent nuclear accumulation of Staufen in neurons.
Human molecular genetics
Kim ES,Chung CG,Park JH,Ko BS,Park SS,Kim YH,Cha IJ,Kim J,Ha CM,Kim HJ,Lee SB
R960-25 was used in Immunohistochemistry to show that nuclear accumulation of Staufen in neurons may be an important pathological feature contributing to the pathogenesis of ALS/FTD.
Wed Jun 09 00:00:00 UTC 2021
C9orf72-associated arginine-rich dipeptide repeats induce RNA-dependent nuclear accumulation of Staufen in neurons.
Human molecular genetics
Kim ES,Chung CG,Park JH,Ko BS,Park SS,Kim YH,Cha IJ,Kim J,Ha CM,Kim HJ,Lee SB
R960-25 was used in Immunohistochemistry to show that nuclear accumulation of Staufen in neurons may be an important pathological feature contributing to the pathogenesis of ALS/FTD.
Wed Jun 09 00:00:00 UTC 2021
eVIP2: Expression-based variant impact phenotyping to predict the function of gene variants.
PLoS computational biology
Thornton AM,Fang L,Lo A,McSharry M,Haan D,O'Brien C,Berger AH,Giannakis M,Brooks AN
R960-25 was used in Western Blot to suggest that the eVIP2 method is an important step towards overcoming the current challenge of variant interpretation in the implementation of precision medicine.
Thu Jul 01 00:00:00 UTC 2021
Functional evaluation of 16 SCHAD missense variants: Only amino acid substitutions causing congenital hyperinsulinism of infancy lead to loss-of-function phenotypes in vitro.
Journal of inherited metabolic disease
Velasco K,St-Louis JL,Hovland HN,Thompson N,Ottesen Å,Choi MH,Pedersen L,Njølstad PR,Arnesen T,Fjeld K,Aukrust I,Myklebust LM,Molven A
R960-25 was used in Western Blot to functionally assess naturally occurring short-chain 3-hydroxyacyl-CoA dehydrogenase (SCHAD) missense variants.
Fri Jan 01 00:00:00 UTC 2021
A novel allele of SIR2 reveals a heritable intermediate state of gene silencing.
Genetics
Farris D,Saxton DS,Rine J
R960-25 was used in Western Blot to reveal a heritable state of intermediate silencing and linked this state to a central silencing factor, Sir2.
Mon May 17 00:00:00 UTC 2021
Syntaxins 6 and 8 facilitate tau into secretory pathways.
The Biochemical journal
Lee WS,Tan DC,Deng Y,van Hummel A,Ippati S,Stevens C,Carmona-Mora P,Ariawan D,Hou L,Stefen H,Tomanic T,Bi M,Tomasetig F,Martin A,Fath T,Palmer S,Ke YD,Ittner LM
R960CUS was used in Immunoprecipitation to study the interactome of the C-terminal tail region of tau and show syntaxin 8 (STX8) as a mediator of tau release from cells.
Fri Apr 16 00:00:00 UTC 2021
Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002.
Photosynthesis research
Dahlgren KK,Gates C,Lee T,Cameron JC
R960-25 was used in Western Blotting to demonstrate the power of APEX2 as a tool to study the cell biology of intracellular features and processes, including photosystem II assembly in cyanobacteria, with enhanced spatiotemporal resolution.
Mon Feb 01 00:00:00 UTC 2021
MCM complex members MCM3 and MCM7 are associated with a phenotypic spectrum from Meier-Gorlin syndrome to lipodystrophy and adrenal insufficiency.
European journal of human genetics : EJHG
Knapp KM,Jenkins DE,Sullivan R,Harms FL,von Elsner L,Ockeloen CW,de Munnik S,Bongers EMHF,Murray J,Pachter N,Denecke J,Kutsche K,Bicknell LS
R960-25 was used in Western Blotting to report the identification of variants in both MCM3 and MCM7 in individuals with either MGORS or an overlapping congenital disorder.
Thu Jul 01 00:00:00 UTC 2021
Cytoplasmic dynein-1 cargo diversity is mediated by the combinatorial assembly of FTS-Hook-FHIP complexes.
eLife
Christensen JR,Kendrick AA,Truong JB,Aguilar-Maldonado A,Adani V,Dzieciatkowska M,Reck-Peterson SL
R960-25 was used in Western Blotting to suggest that combinatorial assembly of different FTS-Hook-FHIP complexes is one mechanism dynein uses to achieve cargo specificity.
Thu Dec 09 00:00:00 UTC 2021
FBXO7 triggers caspase 8-mediated proteolysis of the transcription factor FOXO4 and exacerbates neuronal cytotoxicity.
The Journal of biological chemistry
Lee SH,Jung S,Lee YJ,Hyun M,Chung KC
R960-25 was used in Western Blot to suggest that FOXO4 is negatively regulated in FBXO7-linked PD through caspase 8 activation, suppressing the cytoprotective effect of FOXO4 during 6-OHDA-induced neuronal cell death.
Wed Dec 01 00:00:00 UTC 2021
The middle lipin domain adopts a membrane-binding dimeric protein fold.
Nature communications
Gu W,Gao S,Wang H,Fleming KD,Hoffmann RM,Yang JW,Patel NM,Choi YM,Burke JE,Reue K,Airola MV
R960-25 was used in Immunoprecipitation and Western Blotting to demonstrate that mechanical forces shift fibroblasts toward pro-fibrotic phenotypes driven by ERK-YAP activation, leading to myofibroblast differentiation and excessive collagen production.
Thu Aug 05 00:00:00 UTC 2021
The middle lipin domain adopts a membrane-binding dimeric protein fold.
Nature communications
Gu W,Gao S,Wang H,Fleming KD,Hoffmann RM,Yang JW,Patel NM,Choi YM,Burke JE,Reue K,Airola MV
R960-25 was used in Immunoprecipitation and Western Blotting to demonstrate that mechanical forces shift fibroblasts toward pro-fibrotic phenotypes driven by ERK-YAP activation, leading to myofibroblast differentiation and excessive collagen production.
Thu Aug 05 00:00:00 UTC 2021
Target identification for small-molecule discovery in the FOXO3a tumor-suppressor pathway using a biodiverse peptide library.
Cell chemical biology
Emery A,Hardwick BS,Crooks AT,Milech N,Watt PM,Mithra C,Kumar V,Giridharan S,Sadasivam G,Mathivanan S,Sudhakar S,Bairy S,Bharatham K,Hurakadli MA,Prasad TK,Kamariah N,Muellner M,Coelho M,Torrance CJ,McKenzie GJ,Venkitaraman AR
R960-25 was used in Immunoprecipitation to exemplify "protein interference," an approach using evolutionarily diverse, natural peptides to rapidly identify, validate, and develop chemical probes against MMIs essential for complex cellular phenotypes.
Thu Nov 18 00:00:00 UTC 2021
ER - lysosome contacts at a pre-axonal region regulate axonal lysosome availability.
Nature communications
Özkan N,Koppers M,van Soest I,van Harten A,Jurriens D,Liv N,Klumperman J,Kapitein LC,Hoogenraad CC,Farías GG
R960-25 was used in Immunocytochemistry to investigate if endoplasmic reticulum tubule - lysosome contacts at a pre-axonal region finely orchestrate axonal lysosome availability for proper neuronal function.
Fri Jul 23 00:00:00 UTC 2021
K63-linked ubiquitination of DYRK1A by TRAF2 alleviates Sprouty 2-mediated degradation of EGFR.
Cell death & disease
Zhang P,Zhang Z,Fu Y,Zhang Y,Washburn MP,Florens L,Wu M,Huang C,Hou Z,Mohan M
R960-25 was used in Immunoprecipitation to suggest that the axis of TRAF2-DYRK1A-Sprouty 2 can be a target for new therapeutic development for EGFR-mediated human pathologies.
Fri Jun 11 00:00:00 UTC 2021
Modulating HSF1 levels impacts expression of the estrogen receptor α and antiestrogen response.
Life science alliance
Silveira MA,Tav C,Bérube-Simard FA,Cuppens T,Leclercq M,Fournier É,Côté MC,Droit A,Bilodeau S
R960-25 was used in Western Blot to show that high levels of the stress response master regulator, the heat shock factor 1 (HSF1), are associated with antiestrogen resistance in breast cancer cells.
Sat May 01 00:00:00 UTC 2021
TM9SF4 is a novel regulator in lineage commitment of bone marrow mesenchymal stem cells to either osteoblasts or adipocytes.
Stem cell research & therapy
Yu L,Xie M,Zhang F,Wan C,Yao X
R960-25 was used in Immunocytochemistry-immunofluorescence to investigate the function of TM9SF4 in MSC differentiation commitment, as well as its role in osteoporosis.
Sat Nov 13 00:00:00 UTC 2021
A high-throughput screen for TMPRSS2 expression identifies FDA-approved compounds that can limit SARS-CoV-2 entry.
Nature communications
Chen Y,Lear TB,Evankovich JW,Larsen MB,Lin B,Alfaras I,Kennerdell JR,Salminen L,Camarco DP,Lockwood KC,Tuncer F,Liu J,Myerburg MM,McDyer JF,Liu Y,Finkel T,Chen BB
R960-25 was used in Western Blot to identify small molecules that reduce surface expression of TMPRSS2.
Wed Jun 23 00:00:00 UTC 2021
Affinity maturation of the RLIP76 Ral binding domain to inform the design of stapled peptides targeting the Ral GTPases.
The Journal of biological chemistry
Hurd CA,Brear P,Revell J,Ross S,Mott HR,Owen D
R960-25 was used in ChIP assay to demonstrate if manipulation of a native binding partner can assist in the rational design of stapled peptide inhibitors targeting a protein-protein interaction.
Tue Aug 24 00:00:00 UTC 2021
A Fbxo48 inhibitor prevents pAMPKα degradation and ameliorates insulin resistance.
Nature chemical biology
Liu Y,Jurczak MJ,Lear TB,Lin B,Larsen MB,Kennerdell JR,Chen Y,Huckestein BR,Nguyen MK,Tuncer F,Jiang Y,Monga SP,O'Donnell CP,Finkel T,Chen BB,Mallampalli RK
R960-25 was used in Western Blotting to provide a unique bioactive compound that inhibits pAmpkα disposal, defining a new pathway regulating Ampk biological activity and demonstrate the potential utility of modulating this pathway for therapeutic benefit.
Mon Mar 01 00:00:00 UTC 2021
FKBP39 controls nutrient dependent Nprl3 expression and TORC1 activity in Drosophila.
Cell death & disease
Zhou Y,Guo J,Wang X,Cheng Y,Guan J,Barman P,Sun MA,Fu Y,Wei W,Feng C,Lilly MA,Wei Y
R960-25 was used in Western Blot to show that the 5'UTR of nprl3 transcripts contain a functional upstream open reading frame (uORF) that inhibits main ORF translation.
Wed Jun 02 00:00:00 UTC 2021
Characterization of HNRNPA1 mutations defines diversity in pathogenic mechanisms and clinical presentation.
JCI insight
Beijer D,Kim HJ,Guo L,O'Donovan K,Mademan I,Deconinck T,Van Schil K,Fare CM,Drake LE,Ford AF,Kochański A,Kabzińska D,Dubuisson N,Van den Bergh P,Voermans NC,Lemmers RJ,van der Maarel SM,Bonner D,Sampson JB,Wheeler MT,Mehrabyan A,Palmer S,De Jonghe P,Shorter J,Taylor JP,Baets J
R960-25 was used in Western Blot to characterize 4 potentially novel HNRNPA1 mutations and 2 known HNRNPA1 mutations previously connected to ALS and MSP, in a broad spectrum of patients with hereditary motor neuropathy, ALS, and myopathy.
Thu Jul 22 00:00:00 UTC 2021
Frame-shifted APOBEC3A encodes two alternative proapoptotic proteins that target the mitochondrial network.
The Journal of biological chemistry
Caval V,Suspène R,Khalfi P,Gaillard J,Caignard G,Vitour D,Roingeard P,Vartanian JP,Wain-Hobson S
R960-25 was used in Immunocytochemistry to investigate the expression of two novel alternative "A3Alt" proteins encoded in the +1-shifted reading frame of the APOBEC3A gene.
Wed Sep 01 00:00:00 UTC 2021
The histone chaperone Spt6 is required for normal recruitment of the capping enzyme Abd1 to transcribed regions.
The Journal of biological chemistry
Gopalakrishnan R,Winston F
R960-25 was used in ChIP assay, Western Blot to characterize the differences in polymerase II (RNAPII) interacting factors between wildtype cells and those depleted of the histone chaperone Spt6, leading to the identification of proteins that depend on Spt6 for their interaction with RNAPII.
Fri Oct 01 00:00:00 UTC 2021
The histone chaperone Spt6 is required for normal recruitment of the capping enzyme Abd1 to transcribed regions.
The Journal of biological chemistry
Gopalakrishnan R,Winston F
R960-25 was used in ChIP assay, Western Blot to characterize the differences in polymerase II (RNAPII) interacting factors between wildtype cells and those depleted of the histone chaperone Spt6, leading to the identification of proteins that depend on Spt6 for their interaction with RNAPII.
Fri Oct 01 00:00:00 UTC 2021
Structure of PDE3A-SLFN12 complex reveals requirements for activation of SLFN12 RNase.
Nature communications
Garvie CW,Wu X,Papanastasiou M,Lee S,Fuller J,Schnitzler GR,Horner SW,Baker A,Zhang T,Mullahoo JP,Westlake L,Hoyt SH,Toetzl M,Ranaghan MJ,de Waal L,McGaunn J,Kaplan B,Piccioni F,Yang X,Lange M,Tersteegen A,Raymond D,Lewis TA,Carr SA,Cherniack AD,Lemke CT,Meyerson M,Greulich H
R960-25 was used in Western Blot to facilitate development of velcrin compounds into new cancer therapies.
Fri Jul 16 00:00:00 UTC 2021
ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity.
Nature microbiology
Liu G,Lee JH,Parker ZM,Acharya D,Chiang JJ,van Gent M,Riedl W,Davis-Gardner ME,Wies E,Chiang C,Gack MU
R960-25 was used in Immunoprecipitation, Western Blot to investigate the role of interferon (IFN)-stimulated gene 15 in innate immunity.
Thu Apr 01 00:00:00 UTC 2021
ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity.
Nature microbiology
Liu G,Lee JH,Parker ZM,Acharya D,Chiang JJ,van Gent M,Riedl W,Davis-Gardner ME,Wies E,Chiang C,Gack MU
R960-25 was used in Immunoprecipitation, Western Blot to investigate the role of interferon (IFN)-stimulated gene 15 in innate immunity.
Thu Apr 01 00:00:00 UTC 2021
Cross-compartment signal propagation in the mitotic exit network.
eLife
Zhou X,Li W,Liu Y,Amon A
R960-25 was used in Western Blot to show that the MEN activating signal at SPBs is relayed to Cdc14 in the nucleolus through the dynamic localization of its terminal kinase complex Dbf2-Mob1.
Fri Jan 22 00:00:00 UTC 2021
A proximity proteomics screen in three-dimensional spheroid cultures identifies novel regulators of lumen formation.
Scientific reports
Wang LT,Proulx MÈ,Kim AD,Lelarge V,McCaffrey L
R960-25 was used in Immunocytochemistry-immunofluorescence to represent an applicable approach to explore protein-protein interactions in three-dimensional culture and to identify proteins important for lumen maintenance in normal and oncogene-expressing cells.
Tue Nov 23 00:00:00 UTC 2021
A proximity proteomics screen in three-dimensional spheroid cultures identifies novel regulators of lumen formation.
Scientific reports
Wang LT,Proulx MÈ,Kim AD,Lelarge V,McCaffrey L
R960-25 was used in Immunocytochemistry-immunofluorescence to represent an applicable approach to explore protein-protein interactions in three-dimensional culture and to identify proteins important for lumen maintenance in normal and oncogene-expressing cells.
Tue Nov 23 00:00:00 UTC 2021
SPARK: A Transcriptional Assay for Recording Protein-Protein Interactions in a Defined Time Window.
Current protocols
Geng L,Kroning KE,Wang W
R960-25 was used in Immunocytochemistry-immunoflourescence to describe the procedures for using SPARK assays to study a dynamic PPI of interest, including designing DNA constructs and optimization in HEK293T/17 cell cultures.
Thu Jul 01 00:00:00 UTC 2021
The G127V variant of the prion protein interferes with dimer formation in vitro but not in cellulo.
Scientific reports
Sangeetham SB,Engelke AD,Fodor E,Krausz SL,Tatzelt J,Welker E
R960CUS was used in Western Blot to show that while molecular dynamics may capture the features affecting dimerization in vitro, G127V inhibiting dimer formation of PrP, these are not evidenced in a more complex cellular system.
Thu Feb 04 00:00:00 UTC 2021
Two Cases of anti-PIT-1 Hypophysitis Exhibited as a Form of Paraneoplastic Syndrome not Associated With Thymoma.
Journal of the Endocrine Society
Kanie K,Iguchi G,Inuzuka M,Sakaki K,Bando H,Urai S,Shichi H,Fujita Y,Matsumoto R,Suda K,Yamamoto M,Fukuoka H,Taniguchi T,Ogawa W,Takahashi Y
R960-25 was used in Western Blotting to show that anti-PIT-1 hypophysitis is associated not only with thymoma but also with other malignancies.
Mon Mar 01 00:00:00 UTC 2021
PIWI proteomics identifies Atari and Pasilla as piRNA biogenesis factors in Aedes mosquitoes.
Cell reports
Joosten J,Taşköprü E,Jansen PWTC,Pennings B,Vermeulen M,Van Rij RP
R960-25 was used in Western Blotting to map the interactomes of the somatic PIWI proteins Ago3, Piwi4, Piwi5, and Piwi6 and identify numerous specific interactors as well as cofactors associated with multiple PIWI proteins.
Tue May 04 00:00:00 UTC 2021
The Small t Antigen of JC Virus Antagonizes RIG-I-Mediated Innate Immunity by Inhibiting TRIM25's RNA Binding Ability.
mBio
Chiang C,Dvorkin S,Chiang JJ,Potter RB,Gack MU
R960-25 was used in Western Blot to define the intracellular innate immune sensors responsible for controlling JC polyomavirus (JCV) infection and also demonstrate a novel mechanism by which a JCV-encoded protein acts as an antagonist of the type I interferon-mediated innate immune response.
Tue Apr 13 00:00:00 UTC 2021
Probing ion channel neighborhoods using proximity proteomics.
Methods in enzymology
Redel-Traub G,Liu G,Marx SO,Kushner J
R960-25 was used in Immunocytochemistry-immunofluorescence to describe the use of proximity labeling to elucidate the cardiac CaV1.2 macromolecular complex under basal conditions and after β-adrenergic stimulation.
Fri Jun 25 00:00:00 UTC 2021
BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae.
eLife
Donczew R,Hahn S
R960-25 was used in Western Blot to establish if Bdf1/2 is critical for yeast transcription and if it provides important mechanistic insights into the function of human bromodomain and extra-terminal domain proteins in all eukaryotes.
Thu Jun 17 00:00:00 UTC 2021
Protocol for cell type-specific labeling, enrichment, and proteomic profiling of plasma proteins in mice.
STAR protocols
Wei W,Riley NM,Lyu X,Bertozzi CR,Long JZ
R960-25 was used in Western Blotting to investigate adeno-associated virus for plasma protein profiling.
Fri Dec 17 00:00:00 UTC 2021
Full-length isoform transcriptome of the developing human brain provides further insights into autism.
Cell reports
Chau KK,Zhang P,Urresti J,Amar M,Pramod AB,Chen J,Thomas A,Corominas R,Lin GN,Iakoucheva LM
R960-25 was used in Western Blotting to examine the relationships between splicing isoform expression in the brain and de novo loss-of-function mutations from individuals with NDDs.
Tue Aug 31 00:00:00 UTC 2021
Transcription levels of a noncoding RNA orchestrate opposing regulatory and cell fate outcomes in yeast.
Cell reports
Moretto F,Wood NE,Chia M,Li C,Luscombe NM,van Werven FJ
R960-25 was used in Western Blotting to report that, in S. cerevisiae, the levels of transcription through a noncoding region, IRT2, located upstream in the promoter of the inducer of meiosis, IME1, regulate opposing chromatin and transcription states.
Tue Jan 19 00:00:00 UTC 2021
Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses.
Frontiers in immunology
Gao W,Rui Y,Li G,Zhai C,Su J,Liu H,Zheng W,Zheng B,Zhang W,Yang Y,Hua S,Yu X
R960-25 was used in Immunoprecipitation to demonstrate that deubiquitinating enzymes are potent inhibitors of viral evasion of host restriction.
Wed Jan 05 00:00:00 UTC 2022
Action potential-coupled Rho GTPase signaling drives presynaptic plasticity.
eLife
O'Neil SD,Rácz B,Brown WE,Gao Y,Soderblom EJ,Yasuda R,Soderling SH
R960-25 was used in Immunocytochemistry to uncover a previously unrecognized mechanism of actin-regulated short-term presynaptic plasticity that is conserved across excitatory and inhibitory terminals.
Fri Jul 16 00:00:00 UTC 2021
Noncanonical transnitrosylation network contributes to synapse loss in Alzheimer's disease.
Science (New York, N.Y.)
Nakamura T,Oh CK,Liao L,Zhang X,Lopez KM,Gibbs D,Deal AK,Scott HR,Spencer B,Masliah E,Rissman RA,Yates JR,Lipton SA
R960-25 was used in Western Blotting to conclude that enzymes with distinct primary reaction mechanisms can form a completely separate network for aberrant transnitrosylation.
Fri Jan 15 00:00:00 UTC 2021
Visualization of Host Cell Kinase Activation by Viral Proteins Using GFP Fluorescence Complementation and Immunofluorescence Microscopy.
Bio-protocol
Shu ST,Li WF,Smithgall TE
R960-25 was used in Immunocytochemistry-immunoflourescence to show that this method has the potential to reveal changes in steady-state non-receptor tyrosine kinase activity and subcellular localization in a cell population in response to other protein-kinase interactions, information that is not attainable from immunoblotting or other in vitro methods.
Mon Jul 05 00:00:00 UTC 2021
Conserved residues in the extracellular loop 2 regulate Stachel-mediated activation of ADGRG2.
Scientific reports
Gad AA,Azimzadeh P,Balenga N
R960-25 was used in ELISA to elucidate the role of extracellular loop 2 residues in ADGRG2 signaling pathways.
Wed Jul 07 00:00:00 UTC 2021
Stearic acid blunts growth-factor signaling via oleoylation of GNAI proteins.
Nature communications
Nůsková H,Serebryakova MV,Ferrer-Caelles A,Sachsenheimer T,Lüchtenborg C,Miller AK,Brügger B,Kordyukova LV,Teleman AA
R960-25 was used in Western Blot to provide a molecular mechanism for the anti-tumor effects of C18:0.
Wed Jul 28 00:00:00 UTC 2021
Electrical synaptic transmission requires a postsynaptic scaffolding protein.
eLife
Lasseigne AM,Echeverry FA,Ijaz S,Michel JC,Martin EA,Marsh AJ,Trujillo E,Marsden KC,Pereda AE,Miller AC
R960-25 was used in Immunohistochemistry to investigate the functional role of Zonula Occludens 1 in zebrafish by examining identifiable synaptic contacts of the Mauthner cell which forms stereotyped electrical synapses accessible to genetic, biochemical, cell biological, electrophysiological, and behavioral analyses.
Wed Apr 28 00:00:00 UTC 2021
Spatiotemporal dynamics of SETD5-containing NCoR-HDAC3 complex determines enhancer activation for adipogenesis.
Nature communications
Matsumura Y,Ito R,Yajima A,Yamaguchi R,Tanaka T,Kawamura T,Magoori K,Abe Y,Uchida A,Yoneshiro T,Hirakawa H,Zhang J,Arai M,Yang C,Yang G,Takahashi H,Fujihashi H,Nakaki R,Yamamoto S,Ota S,Tsutsumi S,Inoue SI,Kimura H,Wada Y,Kodama T,Inagaki T,Osborne TF,Aburatani H,Node K,Sakai J
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, ChIP Assay to investigate SET domain proteins in histone acetylation of adipogenic regulatory genes.
Thu Dec 02 00:00:00 UTC 2021
Spatiotemporal dynamics of SETD5-containing NCoR-HDAC3 complex determines enhancer activation for adipogenesis.
Nature communications
Matsumura Y,Ito R,Yajima A,Yamaguchi R,Tanaka T,Kawamura T,Magoori K,Abe Y,Uchida A,Yoneshiro T,Hirakawa H,Zhang J,Arai M,Yang C,Yang G,Takahashi H,Fujihashi H,Nakaki R,Yamamoto S,Ota S,Tsutsumi S,Inoue SI,Kimura H,Wada Y,Kodama T,Inagaki T,Osborne TF,Aburatani H,Node K,Sakai J
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, ChIP Assay to investigate SET domain proteins in histone acetylation of adipogenic regulatory genes.
Thu Dec 02 00:00:00 UTC 2021
Spatiotemporal dynamics of SETD5-containing NCoR-HDAC3 complex determines enhancer activation for adipogenesis.
Nature communications
Matsumura Y,Ito R,Yajima A,Yamaguchi R,Tanaka T,Kawamura T,Magoori K,Abe Y,Uchida A,Yoneshiro T,Hirakawa H,Zhang J,Arai M,Yang C,Yang G,Takahashi H,Fujihashi H,Nakaki R,Yamamoto S,Ota S,Tsutsumi S,Inoue SI,Kimura H,Wada Y,Kodama T,Inagaki T,Osborne TF,Aburatani H,Node K,Sakai J
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, ChIP Assay to investigate SET domain proteins in histone acetylation of adipogenic regulatory genes.
Thu Dec 02 00:00:00 UTC 2021
Functional interpretation of ATAD3A variants in neuro-mitochondrial phenotypes.
Genome medicine
Yap ZY,Park YH,Wortmann SB,Gunning AC,Ezer S,Lee S,Duraine L,Wilichowski E,Wilson K,Mayr JA,Wagner M,Li H,Kini U,Black ED,Monaghan KG,Lupski JR,Ellard S,Westphal DS,Harel T,Yoon WH
R960-25 was used in Western Blot to expand the allelic spectrum of ATAD3A variants and exemplify the use of a functional assay in Drosophila to aid variant interpretation.
Mon Apr 12 00:00:00 UTC 2021
Protein disulfide isomerase ERp57 protects early muscle denervation in experimental ALS.
Acta neuropathologica communications
Rozas P,Pinto C,Martínez Traub F,Díaz R,Pérez V,Becerra D,Ojeda P,Ojeda J,Wright MT,Mella J,Plate L,Henríquez JP,Hetz C,Medinas DB
R960-25 was used in Western Blot to study the consequences of overexpressing ERp57 in experimental amyotrophic lateral sclerosis (ALS) using mutant superoxide dismutase 1 (SOD1) mice.
Thu Feb 04 00:00:00 UTC 2021
Drosophila ßHeavy-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil.
Nature communications
Pogodalla N,Kranenburg H,Rey S,Rodrigues S,Cardona A,Klämbt C
R960-25 was used in Immunohistochemistry to conclude that polarized glia compartmentalizes the brain and is essential for proper nervous system function.
Thu Nov 04 00:00:00 UTC 2021
Structural and thermodynamical insights into the binding and inhibition of FIH-1 by the N-terminal disordered region of Mint3.
The Journal of biological chemistry
Ten T,Nagatoishi S,Maeda R,Hoshino M,Nakayama Y,Seiki M,Sakamoto T,Tsumoto K
R960-25 was used in Western Blotting to clarify the structural and thermodynamical changes of Mint3 binding to FIH-1. First, using a combination of circular dichroism, nuclear magnetic resonance, and hydrogen/deuterium exchange-mass spectrometry (HDX-MS).
Mon Nov 01 00:00:00 UTC 2021
Fatal perinatal mitochondrial cardiac failure caused by recurrent de novo duplications in the ATAD3 locus.
Med (New York, N.Y.)
Frazier AE,Compton AG,Kishita Y,Hock DH,Welch AE,Amarasekera SSC,Rius R,Formosa LE,Imai-Okazaki A,Francis D,Wang M,Lake NJ,Tregoning S,Jabbari JS,Lucattini A,Nitta KR,Ohtake A,Murayama K,Amor DJ,McGillivray G,Wong FY,van der Knaap MS,Jeroen Vermeulen R,Wiltshire EJ,Fletcher JM,Lewis B,Baynam G,Ellaway C,Balasubramaniam S,Bhattacharya K,Freckmann ML,Arbuckle S,Rodriguez M,Taft RJ,Sadedin S,Cowley MJ,Minoche AE,Calvo SE,Mootha VK,Ryan MT,Okazaki Y,Stroud DA,Simons C,Christodoulou J,Thorburn DR
R960-25 was used in Western Blot to investigate the ATAD3 locus in 17 subjects from 16 unrelated families with suspected mitochondrial disease, using Whole exome, whole genome and long-read DNA sequencing techniques combined with studies of RNA and quantitative proteomics.
Fri Jan 15 00:00:00 UTC 2021
Pathway-based classification of glioblastoma uncovers a mitochondrial subtype with therapeutic vulnerabilities.
Nature cancer
Garofano L,Migliozzi S,Oh YT,D'Angelo F,Najac RD,Ko A,Frangaj B,Caruso FP,Yu K,Yuan J,Zhao W,Di Stefano AL,Bielle F,Jiang T,Sims P,Suvà ML,Tang F,Su XD,Ceccarelli M,Sanson M,Lasorella A,Iavarone A
R960-25 was used in Western Blotting to study the pathway-based classification of GBM informing survival and enabling precision targeting of cancer metabolism.
Mon Feb 01 00:00:00 UTC 2021
Epigenomic tensor predicts disease subtypes and reveals constrained tumor evolution.
Cell reports
Leistico JR,Saini P,Futtner CR,Hejna M,Omura Y,Soni PN,Sandlesh P,Milad M,Wei JJ,Bulun S,Parker JB,Barish GD,Song JS,Chakravarti D
R960-25 was used in Western Blot to improve our understanding of disease states, cellular development, and differentiation, thereby facilitating future therapeutic, diagnostic, and prognostic strategies.
Tue Mar 30 00:00:00 UTC 2021
Impaired respiratory burst contributes to infections in PKCδ-deficient patients.
The Journal of experimental medicine
Neehus AL,Moriya K,Nieto-Patlán A,Le Voyer T,Lévy R,Özen A,Karakoc-Aydiner E,Baris S,Yildiran A,Altundag E,Roynard M,Haake K,Migaud M,Dorgham K,Gorochov G,Abel L,Lachmann N,Dogu F,Haskologlu S,İnce E,El-Benna J,Uzel G,Kiykim A,Boztug K,Roderick MR,Shahrooei M,Brogan PA,Abolhassani H,Hancioglu G,Parvaneh N,Belot A,Ikinciogullari A,Casanova JL,Puel A,Bustamante J
R960-25 was used in Immunoprecipitation to show that patients with PKCδ deficiency have impaired NADPH oxidase activity in various myeloid subsets, which may contribute to their CGD-like infectious phenotype.
Mon Sep 06 00:00:00 UTC 2021
Cell-penetrating Alphabody protein scaffolds for intracellular drug targeting.
Science advances
Pannecoucke E,Van Trimpont M,Desmet J,Pieters T,Reunes L,Demoen L,Vuylsteke M,Loverix S,Vandenbroucke K,Alard P,Henderikx P,Deroo S,Baatz F,Lorent E,Thiolloy S,Somers K,McGrath Y,Van Vlierberghe P,Lasters I,Savvides SN
R960-25 was used in Western Blot, Immunocytochemistry to demonstrate that the Alphabody scaffold can be engineered into a cell-penetrating protein antagonist against induced myeloid leukemia cell differentiation protein MCL-1, an intracellular target in cancer, by grafting the critical B-cell lymphoma 2 homology 3 helix of MCL-1 onto the Alphabody and tagging the scaffold's termini with designed cell-penetration polypeptides.
Mon Mar 01 00:00:00 UTC 2021
Cell-penetrating Alphabody protein scaffolds for intracellular drug targeting.
Science advances
Pannecoucke E,Van Trimpont M,Desmet J,Pieters T,Reunes L,Demoen L,Vuylsteke M,Loverix S,Vandenbroucke K,Alard P,Henderikx P,Deroo S,Baatz F,Lorent E,Thiolloy S,Somers K,McGrath Y,Van Vlierberghe P,Lasters I,Savvides SN
R960-25 was used in Western Blot, Immunocytochemistry to demonstrate that the Alphabody scaffold can be engineered into a cell-penetrating protein antagonist against induced myeloid leukemia cell differentiation protein MCL-1, an intracellular target in cancer, by grafting the critical B-cell lymphoma 2 homology 3 helix of MCL-1 onto the Alphabody and tagging the scaffold's termini with designed cell-penetration polypeptides.
Mon Mar 01 00:00:00 UTC 2021
Targeting an anchored phosphatase-deacetylase unit restores renal ciliary homeostasis.
eLife
Gopalan J,Omar MH,Roy A,Cruz NM,Falcone J,Jones KN,Forbush KA,Himmelfarb J,Freedman BS,Scott JD
R960-25 was used in Immunocytochemistry to identify the AKAP220-PPI-HDAC6 pathway as a key effector in primary cilia development.© 2021, Gopalan et al.
Mon Jul 12 00:00:00 UTC 2021
Synthetic Circuit-Driven Expression of Heterologous Enzymes for Disease Detection.
ACS synthetic biology
He J,Nissim L,Soleimany AP,Binder-Nissim A,Fleming HE,Lu TK,Bhatia SN
R960-25 was used in Immunohistochemistry to present an engineered gene circuit that, in response to cancer-associated transcriptional deregulation, expresses heterologous enzyme biomarkers whose activity can be measured by nanoparticle sensors that generate amplified detection readouts.
Fri Sep 17 00:00:00 UTC 2021
SARS-CoV-2 Nsp14 activates NF-κB signaling and induces IL-8 upregulation.
bioRxiv : the preprint server for biology
Li T,Kenney AD,Liu H,Fiches GN,Zhou D,Biswas A,Que J,Santoso N,Yount JS,Zhu J
R960-25 was used in Western Blot to identify a novel role of SARS-CoV-2 Nsp14 in causing the activation of NF-κB.
Wed May 26 00:00:00 UTC 2021
Expression of FoxP2 in the basal ganglia regulates vocal motor sequences in the adult songbird.
Nature communications
Xiao L,Merullo DP,Koch TMI,Cao M,Co M,Kulkarni A,Konopka G,Roberts TF
R960-25 was used in Western Blot to demonstrate discrete circuit origins for the dysfluent repetition of vocal elements in songbirds, with implications for speech disorders.
Tue May 11 00:00:00 UTC 2021
Expression of FoxP2 in the basal ganglia regulates vocal motor sequences in the adult songbird.
Nature communications
Xiao L,Merullo DP,Koch TMI,Cao M,Co M,Kulkarni A,Konopka G,Roberts TF
R960-25 was used in Western Blot to demonstrate discrete circuit origins for the dysfluent repetition of vocal elements in songbirds, with implications for speech disorders.
Tue May 11 00:00:00 UTC 2021
Interplay between protein acetylation and ubiquitination controls MCL1 protein stability.
Cell reports
Shimizu K,Gi M,Suzuki S,North BJ,Watahiki A,Fukumoto S,Asara JM,Tokunaga F,Wei W,Inuzuka H
R960-25 was used in Western Blotting to reveal that acetylation of MCL1 is a critical post-translational modification enhancing its oncogenic function and provide a rationale for developing innovative therapeutic strategies for MCL1-dependent tumors.
Tue Nov 09 00:00:00 UTC 2021
SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.
Theranostics
Lin L,Chen S,Wang H,Gao B,Kallakury B,Bhuvaneshwar K,Cahn K,Gusev Y,Wang X,Wu Y,Marshall JL,Zhi X,He AR
R960-25 was used in Immunoprecipitation to provide evidence that loss of SPTBN1 in HCC cells increases p65 protein stability via the inhibition of SOCS1 and enhances NF-κB activation, stimulating the release of inflammatory cytokines, which are critical molecular mechanisms for the loss of SPTBN1-induced liver cancer formation.
Thu Jul 29 00:00:00 UTC 2021
Serine residues 726 and 780 have nonredundant roles regulating STAT5a activity in luminal breast cancer.
Scientific reports
Woock AE,Grible JM,Olex AL,Harrell JC,Zot P,Idowu M,Clevenger CV
R960-25 was used in Western Blot to investigate if serine phosphorylation regulates STAT5a activity to contribute to its role in mammary oncogenesis, specifically in luminal breast cancer.
Tue Jun 29 00:00:00 UTC 2021
Protein phosphatase PPM1B inhibits DYRK1A kinase through dephosphorylation of pS258 and reduces toxic tau aggregation.
The Journal of biological chemistry
Lee YH,Im E,Hyun M,Park J,Chung KC
R960-25 was used in Western Blotting to investigate DYRK1A interactions in Down syndrome.
Tue Aug 24 00:00:00 UTC 2021
Ubiquitination-dependent and -independent repression of target genes by SETDB1 reveal a context-dependent role for its methyltransferase activity during adipogenesis.
Genes to cells : devoted to molecular & cellular mechanisms
Zhang J,Matsumura Y,Kano Y,Yoshida A,Kawamura T,Hirakawa H,Inagaki T,Tanaka T,Kimura H,Yanagi S,Fukami K,Doi T,Osborne TF,Kodama T,Aburatani H,Sakai J
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, ChIP Assay to study whether SETDB1 ubiquitination is essential for its other major role in epigenetic silencing of developmental gene programs.
Thu Jul 01 00:00:00 UTC 2021
Reduction of Derlin activity suppresses Notch-dependent tumours in the C. elegans germ line.
PLoS genetics
Singh R,Smit RB,Wang X,Wang C,Racher H,Hansen D
R960-25 was used in Immunohistochemistry to investigate if ER stress and activation of the Unfolded Protein Response may help correct for increased GLP-1/Notch signaling levels, and associated over-proliferation, in the C. elegans germline.
Wed Sep 01 00:00:00 UTC 2021
The mammalian cholesterol synthesis enzyme squalene monooxygenase is proteasomally truncated to a constitutively active form.
The Journal of biological chemistry
Coates HW,Capell-Hattam IM,Brown AJ
R960-25 was used in Western Blot to characterise a truncated squalene monooxygenase (SM) variant.
Tue Aug 31 00:00:00 UTC 2021
Elimination of Mutant mtDNA by an Optimized mpTALEN Restores Differentiation Capacities of Heteroplasmic MELAS-iPSCs.
Molecular therapy. Methods & clinical development
Yahata N,Boda H,Hata R
R960-25 was used in Western Blotting to refined a previously generated G13513A mtDNA-targeted platinum transcription activator-like effector nuclease (G13513A-mpTALEN) to more efficiently manipulate mtDNA heteroplasmy in MELAS-induced pluripotent stem cells (iPSCs).
Fri Mar 12 00:00:00 UTC 2021
PIAS1 potentiates the anti-EBV activity of SAMHD1 through SUMOylation.
Cell & bioscience
Saiada F,Zhang K,Li R
R960-25 was used in Western Blot to show that PIAS1 interacts with sterile alpha motif and HD domain 1 and promotes its SUMOylation.
Thu Jul 08 00:00:00 UTC 2021
A molecular trap inside microtubules probes luminal access by soluble proteins.
Nature chemical biology
Nihongaki Y,Matsubayashi HT,Inoue T
R960-25 was used in Immunocytochemistry-immunofluorescence to investigate microtubule luminal accessibility in living cells by an inducible protein trap inside microtubules.
Sun Aug 01 00:00:00 UTC 2021
Dynamic tracking and identification of tissue-specific secretory proteins in the circulation of live mice.
Nature communications
Kim KE,Park I,Kim J,Kang MG,Choi WG,Shin H,Kim JS,Rhee HW,Suh JM
R960-25 was used in Western Blotting to introduce a method to profile tissue-specific secretory proteins in live mice by in situ proximity labeling of ER lumen proteins through the catalytic actions of an ER-anchored TurboID.
Wed Sep 01 00:00:00 UTC 2021
CTCF orchestrates long-range cohesin-driven V(D)J recombinational scanning.
Nature
Ba Z,Lou J,Ye AY,Dai HQ,Dring EW,Lin SG,Jain S,Kyritsis N,Kieffer-Kwon KR,Casellas R,Alt FW
R960-25 was used in Western Blotting to test the potential of linear RAG scanning to mediate distal VH usage in G1-arrested v-Abl pro-B cell lines.
Thu Oct 01 00:00:00 UTC 2020
Mechanism of adrenergic CaV1.2 stimulation revealed by proximity proteomics.
Nature
Liu G,Papa A,Katchman AN,Zakharov SI,Roybal D,Hennessey JA,Kushner J,Yang L,Chen BX,Kushnir A,Dangas K,Gygi SP,Pitt GS,Colecraft HM,Ben-Johny M,Kalocsay M,Marx SO
R960-25 was used in Western Blotting to reveal an evolutionarily conserved mechanism that confers adrenergic modulation upon voltage-gated calcium channels.
Wed Jan 01 00:00:00 UTC 2020
Chemico-genetic discovery of astrocytic control of inhibition in vivo.
Nature
Takano T,Wallace JT,Baldwin KT,Purkey AM,Uezu A,Courtland JL,Soderblom EJ,Shimogori T,Maness PF,Eroglu C,Soderling SH
R960-25 was used in Immunohistochemistry to elucidate the mechanisms of astrocyte-mediated synapse formation and function.
Tue Dec 01 00:00:00 UTC 2020
Chemico-genetic discovery of astrocytic control of inhibition in vivo.
Nature
Takano T,Wallace JT,Baldwin KT,Purkey AM,Uezu A,Courtland JL,Soderblom EJ,Shimogori T,Maness PF,Eroglu C,Soderling SH
R960-25 was used in Immunohistochemistry to elucidate the mechanisms of astrocyte-mediated synapse formation and function.
Tue Dec 01 00:00:00 UTC 2020
Chemico-genetic discovery of astrocytic control of inhibition in vivo.
Nature
Takano T,Wallace JT,Baldwin KT,Purkey AM,Uezu A,Courtland JL,Soderblom EJ,Shimogori T,Maness PF,Eroglu C,Soderling SH
R960-25 was used in Immunohistochemistry to elucidate the mechanisms of astrocyte-mediated synapse formation and function.
Tue Dec 01 00:00:00 UTC 2020
Cell-Surface Proteomic Profiling in the Fly Brain Uncovers Wiring Regulators.
Cell
Li J,Han S,Li H,Udeshi ND,Svinkina T,Mani DR,Xu C,Guajardo R,Xie Q,Li T,Luginbuhl DJ,Wu B,McLaughlin CN,Xie A,Kaewsapsak P,Quake SR,Carr SA,Ting AY,Luo L
R960-25 was used in Immunocytochemistry to profile cell-surface proteomes in intact tissues.
Thu Jan 23 00:00:00 UTC 2020
The 10q26 Risk Haplotype of Age-Related Macular Degeneration Aggravates Subretinal Inflammation by Impairing Monocyte Elimination.
Immunity
Beguier F,Housset M,Roubeix C,Augustin S,Zagar Y,Nous C,Mathis T,Eandi C,Benchaboune M,Drame-Maigné A,Carpentier W,Chardonnet S,Touhami S,Blot G,Conart JB,Charles-Messance H,Potey A,Girmens JF,Paques M,Blond F,Leveillard T,Koertvely E,Roger JE,Sahel JA,Sapieha P,Delarasse C,Guillonneau X,Sennlaub F
R960-25 was used in Proximity Ligation Assay (PLA) to study the mechanisms whereby the risk 10q26 haplotype promotes age-related macular degeneration.
Tue Aug 18 00:00:00 UTC 2020
Chromatin binding of FOXA1 is promoted by LSD1-mediated demethylation in prostate cancer.
Nature genetics
Gao S,Chen S,Han D,Wang Z,Li M,Han W,Besschetnova A,Liu M,Zhou F,Barrett D,Luong MP,Owiredu J,Liang Y,Ahmed M,Petricca J,Patalano S,Macoska JA,Corey E,Chen S,Balk SP,He HH,Cai C
R960-25 was used in Chromatin immunoprecipitation to show, in prostate cancer cells, that LSD1 associates with FOXA1 and active enhancer markers, and that LSD1 inhibition globally disrupts FOXA1 chromatin binding.
Thu Oct 01 00:00:00 UTC 2020
Fructose-1,6-Bisphosphatase 2 Inhibits Sarcoma Progression by Restraining Mitochondrial Biogenesis.
Cell metabolism
Huangyang P,Li F,Lee P,Nissim I,Weljie AM,Mancuso A,Li B,Keith B,Yoon SS,Simon MC
R960-25 was used in Chromatin immunoprecipitation to show that expression of the gluconeogenic isozyme fructose-1,6-bisphosphatase 2 (FBP2) is silenced in a broad spectrum of sarcoma subtypes, revealing an apparent common metabolic feature shared by diverse STSs.
Tue Jan 07 00:00:00 UTC 2020
lncRNA Sensing of a Viral Suppressor of RNAi Activates Non-canonical Innate Immune Signaling in Drosophila.
Cell host & microbe
Zhang L,Xu W,Gao X,Li W,Qi S,Guo D,Ajayi OE,Ding SW,Wu Q
R960-25 was used in RNA Immunoprecipitation, Immunoprecipitation to report the identification of VINR, a Drosophila VSR-interacting long non-coding (lnc) RNA that activates non-canonical innate immune signaling upon detection of the dsRNA-binding VSR of Drosophila C virus (DCV).
Wed Jan 08 00:00:00 UTC 2020
lncRNA Sensing of a Viral Suppressor of RNAi Activates Non-canonical Innate Immune Signaling in Drosophila.
Cell host & microbe
Zhang L,Xu W,Gao X,Li W,Qi S,Guo D,Ajayi OE,Ding SW,Wu Q
R960-25 was used in RNA Immunoprecipitation, Immunoprecipitation to report the identification of VINR, a Drosophila VSR-interacting long non-coding (lnc) RNA that activates non-canonical innate immune signaling upon detection of the dsRNA-binding VSR of Drosophila C virus (DCV).
Wed Jan 08 00:00:00 UTC 2020
Ancient familial Mediterranean fever mutations in human pyrin and resistance to Yersinia pestis.
Nature immunology
Park YH,Remmers EF,Lee W,Ombrello AK,Chung LK,Shilei Z,Stone DL,Ivanov MI,Loeven NA,Barron KS,Hoffmann P,Nehrebecky M,Akkaya-Ulum YZ,Sag E,Balci-Peynircioglu B,Aksentijevich I,Gül A,Rotimi CN,Chen H,Bliska JB,Ozen S,Kastner DL,Shriner D,Chae JJ
R960-25 was used in Western Blot to demonstrate the selective advantage of MEFV mutations for Yersinia Pestis resistance.
Sat Aug 01 00:00:00 UTC 2020
Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription.
Cell stem cell
Li Q,Sun Y,Jarugumilli GK,Liu S,Dang K,Cotton JL,Xiol J,Chan PY,DeRan M,Ma L,Li R,Zhu LJ,Li JH,Leiter AB,Ip YT,Camargo FD,Luo X,Johnson RL,Wu X,Mao J
R960-25 was used in Immunoprecipitation to demonstrate if Lats1/2, the core Hippo kinases, are essential to maintain Wnt pathway activity and intestinal stem cells.
Thu May 07 00:00:00 UTC 2020
The innate immune effector ISG12a promotes cancer immunity by suppressing the canonical Wnt/β-catenin signaling pathway.
Cellular & molecular immunology
Deng R,Zuo C,Li Y,Xue B,Xun Z,Guo Y,Wang X,Xu Y,Tian R,Chen S,Liu Q,Chen J,Wang J,Huang X,Li H,Guo M,Wang X,Yang M,Wu Z,Wang J,Ma J,Hu J,Li G,Tang S,Tu Z,Ji H,Zhu H
R960-25 was used in Western Blotting to propose that inhibition of canonical Wnt/β-catenin signalling by ISG12a, an innate immune effector, can promote cancer immunity.
Sun Nov 01 00:00:00 UTC 2020
BANK1 interacts with TRAF6 and MyD88 in innate immune signaling in B cells.
Cellular & molecular immunology
Georg I,Díaz-Barreiro A,Morell M,Pey AL,Alarcón-Riquelme ME
R960-25 was used in Western Blotting to investigate the interaction of BANK1 with two key mediators in interferon and inflammatory cytokine production, TRAF6 and MyD88.
Tue Sep 01 00:00:00 UTC 2020
H3K27me3-mediated PGC1α gene silencing promotes melanoma invasion through WNT5A and YAP.
The Journal of clinical investigation
Luo C,Balsa E,Perry EA,Liang J,Tavares CD,Vazquez F,Widlund HR,Puigserver P
R960-25 was used in Immunoprecipitation to show that PGC1α expression in melanoma cells was silenced by chromatin modifications that involve promoter H3K27 trimethylation.
Mon Feb 03 00:00:00 UTC 2020
Context-Dependent Gene Regulation by Homeodomain Transcription Factor Complexes Revealed by Shape-Readout Deficient Proteins.
Molecular cell
Kribelbauer JF,Loker RE,Feng S,Rastogi C,Abe N,Rube HT,Bussemaker HJ,Mann RS
R960-25 was used in Immunohistochemistry to demonstrate that high-throughput in vitro DNA binding assays coupled with unbiased computational analysis provide unprecedented insight into how different DNA sequences select distinct compositions and configurations of homeodomain TF complexes.
Thu Apr 02 00:00:00 UTC 2020
PRIMPOL-Mediated Adaptive Response Suppresses Replication Fork Reversal in BRCA-Deficient Cells.
Molecular cell
Quinet A,Tirman S,Jackson J,Šviković S,Lemaçon D,Carvajal-Maldonado D,González-Acosta D,Vessoni AT,Cybulla E,Wood M,Tavis S,Batista LFZ,Méndez J,Sale JE,Vindigni A
R960-25 was used in Western Blotting to demonstrate cisplatin-exposed BRCA1-deficient cancer cells do not show detectable fork degradation.
Thu Feb 06 00:00:00 UTC 2020
A Multibasic Cleavage Site in the Spike Protein of SARS-CoV-2 Is Essential for Infection of Human Lung Cells.
Molecular cell
Hoffmann M,Kleine-Weber H,Pöhlmann S
R960-25 was used in Western Blotting to report that the cellular protease furin cleaves the spike protein at the S1/S2 site and that cleavage is essential for S-protein-mediated cell-cell fusion and entry into human lung cells.
Thu May 21 00:00:00 UTC 2020
Kinase Interaction Network Expands Functional and Disease Roles of Human Kinases.
Molecular cell
Buljan M,Ciuffa R,van Drogen A,Vichalkovski A,Mehnert M,Rosenberger G,Lee S,Varjosalo M,Pernas LE,Spegg V,Snijder B,Aebersold R,Gstaiger M
R960-25 was used in Western Blotting to investigate the human kinase interaction network using mass spectrometry.
Thu Aug 06 00:00:00 UTC 2020
DNAJB6, a Key Factor in Neuronal Sensitivity to Amyloidogenesis.
Molecular cell
Thiruvalluvan A,de Mattos EP,Brunsting JF,Bakels R,Serlidaki D,Barazzuol L,Conforti P,Fatima A,Koyuncu S,Cattaneo E,Vilchez D,Bergink S,Boddeke EHWG,Copray S,Kampinga HH
R960-25 was used in Flow cytometry/Cell sorting to show that stem cells are intrinsically protected against amyloidogenesis and protein aggregates are dominantly prese in vivo.nt in neurons due to loss of DNAJB6 expression upon differentiation.
Thu Apr 16 00:00:00 UTC 2020
The conserved elongation factor Spn1 is required for normal transcription, histone modifications, and splicing in Saccharomyces cerevisiae.
Nucleic acids research
Reim NI,Chuang J,Jain D,Alver BH,Park PJ,Winston F
R960-25 was used in Western Blot, ChIP assay to show that Spn1 affects multiple aspects of gene expression and provide additional evidence that it functions as a histone chaperone in vivo.
Fri Oct 09 00:00:00 UTC 2020
The conserved elongation factor Spn1 is required for normal transcription, histone modifications, and splicing in Saccharomyces cerevisiae.
Nucleic acids research
Reim NI,Chuang J,Jain D,Alver BH,Park PJ,Winston F
R960-25 was used in Western Blot, ChIP assay to show that Spn1 affects multiple aspects of gene expression and provide additional evidence that it functions as a histone chaperone in vivo.
Fri Oct 09 00:00:00 UTC 2020
Antibody Therapy Targeting RAN Proteins Rescues C9 ALS/FTD Phenotypes in C9orf72 Mouse Model.
Neuron
Nguyen L,Montrasio F,Pattamatta A,Tusi SK,Bardhi O,Meyer KD,Hayes L,Nakamura K,Banez-Coronel M,Coyne A,Guo S,Laboissonniere LA,Gu Y,Narayanan S,Smith B,Nitsch RM,Kankel MW,Rushe M,Rothstein J,Zu T,Grimm J,Ranum LPW
R960-25 was used in Western Blotting to demonstrate that RAN proteins drive C9-ALS/FTD in C9-BAC transgenic mice and establish a novel therapeutic approach for C9orf72 ALS/FTD and other RAN-protein diseases.
Wed Feb 19 00:00:00 UTC 2020
Ankyrin Is An Intracellular Tether for TMC Mechanotransduction Channels.
Neuron
Tang YQ,Lee SA,Rahman M,Vanapalli SA,Lu H,Schafer WR
R960-25 was used in Western Blot to identify UNC-44/ankyrin as an essential component of the TMC-1 mechanotransduction channel complex in the sensory cilia of Caenorhabditis elegans mechanoreceptor neurons.
Wed Jul 08 00:00:00 UTC 2020
LRRTMs Organize Synapses through Differential Engagement of Neurexin and PTPσ.
Neuron
Roppongi RT,Dhume SH,Padmanabhan N,Silwal P,Zahra N,Karimi B,Bomkamp C,Patil CS,Champagne-Jorgensen K,Twilley RE,Zhang P,Jackson MF,Siddiqui TJ
R960-25 was used in Western Blotting to show that leucine-rich-repeat transmembrane neuronal proteins (LRRTMs) differentially engage the protein domains of Nrx but require its heparan sulfate (HS) modification to induce presynaptic differentiation.
Wed Apr 08 00:00:00 UTC 2020
Sensing of endogenous nucleic acids by ZBP1 induces keratinocyte necroptosis and skin inflammation.
The Journal of experimental medicine
Devos M,Tanghe G,Gilbert B,Dierick E,Verheirstraeten M,Nemegeer J,de Reuver R,Lefebvre S,De Munck J,Rehwinkel J,Vandenabeele P,Declercq W,Maelfait J
R960-25 was used in Immunoprecipitation to show that sensing of endogenous nucleic acids by ZBP1 is critical in driving skin pathology characterised by antiviral and IL-17 immune responses.
Mon Jul 06 00:00:00 UTC 2020
LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma.
EMBO molecular medicine
Leiendecker L,Jung PS,Krecioch I,Neumann T,Schleiffer A,Mechtler K,Wiesner T,Obenauf AC
R960-25 was used in Western Blotting to target epigenetic regulators in a pharmacological screen and discover that the lysine-specific histone demethylase 1A (LSD1/KDM1A) is required for Merkel cell carcinoma growth in vitro and in vivo.
Fri Nov 06 00:00:00 UTC 2020
Linc-MYH configures INO80 to regulate muscle stem cell numbers and skeletal muscle hypertrophy.
The EMBO journal
Schutt C,Hallmann A,Hachim S,Klockner I,Valussi M,Atzberger A,Graumann J,Braun T,Boettger T
R960-25 was used in Western Blotting to demonstrate that the evolutionary conserved long noncoding RNA linc-MYH plays a major role in the control of MuSC proliferation by specifically regulating composition and activity of the INO80 complex.
Mon Nov 16 00:00:00 UTC 2020
Intracellular Role for the Matrix-Modifying Enzyme Lox in Regulating Transcription Factor Subcellular Localization and Activity in Muscle Regeneration.
Developmental cell
Gabay Yehezkely R,Zaffryar-Eilot S,Kaganovsky A,Fainshtain Malka N,Aviram R,Livneh I,Hasson P
R960-25 was used in Immunoprecipitation to demonstrate that lysyl oxidase (Lox), known for its ECM-modifying activities, primarily collagen crosslinking, also directly regulates transcription factor (TF) localization.
Mon May 18 00:00:00 UTC 2020
Intracellular Role for the Matrix-Modifying Enzyme Lox in Regulating Transcription Factor Subcellular Localization and Activity in Muscle Regeneration.
Developmental cell
Gabay Yehezkely R,Zaffryar-Eilot S,Kaganovsky A,Fainshtain Malka N,Aviram R,Livneh I,Hasson P
R960-25 was used in Immunoprecipitation to demonstrate that lysyl oxidase (Lox), known for its ECM-modifying activities, primarily collagen crosslinking, also directly regulates transcription factor (TF) localization.
Mon May 18 00:00:00 UTC 2020
Reprogramming Axial Level Identity to Rescue Neural-Crest-Related Congenital Heart Defects.
Developmental cell
Gandhi S,Ezin M,Bronner ME
R960-25 was used in Immunohistochemistry to reveal a transcriptional program sufficient to confer cardiac potential onto trunk neural crest cells, thus implicating new genes in cardiovascular birth defects.
Mon May 04 00:00:00 UTC 2020
CD44 Promotes PD-L1 Expression and Its Tumor-Intrinsic Function in Breast and Lung Cancers.
Cancer research
Kong T,Ahn R,Yang K,Zhu X,Fu Z,Morin G,Bramley R,Cliffe NC,Xue Y,Kuasne H,Li Q,Jung S,Gonzalez AV,Camilleri-Broet S,Guiot MC,Park M,Ursini-Siegel J,Huang S
R960-25 was used in Immunoprecipitation to provide a novel basis for CD44 as a critical therapeutic target to suppress PD-L1 tumor-intrinsic function.
Sat Feb 01 00:00:00 UTC 2020
UBR5 Is Coamplified with MYC in Breast Tumors and Encodes an Ubiquitin Ligase That Limits MYC-Dependent Apoptosis.
Cancer research
Qiao X,Liu Y,Prada ML,Mohan AK,Gupta A,Jaiswal A,Sharma M,Merisaari J,Haikala HM,Talvinen K,Yetukuri L,Pylvänäinen JW,Klefström J,Kronqvist P,Meinander A,Aittokallio T,Hietakangas V,Eilers M,Westermarck J
R960-25 was used in Western Blotting to identify UBR5 as a novel MYC regulator, the inactivation of which could be very important for understanding of MYC dysregulation on cancer cells.
Wed Apr 01 00:00:00 UTC 2020
The Pathognomonic FOXL2 C134W Mutation Alters DNA-Binding Specificity.
Cancer research
Carles A,Trigo-Gonzalez G,Cao Q,Cheng SG,Moksa M,Bilenky M,Huntsman DG,Morin GB,Hirst M
R960CUS was used in Immunoprecipitation to explore the mechanisms of the FOXL2 C134W mutation to the pathogenesis of adult-type granulosa cell tumours.
Tue Sep 01 00:00:00 UTC 2020
The Pathognomonic FOXL2 C134W Mutation Alters DNA-Binding Specificity.
Cancer research
Carles A,Trigo-Gonzalez G,Cao Q,Cheng SG,Moksa M,Bilenky M,Huntsman DG,Morin GB,Hirst M
R960CUS was used in Immunoprecipitation to explore the mechanisms of the FOXL2 C134W mutation to the pathogenesis of adult-type granulosa cell tumours.
Tue Sep 01 00:00:00 UTC 2020
Aurora B-dependent Ndc80 degradation regulates kinetochore composition in meiosis.
Genes & development
Chen J,Liao A,Powers EN,Liao H,Kohlstaedt LA,Evans R,Holly RM,Kim JK,Jovanovic M,Ünal E
R960-25 was used in Western Blot to elucidate the mechanism by which meiotic cells modulate their kinetochore composition through regulated Ndc80 degradation, and demonstrate that Aurora B-dependent regulation of kinetochores extends beyond altering microtubule attachments.
Sat Feb 01 00:00:00 UTC 2020
USP7 regulates ALS-associated proteotoxicity and quality control through the NEDD4L-SMAD pathway.
Proceedings of the National Academy of Sciences of the United States of America
Zhang T,Periz G,Lu YN,Wang J
R960-25 was used in Western Blot, Immunoprecipitation to report the identification of a ubiquitin-specific protease, USP7, as a regulatory switch in a protein quality-control system that defends against proteotoxicity.
Tue Nov 10 00:00:00 UTC 2020
USP7 regulates ALS-associated proteotoxicity and quality control through the NEDD4L-SMAD pathway.
Proceedings of the National Academy of Sciences of the United States of America
Zhang T,Periz G,Lu YN,Wang J
R960-25 was used in Western Blot, Immunoprecipitation to report the identification of a ubiquitin-specific protease, USP7, as a regulatory switch in a protein quality-control system that defends against proteotoxicity.
Tue Nov 10 00:00:00 UTC 2020
Molecular characterization of a fungal gasdermin-like protein.
Proceedings of the National Academy of Sciences of the United States of America
Daskalov A,Mitchell PS,Sandstrom A,Vance RE,Glass NL
R960-25 was used in Western Blotting to investigate the molecular bases of rcd-1-dependent cell death.
Tue Aug 04 00:00:00 UTC 2020
14-3-3ζ-TRAF5 axis governs interleukin-17A signaling.
Proceedings of the National Academy of Sciences of the United States of America
McGowan J,Peter C,Kim J,Popli S,Veerman B,Saul-McBeth J,Conti H,Pruett-Miller SM,Chattopadhyay S,Chakravarti R
R960-25 was used in Immunocytochemistry to study how essential 14-3-3ζ is for IL-17 signaling by differentially regulating the signal-induced IL-6 and CXCL-1.
Tue Oct 06 00:00:00 UTC 2020
Chchd2 regulates mitochondrial morphology by modulating the levels of Opa1.
Cell death and differentiation
Liu W,Duan X,Xu L,Shang W,Zhao J,Wang L,Li JC,Chen CH,Liu JP,Tong C
R960-25 was used in Western Blot to elucidate the role of the Drosophila Chchd2 gene in mitochondrial morphology and tissue homeostasis.
Mon Jun 01 00:00:00 UTC 2020
Dynein light chain binding determines complex formation and posttranslational stability of the Bcl-2 family members Bmf and Bim.
Cell death and differentiation
Singh PK,Roukounakis A,Weber A,Das KK,Sohm B,Villunger A,Garcia-Saez AJ,Häcker G
R960-25 was used in Western Blotting to find that Bim and Bmf interact with both isoforms of DYNLL (DYNLL1 and DYNLL2).
Sat Feb 01 00:00:00 UTC 2020
Mutant GTF2I induces cell transformation and metabolic alterations in thymic epithelial cells.
Cell death and differentiation
Kim IK,Rao G,Zhao X,Fan R,Avantaggiati ML,Wang Y,Zhang YW,Giaccone G
R960-25 was used in Immunohistochemistry to investigate the pathogenesis of thymic epithelial tumors (TETs).
Wed Jul 01 00:00:00 UTC 2020
Gain-of-Function MN1 Truncation Variants Cause a Recognizable Syndrome with Craniofacial and Brain Abnormalities.
American journal of human genetics
Miyake N,Takahashi H,Nakamura K,Isidor B,Hiraki Y,Koshimizu E,Shiina M,Sasaki K,Suzuki H,Abe R,Kimura Y,Akiyama T,Tomizawa SI,Hirose T,Hamanaka K,Miyatake S,Mitsuhashi S,Mizuguchi T,Takata A,Obo K,Kato M,Ogata K,Matsumoto N
R960-25 was used in Western Blotting to report on three individuals who have de novo MN1 variants that lead to a protein lacking the carboxyl (C) terminus and who presented with severe developmental delay, craniofacial abnormalities with specific facial features, and structural abnormalities in the brain.
Thu Jan 02 00:00:00 UTC 2020
A Cycle of Ubiquitination Regulates Adaptor Function of the Nedd4-Family Ubiquitin Ligase Rsp5.
Current biology : CB
MacDonald C,Shields SB,Williams CA,Winistorfer S,Piper RC
R960-25 was used in Western Blotting to support a model whereby ubiquitinated Rsp5 adaptors are more active when "locked" onto Rsp5 via its N-lobe ubiquitin-binding surface and less active when they are "unlocked" by Ubp2-mediated deubiquitination.
Mon Feb 03 00:00:00 UTC 2020
Generation of a reporter yellow fever virus for high throughput antiviral assays.
Antiviral research
Sanchez-Velazquez R,de Lorenzo G,Tandavanitj R,Setthapramote C,Bredenbeek PJ,Bozzacco L,MacDonald MR,Clark JJ,Rice CM,Patel AH,Kohl A,Varjak M
R960-25 was used in Western Blotting to validate our assay by using a range of inhibitors including drugs, immune sera and neutralizing single chain variable fragments (scFv). In light of the recent upsurge in YFV and a potential spread of the virus, this assay is a further tool in the development of antiviral therapy against YFV.
Sun Nov 01 00:00:00 UTC 2020
Plasmin improves blood-gas barrier function in oedematous lungs by cleaving epithelial sodium channels.
British journal of pharmacology
Zhao R,Ali G,Nie HG,Chang Y,Bhattarai D,Su X,Zhao X,Matthay MA,Ji HL
R960-25 was used in Western Blot to test whether plasmin cleaves epithelial sodium channels (ENaC) to resolve lung oedema fluid.
Wed Jul 01 00:00:00 UTC 2020
Influenza A viruses limit NLRP3-NEK7-complex formation and pyroptosis in human macrophages.
EMBO reports
Boal-Carvalho I,Mazel-Sanchez B,Silva F,Garnier L,Yildiz S,Bonifacio JP,Niu C,Williams N,Francois P,Schwerk N,Schöning J,Carlens J,Viemann D,Hugues S,Schmolke M
R960-25 was used in Western Blot to show that interaction of a recently identified licensing kinase NEK7 with NLRP3 is diminished, which is required to initiate inflammasome assembly.
Thu Dec 03 00:00:00 UTC 2020
TRIM21-mediated proteasomal degradation of SAMHD1 regulates its antiviral activity.
EMBO reports
Li Z,Huan C,Wang H,Liu Y,Liu X,Su X,Yu J,Zhao Z,Yu XF,Zheng B,Zhang W
R960-25 was used in Western Blotting to show the antiviral activity of SAMHD1 is regulated by proteasomal degradation activated by E3 ubiquitin ligase TRIM21.
Tue Jan 07 00:00:00 UTC 2020
Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy.
EMBO reports
Martello A,Lauriola A,Mellis D,Parish E,Dawson JC,Imrie L,Vidmar M,Gammoh N,Mitić T,Brittan M,Mills NL,Carragher NO,D'Arca D,Caporali A
R960-25 was used in Western Blotting to reveal that TCHP regulates endothelial cell function via an autophagy-mediated mechanism.
Fri Jul 03 00:00:00 UTC 2020
Loss-of-function variants in C3ORF52 result in localized autosomal recessive hypotrichosis.
Genetics in medicine : official journal of the American College of Medical Genetics
Malki L,Sarig O,Cesarato N,Mohamad J,Canter T,Assaf S,Pavlovsky M,Vodo D,Anis Y,Bihari O,Malovitski K,Gat A,Thiele H,White BEP,Samuelov L,Nanda A,Paller AS,Betz RC,Sprecher E
R960-25 was used in Western Blot to investigate the genetic basis of localized autosomal recessive hypotrichosis in patients without pathogenic variants in the DSG4, LIPH, LPAR6 genes.
Wed Jul 01 00:00:00 UTC 2020
NF-κB disinhibition contributes to dendrite defects in fly models of neurodegenerative diseases.
The Journal of cell biology
Han MH,Kwon MJ,Ko BS,Hyeon DY,Lee D,Kim HJ,Hwang D,Lee SB
R960-25 was used in Immunohistochemistry to propose disinhibition of latent toxicity of Relish/NF-κB as a novel pathogenic mechanism underlying dendrite pathology in NDs.
Mon Dec 07 00:00:00 UTC 2020
NF-κB disinhibition contributes to dendrite defects in fly models of neurodegenerative diseases.
The Journal of cell biology
Han MH,Kwon MJ,Ko BS,Hyeon DY,Lee D,Kim HJ,Hwang D,Lee SB
R960-25 was used in Immunohistochemistry to propose disinhibition of latent toxicity of Relish/NF-κB as a novel pathogenic mechanism underlying dendrite pathology in NDs.
Mon Dec 07 00:00:00 UTC 2020
Deorphanizing FAM19A proteins as pan-neurexin ligands with an unusual biosynthetic binding mechanism.
The Journal of cell biology
Khalaj AJ,Sterky FH,Sclip A,Schwenk J,Brunger AT,Fakler B,Südhof TC
R960-25 was used in Western Blot, Immunocytochemistry to identify FAM19A/TAFA proteins, "orphan" cytokines, as neurexin regulators that interact with all neurexins, except for neurexin-1γ, via an unusual mechanism.
Mon Sep 07 00:00:00 UTC 2020
Deorphanizing FAM19A proteins as pan-neurexin ligands with an unusual biosynthetic binding mechanism.
The Journal of cell biology
Khalaj AJ,Sterky FH,Sclip A,Schwenk J,Brunger AT,Fakler B,Südhof TC
R960-25 was used in Western Blot, Immunocytochemistry to identify FAM19A/TAFA proteins, "orphan" cytokines, as neurexin regulators that interact with all neurexins, except for neurexin-1γ, via an unusual mechanism.
Mon Sep 07 00:00:00 UTC 2020
A molecular mechanism for the procentriole recruitment of Ana2.
The Journal of cell biology
McLamarrah TA,Speed SK,Ryniawec JM,Buster DW,Fagerstrom CJ,Galletta BJ,Rusan NM,Rogers GC
R960-25 was used in Western Blot, Immunoprecipitation to show that binding of Ana2 to the Sas4 G-box enables hyperphosphorylation of the Ana2 N terminus by Plk4.
Mon Feb 03 00:00:00 UTC 2020
A molecular mechanism for the procentriole recruitment of Ana2.
The Journal of cell biology
McLamarrah TA,Speed SK,Ryniawec JM,Buster DW,Fagerstrom CJ,Galletta BJ,Rusan NM,Rogers GC
R960-25 was used in Western Blot, Immunoprecipitation to show that binding of Ana2 to the Sas4 G-box enables hyperphosphorylation of the Ana2 N terminus by Plk4.
Mon Feb 03 00:00:00 UTC 2020
Developing Broad-Spectrum Antivirals Using Porcine and Rhesus Macaque Models.
The Journal of infectious diseases
Qiu X,Taft J,Bogunovic D
R960-25 was used in Western Blotting to identify animal models that recapitulate the human condition for the development of ISG15-targeting broad-spectrum antivirals.
Mon Mar 02 00:00:00 UTC 2020
Phosphoinositide-binding proteins mark, shape and functionally modulate highly-diverged endocytic compartments in the parasitic protist Giardia lamblia.
PLoS pathogens
Cernikova L,Faso C,Hehl AB
R960-25 was used in Immunocytochemistry to indicate that the Giardia lineage presents a strikingly divergent clathrin-centered network.
Sat Feb 01 00:00:00 UTC 2020
Profiling of immune related genes silenced in EBV-positive gastric carcinoma identified novel restriction factors of human gammaherpesviruses.
PLoS pathogens
Fiches GN,Zhou D,Kong W,Biswas A,Ahmed EH,Baiocchi RA,Zhu J,Santoso N
R960-25 was used in Chromatin immunoprecipitation to identify that immune related genes (IRGs) is a group of host genes preferentially silenced in EBV-positive gastric cancers through DNA hypermethylation.
Sat Aug 01 00:00:00 UTC 2020
Hyd ubiquitinates the NF-κB co-factor Akirin to operate an effective immune response in Drosophila.
PLoS pathogens
Cammarata-Mouchtouris A,Nguyen XH,Acker A,Bonnay F,Goto A,Orian A,Fauvarque MO,Boutros M,Reichhart JM,Matt N
R960-25 was used in Western Blotting to link the action of an E3 ubiquitin ligase to the activation of immune effector genes, deepening our understanding of the involvement of ubiquitination in inflammation and identifying a potential target for the control of inflammatory diseases.
Wed Apr 01 00:00:00 UTC 2020
Mis12 controls cyclin B1 stabilization via Cdc14B-mediated APC/CCdh1 regulation during meiotic G2/M transition in mouse oocytes.
Development (Cambridge, England)
Bai GY,Choe MH,Kim JS,Oh JS
R960-25 was used in Immunocytochemistry to demonstrate that Mis12 is required for meiotic G2/M transition but is dispensable for meiotic progression through meiosis I and II.
Mon Apr 27 00:00:00 UTC 2020
The transcription factor E2A drives neural differentiation in pluripotent cells.
Development (Cambridge, England)
Rao C,Malaguti M,Mason JO,Lowell S
R960-25 was used in Immunocytochemistry to demonstrate that overexpression of a forced E2A homodimer is sufficient to drive robust neural commitment in pluripotent cells, even under non-permissive conditions.
Mon Jun 22 00:00:00 UTC 2020
The transcription factor E2A drives neural differentiation in pluripotent cells.
Development (Cambridge, England)
Rao C,Malaguti M,Mason JO,Lowell S
R960-25 was used in Immunocytochemistry to demonstrate that overexpression of a forced E2A homodimer is sufficient to drive robust neural commitment in pluripotent cells, even under non-permissive conditions.
Mon Jun 22 00:00:00 UTC 2020
Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Suppresses Type I and Type III Interferon Induction by Targeting RIG-I Signaling.
Journal of virology
Chang CY,Liu HM,Chang MF,Chang SC
R960-25 was used in Western Blotting to reveal how MERS-CoV evades innate immunity and provide insights into the interplay between host immune response and viral pathogenicity.
Tue Jun 16 00:00:00 UTC 2020
Relative Contributions of the cGAS-STING and TLR3 Signaling Pathways to Attenuation of Herpes Simplex Virus 1 Replication.
Journal of virology
Latif MB,Raja R,Kessler PM,Sen GC
R960-25 was used in Western Blot to elucidate the role of the cGAS-STING and TLR3 pathways in the attenuation of viral infection.
Fri Feb 28 00:00:00 UTC 2020
BPIFB3 Regulates Endoplasmic Reticulum Morphology To Facilitate Flavivirus Replication.
Journal of virology
Evans AS,Lennemann NJ,Coyne CB
R960-25 was used in Immunocytochemistry-Immunofluorescence to show the importance of the host factor BPI fold-containing family B member 3(BPIFB3) in regulating flavivirus replication and the reticulophagy regulator 1 (RETREG1)-dependent reticulophagy pathway is antiviral to both dengue virus(DENV) and Zika virus(ZIKV).
Thu Apr 16 00:00:00 UTC 2020
Upregulation of adipocyte enhancer-binding protein 1 in endothelial cells promotes tumor angiogenesis in colorectal cancer.
Cancer science
Yorozu A,Yamamoto E,Niinuma T,Tsuyada A,Maruyama R,Kitajima H,Numata Y,Kai M,Sudo G,Kubo T,Nishidate T,Okita K,Takemasa I,Nakase H,Sugai T,Takano K,Suzuki H
R960-25 was used in Western Blotting to study tumour angiogenesis as a therapeutic target in colorectal cancer (CRC).
Fri May 01 00:00:00 UTC 2020
Discovery and characterization of a neutralizing pan-ELR+CXC chemokine monoclonal antibody.
mAbs
Boyles JS,Beidler CB,Strifler BA,Girard DS,Druzina Z,Durbin JD,Swearingen ML,Lee LN,Kikly K,Chintharlapalli S,Witcher DR
R960CUS was used in Flow cytometry/Cell sorting to provide a robust preclinical characterization of LY3041658 for which the efficacy and safety is being evaluated in human clinical trials for neutrophilic skin diseases.
Mon Sep 20 00:00:00 UTC 2021
Genome-wide CRISPR screen identifies ZIC2 as an essential gene that controls the cell fate of early mesodermal precursors to human heart progenitors.
Stem cells (Dayton, Ohio)
Xu J,Zhou C,Foo KS,Yang R,Xiao Y,Bylund K,Sahara M,Chien KR
R960-25 was used in Immunocytochemistry-immunoflourescence to determine the key regulators for cardiac progenitor formation, we have developed a two-stage genome-wide CRISPR-knockout screen.
Mon Jun 01 00:00:00 UTC 2020
Toll interacting protein protects bronchial epithelial cells from bleomycin-induced apoptosis.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Li X,Kim SE,Chen TY,Wang J,Yang X,Tabib T,Tan J,Guo B,Fung S,Zhao J,Sembrat J,Rojas M,Shiva S,Lafyatis R,St Croix C,Alder JK,Di YP,Kass DJ,Zhang Y
R960-25 was used in Western Blot to conclude that global downregulation of the TOLLIP gene in IPF lungs may predispose injured lung epithelial cells to apoptosis and to the development of IPF.
Sat Aug 01 00:00:00 UTC 2020
IQGAP1 restrains T-cell cosignaling mediated by OX40.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Okuyama Y,Nagashima H,Ushio-Fukai M,Croft M,Ishii N,So T
R960-25 was used in Western Blot to define an important component of the OX40 cosignaling that restricts inflammation driven by antigen-activated T cells.
Wed Jan 01 00:00:00 UTC 2020
Drosophila P75 safeguards oogenesis by preventing H3K9me2 spreading.
Journal of genetics and genomics = Yi chuan xue bao
Dou K,Liu Y,Zhang Y,Wang C,Huang Y,Zhang ZZ
R960-25 was used in Immunohistochemistry to characterise the molecular function of dP75, the Drosophila homolog of LEDGF/p75, during oogenesis.
Mon Apr 20 00:00:00 UTC 2020
Drosophila P75 safeguards oogenesis by preventing H3K9me2 spreading.
Journal of genetics and genomics = Yi chuan xue bao
Dou K,Liu Y,Zhang Y,Wang C,Huang Y,Zhang ZZ
R960-25 was used in Immunohistochemistry to characterise the molecular function of dP75, the Drosophila homolog of LEDGF/p75, during oogenesis.
Mon Apr 20 00:00:00 UTC 2020
Drosophila Prp40 localizes to the histone locus body and regulates gene transcription and development.
Journal of cell science
Prieto-Sánchez S,Moreno-Castro C,Hernández-Munain C,Suñé C
R960-25 was used in Immunohistochemistry (PFA fixed) to identify the Drosophila Prp40 pre-mRNA processing factor (dPrp40, annotated as CG3542) as a novel histone locus body component.
Tue Apr 07 00:00:00 UTC 2020
Phosphorylation and dephosphorylation of Ser852 and Ser889 control the clustering, localization and function of PAR3.
Journal of cell science
Yamashita K,Mizuno K,Furukawa K,Hirose H,Sakurai N,Masuda-Hirata M,Amano Y,Hirose T,Suzuki A,Ohno S
R960-25 was used in Immunocytochemistry to demonstrate that unphosphorylatable forms of PAR3 exhibited a low molecular turnover and failed to coordinate rapid reconstruction of the tight junction, supporting that both the phosphorylated and dephosphorylated states are essential for the functional integrity of PAR3.
Mon Nov 30 00:00:00 UTC 2020
The mammalian cytosolic thioredoxin reductase pathway acts via a membrane protein to reduce ER-localised proteins.
Journal of cell science
Cao X,Lilla S,Cao Z,Pringle MA,Oka OBV,Robinson PJ,Szmaja T,van Lith M,Zanivan S,Bulleid NJ
R960-25 was used in Western Blotting to provide compelling evidence for the crucial role of the cytosol in regulating ER redox homeostasis, ensuring correct protein folding and facilitating the degradation of misfolded ER proteins.
Thu Apr 30 00:00:00 UTC 2020
Crumbs, Galla and Xpd are required for Kinesin-5 regulation in mitosis and organ growth in Drosophila.
Journal of cell science
Hwang JH,Vuong LT,Choi KW
R960-25 was used in Western Blotting to determine the molecular regulators of Kinesin-5-dependent mitosis and organ growth in Drosophila.
Tue Jun 23 00:00:00 UTC 2020
Conditional switching of KIF2A mutation provides new insights into cortical malformation pathogeny.
Human molecular genetics
Gilet JG,Ivanova EL,Trofimova D,Rudolf G,Meziane H,Broix L,Drouot N,Courraud J,Skory V,Voulleminot P,Osipenko M,Bahi-Buisson N,Yalcin B,Birling MC,Hinckelmann MV,Kwok BH,Allingham JS,Chelly J
R960-25 was used in Immunohistochemistry to investigate RosaCre-, NestinCre- and NexCre-driven expression of the mutation to dissect the pathophysiological mechanisms underlying neurodevelopmental cortical abnormalities.
Fri Mar 27 00:00:00 UTC 2020
Assembly of SNAPc, Bdp1, and TBP on the U6 snRNA Gene Promoter in Drosophila melanogaster.
Molecular and cellular biology
Kim MK,Tranvo A,Hurlburt AM,Verma N,Phan P,Luo J,Ranish J,Stumph WE
R960-25 was used in Western Blotting to show that DmSNAPc-Bdp1 can recruit TBP to the U6 promoter, and we identify a region of Bdp1 that is sufficient for TBP recruitment.
Thu May 28 00:00:00 UTC 2020
Tumor Necrosis Factor Alpha Regulates Skeletal Myogenesis by Inhibiting SP1 Interaction with cis-Acting Regulatory Elements within the Fbxl2 Gene Promoter.
Molecular and cellular biology
O'Brien ME,Londino J,McGinnis M,Weathington N,Adair J,Suber T,Kagan V,Chen K,Zou C,Chen B,Bon J,Mallampalli RK
R960-25 was used in Chromatin immunoprecipitation to show that tumor necrosis factor alpha (TNF-α), a critical cytokine linked to the inflammatory response during skeletal muscle regeneration, suppressed Fbxl2 mRNA expression in C2C12 myoblasts and triggered significant alterations in cell cycle, metabolic, and protein translation processes.
Thu May 28 00:00:00 UTC 2020
Regulation of the autophagic PI3KC3 complex by laforin/malin E3-ubiquitin ligase, two proteins involved in Lafora disease.
Biochimica et biophysica acta. Molecular cell research
Sanchez-Martin P,Lahuerta M,Viana R,Knecht E,Sanz P
R960-25 was used in Western Blot, Immunocytochemistry to suggest a new role of the laforin/malin complex in the activation of autophagy via regulation of the PI3KC3 complex and explain the defect in autophagy described in Lafora disease.
Sat Feb 01 00:00:00 UTC 2020
Regulation of the autophagic PI3KC3 complex by laforin/malin E3-ubiquitin ligase, two proteins involved in Lafora disease.
Biochimica et biophysica acta. Molecular cell research
Sanchez-Martin P,Lahuerta M,Viana R,Knecht E,Sanz P
R960-25 was used in Western Blot, Immunocytochemistry to suggest a new role of the laforin/malin complex in the activation of autophagy via regulation of the PI3KC3 complex and explain the defect in autophagy described in Lafora disease.
Sat Feb 01 00:00:00 UTC 2020
Application of Recombination -Induced Tag Exchange (RITE) to study histone dynamics in human cells.
Epigenetics
Molenaar TM,Pagès-Gallego M,Meyn V,van Leeuwen F
R960-25 was used in Western Blotting to study the dynamics of the replication-independent histone variant H3.3 in human cells.
Tue Sep 01 00:00:00 UTC 2020
Trps1 transcription factor represses phosphate-induced expression of SerpinB2 in osteogenic cells.
Bone
Socorro M,Shinde A,Yamazaki H,Khalid S,Monier D,Beniash E,Napierala D
R960-25 was used in Western Blot to establish, if SerpinB2 is involved in function of cells producing mineralized ECM and to determine the interplay between Pi signaling and Trps1 in the regulation of SerpinB2 expression specifically in cells producing mineralized ECM.
Tue Dec 01 00:00:00 UTC 2020
The ESCRT-III complex is required for nuclear pore complex sequestration and regulates gamete replicative lifespan in budding yeast meiosis.
Nucleus (Austin, Tex.)
Koch BA,Staley E,Jin H,Yu HG
R960-25 was used in Western Blotting to demonstrate the importance of ESCRT-III in nuclear envelope remodeling and its potential role in eliminating senescence factors during gametogenesis.
Tue Dec 01 00:00:00 UTC 2020
Decoupling of Apoptosis from Activation of the ER Stress Response by the Drosophila Metallopeptidase superdeath.
Genetics
Palu RAS,Dalton HM,Chow CY
R960-25 was used in Immunohistochemistry to find that the uncharacterized, membrane-bound metallopeptidase CG14516 in Drosophila melanogaster, plays a role in modifying ER stress-induced apoptosis.
Wed Apr 01 00:00:00 UTC 2020
Genome-wide synthetic lethal CRISPR screen identifies FIS1 as a genetic interactor of ALS-linked C9ORF72.
Brain research
Chai N,Haney MS,Couthouis J,Morgens DW,Benjamin A,Wu K,Ousey J,Fang S,Finer S,Bassik MC,Gitler AD
R960-25 was used in Immunoprecipitation to perform a genome-wide synthetic lethal CRISPR screen in human myeloid cells lacking C9ORF72 to have further insight into cellular function of C9ORF72 gene.
Sat Feb 01 00:00:00 UTC 2020
Mutations in the J domain of DNAJB6 cause dominant distal myopathy.
Neuromuscular disorders : NMD
Palmio J,Jonson PH,Inoue M,Sarparanta J,Bengoechea R,Savarese M,Vihola A,Jokela M,Nakagawa M,Noguchi S,Olivé M,Masingue M,Kerty E,Hackman P,Weihl CC,Nishino I,Udd B
R960-25 was used in Immuno-assay to identify two novel mutations in DNAJB6 J domain, c.149C>T (p.A50V) and c.161A>C (p.E54A) as the cause of distal myopathy.
Wed Jan 01 00:00:00 UTC 2020
Nucleoporin 153 links nuclear pore complex to chromatin architecture by mediating CTCF and cohesin binding.
Nature communications
Kadota S,Ou J,Shi Y,Lee JT,Sun J,Yildirim E
R960-25 was used in Immunocytochemistry-immunoflourescence to test the relationship between NUP153–chromatin interactions and gene regulation in pluripotent mouse ES cells.
Mon May 25 00:00:00 UTC 2020
Discovering the anti-cancer potential of non-oncology drugs by systematic viability profiling.
Nature cancer
Corsello SM,Nagari RT,Spangler RD,Rossen J,Kocak M,Bryan JG,Humeidi R,Peck D,Wu X,Tang AA,Wang VM,Bender SA,Lemire E,Narayan R,Montgomery P,Ben-David U,Garvie CW,Chen Y,Rees MG,Lyons NJ,McFarland JM,Wong BT,Wang L,Dumont N,O'Hearn PJ,Stefan E,Doench JG,Harrington CN,Greulich H,Meyerson M,Vazquez F,Subramanian A,Roth JA,Bittker JA,Boehm JS,Mader CC,Tsherniak A,Golub TR
R960-25 was used in Western Blotting to create a public resource containing the growth inhibitory activity of 4,518 drugs tested across 578 human cancer cell lines and screen drugs against cell lines in pools.
Sat Feb 01 00:00:00 UTC 2020
GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system.
BMC biotechnology
Neuhold J,Radakovics K,Lehner A,Weissmann F,Garcia MQ,Romero MC,Berrow NS,Stolt-Bergner P
R960-25 was used in Western Blotting to demonstrate successful construction and expression of three different co-expression systems, the proteosomal lid complex, the anaphase promoting comple.
Tue May 12 00:00:00 UTC 2020
Human GBP1 binds LPS to initiate assembly of a caspase-4 activating platform on cytosolic bacteria.
Nature communications
Santos JC,Boucher D,Schneider LK,Demarco B,Dilucca M,Shkarina K,Heilig R,Chen KW,Lim RYH,Broz P
R960-25 was used in Western Blotting to demonstrate that human GBP1 acts as a cytosolic LPS sensor, assembling a platform that promotes caspase-4 recruitment and activation, the first step in non-canonical inflammasome signaling.
Wed Jun 24 00:00:00 UTC 2020
PRD-2 directly regulates casein kinase I and counteracts nonsense-mediated decay in the Neurospora circadian clock.
eLife
Kelliher CM,Lambreghts R,Xiang Q,Baker CL,Loros JJ,Dunlap JC
R960-25 was used in Western Blotting to find that circadian period defects in two classically-derived Neurospora clock mutants each arise from disruption of ck-1a regulation is consistent with circadian period being exquisitely sensitive to levels of casein kinase I.
Wed Dec 09 00:00:00 UTC 2020
Functional analysis of natural PCSK9 mutants in modern and archaic humans.
The FEBS journal
Mikaeeli S,Susan-Resiga D,Girard E,Ben Djoudi Ouadda A,Day R,Prost S,Seidah NG
R960-25 was used in Western Blotting to reveal that all studied mutations result in PCSK9 LOF, via various mechanisms, leading to lower levels of LDLc.
Sat Feb 01 00:00:00 UTC 2020
Development and characterization of protein kinase B/AKT isoform-specific nanobodies.
PloS one
Merckaert T,Zwaenepoel O,Gevaert K,Gettemans J
R960-25 was used in Western Blotting to develope and characterize nanobodies that specifically interact with the AKT1 or AKT2 isoforms.
Tue Dec 08 00:00:00 UTC 2020
HIV-1 Nef dimers short-circuit immune receptor signaling by activating Tec-family kinases at the host cell membrane.
The Journal of biological chemistry
Li WF,Aryal M,Shu ST,Smithgall TE
R960-25 was used in Immunocytochemistry-Immunofluorescence to conclude that direct activation of Itk and Btk by Nef at the membrane in HIV-infected cells may override normal immune receptor control of Tec-family kinase activity to enhance the viral life cycle.
Fri Apr 10 00:00:00 UTC 2020
An Optimized Chromatin Immunoprecipitation Protocol for Quantification of Protein-DNA Interactions.
STAR protocols
de Jonge WJ,Brok M,Kemmeren P,Holstege FCP
R960-25 was used in Chromatin immunoprecipitation to investigate protein-DNA interactions for the yeast Saccharomyces cerevisiae through a a revised ChIP protocol.
Fri Jun 19 00:00:00 UTC 2020
Activity of Smurf2 Ubiquitin Ligase Is Regulated by the Wnt Pathway Protein Dishevelled.
Cells
Bernatik O,Paclikova P,Sri Ganji R,Bryja V
R960-25 was used in Western Blotting to show that HEK293 cells the deficiency of DVL phenocopies absence of Smurf2 and leads to the increased phosphorylation of R-Smads.
Thu May 07 00:00:00 UTC 2020
Exercise rejuvenates quiescent skeletal muscle stem cells in old mice through restoration of Cyclin D1.
Nature metabolism
Brett JO,Arjona M,Ikeda M,Quarta M,de Morrée A,Egner IM,Perandini LA,Ishak HD,Goshayeshi A,Benjamin DI,Both P,Rodríguez-Mateo C,Betley MJ,Wyss-Coray T,Rando TA
R960-25 was used in Immunocytochemistry-Immunofluorescence to investigate the effect of exercise on aged stem cell function in the context of tissue regeneration in old mice.
Wed Apr 01 00:00:00 UTC 2020
The small EF-hand protein CALML4 functions as a critical myosin light chain within the intermicrovillar adhesion complex.
The Journal of biological chemistry
Choi MS,Graves MJ,Matoo S,Storad ZA,El Sheikh Idris RA,Weck ML,Smith ZB,Tyska MJ,Crawley SW
R960-25 was used in Western Blotting to the molecular composition of the intermicrovillar adhesion complex (IMAC) using a protein isolation strategy to recover components of the complex associated with CDHR2 (protocadherin-24).
Fri Jul 10 00:00:00 UTC 2020
NAA10 p.(D10G) and NAA10 p.(L11R) Variants Hamper Formation of the NatA N-Terminal Acetyltransferase Complex.
International journal of molecular sciences
McTiernan N,Darbakk C,Ree R,Arnesen T
R960-25 was used in Western Blotting to analyse the functions of two de novo NAA10 catalytic subunit variants of N-terminal acetyltransferases (NATs) complex, c.29A>G p.(D10G) and c.32T>G p.(L11R),.
Thu Nov 26 00:00:00 UTC 2020
A-kinase-anchoring protein 1 (dAKAP1)-based signaling complexes coordinate local protein synthesis at the mitochondrial surface.
The Journal of biological chemistry
Gabrovsek L,Collins KB,Aggarwal S,Saunders LM,Lau HT,Suh D,Sancak Y,Trapnell C,Ong SE,Smith FD,Scott JD
R960CUS was used in Immunoprecipitation to demonstrate the role of dual-specificity protein kinase A anchoring protein as a coordinator of second messenger signaling and local protein synthesis machinery.
Fri Jul 31 00:00:00 UTC 2020
Severe syndromic ID and skewed X-inactivation in a girl with NAA10 dysfunction and a novel heterozygous de novo NAA10 p.(His16Pro) variant - a case report.
BMC medical genetics
Bader I,McTiernan N,Darbakk C,Boltshauser E,Ree R,Ebner S,Mayr JA,Arnesen T
R960-25 was used in Immunoprecipitation to demonstrate that, in a 10-year-old girl with a novel de novo NAA10 variant, NAA10 p.(His16Pro) causes a severe form of syndromic intellectual disability (ID), through impaired NatA-mediated Nt-acetylation of cellular proteins, and preferential methylation/inactivation of maternally inherited alleles.
Wed Jul 22 00:00:00 UTC 2020
Spt6 is a maintenance factor for centromeric CENP-A.
Nature communications
Bobkov GOM,Huang A,van den Berg SJW,Mitra S,Anselm E,Lazou V,Schunter S,Feederle R,Imhof A,Lusser A,Jansen LET,Heun P
R960-25 was used in Immunocytochemistry to demonstrate that the histone chaperone and transcription elongation factor Spt6 spatially and temporarily coincides with centromeric transcription and prevents the loss of old CENP-A nucleosomes in both Drosophila and human cells.
Wed Jun 10 00:00:00 UTC 2020
Twin enzymes, divergent control: The cholesterogenic enzymes DHCR14 and LBR are differentially regulated transcriptionally and post-translationally.
The Journal of biological chemistry
Capell-Hattam IM,Sharpe LJ,Qian L,Hart-Smith G,Prabhu AV,Brown AJ
R960-25 was used in Western Blot, Immunoprecipitation to indicate that whereas LBR tends to be the constitutively active C14-sterol reductase, DHCR14 levels are tunable, responding to the local cellular demands for cholesterol.
Fri Feb 28 00:00:00 UTC 2020
Twin enzymes, divergent control: The cholesterogenic enzymes DHCR14 and LBR are differentially regulated transcriptionally and post-translationally.
The Journal of biological chemistry
Capell-Hattam IM,Sharpe LJ,Qian L,Hart-Smith G,Prabhu AV,Brown AJ
R960-25 was used in Western Blot, Immunoprecipitation to indicate that whereas LBR tends to be the constitutively active C14-sterol reductase, DHCR14 levels are tunable, responding to the local cellular demands for cholesterol.
Fri Feb 28 00:00:00 UTC 2020
Sequential activation of transcriptional repressors promotes progenitor commitment by silencing stem cell identity genes.
eLife
Rives-Quinto N,Komori H,Ostgaard CM,Janssens DH,Kondo S,Dai Q,Moore AW,Lee CY
R960-25 was used in Immunocytochemistry to propose that continual inactivation of stem cell identity genes allows intermediate progenitors to stably commit to generating diverse differentiated cells during indirect neurogenesis.
Thu Nov 26 00:00:00 UTC 2020
Systematic Screen for Drosophila Transcriptional Regulators Phosphorylated in Response to Insulin/mTOR Pathway.
G3 (Bethesda, Md.)
Liu Y,Mattila J,Hietakangas V
R960-25 was used in Western Blotting to report the results of an unbiased screen for Drosophila TRs phosphorylated in an IIS-dependent manner.
Wed Aug 05 00:00:00 UTC 2020
Long isoforms of NRF1 negatively regulate adipogenesis via suppression of PPARγ expression.
Redox biology
Xue P,Hou Y,Zuo Z,Wang Z,Ren S,Dong J,Fu J,Wang H,Andersen ME,Zhang Q,Xu Y,Pi J
R960-25 was used in Western Blot to provide further evidence for a novel role of NRF1 beyond its participation in cellular antioxidant response and suggest that L-NRF1 is a negative regulator of PPARγ2 expression and thereby can suppress adipogenesis.
Sat Feb 01 00:00:00 UTC 2020
Long isoforms of NRF1 negatively regulate adipogenesis via suppression of PPARγ expression.
Redox biology
Xue P,Hou Y,Zuo Z,Wang Z,Ren S,Dong J,Fu J,Wang H,Andersen ME,Zhang Q,Xu Y,Pi J
R960-25 was used in Western Blot to provide further evidence for a novel role of NRF1 beyond its participation in cellular antioxidant response and suggest that L-NRF1 is a negative regulator of PPARγ2 expression and thereby can suppress adipogenesis.
Sat Feb 01 00:00:00 UTC 2020
A single-parasite transcriptional atlas of Toxoplasma Gondii reveals novel control of antigen expression.
eLife
Xue Y,Theisen TC,Rastogi S,Ferrel A,Quake SR,Boothroyd JC
R960-25 was used in Immunocytochemistry-immunoflourescence to analyze the composition and dynamics of the signalosomes assembling around each of the tagged proteins.
Mon Feb 17 00:00:00 UTC 2020
Phosphorylation of seryl-tRNA synthetase by ATM/ATR is essential for hypoxia-induced angiogenesis.
PLoS biology
Shi Y,Liu Z,Zhang Q,Vallee I,Mo Z,Kishi S,Yang XL
R960CUS was used in Western Blotting to reveal a key step regulating hypoxic angiogenesis and highlight the importance of nuclear SerRS in post-developmental angiogenesis regulation in addition to vascular development.
Tue Dec 01 00:00:00 UTC 2020
Genome-wide off-rates reveal how DNA binding dynamics shape transcription factor function.
Molecular systems biology
de Jonge WJ,Brok M,Lijnzaad P,Kemmeren P,Holstege FC
R960-25 was used in Immunoprecipitation to show how off-rates contribute to transcription factor function and that DIVORSEQ (Determining In Vivo Off-Rates by SEQuencing) is a meaningful way of investigating protein-DNA binding dynamics genome-wide.
Thu Oct 01 00:00:00 UTC 2020
A novel homozygous variant in MICOS13/QIL1 causes hepato-encephalopathy with mitochondrial DNA depletion syndrome.
Molecular genetics & genomic medicine
Kishita Y,Shimura M,Kohda M,Akita M,Imai-Okazaki A,Yatsuka Y,Nakajima Y,Ito T,Ohtake A,Murayama K,Okazaki Y
R960-25 was used in Western Blotting to determine the clinical significance of the identified variant.
Thu Oct 01 00:00:00 UTC 2020
A Thalamic Orphan Receptor Drives Variability in Short-Term Memory.
Cell
Hsiao K,Noble C,Pitman W,Yadav N,Kumar S,Keele GR,Terceros A,Kanke M,Conniff T,Cheleuitte-Nieves C,Tolwani R,Sethupathy P,Rajasethupathy P
R960-25 was used in Western Blotting to identify an orphan receptor as a potent modifier of short-term memory and supplement classical PFC-based models with an emerging thalamus-centric framework for the mechanistic understanding of working memory.
Thu Oct 15 00:00:00 UTC 2020
Miga-mediated endoplasmic reticulum-mitochondria contact sites regulate neuronal homeostasis.
eLife
Xu L,Wang X,Zhou J,Qiu Y,Shang W,Liu JP,Wang L,Tong C
R960-25 was used in Western Blot, Immunohistochemistry, Immunocytochemistry to investigate the molecular organization, functions, regulation of ERMCS, and the physiological roles of altered ERMCSs in higher eukaryotes.
Fri Jul 10 00:00:00 UTC 2020
Miga-mediated endoplasmic reticulum-mitochondria contact sites regulate neuronal homeostasis.
eLife
Xu L,Wang X,Zhou J,Qiu Y,Shang W,Liu JP,Wang L,Tong C
R960-25 was used in Western Blot, Immunohistochemistry, Immunocytochemistry to investigate the molecular organization, functions, regulation of ERMCS, and the physiological roles of altered ERMCSs in higher eukaryotes.
Fri Jul 10 00:00:00 UTC 2020
Miga-mediated endoplasmic reticulum-mitochondria contact sites regulate neuronal homeostasis.
eLife
Xu L,Wang X,Zhou J,Qiu Y,Shang W,Liu JP,Wang L,Tong C
R960-25 was used in Western Blot, Immunohistochemistry, Immunocytochemistry to investigate the molecular organization, functions, regulation of ERMCS, and the physiological roles of altered ERMCSs in higher eukaryotes.
Fri Jul 10 00:00:00 UTC 2020
BAG3 Pro209 mutants associated with myopathy and neuropathy relocate chaperones of the CASA-complex to aggresomes.
Scientific reports
Adriaenssens E,Tedesco B,Mediani L,Asselbergh B,Crippa V,Antoniani F,Carra S,Poletti A,Timmerman V
R960-25 was used in Immunoprecipitation to study the molecular mechanisms of three heterozygous Pro209 mutations in BAG3 in patients.
Fri May 29 00:00:00 UTC 2020
The tumor suppressor PTPRK promotes ZNRF3 internalization and is required for Wnt inhibition in the Spemann organizer.
eLife
Chang LS,Kim M,Glinka A,Reinhard C,Niehrs C
R960-25 was used in Immunocytochemistry to identify the tumor suppressor Protein tyrosine phosphatase receptor-type kappa, as a Wnt inhibitor in human cancer cells and in the Spemann organizer of Xenopus embryos.
Tue Jan 14 00:00:00 UTC 2020
The tumor suppressor PTPRK promotes ZNRF3 internalization and is required for Wnt inhibition in the Spemann organizer.
eLife
Chang LS,Kim M,Glinka A,Reinhard C,Niehrs C
R960-25 was used in Immunocytochemistry to identify the tumor suppressor Protein tyrosine phosphatase receptor-type kappa, as a Wnt inhibitor in human cancer cells and in the Spemann organizer of Xenopus embryos.
Tue Jan 14 00:00:00 UTC 2020
Distinct roles of structure-specific endonucleases EEPD1 and Metnase in replication stress responses.
NAR cancer
Sharma N,Speed MC,Allen CP,Maranon DG,Williamson E,Singh S,Hromas R,Nickoloff JA
R960-25 was used in Western Blotting to demonstrate that Metnase is also recruited to stalled forks where it appears to dimethylate histone H3 lysine 36 (H3K36me2), raising the possibility that H3K36me2 promotes DDR factor recruitment or limits nucleosome eviction to protect forks from nucleolytic attack.
Mon Jun 01 00:00:00 UTC 2020
Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.
Scientific reports
Soliman SHA,Stark AE,Gardner ML,Harshman SW,Breece CC,Amari F,Orlacchio A,Chen M,Tessari A,Martin JA,Visone R,Freitas MA,La Perle KMD,Palmieri D,Coppola V
R960-25 was used in Immunoprecipitation to report the generation of a new mouse line in which RanBP9 expression and interactions can be reliably studied by the use of commercially available αtag antibodies.
Tue Apr 28 00:00:00 UTC 2020
Functional Landscape of SARS-CoV-2 Cellular Restriction.
bioRxiv : the preprint server for biology
Martin-Sancho L,Lewinski MK,Pache L,Stoneham CA,Yin X,Pratt D,Churas C,Rosenthal SB,Liu S,De Jesus PD,O'Neill AM,Gounder AP,Nguyen C,Pu Y,Oom AL,Miorin L,Rodriguez-Frandsen A,Urbanowski M,Shaw ML,Chang MW,Benner C,Frieman MB,García-Sastre A,Ideker T,Hultquist JF,Guatelli J,Chanda SK
R960-25 was used in Western Blotting to analyse and evaluate the impact of human interferon stimulated genes on viral replication.
Wed Sep 30 00:00:00 UTC 2020
Dengue Virus Infection of Aedes aegypti Alters Extracellular Vesicle Protein Cargo to Enhance Virus Transmission.
International journal of molecular sciences
Gold AS,Feitosa-Suntheimer F,Araujo RV,Hekman RM,Asad S,Londono-Renteria B,Emili A,Colpitts TM
R960-25 was used in Western Blotting to investigate the function of Aedes aegypti mosquito extracellular vesicle cargo during dengue infection.
Thu Sep 10 00:00:00 UTC 2020
The fibronectin type-III (FNIII) domain of ATF7IP contributes to efficient transcriptional silencing mediated by the SETDB1 complex.
Epigenetics & chromatin
Tsusaka T,Fukuda K,Shimura C,Kato M,Shinkai Y
R960-25 was used in Immunoprecipitation to contribute to further understanding the function of ATF7IP in the SETDB1 complex.
Mon Nov 30 00:00:00 UTC 2020
Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis.
Nature communications
van der Wel T,Hilhorst R,den Dulk H,van den Hooven T,Prins NM,Wijnakker JAPM,Florea BI,Lenselink EB,van Westen GJP,Ruijtenbeek R,Overkleeft HS,Kaptein A,Barf T,van der Stelt M
R960-25 was used in Western Blotting to describe a chemical genetics strategy to selectively study target engagement of endogenous kinases.
Thu Jun 25 00:00:00 UTC 2020
Sorting nexin 17 (SNX17) links endosomal sorting to Eps15 homology domain protein 1 (EHD1)-mediated fission machinery.
The Journal of biological chemistry
Dhawan K,Naslavsky N,Caplan S
R960-25 was used in Immunocytochemistry to study the regulatory mechanism of SNX17-interacting receptor recycling by SNX17 and EHD1.
Fri Mar 20 00:00:00 UTC 2020
The role of Rif1 in telomere length regulation is separable from its role in origin firing.
eLife
Shubin CB,Greider CW
R960-25 was used in Western Blotting to conclude that the Rif1 function in telomere length regulation is separate from its function in PP1 recruitment and regulation of origin firing.
Mon Jun 29 00:00:00 UTC 2020
Paraoxonase 3 functions as a chaperone to decrease functional expression of the epithelial sodium channel.
The Journal of biological chemistry
Shi S,Montalbetti N,Wang X,Rush BM,Marciszyn AL,Baty CJ,Tan RJ,Carattino MD,Kleyman TR
R960-25 was used in Immunoprecipitation to investigate the modulatory role of the PON family in in ion channel expression.
Fri Apr 10 00:00:00 UTC 2020
A homozygous missense variant in CACNB4 encoding the auxiliary calcium channel beta4 subunit causes a severe neurodevelopmental disorder and impairs channel and non-channel functions.
PLoS genetics
Coste de Bagneaux P,von Elsner L,Bierhals T,Campiglio M,Johannsen J,Obermair GJ,Hempel M,Flucher BE,Kutsche K
R960-25 was used in Immunocytochemistry to investigate the functional implications of a CACNB4 missense variant in siblings with severe neurological disorder.
Sun Mar 01 00:00:00 UTC 2020
RNA-recognition motif in Matrin-3 mediates neurodegeneration through interaction with hnRNPM.
Acta neuropathologica communications
Ramesh N,Kour S,Anderson EN,Rajasundaram D,Pandey UB
R960-25 was used in Western Blot, RNA Immunoprecipitation to investigate the molecular mechanisms underlying MATR3-mediated neuromuscular degeneration.
Tue Aug 18 00:00:00 UTC 2020
RNA-recognition motif in Matrin-3 mediates neurodegeneration through interaction with hnRNPM.
Acta neuropathologica communications
Ramesh N,Kour S,Anderson EN,Rajasundaram D,Pandey UB
R960-25 was used in Western Blot, RNA Immunoprecipitation to investigate the molecular mechanisms underlying MATR3-mediated neuromuscular degeneration.
Tue Aug 18 00:00:00 UTC 2020
The MARCH6-SQLE Axis Controls Endothelial Cholesterol Homeostasis and Angiogenic Sprouting.
Cell reports
Tan JME,van der Stoel MM,van den Berg M,van Loon NM,Moeton M,Scholl E,van der Wel NN,Kovačević I,Hordijk PL,Loregger A,Huveneers S,Zelcer N
R960-25 was used in Western Blot to propose a mechanistic link between regulation of cholesterol homeostasis by the MARCH6-SQLE axis and endothelial integrity and angiogenesis.
Tue Aug 04 00:00:00 UTC 2020
ISG15-dependent Activation of the RNA Sensor MDA5 and its Antagonism by the SARS-CoV-2 papain-like protease.
bioRxiv : the preprint server for biology
Liu G,Lee JH,Parker ZM,Acharya D,Chiang JJ,van Gent M,Riedl W,Davis-Gardner ME,Wies E,Chiang C,Gack MU
R960-25 was used in Western Blotting to demonstrate interferon-stimulated gene ISG15 is crucial for the ability of viral RNA sensor MDA5 to perform an antiviral response.
Tue Oct 27 00:00:00 UTC 2020
Genetic modifiers ameliorate endocytic and neuromuscular defects in a model of spinal muscular atrophy.
BMC biology
Walsh MB,Janzen E,Wingrove E,Hosseinibarkooie S,Muela NR,Davidow L,Dimitriadi M,Norabuena EM,Rubin LL,Wirth B,Hart AC
R960-25 was used in Western Blotting to propose that hnRNP F/H act in the same protein complex as PLS3 and SMN and that the function of this complex is critical for endocytic pathways, suggesting that hnRNP F/H proteins could be potential targets for therapy development.
Wed Sep 16 00:00:00 UTC 2020
Loss of N-Glycanase 1 Alters Transcriptional and Translational Regulation in K562 Cell Lines.
G3 (Bethesda, Md.)
Mueller WF,Jakob P,Sun H,Clauder-Münster S,Ghidelli-Disse S,Ordonez D,Boesche M,Bantscheff M,Collier P,Haase B,Benes V,Paulsen M,Sehr P,Lewis J,Drewes G,Steinmetz LM
R960-25 was used in Western Blotting to emonstrate that the compound NVP-BEZ235 (Dactosilib) promotes degradation of N-Glycanase 1-dependent substrates, concurrent with increased autophagic flux.
Mon May 04 00:00:00 UTC 2020
High Throughput Screening of FDA-Approved Drug Library Reveals the Compounds that Promote IRF3-Mediated Pro-Apoptotic Pathway Inhibit Virus Replication.
Viruses
Glanz A,Chawla K,Fabry S,Subramanian G,Garcia J,Jay B,Ciricillo J,Chakravarti R,Taylor RT,Chattopadhyay S
R960-25 was used in Western Blotting to explore whether the RIPA branch of IRF3 can be targeted therapeutically to prevent virus infection.
Tue Apr 14 00:00:00 UTC 2020
The coordinate actions of calcineurin and Hog1 mediate the stress response through multiple nodes of the cell cycle network.
PLoS genetics
Leech CM,Flynn MJ,Arsenault HE,Ou J,Liu H,Zhu LJ,Benanti JA
R960-25 was used in Western Blotting to investigate the role of the phosphatase calcineurin (CN) in the stress response and demonstrate that CN activates the Hog1/p38 pathway in both yeast and human cells.
Wed Apr 01 00:00:00 UTC 2020
Telomerase Repairs Collapsed Replication Forks at Telomeres.
Cell reports
Matmati S,Lambert S,Géli V,Coulon S
R960-25 was used in Chromatin immunoprecipitation to investigate, in fission yeast, telomere replication dynamics in telomerase-negative cells to unmask problems associated with telomere replication.
Tue Mar 10 00:00:00 UTC 2020
DDX5 resolves R-loops at DNA double-strand breaks to promote DNA repair and avoid chromosomal deletions.
NAR cancer
Yu Z,Mersaoui SY,Guitton-Sert L,Coulombe Y,Song J,Masson JY,Richard S
R960-25 was used in Immunocytochemistry to define a role for DDX5 in facilitating the clearance of RNA transcripts overlapping DSBs to ensure proper DNA repair.
Tue Sep 01 00:00:00 UTC 2020
S-acylation modulates the function of the apical sodium-dependent bile acid transporter in human cells.
The Journal of biological chemistry
Ticho AL,Malhotra P,Manzella CR,Dudeja PK,Saksena S,Gill RK,Alrefai WA
R960-25 was used in Western Blotting to provide evidence that S-acylation is involved in the modulation of apical sodium-dependent bile acid transporter function.
Fri Apr 03 00:00:00 UTC 2020
Both EZH2 and JMJD6 regulate cell cycle genes in breast cancer.
BMC cancer
Biswas A,Mukherjee G,Kondaiah P,Desai KV
R960-25 was used in Western Blotting to understand additional pathways activated by EZH2 and JMJD6 in regulating cell cycle genes in breast cancer.
Fri Nov 27 00:00:00 UTC 2020
Myristoylated alanine-rich C kinase substrate-like protein-1 regulates epithelial sodium channel activity in renal distal convoluted tubule cells.
American journal of physiology. Cell physiology
Song C,Yue Q,Moseley A,Al-Khalili O,Wynne BM,Ma H,Wang L,Eaton DC
R960-25 was used in Western Blot to suggest a complicated role for proteolytic processing in MLP-1 regulation of ENaC.
Tue Sep 01 00:00:00 UTC 2020
Tyrosine nitration on calmodulin enhances calcium-dependent association and activation of nitric-oxide synthase.
The Journal of biological chemistry
Porter JJ,Jang HS,Haque MM,Stuehr DJ,Mehl RA
R960-25 was used in Immunoprecipitation to indicate that the nitroTyr post-translational modification, like tyrosine phosphorylation, can impact calmodulin sensitivity for calcium and reveal Tyr site-specific gain or loss of functions for calmodulin-induced eNOS activation.
Fri Feb 21 00:00:00 UTC 2020
SALL4 promotes tumor progression in breast cancer by targeting EMT.
Molecular carcinogenesis
Chen T,Tsang JYS,Su XC,Li P,Sun WQ,Wong ILK,Choy KY,Yang Q,Tse GMK,Chan TH,Chow LMC
R960-25 was used in Western Blotting to hypothesize that SALL4 promotes tumor progression in breast cancer by inducing the mesenchymal markers like vimentin through directly binding to its promoter.
Thu Oct 01 00:00:00 UTC 2020
Fibroblast growth factor homologous factors tune arrhythmogenic late NaV1.5 current in calmodulin binding-deficient channels.
JCI insight
Abrams J,Roybal D,Chakouri N,Katchman AN,Weinberg R,Yang L,Chen BX,Zakharov SI,Hennessey JA,Avula UMR,Diaz J,Wang C,Wan EY,Pitt GS,Ben-Johny M,Marx SO
R960-25 was used in Immunocytochemistry to determine regulatory factors that prevent late Na+ current for the IQ/AA mutant channel.
Fri Oct 02 00:00:00 UTC 2020
Specialization of the chromatin remodeler RSC to mobilize partially-unwrapped nucleosomes.
eLife
Schlichter A,Kasten MM,Parnell TJ,Cairns BR
R960-25 was used in Immunoprecipitation to show Rsc1/2 isoforms are specialized through composition and interactions to manage and remodel partially-unwrapped nucleosomes.
Thu Jun 04 00:00:00 UTC 2020
HIV-1 Vpr induces cell cycle arrest and enhances viral gene expression by depleting CCDC137.
eLife
Zhang F,Bieniasz PD
R960-25 was used in Western Blot, Immunocytochemistry to identify the CCDC137/cPERP-B as a target protein of HIV-1 Vpr accessory protein-induced ubiquitin/proteasome-dependent degradatio which promotes cell-cycle arrest and HIV-1 gene expression.
Mon Jun 15 00:00:00 UTC 2020
HIV-1 Vpr induces cell cycle arrest and enhances viral gene expression by depleting CCDC137.
eLife
Zhang F,Bieniasz PD
R960-25 was used in Western Blot, Immunocytochemistry to identify the CCDC137/cPERP-B as a target protein of HIV-1 Vpr accessory protein-induced ubiquitin/proteasome-dependent degradatio which promotes cell-cycle arrest and HIV-1 gene expression.
Mon Jun 15 00:00:00 UTC 2020
Mutations in FAM50A suggest that Armfield XLID syndrome is a spliceosomopathy.
Nature communications
Lee YR,Khan K,Armfield-Uhas K,Srikanth S,Thompson NA,Pardo M,Yu L,Norris JW,Peng Y,Gripp KW,Aleck KA,Li C,Spence E,Choi TI,Kwon SJ,Park HM,Yu D,Heo WD,Mooney MR,Baig SM,Wentzensen IM,Telegrafi A,McWalter K,Moreland T,Roadhouse C,Ramsey K,Lyons MJ,Skinner C,Alexov E,Katsanis N,Stevenson RE,Choudhary JS,Adams DJ,Kim CH,Davis EE,Schwartz CE
R960-25 was used in Immunocytochemistry-immunoflourescence to report rare FAM50A missense variants in the original Armfield XLID syndrome family localized in Xq28 and four additional unrelated males with overlapping features.
Thu Jul 23 00:00:00 UTC 2020
CRISPR-Csy4-Mediated Editing of Rotavirus Double-Stranded RNA Genome.
Cell reports
Papa G,Venditti L,Braga L,Schneider E,Giacca M,Petris G,Burrone OR
R960-25 was used in Western Blot, Immunocytochemistry to achieve the nuclease-mediated genome editing of rotavirus, an important human and livestock pathogen with a multisegmented dsRNA genome.
Tue Sep 29 00:00:00 UTC 2020
CRISPR-Csy4-Mediated Editing of Rotavirus Double-Stranded RNA Genome.
Cell reports
Papa G,Venditti L,Braga L,Schneider E,Giacca M,Petris G,Burrone OR
R960-25 was used in Western Blot, Immunocytochemistry to achieve the nuclease-mediated genome editing of rotavirus, an important human and livestock pathogen with a multisegmented dsRNA genome.
Tue Sep 29 00:00:00 UTC 2020
Ubiquitin-interacting motifs of ataxin-3 regulate its polyglutamine toxicity through Hsc70-4-dependent aggregation.
eLife
Johnson SL,Ranxhi B,Libohova K,Tsou WL,Todi SV
R960-25 was used in Western Blotting to determine the role of ataxin-3's non-polyglutamine domains in disease.
Mon Sep 21 00:00:00 UTC 2020
Proximity labeling in mammalian cells with TurboID and split-TurboID.
Nature protocols
Cho KF,Branon TC,Udeshi ND,Myers SA,Carr SA,Ting AY
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, Immunohistochemistry to describe the use of TurboID and split-TurboID in proximity labeling applications for mapping protein-protein interactions and subcellular proteomes in live mammalian cells.
Tue Dec 01 00:00:00 UTC 2020
A genetic screen in Drosophila reveals an unexpected role for the KIP1 ubiquitination-promoting complex in male fertility.
PLoS genetics
Li W,Liang J,Outeda P,Turner S,Wakimoto BT,Watnick T
R960-25 was used in Western Blot, Immunoprecipitation to identify a new complex that is involved in male fertility in Drosophila melanogaster.
Tue Dec 01 00:00:00 UTC 2020
A genetic screen in Drosophila reveals an unexpected role for the KIP1 ubiquitination-promoting complex in male fertility.
PLoS genetics
Li W,Liang J,Outeda P,Turner S,Wakimoto BT,Watnick T
R960-25 was used in Western Blot, Immunoprecipitation to identify a new complex that is involved in male fertility in Drosophila melanogaster.
Tue Dec 01 00:00:00 UTC 2020
Complement-Independent Modulation of Influenza A Virus Infection by Factor H.
Frontiers in immunology
Murugaiah V,Varghese PM,Saleh SM,Tsolaki AG,Alrokayan SH,Khan HA,Collison KS,Sim RB,Nal B,Al-Mohanna FA,Kishore U
R960-25 was used in Immunocytochemistry to report that factor H, a sialic acid binder, interacts with influenza A virus (IAV) and modulates IAV entry, as evident from down-regulation of matrix protein 1 (M1) in H1N1 subtype-infected cells and up-regulation of M1 expression in H3N2-infected A549 cells.
Mon Mar 01 00:00:00 UTC 2021
Dynamic Lamin B1-Gene Association During Oligodendrocyte Progenitor Differentiation.
Neurochemical research
Yattah C,Hernandez M,Huang D,Park H,Liao W,Casaccia P
R960-25 was used in Immunocytochemistry to identify Lss, the gene encoding for lanosterol synthase, a key enzyme in cholesterol synthesis, as associated to the nuclear lamina in OLLMNB1-Dam.
Sun Mar 01 00:00:00 UTC 2020
Dynamic Lamin B1-Gene Association During Oligodendrocyte Progenitor Differentiation.
Neurochemical research
Yattah C,Hernandez M,Huang D,Park H,Liao W,Casaccia P
R960-25 was used in Immunocytochemistry to identify Lss, the gene encoding for lanosterol synthase, a key enzyme in cholesterol synthesis, as associated to the nuclear lamina in OLLMNB1-Dam.
Sun Mar 01 00:00:00 UTC 2020
Systematized reporter assays reveal ZIC protein regulatory abilities are Subclass-specific and dependent upon transcription factor binding site context.
Scientific reports
Ahmed JN,Diamand KEM,Bellchambers HM,Arkell RM
R960-25 was used in Western Blotting to assess the likely pathogenicity of variant ZIC proteins associated with human disorders and for determining factors that influence functional transcription factor binding.
Tue Aug 04 00:00:00 UTC 2020
Chemical inhibition of FBXO7 reduces inflammation and confers neuroprotection by stabilizing the mitochondrial kinase PINK1.
JCI insight
Liu Y,Lear TB,Verma M,Wang KZ,Otero PA,McKelvey AC,Dunn SR,Steer E,Bateman NW,Wu C,Jiang Y,Weathington NM,Rojas M,Chu CT,Chen BB,Mallampalli RK
R960-25 was used in Immunoprecipitation to show that a ubiquitin E3 ligase receptor component, FBXO7, targets (PTEN-induced kinase 1)PINK1 for its cellular disposal and induces mitochondrial injury and inflammation in experimental pneumonia.
Thu Jun 04 00:00:00 UTC 2020
Yeast Surface Display System for Facilitated Production and Application of Phage Endolysin.
ACS synthetic biology
Chun J,Bai J,Ryu S
R960-25 was used in Western Blot, Flow Cytometry to suggest that the yeast surface display system is an efficient, stable, and straightforward platform for the production and antibacterial applications of endolysin.
Fri Mar 20 00:00:00 UTC 2020
Yeast Surface Display System for Facilitated Production and Application of Phage Endolysin.
ACS synthetic biology
Chun J,Bai J,Ryu S
R960-25 was used in Western Blot, Flow Cytometry to suggest that the yeast surface display system is an efficient, stable, and straightforward platform for the production and antibacterial applications of endolysin.
Fri Mar 20 00:00:00 UTC 2020
Phenotypic and genotypic characterization of families with complex intellectual disability identified pathogenic genetic variations in known and novel disease genes.
Scientific reports
Darvish H,Azcona LJ,Tafakhori A,Mesias R,Ahmadifard A,Sanchez E,Habibi A,Alehabib E,Johari AH,Emamalizadeh B,Jamali F,Chapi M,Jamshidi J,Kajiwara Y,Paisán-Ruiz C
R960-25 was used in Western Blot to recruit seven different recessive NDD families with comorbidities to perform a detailed clinical characterization and a complete genomic analysis that consists of a combination of high throughput SNP-based genotyping and whole-genome sequencing (WGS).
Wed Jan 22 00:00:00 UTC 2020
Phenotypic and genotypic characterization of families with complex intellectual disability identified pathogenic genetic variations in known and novel disease genes.
Scientific reports
Darvish H,Azcona LJ,Tafakhori A,Mesias R,Ahmadifard A,Sanchez E,Habibi A,Alehabib E,Johari AH,Emamalizadeh B,Jamali F,Chapi M,Jamshidi J,Kajiwara Y,Paisán-Ruiz C
R960-25 was used in Western Blot to recruit seven different recessive NDD families with comorbidities to perform a detailed clinical characterization and a complete genomic analysis that consists of a combination of high throughput SNP-based genotyping and whole-genome sequencing (WGS).
Wed Jan 22 00:00:00 UTC 2020
Targeted integration by homologous recombination enables in situ tagging and replacement of genes in the marine microeukaryote Diplonema papillatum.
Environmental microbiology
Faktorová D,Kaur B,Valach M,Graf L,Benz C,Burger G,Lukeš J
R960-25 was used in Western Blot to provide tools that allow gene tagging, knock-out and knock-in strategies in D. papillatum and likely other diplonemids.
Tue Sep 01 00:00:00 UTC 2020
The human mitochondrial enzyme BCO2 exhibits catalytic activity toward carotenoids and apocarotenoids.
The Journal of biological chemistry
Thomas LD,Bandara S,Parmar VM,Srinivasagan R,Khadka N,Golczak M,Kiser PD,von Lintig J
R960-25 was used in Western Blotting to demonstrate that human BCO2 is a catalytically competent enzyme. Consequently, information on BCO2 becomes broadly applicable in human biology with important implications for the physiology of the eyes and other tissues.
Fri Nov 13 00:00:00 UTC 2020
Intracellular autoactivation of TMPRSS11A, an airway epithelial transmembrane serine protease.
The Journal of biological chemistry
Zhang C,Zhang Y,Zhang S,Wang Z,Sun S,Liu M,Chen Y,Dong N,Wu Q
R960-25 was used in Flow cytometry/Cell sorting to provide new insights into the diverse mechanisms in regulating TTSP activation.
Fri Sep 04 00:00:00 UTC 2020
Loss of USF2 promotes proliferation, migration and mitophagy in a redox-dependent manner.
Redox biology
Chi TF,Khoder-Agha F,Mennerich D,Kellokumpu S,Miinalainen I,Kietzmann T,Dimova EY
R960-25 was used in Western Blotting to investigate the functions of upstream stimulatory factor 2 (USF2) in tumor proliferation and migration.
Thu Oct 01 00:00:00 UTC 2020
The interferon-inducible protein TDRD7 inhibits AMP-activated protein kinase and thereby restricts autophagy-independent virus replication.
The Journal of biological chemistry
Subramanian G,Popli S,Chakravarty S,Taylor RT,Chakravarti R,Chattopadhyay S
R960-25 was used in Western Blot to identify a new viral restriction factor which inhibited herpes simplex replication.
Fri May 15 00:00:00 UTC 2020
TMEM16K is an interorganelle regulator of endosomal sorting.
Nature communications
Petkovic M,Oses-Prieto J,Burlingame A,Jan LY,Jan YN
R960-25 was used in Immunocytochemistry-immunoflourescence to report that TMEM16K, an endoplasmic reticulum lipid scramblase causative for spinocerebellar ataxia (SCAR10), is an interorganelle regulator of the endolysosomal pathway.
Fri Jul 03 00:00:00 UTC 2020
Molecular basis for N-terminal acetylation by human NatE and its modulation by HYPK.
Nature communications
Deng S,McTiernan N,Wei X,Arnesen T,Marmorstein R
R960-25 was used in Immunoprecipitation to reveal the molecular basis for coordinated N-terminal acetylation by NatE and HYPK.
Mon Feb 10 00:00:00 UTC 2020
Asparagine Hydroxylation is a Reversible Post-translational Modification.
Molecular & cellular proteomics : MCP
Rodriguez J,Haydinger CD,Peet DJ,Nguyen LK,von Kriegsheim A
R960-25 was used in Western Blotting to present evidence that the cellular machinery can reverse FIH-mediated asparagine hydroxylation on intact proteins.
Sun Nov 01 00:00:00 UTC 2020
PrimPol-dependent single-stranded gap formation mediates homologous recombination at bulky DNA adducts.
Nature communications
Piberger AL,Bowry A,Kelly RDW,Walker AK,González-Acosta D,Bailey LJ,Doherty AJ,Méndez J,Morris JR,Bryant HE,Petermann E
R960-25 was used in Western Blot to show that homologous recombination induced by bulky adducts in mammalian cells predominantly occurs at post-replicative gaps formed by the DNA/RNA primase PrimPol.
Tue Nov 17 00:00:00 UTC 2020
S-phase-independent silencing establishment in Saccharomyces cerevisiae.
eLife
Goodnight D,Rine J
R960-25 was used in Western Blotting to reveal that silencing establishment is absolutely dependent on the cell-cycle-regulated interplay between euchromatic and heterochromatic histone modifications.
Mon Jul 20 00:00:00 UTC 2020
Wrapping glia regulates neuronal signaling speed and precision in the peripheral nervous system of Drosophila.
Nature communications
Kottmeier R,Bittern J,Schoofs A,Scheiwe F,Matzat T,Pankratz M,Klämbt C
R960-25 was used in Immunohistochemistry to investigate how glia regulate axonal properties in Dropshilia larvae.
Tue Sep 08 00:00:00 UTC 2020
Sperm-specific COX6B2 enhances oxidative phosphorylation, proliferation, and survival in human lung adenocarcinoma.
eLife
Cheng CC,Wooten J,Gibbs ZA,McGlynn K,Mishra P,Whitehurst AW
R960-25 was used in Western Blotting to reveal the tumor-specific metabolic pathway hijacked from one of the most ATP-intensive processes in the animal kingdom: sperm motility.
Tue Sep 29 00:00:00 UTC 2020
Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease.
The Journal of biological chemistry
Zhong X,Jagarlapudi S,Weng Y,Ly M,Rouse JC,McClure K,Ishino T,Zhang Y,Sousa E,Cohen J,Tzvetkova B,Cote K,Scarcelli JJ,Johnson K,Palandra J,Apgar JR,Yaddanapudi S,Gonzalez-Villalobos RA,Opsahl AC,Lam K,Yao Q,Duan W,Sievers A,Zhou J,Ferguson D,D'Antona A,Zollner R,Zhu HL,Kriz R,Lin L,Clerin V
R960-25 was used in Western Blotting to explore the structure-function relationship and post-translational modifications of sKlotho variants to guide the future design of sKlotho-based therapeutics.
Fri Mar 06 00:00:00 UTC 2020
Discovery of EMRE in fungi resolves the true evolutionary history of the mitochondrial calcium uniporter.
Nature communications
Pittis AA,Goh V,Cebrian-Serrano A,Wettmarshausen J,Perocchi F,Gabaldón T
R960-25 was used in Western Blotting to undertake a comprehensive evolutionary analysis of the mt-Ca2+ uniporter.
Wed Aug 12 00:00:00 UTC 2020
The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice.
Nature communications
Yesbolatova A,Saito Y,Kitamoto N,Makino-Itou H,Ajima R,Nakano R,Nakaoka H,Fukui K,Gamo K,Tominari Y,Takeuchi H,Saga Y,Hayashi KI,Kanemaki MT
R960-25 was used in Flow Cytometry to develop the auxin-inducible degron 2 system for improved protein knockdown function in living cells.
Wed Nov 11 00:00:00 UTC 2020
Mps2 links Csm4 and Mps3 to form a telomere-associated LINC complex in budding yeast.
Life science alliance
Fan J,Jin H,Koch BA,Yu HG
R960-25 was used in Immunocytochemistry-immunoflourescence to study the interaction between KASH-like proteins Csm4 and Mps3 and the influence of SUN domain protein Mps3 on this pair.
Tue Dec 01 00:00:00 UTC 2020
Long noncoding RNA PAHAL modulates locust behavioural plasticity through the feedback regulation of dopamine biosynthesis.
PLoS genetics
Zhang X,Xu Y,Chen B,Kang L
R960-25 was used in RNA Immunoprecipitation to report the regulatory mechanism of the nuclear-enriched lncRNA PAHAL for dopamine biosynthesis and behavioural adjustment in migratory locusts (Locusta migratoria).
Wed Apr 01 00:00:00 UTC 2020
Back-Pyrophosphorylation Assay to Detect In Vivo InsP7-Dependent Protein Pyrophosphorylation in Mammalian Cells.
Methods in molecular biology (Clifton, N.J.)
Chanduri M,Bhandari R
R960-25 was used in Immunoprecipitation to identify an InsP7 target protein and confirm its endogenous pyrophosphorylation status.
Mon Jan 18 00:00:00 UTC 2021
Mechanism of actin N-terminal acetylation.
Science advances
Rebowski G,Boczkowska M,Drazic A,Ree R,Goris M,Arnesen T,Dominguez R
R960CUS was used in Immunoprecipitation to determine the structural and functional features of actin N-terminal acetylation.
Wed Apr 01 00:00:00 UTC 2020
Mechanism of actin N-terminal acetylation.
Science advances
Rebowski G,Boczkowska M,Drazic A,Ree R,Goris M,Arnesen T,Dominguez R
R960CUS was used in Immunoprecipitation to determine the structural and functional features of actin N-terminal acetylation.
Wed Apr 01 00:00:00 UTC 2020
Nephronectin promotes breast cancer brain metastatic colonization via its integrin-binding domains.
Scientific reports
Magnussen SN,Toraskar J,Wilhelm I,Hasko J,Figenschau SL,Molnar J,Seppola M,Steigen SE,Steigedal TS,Hadler-Olsen E,Krizbai IA,Svineng G
R960-25 was used in Western Blotting to demonstrate a role for the extracellular matrix protein nephronectin (NPNT) in promoting experimental breast cancer brain metastasis.
Wed Jul 22 00:00:00 UTC 2020
The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer.
eLife
Gibbs ZA,Reza LC,Cheng CC,Westcott JM,McGlynn K,Whitehurst AW
R960-25 was used in Proximity Ligation Assay (PLA) to report that ZNF165 associates with SMAD3 to modulate transcription of transforming growth factor b (TGFb)- dependent genes and thereby promote growth and survival of human triple-negative breast cancer cells.
Tue Jun 09 00:00:00 UTC 2020
Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury.
Nature
Zhou HL,Zhang R,Anand P,Stomberski CT,Qian Z,Hausladen A,Wang L,Rhee EP,Parikh SM,Karumanchi SA,Stamler JS
R960-25 was used in Western Blotting to identify a physiological function of the SNO-CoA-SCoR system in mammals, describe new regulation of renal metabolism and of PKM2 in differentiated tissues, and offer a novel perspective on kidney injury with therapeutic implications.
Tue Jan 01 00:00:00 UTC 2019
Excessive Cell Growth Causes Cytoplasm Dilution And Contributes to Senescence.
Cell
Neurohr GE,Terry RL,Lengefeld J,Bonney M,Brittingham GP,Moretto F,Miettinen TP,Vaites LP,Soares LM,Paulo JA,Harper JW,Buratowski S,Manalis S,van Werven FJ,Holt LJ,Amon A
R960-25 was used in Chromatin immunoprecipitation to investigate the effect of growing budding yeast and primary mammalian cells beyond a certain size, on gene induction, cell-cycle progression, and cell signalling.
Thu Feb 21 00:00:00 UTC 2019
Deubiquitinase USP10 regulates Notch signaling in the endothelium.
Science (New York, N.Y.)
Lim R,Sugino T,Nolte H,Andrade J,Zimmermann B,Shi C,Doddaballapur A,Ong YT,Wilhelm K,Fasse JWD,Ernst A,Kaulich M,Husnjak K,Boettger T,Guenther S,Braun T,Krüger M,Benedito R,Dikic I,Potente M
R960-25 was used in Western Blot to conclude that USP10 functions as an NICD1 deubiquitinase that fine-tunes endothelial Notch responses during angiogenic sprouting.
Fri Apr 12 00:00:00 UTC 2019
GAPDH Overexpression in the T Cell Lineage Promotes Angioimmunoblastic T Cell Lymphoma through an NF-κB-Dependent Mechanism.
Cancer cell
Mondragón L,Mhaidly R,De Donatis GM,Tosolini M,Dao P,Martin AR,Pons C,Chiche J,Jacquin M,Imbert V,Proïcs E,Boyer L,Doye A,Luciano F,Neels JG,Coutant F,Fabien N,Sormani L,Rubio-Patiño C,Bossowski JP,Muller F,Marchetti S,Villa E,Peyron JF,Gaulard P,Lemonnier F,Asnafi V,Genestier L,Benhida R,Fournié JJ,Passeron T,Ricci JE,Verhoeyen E
R960-25 was used in Western Blot, Immunoprecipitation to suggest the therapeutic potential of targeting NF-κB signaling in AITL and provide a model for future AITL therapeutic investigations.
Mon Sep 16 00:00:00 UTC 2019
GAPDH Overexpression in the T Cell Lineage Promotes Angioimmunoblastic T Cell Lymphoma through an NF-κB-Dependent Mechanism.
Cancer cell
Mondragón L,Mhaidly R,De Donatis GM,Tosolini M,Dao P,Martin AR,Pons C,Chiche J,Jacquin M,Imbert V,Proïcs E,Boyer L,Doye A,Luciano F,Neels JG,Coutant F,Fabien N,Sormani L,Rubio-Patiño C,Bossowski JP,Muller F,Marchetti S,Villa E,Peyron JF,Gaulard P,Lemonnier F,Asnafi V,Genestier L,Benhida R,Fournié JJ,Passeron T,Ricci JE,Verhoeyen E
R960-25 was used in Western Blot, Immunoprecipitation to suggest the therapeutic potential of targeting NF-κB signaling in AITL and provide a model for future AITL therapeutic investigations.
Mon Sep 16 00:00:00 UTC 2019
ALOX12 is required for p53-mediated tumour suppression through a distinct ferroptosis pathway.
Nature cell biology
Chu B,Kon N,Chen D,Li T,Liu T,Jiang L,Song S,Tavana O,Gu W
R960-25 was used in Western Blotting to identify an ALOLX12-mediated, ACSL4-independent ferroptosis pathway that is critical for p53-dependent tumor suppression.
Wed May 01 00:00:00 UTC 2019
Translational reprogramming marks adaptation to asparagine restriction in cancer.
Nature cell biology
Pathria G,Lee JS,Hasnis E,Tandoc K,Scott DA,Verma S,Feng Y,Larue L,Sahu AD,Topisirovic I,Ruppin E,Ronai ZA
R960-25 was used in Western Blotting to demonstrate a role of translational reprogramming in the survival of asparagine-restricted cancer cells.
Sun Dec 01 00:00:00 UTC 2019
Distinct Classes of Chromatin Loops Revealed by Deletion of an RNA-Binding Region in CTCF.
Molecular cell
Hansen AS,Hsieh TS,Cattoglio C,Pustova I,Saldaña-Meyer R,Reinberg D,Darzacq X,Tjian R
R960-25 was used in Western Blotting to demonstrate RBRi-independent and RBRi-dependent loops are two distinct classes of CTCF loops.
Thu Nov 07 00:00:00 UTC 2019
Splicing events in the control of genome integrity: role of SLU7 and truncated SRSF3 proteins.
Nucleic acids research
Jiménez M,Urtasun R,Elizalde M,Azkona M,Latasa MU,Uriarte I,Arechederra M,Alignani D,Bárcena-Varela M,Álvarez-Sola G,Colyn L,Santamaría E,Sangro B,Rodriguez-Ortigosa C,Fernández-Barrena MG,Ávila MA,Berasain C
R960-25 was used in Western Blotting to identify the splicing regulator SLU7 as a key mediator of genome stability.
Tue Apr 23 00:00:00 UTC 2019
A conserved genetic interaction between Spt6 and Set2 regulates H3K36 methylation.
Nucleic acids research
Gopalakrishnan R,Marr SK,Kingston RE,Winston F
R960-25 was used in Western Blotting to study the nature of the requirement for Spt6 in H3K26 methylation in Saccharomyces cerevisiae.
Tue May 07 00:00:00 UTC 2019
The structure of human EXD2 reveals a chimeric 3' to 5' exonuclease domain that discriminates substrates via metal coordination.
Nucleic acids research
Park J,Lee SY,Jeong H,Kang MG,Van Haute L,Minczuk M,Seo JK,Jun Y,Myung K,Rhee HW,Lee C
R960-25 was used in Immunocytochemistry-immunoflourescence to determine the function of EXD2.
Fri Jul 26 00:00:00 UTC 2019
Rad5 dysregulation drives hyperactive recombination at replication forks resulting in cisplatin sensitivity and genome instability.
Nucleic acids research
Bryant EE,Šunjevarić I,Berchowitz L,Rothstein R,Reid RJD
R960-25 was used in Western blotting following immunoprecipitation to model HLTF dysregulation through the functionally conserved Saccharomyce cerevisiae ortholog, RAD5.
Thu Sep 26 00:00:00 UTC 2019
Plug-and-Play Protein Modification Using Homology-Independent Universal Genome Engineering.
Neuron
Gao Y,Hisey E,Bradshaw TWA,Erata E,Brown WE,Courtland JL,Uezu A,Xiang Y,Diao Y,Soderling SH
R960-25 was used in Immunohistochemistry to demonstrate a CRISPR-Cas9-based homology-independent universal genome engineering (HiUGE) method for endogenous protein manipulation that is straightforward, scalable, and highly flexible in terms of genomic target and application.
Wed Aug 21 00:00:00 UTC 2019
Plug-and-Play Protein Modification Using Homology-Independent Universal Genome Engineering.
Neuron
Gao Y,Hisey E,Bradshaw TWA,Erata E,Brown WE,Courtland JL,Uezu A,Xiang Y,Diao Y,Soderling SH
R960-25 was used in Immunohistochemistry to demonstrate a CRISPR-Cas9-based homology-independent universal genome engineering (HiUGE) method for endogenous protein manipulation that is straightforward, scalable, and highly flexible in terms of genomic target and application.
Wed Aug 21 00:00:00 UTC 2019
SETD5 Regulates Chromatin Methylation State and Preserves Global Transcriptional Fidelity during Brain Development and Neuronal Wiring.
Neuron
Sessa A,Fagnocchi L,Mastrototaro G,Massimino L,Zaghi M,Indrigo M,Cattaneo S,Martini D,Gabellini C,Pucci C,Fasciani A,Belli R,Taverna S,Andreazzoli M,Zippo A,Broccoli V
R960-25 was used in Chromatin immunoprecipitation to show that Setd5 haploinsufficiency impairs the proliferative dynamics of neural progenitors and synaptic wiring of neurons, ultimately resulting in behavioral deficits in mice.
Wed Oct 23 00:00:00 UTC 2019
The herpesviral antagonist m152 reveals differential activation of STING-dependent IRF and NF-κB signaling and STING's dual role during MCMV infection.
The EMBO journal
Stempel M,Chan B,Juranić Lisnić V,Krmpotić A,Hartung J,Paludan SR,Füllbrunn N,Lemmermann NA,Brinkmann MM
R960-25 was used in Immunoprecipitation to show that MCMV has evolved a mechanism to specifically antagonize the STING-mediated antiviral IFN response, while preserving its pro-viral NF-κB response, providing an advantage in the establishment of an infection.
Fri Mar 01 00:00:00 UTC 2019
ERp18 regulates activation of ATF6α during unfolded protein response.
The EMBO journal
Oka OB,van Lith M,Rudolf J,Tungkum W,Pringle MA,Bulleid NJ
R960-25 was used in Western Blotting to demonstrate that ERp18 monitors ATF6α ER quality control to ensure optimal processing following trafficking to the Golgi.
Thu Aug 01 00:00:00 UTC 2019
Disease-associated tau impairs mitophagy by inhibiting Parkin translocation to mitochondria.
The EMBO journal
Cummins N,Tweedie A,Zuryn S,Bertran-Gonzalez J,Götz J
R960-25 was used in Proximity Ligation Assay to study the mechanisms by which disease-associated tau impairs mitophagy.
Fri Feb 01 00:00:00 UTC 2019
Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies.
Autophagy
Haidar M,Asselbergh B,Adriaenssens E,De Winter V,Timmermans JP,Auer-Grumbach M,Juneja M,Timmerman V
R960-25 was used in Western Blotting to study the effect of mutations in HSPB1 on macroautophagic/autophagic flux.
Sat Jun 01 00:00:00 UTC 2019
The Pro-Oncogenic Adaptor CIN85 Acts as an Inhibitory Binding Partner of Hypoxia-Inducible Factor Prolyl Hydroxylase 2.
Cancer research
Kozlova N,Mennerich D,Samoylenko A,Dimova EY,Koivunen P,Biterova E,Richter K,Hassinen A,Kellokumpu S,Manninen A,Miinalainen I,Glumoff V,Ruddock L,Drobot LB,Kietzmann T
R960-25 was used in Western Blotting to uncover an essential survival function in tumor cells by linking growth factor adaptors with hypoxia signaling.
Thu Aug 15 00:00:00 UTC 2019
N6-Methylation of Adenosine of FZD10 mRNA Contributes to PARP Inhibitor Resistance.
Cancer research
Fukumoto T,Zhu H,Nacarelli T,Karakashev S,Fatkhutdinov N,Wu S,Liu P,Kossenkov AV,Showe LC,Jean S,Zhang L,Zhang R
R960-25 was used in Western Blotting to show that m6A modification of FZD10 mRNA contributes to PARP inhibitor resistance by upregulating the Wnt/β-catenin pathway in BRCA-mutated epithelial ovarian cancer cells.
Sat Jun 01 00:00:00 UTC 2019
Termination of pre-mRNA splicing requires that the ATPase and RNA unwindase Prp43p acts on the catalytic snRNA U6.
Genes & development
Toroney R,Nielsen KH,Staley JP
R960-25 was used in Immunoprecipitation to indicate a mechanism of splicing termination in which Prp43p translocates along U6 from the 3' end to disassemble the spliceosome and thereby release suboptimal substrates or excised introns.
Fri Nov 01 00:00:00 UTC 2019
Fibroblast growth factor receptor influences primary cilium length through an interaction with intestinal cell kinase.
Proceedings of the National Academy of Sciences of the United States of America
Kunova Bosakova M,Nita A,Gregor T,Varecha M,Gudernova I,Fafilek B,Barta T,Basheer N,Abraham SP,Balek L,Tomanova M,Fialova Kucerova J,Bosak J,Potesil D,Zieba J,Song J,Konik P,Park S,Duran I,Zdrahal Z,Smajs D,Jansen G,Fu Z,Ko HW,Hampl A,Trantirek L,Krakow D,Krejci P
R960-25 was used in Western Blotting to delineate a mechanism by which fibroblast growth factor controls primary cilia.
Tue Mar 05 00:00:00 UTC 2019
Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking.
Cell death and differentiation
Fauster A,Rebsamen M,Willmann KL,César-Razquin A,Girardi E,Bigenzahn JW,Schischlik F,Scorzoni S,Bruckner M,Konecka J,Hörmann K,Heinz LX,Boztug K,Superti-Furga G
R960-25 was used in Western Blotting to investigate the genetic basis underlying necroptotic cell death.
Sat Jun 01 00:00:00 UTC 2019
Interactions between lysyl oxidases and ADAMTS proteins suggest a novel crosstalk between two extracellular matrix families.
Matrix biology : journal of the International Society for Matrix Biology
Aviram R,Zaffryar-Eilot S,Hubmacher D,Grunwald H,Mäki JM,Myllyharju J,Apte SS,Hasson P
R960-25 was used in Western Blotting to demonstrate that several members of the LOX and ADAMTS families interact with one another.
Tue Jan 01 00:00:00 UTC 2019
The Candida albicans biofilm gene circuit modulated at the chromatin level by a recent molecular histone innovation.
PLoS biology
Rai LS,Singha R,Sanchez H,Chakraborty T,Chand B,Bachellier-Bassi S,Chowdhury S,d'Enfert C,Andes DR,Sanyal K
R960-25 was used in Western Blotting to show that differential gene expression patterns are modulated by the relative occupancy of the specific histone H3 type at the chromatin level.
Thu Aug 01 00:00:00 UTC 2019
ATF7IP regulates SETDB1 nuclear localization and increases its ubiquitination.
EMBO reports
Tsusaka T,Shimura C,Shinkai Y
R960-25 was used in Western Blotting to determine how ATF7IP can regulate the histone methyltransferase activity of SETDB1 accompanied by its nuclear translocation.
Thu Dec 05 00:00:00 UTC 2019
TRiC/CCT chaperonins are essential for organ growth by interacting with insulin/TOR signaling in Drosophila.
Oncogene
Kim AR,Choi KW
R960-25 was used in Western Blotting to study the role of the chaperonin containing TCP-1 complex in Drosophila organ growth.
Sat Jun 01 00:00:00 UTC 2019
The anaphase-promoting complex regulates the degradation of the inner nuclear membrane protein Mps3.
The Journal of cell biology
Koch BA,Jin H,Tomko RJ,Yu HG
R960-25 was used in Western Blot to show that the anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase, regulates the degradation of Mps3, a conserved integral protein of the inner nuclear membrane.
Mon Mar 04 00:00:00 UTC 2019
Developmental regulation of an organelle tether coordinates mitochondrial remodeling in meiosis.
The Journal of cell biology
Sawyer EM,Joshi PR,Jorgensen V,Yunus J,Berchowitz LE,Ünal E
R960-25 was used in Western Blotting to study the developmental control of organelle morphogenesis.
Mon Feb 04 00:00:00 UTC 2019
The RNA export factor Mex67 functions as a mobile nucleoporin.
The Journal of cell biology
Derrer CP,Mancini R,Vallotton P,Huet S,Weis K,Dultz E
R960-25 was used in Western Blotting to use quantitative fluorescence microscopy techniques in live budding yeast cells to investigate how Mex67 facilitates mRNA export.
Mon Dec 02 00:00:00 UTC 2019
Cul4 ubiquitin ligase cofactor DCAF12 promotes neurotransmitter release and homeostatic plasticity.
The Journal of cell biology
Patrón LA,Nagatomo K,Eves DT,Imad M,Young K,Torvund M,Guo X,Rogers GC,Zinsmaier KE
R960-25 was used in Western Blot to genetically characterise the synaptic role of the Drosophila homologue of human DCAF12, a putative cofactor of Cullin4 (Cul4) ubiquitin ligase complexes.
Mon Mar 04 00:00:00 UTC 2019
PTEN reduces endosomal PtdIns(4,5)P2 in a phosphatase-independent manner via a PLC pathway.
The Journal of cell biology
Mondin VE,Ben El Kadhi K,Cauvin C,Jackson-Crawford A,Bélanger E,Decelle B,Salomon R,Lowe M,Echard A,Carréno S
R960-25 was used in Immunocytochemistry-Immunofluorescence to study the function of the tumor supressor, PTEN, in Lowe syndrome.
Mon Jul 01 00:00:00 UTC 2019
Mutations in RHOT1 Disrupt Endoplasmic Reticulum-Mitochondria Contact Sites Interfering with Calcium Homeostasis and Mitochondrial Dynamics in Parkinson's Disease.
Antioxidants & redox signaling
Grossmann D,Berenguer-Escuder C,Bellet ME,Scheibner D,Bohler J,Massart F,Rapaport D,Skupin A,Fouquier d'Hérouël A,Sharma M,Ghelfi J,Raković A,Lichtner P,Antony P,Glaab E,May P,Dimmer KS,Fitzgerald JC,Grünewald A,Krüger R
R960-25 was used in Western Blot to explore the genetic and functional contribution of RHOT1 mutations to Parkinson’s disease in patient-derived cellular models.
Sun Dec 01 00:00:00 UTC 2019
Astrocytic expression of the chaperone DNAJB6 results in non-cell autonomous protection in Huntington's disease.
Neurobiology of disease
Bason M,Meister-Broekema M,Alberts N,Dijkers P,Bergink S,Sibon OCM,Kampinga HH
R960-25 was used in Western Blot, Immunohistochemistry to provide direct evidence for a non-cell autonomous protective role of astrocytes in PolyQ diseases.
Mon Apr 01 00:00:00 UTC 2019
Astrocytic expression of the chaperone DNAJB6 results in non-cell autonomous protection in Huntington's disease.
Neurobiology of disease
Bason M,Meister-Broekema M,Alberts N,Dijkers P,Bergink S,Sibon OCM,Kampinga HH
R960-25 was used in Western Blot, Immunohistochemistry to provide direct evidence for a non-cell autonomous protective role of astrocytes in PolyQ diseases.
Mon Apr 01 00:00:00 UTC 2019
The Interferon-Inducible Proteoglycan Testican-2/SPOCK2 Functions as a Protective Barrier against Virus Infection of Lung Epithelial Cells.
Journal of virology
Ahn N,Kim WJ,Kim N,Park HW,Lee SW,Yoo JY
R960-25 was used in Western Blotting to demonstrate that testican-2/SPOCK2 effectively protects lung epithelial cells from influenza virus infection.
Tue Oct 15 00:00:00 UTC 2019
The Interferon-Inducible Proteoglycan Testican-2/SPOCK2 Functions as a Protective Barrier against Virus Infection of Lung Epithelial Cells.
Journal of virology
Ahn N,Kim WJ,Kim N,Park HW,Lee SW,Yoo JY
R960-25 was used in Western Blotting to demonstrate that testican-2/SPOCK2 effectively protects lung epithelial cells from influenza virus infection.
Tue Oct 15 00:00:00 UTC 2019
Analysis of Coronavirus Temperature-Sensitive Mutants Reveals an Interplay between the Macrodomain and Papain-Like Protease Impacting Replication and Pathogenesis.
Journal of virology
Deng X,Mettelman RC,O'Brien A,Thompson JA,O'Brien TE,Baker SC
R960-25 was used in Immunoprecipitation to reveal an unanticipated interplay between the macrodomain and the PLP2 domain that is important for replication and antagonizing the host innate immune response, which may provide new therapeutic opportunities to treat CoV disease.
Sat Jun 15 00:00:00 UTC 2019
The Tumor Suppressor FBW7 and the Vitamin D Receptor Are Mutual Cofactors in Protein Turnover and Transcriptional Regulation.
Molecular cancer research : MCR
Salehi-Tabar R,Memari B,Wong H,Dimitrov V,Rochel N,White JH
R960-25 was used in Chromatin immunoprecipitation to determine whether FBW7 and the vitamin D receptor control each other's function as regulators of protein turnover and gene transcription, respectively.
Fri Mar 01 00:00:00 UTC 2019
Down-Regulation of miRNA-708 Promotes Aberrant Calcium Signaling by Targeting Neuronatin in a Mouse Model of Angelman Syndrome.
Frontiers in molecular neuroscience
Vatsa N,Kumar V,Singh BK,Kumar SS,Sharma A,Jana NR
R960-25 was used in Western Blotting to study the role of miRNA in Angelman syndrome pathogenesis.
Thu Oct 01 00:00:00 UTC 2020
Enhancer of Polycomb and the Tip60 complex repress hematological tumor initiation by negatively regulating JAK/STAT pathway activity.
Disease models & mechanisms
Bailetti AA,Negrón-Piñeiro LJ,Dhruva V,Harsh S,Lu S,Bosula A,Bach EA
R960-25 was used in Immunoprecipitation to indicate that E(Pc) works as a tumor suppressor by attenuating Hop protein expression and ultimately JAK/STAT signaling.
Thu May 30 00:00:00 UTC 2019
N-Glycan-calnexin interactions in human factor VII secretion and deficiency.
The international journal of biochemistry & cell biology
Wang H,Wang L,Li S,Dong N,Wu Q
R960-25 was used in Western Blotting to show human FVII requires N-glycosylation at N360 in the protease domain in order to be secreted.
Thu Aug 01 00:00:00 UTC 2019
ZC3H12B/MCPIP2, a new active member of the ZC3H12 family.
RNA (New York, N.Y.)
Wawro M,Wawro K,Kochan J,Solecka A,Sowinska W,Lichawska-Cieslar A,Jura J,Kasza A
R960-25 was used in Western Blotting to characterize the properties of the ZC3H12B protein.
Mon Jul 01 00:00:00 UTC 2019
A novel cell-based sensor detecting the activity of individual basic proprotein convertases.
The FEBS journal
Löw K,Hardes K,Fedeli C,Seidah NG,Constam DB,Pasquato A,Steinmetzer T,Roulin A,Kunz S
R960-25 was used in Immunocytochemistry to report basic PC sensor (BPCS), a novel cell-based molecular sensor that allows rapid screening of candidate inhibitors and their selectivity toward individual basic PCs within mammalian cells.
Fri Nov 01 00:00:00 UTC 2019
Gasdermin D Drives the Nonexosomal Secretion of Galectin-3, an Insulin Signal Antagonist.
Journal of immunology (Baltimore, Md. : 1950)
Chen Y,Wang H,Shen J,Deng R,Yao X,Guo Q,Lu A,Sun B,Zhang Y,Meng G
R960-25 was used in Western Blotting to identify galectin-3 as an effector protein of the NLRP3 inflammasome.
Fri Nov 15 00:00:00 UTC 2019
Dysregulation of NRAP degradation by KLHL41 contributes to pathophysiology in nemaline myopathy.
Human molecular genetics
Jirka C,Pak JH,Grosgogeat CA,Marchetii MM,Gupta VA
R960CUS was used in Western Blotting to conclude that defects in KLHL41-mediated ubiquitination of sarcomeric proteins contribute to structural and functional deficits in skeletal muscle, and hence contributes to nemaline myopathy pathophysiology.
Thu Aug 01 00:00:00 UTC 2019
FANCD2 Binding to H4K20me2 via a Methyl-Binding Domain Is Essential for Efficient DNA Cross-Link Repair.
Molecular and cellular biology
Paquin KL,Mamrak NE,Garzon JL,Cantres-Velez JA,Azzinaro PA,Vuono EA,Lima KE,Camberg JL,Howlett NG
R960-25 was used in Flow cytometry/Cell sorting to identify and characterise a FANCD2 histone-binding domain (HBD) and embedded methylated lysine-binding domain (MBD) and demonstrate binding specificity for H4K20me2.
Thu Aug 01 00:00:00 UTC 2019
Directed Evolution of Split APEX2 Peroxidase.
ACS chemical biology
Han Y,Branon TC,Martell JD,Boassa D,Shechner D,Ellisman MH,Ting A
R960-25 was used in Western Blotting to demonstrate sAPEX reconstitution in the mammalian cytosol, on engineered RNA motifs within a non-coding RNA scaffold, and at mitochondria-endoplasmic reticulum contact sites.
Fri Apr 19 00:00:00 UTC 2019
Integration of Self and Non-self Recognition Modulates Asexual Cell-to-Cell Communication in Neurospora crassa.
Genetics
Fischer MS,Jonkers W,Glass NL
R960-25 was used in Western Blotting to show that HAM-11 functions in parallel with the DOC-1 and DOC-2 proteins to regulate the activity of the MAPK signaling complex.
Mon Apr 01 00:00:00 UTC 2019
Tel1 Activation by the MRX Complex Is Sufficient for Telomere Length Regulation but Not for the DNA Damage Response in Saccharomyces cerevisiae.
Genetics
Keener R,Connelly CJ,Greider CW
R960-25 was used in Western Blotting to provide evidence that telomere length regulation differs from the DNA damage response in both the Tel1 and Mec1 pathways.
Sun Dec 01 00:00:00 UTC 2019
Generating and evaluating type I interferon receptor-deficient and feline TMPRSS2-expressing cells for propagating serotype I feline infectious peritonitis virus.
Virology
Mettelman RC,O'Brien A,Whittaker GR,Baker SC
R960-25 was used in Western Blotting to study the replication kinetics of a serotype I feline infectious peritonitis virus (FIPV) within two feline cell lines.
Fri Nov 01 00:00:00 UTC 2019
Minimal effects of spargel (PGC-1) overexpression in a Drosophila mitochondrial disease model.
Biology open
George J,Jacobs HT
R960-25 was used in Western Blotting to analyse the effects of srl overexpression on phenotype and on gene expression in tko25t, a recessive bang-sensitive mutant with a global defect in oxidative phosphorylation, resulting from a deficiency of mitochondrial protein synthesis.
Fri Jul 19 00:00:00 UTC 2019
Identification of epithelial-specific ETS-1 (ESE-1) as a tumor suppressor and molecular target of green tea compound, EGCG.
Molecular carcinogenesis
Ha T,Lee J,Lou Z,Lee BS,Kim CH,Lee SH
R960-25 was used in In vitro experiments to study the effects of epithelial specific ETS-1 (ESE-1) on cancer development and progression to investigate whether ESE-1 is a tumor suppressor and to identify dietary anti-cancer compound to activate ESE-1 expression in human colon cancer model. To identify epigallocatechin-3-gallate (EGCG) that acts as a transcriptional activator of ESE-1 in human colon cancer cells.
Sat Jun 01 00:00:00 UTC 2019
The conservation and functionality of the oxygen-sensing enzyme Factor Inhibiting HIF (FIH) in non-vertebrates.
PloS one
Hampton-Smith RJ,Davenport BA,Nagarajan Y,Peet DJ
R960-25 was used in Western Blotting to determine the conservation and functional importance of Factor Inhibiting HIF in metazoa.
Wed Jan 08 00:00:00 UTC 2020
LMO7 deficiency reveals the significance of the cuticular plate for hearing function.
Nature communications
Du TT,Dewey JB,Wagner EL,Cui R,Heo J,Park JJ,Francis SP,Perez-Reyes E,Guillot SJ,Sherman NE,Xu W,Oghalai JS,Kachar B,Shin JB
R960-25 was used in Immunocytochemistry to study the function of the cuticular plate in hearing function, especially the significance of its integrity for long-term maintenance of hair cell mechanotransduction.
Fri Mar 08 00:00:00 UTC 2019
Dzip1 and Fam92 form a ciliary transition zone complex with cell type specific roles in Drosophila.
eLife
Lapart JA,Gottardo M,Cortier E,Duteyrat JL,Augière C,Mangé A,Jerber J,Solassol J,Gopalakrishnan J,Thomas J,Durand B
R960-25 was used in Western Blotting to demonstrate a regulatory role for Dzip1 and Fam92 in mediating membrane/basal-body interactions and show that these interactions exhibit cell type specific functions in basal-body maturation and transition zone organisation.
Tue Dec 10 00:00:00 UTC 2019
Severe fever with thrombocytopenia syndrome phlebovirus non-structural protein activates TPL2 signalling pathway for viral immunopathogenesis.
Nature microbiology
Choi Y,Park SJ,Sun Y,Yoo JS,Pudupakam RS,Foo SS,Shin WJ,Chen SB,Tsichlis PN,Lee WJ,Lee JS,Li W,Brennan B,Choi YK,Jung JU
R960-25 was used in Western Blot to demonstrate that SFTSV-NSs (severe fever with thrombocytopenia syndrome phlebovirus-non-structural protein) targets the tumour progression locus 2 (TPL2)-A20-binding inhibitor of NF-κB activation 2 (ABIN2)-p105 complex to induce the expression of interleukin-10 (IL-10) for viral pathogenesis.
Fri Mar 01 00:00:00 UTC 2019
Cholesterol increases protein levels of the E3 ligase MARCH6 and thereby stimulates protein degradation.
The Journal of biological chemistry
Sharpe LJ,Howe V,Scott NA,Luu W,Phan L,Berk JM,Hochstrasser M,Brown AJ
R960-25 was used in Western Blot to highlight an important new role for cholesterol in controlling levels of proteins, extending the known repertoire of cholesterol homeostasis players.
Fri Feb 15 00:00:00 UTC 2019
Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides.
eLife
Kol M,Williams B,Toombs-Ruane H,Franquelim HG,Korneev S,Schroeer C,Schwille P,Trauner D,Holthuis JC,Frank JA
R960-25 was used in Western Blotting to study the applications of clickable, azobenzene-containing ceramides as photoswitchable metabolic substrates to exert optical control over sphingolipid production in cells.
Tue Feb 05 00:00:00 UTC 2019
Simultaneous targeting of linked loci in mouse embryos using base editing.
Scientific reports
Lee HK,Willi M,Smith HE,Miller SM,Liu DR,Liu C,Hennighausen L
R960-25 was used in Chromatin immunoprecipitation to assess the applications of base editing in introducing linked mutations simultaneously into the mammalian germline.
Thu Feb 07 00:00:00 UTC 2019
Molecular basis of synaptic specificity by immunoglobulin superfamily receptors in Drosophila.
eLife
Cheng S,Ashley J,Kurleto JD,Lobb-Rabe M,Park YJ,Carrillo RA,Özkan E
R960-25 was used in Immunohistochemistry-immunofluorescence to study the molecular basis of specificity in the immunoglobulin superfamily proteins in Drosophila mediated cellular adhesions and neuronal connectivity.
Mon Jan 28 00:00:00 UTC 2019
A Sir2-regulated locus control region in the recombination enhancer of Saccharomyces cerevisiae specifies chromosome III structure.
PLoS genetics
Li M,Fine RD,Dinda M,Bekiranov S,Smith JS
R960-25 was used in Western Blotting to demonstrate that Sir2 and the condensin complex are recruited to the RE exclusively in MATa cells, specifically to the promoter of a small gene within the right half of the RE known as RDT1.
Thu Aug 01 00:00:00 UTC 2019
Proteotoxicity from aberrant ribosome biogenesis compromises cell fitness.
eLife
Tye BW,Commins N,Ryazanova LV,Wühr M,Springer M,Pincus D,Churchman LS
R960-25 was used in Western Blotting to model perturbations in Saccharomyces cerevisiae and show that challenges to ribosome biogenesis result in acute loss of proteostasis.
Thu Mar 07 00:00:00 UTC 2019
In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation.
Nature communications
Spence EF,Dube S,Uezu A,Locke M,Soderblom EJ,Soderling SH
R960-25 was used in Western Blotting to study the molecular mechanisms that underlie excitatory synaptogenesis.
Wed Jan 23 00:00:00 UTC 2019
Protein phosphatase 2A interacts with Verthandi/Rad21 to regulate mitosis and organ development in Drosophila.
Scientific reports
Kim LH,Hong ST,Choi KW
R960-25 was used in Western Blotting to provide evidence that PP2A is required for mitosis and wing growth by regulating the Vtd level through the proteasomal pathway.
Mon May 20 00:00:00 UTC 2019
Cross-linking, Immunoprecipitation and Proteomic Analysis to Identify Interacting Proteins in Cultured Cells.
Bio-protocol
Wang H,He M,Willard B,Wu Q
R960-25 was used in Immunoprecipitation to describe a protocol of protein cross-linking, co-immunoprecipitation, and proteomic analysis, which was used to identify endoplasmic reticulum (ER) chaperones critical for the folding and ER exiting of N-glycosylated serine proteases in human embryonic kidney (HEK) 293 cells.
Wed Jun 05 00:00:00 UTC 2019
Evolution of the rodent Trim5 cluster is marked by divergent paralogous expansions and independent acquisitions of TrimCyp fusions.
Scientific reports
Boso G,Shaffer E,Liu Q,Cavanna K,Buckler-White A,Kozak CA
R960-25 was used in Western Blotting to describe a complex pattern of differential gene amplification in the Trim5 cluster of rodents and identify the first functional TrimCyp fusion gene outside of primates and tree shrews.
Fri Aug 02 00:00:00 UTC 2019
Chromatin Decondensation by FOXP2 Promotes Human Neuron Maturation and Expression of Neurodevelopmental Disease Genes.
Cell reports
Hickey SL,Berto S,Konopka G
R960-25 was used in Immunocytochemistry-immunoflourescence to study the mechanisms by which FOXP2 exerts regulatory control over targets during neuronal maturation.
Tue May 07 00:00:00 UTC 2019
Regulation of subcellular dendritic synapse specificity by axon guidance cues.
eLife
Sales EC,Heckman EL,Warren TL,Doe CQ
R960-25 was used in Immunohistochemistry-immunofluorescence to study the regulation of subcellular dendritic synapse specificity by axon guidance cues.
Tue Apr 23 00:00:00 UTC 2019
CRL4 regulates recombination and synaptonemal complex aggregation in the Caenorhabditis elegans germline.
PLoS genetics
Alleva B,Clausen S,Koury E,Hefel A,Smolikove S
R960-25 was used in Western Blotting to study functions of Cullin RING E3 ubiquitin ligase 4 in C. elegans meiosis.
Fri Nov 01 00:00:00 UTC 2019
Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions.
eLife
Nobre LV,Nightingale K,Ravenhill BJ,Antrobus R,Soday L,Nichols J,Davies JA,Seirafian S,Wang EC,Davison AJ,Wilkinson GW,Stanton RJ,Huttlin EL,Weekes MP
R960-25 was used in Western Blot to find that non-canonical ORFL147C protein interacting with elements of the mRNA splicing machinery, and a mutational study suggested its importance in viral replication.
Tue Dec 24 00:00:00 UTC 2019
A cryptic hydrophobic pocket in the polo-box domain of the polo-like kinase PLK1 regulates substrate recognition and mitotic chromosome segregation.
Scientific reports
Sharma P,Mahen R,Rossmann M,Stokes JE,Hardwick B,Huggins DJ,Emery A,Kunciw DL,Hyvönen M,Spring DR,McKenzie GJ,Venkitaraman AR
R960-25 was used in Western Blotting to show substrate discrimination and mitotic chromosome segregation to preserve genome integrity by human polo-like kinase PLK1 is mediated by a hydrophobic tryosine pocket in the polo-box domain.
Mon Nov 04 00:00:00 UTC 2019
Functional divergence of Plexin B structural motifs in distinct steps of Drosophila olfactory circuit assembly.
eLife
Guajardo R,Luginbuhl DJ,Han S,Luo L,Li J
R960-25 was used in Western Blotting to determine how Plexins employ their structural motifs in vivo to perform distinct roles.
Fri Jun 21 00:00:00 UTC 2019
Allele-specific RNA interference prevents neuropathy in Charcot-Marie-Tooth disease type 2D mouse models.
The Journal of clinical investigation
Morelli KH,Griffin LB,Pyne NK,Wallace LM,Fowler AM,Oprescu SN,Takase R,Wei N,Meyer-Schuman R,Mellacheruvu D,Kitzman JO,Kocen SG,Hines TJ,Spaulding EL,Lupski JR,Nesvizhskii A,Mancias P,Butler IJ,Yang XL,Hou YM,Antonellis A,Harper SQ,Burgess RW
R960-25 was used in Western Blot to show the efficacy of allele-specific RNAi as a potential therapy for Charcot-Marie-Tooth disease type 2D (CMT2D), caused by dominant mutations in glycyl-tRNA synthetase (GARS).
Mon Dec 02 00:00:00 UTC 2019
Prohibitin is a prognostic marker and therapeutic target to block chemotherapy resistance in Wilms' tumor.
JCI insight
Ortiz MV,Ahmed S,Burns M,Henssen AG,Hollmann TJ,MacArthur I,Gunasekera S,Gaewsky L,Bradwin G,Ryan J,Letai A,He Y,Naranjo A,Chi YY,LaQuaglia M,Heaton T,Cifani P,Dome JS,Gadd S,Perlman E,Mullen E,Steen H,Kentsis A
R960-25 was used in Western Blotting to identify urine tumor markers associated with Wilms' tumor relapse.
Thu Aug 08 00:00:00 UTC 2019
KIAA0317 regulates pulmonary inflammation through SOCS2 degradation.
JCI insight
Lear TB,McKelvey AC,Evankovich JW,Rajbhandari S,Coon TA,Dunn SR,Londino JD,McVerry BJ,Zhang Y,Valenzi E,Burton CL,Gordon R,Gingras S,Lockwood KC,Jurczak MJ,Lafyatis R,Shlomchik MJ,Liu Y,Chen BB
R960-25 was used in Western Blotting to investigate if the suppressors of cytokine signaling protein SOCS2 can be regulated by the action of the ubiquitin E3 ligase KIAA0317.
Thu Oct 03 00:00:00 UTC 2019
A Role for Drosophila Amyloid Precursor Protein in Retrograde Trafficking of L1-Type Cell Adhesion Molecule Neuroglian.
Frontiers in cellular neuroscience
Penserga T,Kudumala SR,Poulos R,Godenschwege TA
Thu Jul 18 00:00:00 UTC 2024
A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion.
Nature communications
Boone PG,Rochelle LK,Ginzel JD,Lubkov V,Roberts WL,Nicholls PJ,Bock C,Flowers ML,von Furstenberg RJ,Stripp BR,Agarwal P,Borowsky AD,Cardiff RD,Barak LS,Caron MG,Lyerly HK,Snyder JC
R960-25 was used in Immunocytochemistry to use a Cancer rainbow (Crainbow) modelling system for fluorescently barcoding somatic mutations and directly visualizing the clonal expansion and spread of oncogenes.
Mon Dec 02 00:00:00 UTC 2019
Partial proteasomal degradation of Lola triggers the male-to-female switch of a dimorphic courtship circuit.
Nature communications
Sato K,Ito H,Yokoyama A,Toba G,Yamamoto D
R960-25 was used in Immunoprecipitation to demonstrate that partial proteasomal degradation of Lola triggers the male-to-female switch of a dimorphic courtship circuit in Drosophila.
Fri Jan 11 00:00:00 UTC 2019
Determining the molecular drivers of species-specific interferon-stimulated gene product 15 interactions with nairovirus ovarian tumor domain proteases.
PloS one
Dzimianski JV,Scholte FEM,Williams IL,Langley C,Freitas BT,Spengler JR,Bergeron É,Pegan SD
R960CUS was used in Western Blotting to provide a foundation to further develop research methods to trace nairovirus-host relationships and delineate the full impact of ISG15 diversity on nairovirus infection.
Wed Apr 15 00:00:00 UTC 2020
Transcriptional dysregulation by a nucleus-localized aminoacyl-tRNA synthetase associated with Charcot-Marie-Tooth neuropathy.
Nature communications
Bervoets S,Wei N,Erfurth ML,Yusein-Myashkova S,Ermanoska B,Mateiu L,Asselbergh B,Blocquel D,Kakad P,Penserga T,Thomas FP,Guergueltcheva V,Tournev I,Godenschwege T,Jordanova A,Yang XL
R960CUS was used in Immunoprecipitation to highlight that tyrosyl-tRNA synthetase contributes to transcriptional regulation in neurons associated with Charcot-Marie-Tooth disease.
Wed Nov 06 00:00:00 UTC 2019
Apolipoprotein L9 interacts with LC3/GABARAP and is a microtubule-associated protein with a widespread subcellular distribution.
Biology open
Thekkinghat AA,Yadav KK,Rangarajan PN
R960-25 was used in Immunocytochemistry to show that ApoL9 interacts with the proteins of the LC3 and GABARAP subfamilies, which are key players in macroautophagy.
Wed Sep 25 00:00:00 UTC 2019
Apolipoprotein L9 interacts with LC3/GABARAP and is a microtubule-associated protein with a widespread subcellular distribution.
Biology open
Thekkinghat AA,Yadav KK,Rangarajan PN
R960-25 was used in Immunocytochemistry to show that ApoL9 interacts with the proteins of the LC3 and GABARAP subfamilies, which are key players in macroautophagy.
Wed Sep 25 00:00:00 UTC 2019
Severe Acute Respiratory Syndrome Coronavirus Viroporin 3a Activates the NLRP3 Inflammasome.
Frontiers in microbiology
Chen IY,Moriyama M,Chang MF,Ichinohe T
R960-25 was used in Western Blotting to study the mechanism by which severe acute respiratory syndrome coronavirus activates the Nod-like receptor family, pyrin domain-containing 3 inflammasome.
Thu Oct 01 00:00:00 UTC 2020
The conserved aspartate ring of MCU mediates MICU1 binding and regulation in the mitochondrial calcium uniporter complex.
eLife
Phillips CB,Tsai CW,Tsai MF
R960-25 was used in Western Blotting to determine the mechanism of Ca2+-dependent activation of the mitochondrial calcium uniporter.
Tue Jan 15 00:00:00 UTC 2019
Mitochondrial GTP Links Nutrient Sensing to β Cell Health, Mitochondrial Morphology, and Insulin Secretion Independent of OxPhos.
Cell reports
Jesinkey SR,Madiraju AK,Alves TC,Yarborough OH,Cardone RL,Zhao X,Parsaei Y,Nasiri AR,Butrico G,Liu X,Molina AJ,Rountree AM,Neal AS,Wolf DM,Sterpka J,Philbrick WM,Sweet IR,Shirihai OH,Kibbey RG
R960-25 was used in Western Blotting to highlight the importance of the mtGTP signal in nutrient sensing, insulin secretion, mitochondrial maintenance, and β cell health.
Tue Jul 16 00:00:00 UTC 2019
SAMHD1 Modulates Early Steps during Human Cytomegalovirus Infection by Limiting NF-κB Activation.
Cell reports
Kim ET,Roche KL,Kulej K,Spruce LA,Seeholzer SH,Coen DM,Diaz-Griffero F,Murphy EA,Weitzman MD
R960-25 was used in Western Blotting to explore the influence of SAMHD1 on human cytomegalovirus.
Tue Jul 09 00:00:00 UTC 2019
Distinct role of Sirtuin 1 (SIRT1) and Sirtuin 2 (SIRT2) in inhibiting cargo-loading and release of extracellular vesicles.
Scientific reports
Lee BR,Sanstrum BJ,Liu Y,Kwon SH
R960-25 was used in Western Blotting to suggest Sirtuin 2 regulates cargo loading to multivesicular bodies and multivesicular bodies to extracellular vesicles flux through a mechanism distrinct from that of Sirtuin 1.
Fri Dec 27 00:00:00 UTC 2019
The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop.
eLife
Abdusselamoglu MD,Eroglu E,Burkard TR,Knoblich JA
R960-25 was used in Immunohistochemistry to develop a method to isolate transcriptomes of intermediate neural progenitors in their distinct temporal states to identify a complete set of temporal patterning factors.
Mon Jul 22 00:00:00 UTC 2019
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
Nature communications
Fragoza R,Das J,Wierbowski SD,Liang J,Tran TN,Liang S,Beltran JF,Rivera-Erick CA,Ye K,Wang TY,Yao L,Mort M,Stenson PD,Cooper DN,Wei X,Keinan A,Schimenti JC,Clark AG,Yu H
R960-25 was used in Western Blotting to establish that 10.5% of missense variants carried per individual are disruptive indicating that each individual's genetic makeup may be more complex than expected.
Thu Sep 12 00:00:00 UTC 2019
Direct Gαq Gating Is the Sole Mechanism for TRPM8 Inhibition Caused by Bradykinin Receptor Activation.
Cell reports
Zhang X
R960-25 was used in Western Blot to demonstrate that direct Gαq gating is essential for both the basal cold sensitivity of TRPM8 and TRPM8 inhibition elicited by bradykinin in sensory neurons.
Tue Jun 18 00:00:00 UTC 2019
A pan-cancer analysis of synonymous mutations.
Nature communications
Sharma Y,Miladi M,Dukare S,Boulay K,Caudron-Herger M,Groß M,Backofen R,Diederichs S
R960-25 was used in Western Blotting to compile 659194 synonymous mutations found in human cancer and characterize their properties.
Wed Jun 12 00:00:00 UTC 2019
TRIM5α Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation.
Cell reports
Chiramel AI,Meyerson NR,McNally KL,Broeckel RM,Montoya VR,Méndez-Solís O,Robertson SJ,Sturdevant GL,Lubick KJ,Nair V,Youseff BH,Ireland RM,Bosio CM,Kim K,Luban J,Hirsch VM,Taylor RT,Bouamr F,Sawyer SL,Best SM
R960-25 was used in Western Blotting to show that both human and rhesus macaque TRIM5α suppress replication of specific flaviviruses.
Tue Jun 11 00:00:00 UTC 2019
RNase H1 promotes replication fork progression through oppositely transcribed regions of Drosophila mitochondrial DNA.
The Journal of biological chemistry
González de Cózar JM,Gerards M,Teeri E,George J,Dufour E,Jacobs HT,Jõers P
R960-25 was used in Western Blotting to study the effects of RNase H1 knockdown on mitochondrial RNA and DNA syntheses.
Fri Mar 22 00:00:00 UTC 2019
ASPP proteins discriminate between PP1 catalytic subunits through their SH3 domain and the PP1 C-tail.
Nature communications
Bertran MT,Mouilleron S,Zhou Y,Bajaj R,Uliana F,Kumar GS,van Drogen A,Lee R,Banerjee JJ,Hauri S,O'Reilly N,Gstaiger M,Page R,Peti W,Tapon N
R960-25 was used in Western Blotting to demonstrate that Drosophila ASPP is part of a multiprotein PP1 complex and that PP1 association is necessary for several in vivo functions of Drosophila ASPP.
Fri Feb 15 00:00:00 UTC 2019
Non-canonical ubiquitination of the cholesterol-regulated degron of squalene monooxygenase.
The Journal of biological chemistry
Chua NK,Hart-Smith G,Brown AJ
R960-25 was used in Western Blotting to reveal the degron architecture of Squalene Monooxygenase (SM) N100, the role of non-canonical ubiquitination sites and how SM is degraded in response to cholesterol.
Fri May 17 00:00:00 UTC 2019
A DNA telomerase vaccine for canine cancer immunotherapy.
Oncotarget
Thalmensi J,Pliquet E,Liard C,Chamel G,Kreuz C,Bestetti T,Escande M,Kostrzak A,Pailhes-Jimenez AS,Bourges E,Julithe M,Bourre L,Keravel O,Clayette P,Huet T,Wain-Hobson S,Langlade-Demoyen P
R960-25 was used in Immunocytochemistry to highlight the potential of pDUV5 as a cancer vaccine and supports its evaluation for the treatment of spontaneous canine tumors.
Tue May 21 00:00:00 UTC 2019
RIPK3 Activation Leads to Cytokine Synthesis that Continues after Loss of Cell Membrane Integrity.
Cell reports
Orozco SL,Daniels BP,Yatim N,Messmer MN,Quarato G,Chen-Harris H,Cullen SP,Snyder AG,Ralli-Jain P,Frase S,Tait SWG,Green DR,Albert ML,Oberst A
R960-25 was used in Western Blotting to investigate if cytokine production continues within necroptotic cells even after they have lost cell membrane integrity and irreversibly committed to death.
Tue Aug 27 00:00:00 UTC 2019
Arkadia/RNF111 is a SUMO-targeted ubiquitin ligase with preference for substrates marked with SUMO1-capped SUMO2/3 chain.
Nature communications
Sriramachandran AM,Meyer-Teschendorf K,Pabst S,Ulrich HD,Gehring NH,Hofmann K,Praefcke GJK,Dohmen RJ
R960-25 was used in Western Blotting to investigate if Arkadia specifically selects substrates carrying SUMO1-capped SUMO2/3 hybrid conjugates and if it targets them for proteasomal degradation.
Thu Aug 15 00:00:00 UTC 2019
Myxoma virus M013 protein antagonizes NF-κB and inflammasome pathways via distinct structural motifs.
The Journal of biological chemistry
Garg RR,Jackson CB,Rahman MM,Khan AR,Lewin AS,McFadden G
R960-25 was used in Western Blotting to characterise the mechanism of action of M013 interactions with cellar protein components of ASC-1 and NF-kB1 pathways.
Fri May 24 00:00:00 UTC 2019
Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida.
Scientific reports
Rodrigues IS,Pereira LMG,Lisboa J,Pereira C,Oliveira P,Dos Santos NMS,do Vale A
R960-25 was used in Western Blotting to explore the mechanisms through which the metalloprotease toxin AIP56 from Photobacterium damselae subsp. piscicida. causes intoxication, with specific focus on its interaction with host Hsp90 and cyclophilins.
Fri Jun 21 00:00:00 UTC 2019
Efficient system for upstream mRNA trans-splicing to generate covalent, head-to-tail, protein multimers.
Scientific reports
Mitsuhashi H,Homma S,Beermann ML,Ishimaru S,Takeda H,Yu BK,Liu K,Duraiswamy S,Boyce FM,Miller JB
R960-25 was used in Western Blotting to present a plasmid-based system in which upstream trans-splicing efficiently generates mRNAs that encode head-to-tail protein multimers.
Tue Feb 19 00:00:00 UTC 2019
Transsynaptic interactions between IgSF proteins DIP-α and Dpr10 are required for motor neuron targeting specificity.
eLife
Ashley J,Sorrentino V,Lobb-Rabe M,Nagarkar-Jaiswal S,Tan L,Xu S,Xiao Q,Zinn K,Carrillo RA
R960-25 was used in Immunohistochemistry-immunofluorescence to show that DIP-α is expressed by two identified motor neurons, while its binding partner Dpr10 is expressed by postsynaptic muscle targets.
Mon Feb 04 00:00:00 UTC 2019
Depletion of dAKAP1-protein kinase A signaling islands from the outer mitochondrial membrane alters breast cancer cell metabolism and motility.
The Journal of biological chemistry
Aggarwal S,Gabrovsek L,Langeberg LK,Golkowski M,Ong SE,Smith FD,Scott JD
R960CUS was used in Western Blot to propose that depletion of dAKAP1-PKA signaling islands" from the outer mitochondrial membrane augments progression toward metastatic breast cancer."
Fri Mar 01 00:00:00 UTC 2019
The Exchangeable Apolipoprotein Nplp2 Sustains Lipid Flow and Heat Acclimation in Drosophila.
Cell reports
Rommelaere S,Boquete JP,Piton J,Kondo S,Lemaitre B
R960-25 was used in Immunohistochemistry-immunofluorescence to study the effect of Nplp2 in thermal acclimation in Drosophila.
Tue Apr 16 00:00:00 UTC 2019
The Expression of the Chemokine CXCL14 Correlates with Several Aggressive Aspects of Glioblastoma and Promotes Key Properties of Glioblastoma Cells.
International journal of molecular sciences
Fazi B,Proserpio C,Galardi S,Annesi F,Cola M,Mangiola A,Michienzi A,Ciafrè SA
R960-25 was used in Western Blotting to point to CXCL14 as a protumorigenic chemokine in GBM.
Tue May 21 00:00:00 UTC 2019
A Multidimensional Characterization of E3 Ubiquitin Ligase and Substrate Interaction Network.
iScience
Chen D,Liu X,Xia T,Tekcham DS,Wang W,Chen H,Li T,Lu C,Ning Z,Liu X,Liu J,Qi H,He H,Piao HL
R960-25 was used in Immunoprecipitation to characterise and identify E3 substrate interactions.
Fri Jun 28 00:00:00 UTC 2019
Stem cell-derived cranial and spinal motor neurons reveal proteostatic differences between ALS resistant and sensitive motor neurons.
eLife
An D,Fujiki R,Iannitelli DE,Smerdon JW,Maity S,Rose MF,Gelber A,Wanaselja EK,Yagudayeva I,Lee JY,Vogel C,Wichterle H,Engle EC,Mazzoni EO
R960-25 was used in Immunocytochemistry to show an ESC-based platform has identified a superior capacity to maintain a healthy proteome as a possible mechanism to resist ALS-induced neurodegeneration.
Mon Jun 03 00:00:00 UTC 2019
Effects of codon optimization on coagulation factor IX translation and structure: Implications for protein and gene therapies.
Scientific reports
Alexaki A,Hettiarachchi GK,Athey JC,Katneni UK,Simhadri V,Hamasaki-Katagiri N,Nanavaty P,Lin B,Takeda K,Freedberg D,Monroe D,McGill JR,Peters R,Kames JM,Holcomb DD,Hunt RC,Sauna ZE,Gelinas A,Janjic N,DiCuccio M,Bar H,Komar AA,Kimchi-Sarfaty C
R960-25 was used in SDS-PAGE to investigate the impact of synonymous codons usage on protein expression and function.
Tue Oct 29 00:00:00 UTC 2019
Comprehensive Genetic Characterization of Mitochondrial Ca2+ Uniporter Components Reveals Their Different Physiological Requirements In Vivo.
Cell reports
Tufi R,Gleeson TP,von Stockum S,Hewitt VL,Lee JJ,Terriente-Felix A,Sanchez-Martinez A,Ziviani E,Whitworth AJ
R960-25 was used in Western Blotting to detail the genetic characteristics of all known uniporter components conserved in Drosophila.
Tue Apr 30 00:00:00 UTC 2019
Determining cellular CTCF and cohesin abundances to constrain 3D genome models.
eLife
Cattoglio C,Pustova I,Walther N,Ho JJ,Hantsche-Grininger M,Inouye CJ,Hossain MJ,Dailey GM,Ellenberg J,Darzacq X,Tjian R,Hansen AS
R960-25 was used in Western Blotting to establish tools for measuring cellular protein abundances to better understand 3D genome organisation quantitatively.
Mon Jun 17 00:00:00 UTC 2019
The transporters SLC35A1 and SLC30A1 play opposite roles in cell survival upon VSV virus infection.
Scientific reports
Moskovskich A,Goldmann U,Kartnig F,Lindinger S,Konecka J,Fiume G,Girardi E,Superti-Furga G
R960-25 was used in Western Blotting to characterise the role of host solute carriers in cell survival upon viral infection by performing unbiased genetic screens using a focused CRISPR knockout library.
Thu Jul 18 00:00:00 UTC 2019
Spatial regulation of GPR64/ADGRG2 signaling by β-arrestins and GPCR kinases.
Annals of the New York Academy of Sciences
Azimzadeh P,Talamantez-Lyburn SC,Chang KT,Inoue A,Balenga N
R960-25 was used in Western Blot to suggest that NTF not only shields the tethered agonist to prevent G protein signaling but also confers a conformation that inhibits the interaction with β-arrestins and the consequent endocytosis and sustained signaling from endosomes.
Fri Nov 01 00:00:00 UTC 2019
Stable Occupancy of the Crimean-Congo Hemorrhagic Fever Virus-Encoded Deubiquitinase Blocks Viral Infection.
mBio
Scholte FEM,Hua BL,Spengler JR,Dzimianski JV,Coleman-McCray JD,Welch SR,McMullan LK,Nichol ST,Pegan SD,Spiropoulou CF,Bergeron É
R960CUS was used in Western Blotting to demonstrate that artificially prolonged binding of the ovarian tumour (OTU) to a substrate inhibits virus infection, which provides novel insights into CCHFV OTU function during the viral replicative cycle and highlights the OTU as a potential antiviral target.
Tue Jul 23 00:00:00 UTC 2019
Srlp is crucial for the self-renewal and differentiation of germline stem cells via RpL6 signals in Drosophila testes.
Cell death & disease
Yu J,Yan Y,Luan X,Qiao C,Liu Y,Zhao D,Xie B,Zheng Q,Wang M,Chen W,Shen C,He Z,Hu X,Huang X,Li H,Shao Q,Chen X,Zheng B,Fang J
R960-25 was used in Immunohistochemistry to determine the underlying mechanistic links between Srlp and the stem cell niche.
Mon Apr 01 00:00:00 UTC 2019
ARID1A deficiency promotes mutability and potentiates therapeutic antitumor immunity unleashed by immune checkpoint blockade.
Nature medicine
Shen J,Ju Z,Zhao W,Wang L,Peng Y,Ge Z,Nagel ZD,Zou J,Wang C,Kapoor P,Ma X,Ma D,Liang J,Song S,Liu J,Samson LD,Ajani JA,Li GM,Liang H,Shen X,Mills GB,Peng G
R960-25 was used in Western Blotting to identify the molecular consequences of ARID1A deficiency that create therapeutic vulnerabilities in ARID1A-mutant tumours.
Tue May 01 00:00:00 UTC 2018
Widespread intronic polyadenylation inactivates tumour suppressor genes in leukaemia.
Nature
Lee SH,Singh I,Tisdale S,Abdel-Wahab O,Leslie CS,Mayr C
R960-25 was used in Immunoprecipitation to show the need to go beyond genomic analyses in cancer diagnostics, as mRNA events that are silent at the DNA level are widespread contributors to cancer pathogenesis through the inactivation of tumour-suppressor genes.
Sat Sep 01 00:00:00 UTC 2018
CDK12 regulates DNA repair genes by suppressing intronic polyadenylation.
Nature
Dubbury SJ,Boutz PL,Sharp PA
R960-25 was used in Western Blotting to study the mechanism by which CDK12 regulates homologous recombination genes.
Sat Dec 01 00:00:00 UTC 2018
Cyclic-Nucleotide- and HCN-Channel-Mediated Phototransduction in Intrinsically Photosensitive Retinal Ganglion Cells.
Cell
Jiang Z,Yue WWS,Chen L,Sheng Y,Yau KW
R960-25 was used in Immunohistochemistry to reveal a complex heterogeneity in phototransduction among ipRGCs and, more importantly, break a general dogma about segregation of the two phototransduction motifs, likely with strong evolutionary implications.
Thu Oct 18 00:00:00 UTC 2018
Proximity-CLIP provides a snapshot of protein-occupied RNA elements in subcellular compartments.
Nature methods
Benhalevy D,Anastasakis DG,Hafner M
R960-25 was used in Immunocytochemistry-immunoflourescence to observe that mRNAs localized to cell-cell interfaces often encoded regulatory proteins and contained protein-occupied CUG sequence elements in their 3' untranslated region.
Sat Dec 01 00:00:00 UTC 2018
An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders.
Nature genetics
Chen S,Fragoza R,Klei L,Liu Y,Wang J,Roeder K,Devlin B,Yu H
R960-25 was used in Western Blotting to demonstrate that our interactome perturbation approach offers a generalizable framework for identifying and prioritizing missense mutations that contribute to the risk of human disease.
Sun Jul 01 00:00:00 UTC 2018
CRISPR-Cas9 screens in human cells and primary neurons identify modifiers of C9ORF72 dipeptide-repeat-protein toxicity.
Nature genetics
Kramer NJ,Haney MS,Morgens DW,Jovičić A,Couthouis J,Li A,Ousey J,Ma R,Bieri G,Tsui CK,Shi Y,Hertz NT,Tessier-Lavigne M,Ichida JK,Bassik MC,Gitler AD
R960-25 was used in Immunocytochemistry-immunoflourescence to demonstrate the promise of CRISPR-Cas9 screens in defining mechanisms of neurodegenerative diseases.
Sun Apr 01 00:00:00 UTC 2018
A-to-I RNA Editing Contributes to Proteomic Diversity in Cancer.
Cancer cell
Peng X,Xu X,Wang Y,Hawke DH,Yu S,Han L,Zhou Z,Mojumdar K,Jeong KJ,Labrie M,Tsang YH,Zhang M,Lu Y,Hwu P,Scott KL,Liang H,Mills GB
R960-25 was used in Western Blotting to show cancerous protein diversity is promoted by adenosine (A) to inosine (I) RNA editing.
Mon May 14 00:00:00 UTC 2018
SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis.
Cancer cell
Fang L,Teng H,Wang Y,Liao G,Weng L,Li Y,Wang X,Jin J,Jiao C,Chen L,Peng X,Chen J,Yang Y,Fang H,Han D,Li C,Jin X,Zhang S,Liu Z,Liu M,Wei Q,Liao L,Ge X,Zhao B,Zhou D,Qin HL,Zhou J,Wang P
R960-25 was used in Western Blotting to study the mechanisms regulating YAP protein's nuclear export.
Mon Jul 09 00:00:00 UTC 2018
Allele-Specific Mechanisms of Activation of MEK1 Mutants Determine Their Properties.
Cancer discovery
Gao Y,Chang MT,McKay D,Na N,Zhou B,Yaeger R,Torres NM,Muniz K,Drosten M,Barbacid M,Caponigro G,Stuart D,Moebitz H,Solit DB,Abdel-Wahab O,Taylor BS,Yao Z,Rosen N
R960-25 was used in Western Blotting to develop a novel therapeutic strategy for tumours driven by RAF-independent MEK1 mutants.
Tue May 01 00:00:00 UTC 2018
Somatic Superenhancer Duplications and Hotspot Mutations Lead to Oncogenic Activation of the KLF5 Transcription Factor.
Cancer discovery
Zhang X,Choi PS,Francis JM,Gao GF,Campbell JD,Ramachandran A,Mitsuishi Y,Ha G,Shih J,Vazquez F,Tsherniak A,Taylor AM,Zhou J,Wu Z,Berger AC,Giannakis M,Hahn WC,Cherniack AD,Meyerson M
R960-25 was used in Chromatin immunoprecipitation to elucidate the three activating mechanisms that cause the pro-tumourigenic actions of Kruppel-like factor 5 gene.
Mon Jan 01 00:00:00 UTC 2018
Phospholipase PLA2G6, a Parkinsonism-Associated Gene, Affects Vps26 and Vps35, Retromer Function, and Ceramide Levels, Similar to α-Synuclein Gain.
Cell metabolism
Lin G,Lee PT,Chen K,Mao D,Tan KL,Zuo Z,Lin WW,Wang L,Bellen HJ
R960-25 was used in Western Blotting to show that loss of iPLA2-VIA, the fly homolog of PLA2G6, reduces lifespan, impairs synaptic transmission, and causes neurodegeneration.
Tue Oct 02 00:00:00 UTC 2018
Inflammation-Modulated Metabolic Reprogramming Is Required for DUOX-Dependent Gut Immunity in Drosophila.
Cell host & microbe
Lee KA,Cho KC,Kim B,Jang IH,Nam K,Kwon YE,Kim M,Hyeon DY,Hwang D,Seol JH,Lee WJ
R960-25 was used in Immunocytochemistry to reveal signalling cascades governing inflammation-induced metabolic reprogramming and provides insight into the pathophysiology of immune-metabolic interactions in the microbe-laden gut epithelia.
Wed Mar 14 00:00:00 UTC 2018
Spt6 Is Required for the Fidelity of Promoter Selection.
Molecular cell
Doris SM,Chuang J,Viktorovskaya O,Murawska M,Spatt D,Churchman LS,Winston F
R960-25 was used in Western Blotting to observe that fidelity of transcription initiation is controlled by Spt6 throughout the genome.
Thu Nov 15 00:00:00 UTC 2018
Deregulated Expression of Mammalian lncRNA through Loss of SPT6 Induces R-Loop Formation, Replication Stress, and Cellular Senescence.
Molecular cell
Nojima T,Tellier M,Foxwell J,Ribeiro de Almeida C,Tan-Wong SM,Dhir S,Dujardin G,Dhir A,Murphy S,Proudfoot NJ
R960-25 was used in Western Blotting to underline the importance of restricting lncRNA expression.
Thu Dec 20 00:00:00 UTC 2018
Targeting p53-dependent stem cell loss for intestinal chemoprotection.
Science translational medicine
Leibowitz BJ,Yang L,Wei L,Buchanan ME,Rachid M,Parise RA,Beumer JH,Eiseman JL,Schoen RE,Zhang L,Yu J
R960-25 was used in Western Blotting to demonstrate that p53-dependent PUMA induction mediates chemotherapy-induced intestinal injury in mice.
Wed Feb 07 00:00:00 UTC 2018
Methylation of hypoxia-inducible factor (HIF)-1α by G9a/GLP inhibits HIF-1 transcriptional activity and cell migration.
Nucleic acids research
Bao L,Chen Y,Lai HT,Wu SY,Wang JE,Hatanpaa KJ,Raisanen JM,Fontenot M,Lega B,Chiang CM,Semenza GL,Wang Y,Luo W
R960-25 was used in Immunoprecipitation to uncover a hypoxia-induced negative feedback mechanism that maintains high activity of hypoxia-inducible factor 1 and cell mobility in human glioblastoma.
Fri Jul 27 00:00:00 UTC 2018
Methylation of hypoxia-inducible factor (HIF)-1α by G9a/GLP inhibits HIF-1 transcriptional activity and cell migration.
Nucleic acids research
Bao L,Chen Y,Lai HT,Wu SY,Wang JE,Hatanpaa KJ,Raisanen JM,Fontenot M,Lega B,Chiang CM,Semenza GL,Wang Y,Luo W
R960-25 was used in Immunoprecipitation to uncover a hypoxia-induced negative feedback mechanism that maintains high activity of hypoxia-inducible factor 1 and cell mobility in human glioblastoma.
Fri Jul 27 00:00:00 UTC 2018
Enterovirus 71 antagonizes the inhibition of the host intrinsic antiviral factor A3G.
Nucleic acids research
Li Z,Ning S,Su X,Liu X,Wang H,Liu Y,Zheng W,Zheng B,Yu XF,Zhang W
R960-25 was used in Western Blotting to study the inhibition of enterovirus 71 virus replication by APOBEC3G.
Fri Nov 30 00:00:00 UTC 2018
The structure of human Nocturnin reveals a conserved ribonuclease domain that represses target transcript translation and abundance in cells.
Nucleic acids research
Abshire ET,Chasseur J,Bohn JA,Del Rizzo PA,Freddolino PL,Goldstrohm AC,Trievel RC
R960-25 was used in Western Blotting to study the mechanisms by which Nocturnin regulates high-fat diet induced weight gain, and bone formation.
Fri Jul 06 00:00:00 UTC 2018
Defective germline reprogramming rewires the spermatogonial transcriptome.
Nature structural & molecular biology
Vasiliauskaitė L,Berrens RV,Ivanova I,Carrieri C,Reik W,Enright AJ,O'Carroll D
R960-25 was used in Immunofluorescence on wholemounted tissues to demonstrate that defective reprogramming deregulates the spermatogonial transcriptome and may underlie spermatogonial dysfunction.
Tue May 01 00:00:00 UTC 2018
TLR5 decoy receptor as a novel anti-amyloid therapeutic for Alzheimer's disease.
The Journal of experimental medicine
Chakrabarty P,Li A,Ladd TB,Strickland MR,Koller EJ,Burgess JD,Funk CC,Cruz PE,Allen M,Yaroshenko M,Wang X,Younkin C,Reddy J,Lohrer B,Mehrke L,Moore BD,Liu X,Ceballos-Diaz C,Rosario AM,Medway C,Janus C,Li HD,Dickson DW,Giasson BI,Price ND,Younkin SG,Ertekin-Taner N,Golde TE
R960-25 was used in Western Blotting to explore whether a decoy receptor strategy using the ectodomain of select Toll-like receptors has therapeutic potential in Alzheimer's disease.
Mon Sep 03 00:00:00 UTC 2018
Loss of kallikrein-related peptidase 7 exacerbates amyloid pathology in Alzheimer's disease model mice.
EMBO molecular medicine
Kidana K,Tatebe T,Ito K,Hara N,Kakita A,Saito T,Takatori S,Ouchi Y,Ikeuchi T,Makino M,Saido TC,Akishita M,Iwatsubo T,Hori Y,Tomita T
R960-25 was used in Western Blotting to suggest that KLK7 aids in the degradation and clearance of deposited AΒ by astrocytes involved in the pathogenesis of AD.
Thu Mar 01 00:00:00 UTC 2018
Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms.
Developmental cell
Tan KL,Haelterman NA,Kwartler CS,Regalado ES,Lee PT,Nagarkar-Jaiswal S,Guo DC,Duraine L,Wangler MF,Bamshad MJ,Nickerson DA,Lin G,Milewicz DM,Bellen HJ
R960-25 was used in Western Blotting to study the role of ARIH1 in myonuclear organisation.
Mon Apr 23 00:00:00 UTC 2018
Crosstalk between lysine methylation and phosphorylation of ATG16L1 dictates the apoptosis of hypoxia/reoxygenation-induced cardiomyocytes.
Autophagy
Song H,Feng X,Zhang M,Jin X,Xu X,Wang L,Ding X,Luo Y,Lin F,Wu Q,Liang G,Yu T,Liu Q,Zhang Z
R960-25 was used in Western blotting following immunoprecipitation to define a novel modification of ATG16L1 and highlight the importance of an ATG16L1 phosphorylation-methylation switch in determining the fate of H/R-treated cardiomyocytes.
Thu Oct 03 00:00:00 UTC 2019
Crosstalk between lysine methylation and phosphorylation of ATG16L1 dictates the apoptosis of hypoxia/reoxygenation-induced cardiomyocytes.
Autophagy
Song H,Feng X,Zhang M,Jin X,Xu X,Wang L,Ding X,Luo Y,Lin F,Wu Q,Liang G,Yu T,Liu Q,Zhang Z
R960-25 was used in Western blotting following immunoprecipitation to define a novel modification of ATG16L1 and highlight the importance of an ATG16L1 phosphorylation-methylation switch in determining the fate of H/R-treated cardiomyocytes.
Thu Oct 03 00:00:00 UTC 2019
Glutamate-activated BK channel complexes formed with NMDA receptors.
Proceedings of the National Academy of Sciences of the United States of America
Zhang J,Guan X,Li Q,Meredith AL,Pan HL,Yan J
R960-25 was used in Proximity Ligation Assay to show that large-conductance calcium- and voltage-activated K+ channels form complexes with NMDA receptors in both rodent brains and a heterologous expression system.
Tue Sep 18 00:00:00 UTC 2018
PP2A negatively regulates the hypertrophic response by dephosphorylating HDAC2 S394 in the heart.
Experimental & molecular medicine
Yoon S,Kook T,Min HK,Kwon DH,Cho YK,Kim M,Shin S,Joung H,Jeong SH,Lee S,Kang G,Park Y,Kim YS,Ahn Y,McMullen JR,Gergs U,Neumann J,Kim KK,Kim J,Nam KI,Kim YK,Kook H,Eom GH
R960-25 was used in Immunoprecipitation to characterise the functional complex of histone deacetylase 2.
Thu Jul 26 00:00:00 UTC 2018
APP upregulation contributes to retinal ganglion cell degeneration via JNK3.
Cell death and differentiation
Liu C,Zhang CW,Zhou Y,Wong WQ,Lee LC,Ong WY,Yoon SO,Hong W,Fu XY,Soong TW,Koo EH,Stanton LW,Lim KL,Xiao ZC,Dawe GS
R960-25 was used in Chromatin immunoprecipitation to show that ONA induces APP expression and that gamma-secretase cleavage of APP releases AICD, which upregulates JNK3 leading to RGC death.
Thu Mar 01 00:00:00 UTC 2018
APP upregulation contributes to retinal ganglion cell degeneration via JNK3.
Cell death and differentiation
Liu C,Zhang CW,Zhou Y,Wong WQ,Lee LC,Ong WY,Yoon SO,Hong W,Fu XY,Soong TW,Koo EH,Stanton LW,Lim KL,Xiao ZC,Dawe GS
R960-25 was used in Chromatin immunoprecipitation to show that ONA induces APP expression and that gamma-secretase cleavage of APP releases AICD, which upregulates JNK3 leading to RGC death.
Thu Mar 01 00:00:00 UTC 2018
Interrogation of Mammalian Protein Complex Structure, Function, and Membership Using Genome-Scale Fitness Screens.
Cell systems
Pan J,Meyers RM,Michel BC,Mashtalir N,Sizemore AE,Wells JN,Cassel SH,Vazquez F,Weir BA,Hahn WC,Marsh JA,Tsherniak A,Kadoch C
R960-25 was used in Immunocytochemistry-immunoflourescence to demonstrate the utility of genetic perturbation screens to enhance our understanding of mammalian protein complexes in normal and disease states.
Wed May 23 00:00:00 UTC 2018
Intracellular attenuation of BMP signaling via CKIP-1/Smurf1 is essential during neural crest induction.
PLoS biology
Piacentino ML,Bronner ME
R960-25 was used in Immunohistochemistry-Immunofluorescence and Western blotting to propose a morphogen model in which intracellular attenuation of bone morphogenetic protein signalling sets the required intermediate levels to maintain neural crest induction.
Fri Jun 01 00:00:00 UTC 2018
Intracellular attenuation of BMP signaling via CKIP-1/Smurf1 is essential during neural crest induction.
PLoS biology
Piacentino ML,Bronner ME
R960-25 was used in Immunohistochemistry-Immunofluorescence and Western blotting to propose a morphogen model in which intracellular attenuation of bone morphogenetic protein signalling sets the required intermediate levels to maintain neural crest induction.
Fri Jun 01 00:00:00 UTC 2018
ATM directs DNA damage responses and proteostasis via genetically separable pathways.
Science signaling
Lee JH,Mand MR,Kao CH,Zhou Y,Ryu SW,Richards AL,Coon JJ,Paull TT
R960-25 was used in Western Blotting to study the relationship between the mechanism of ATM activation and its effects on cellular metabolism and DNA damage responses in human cells.
Tue Jan 09 00:00:00 UTC 2018
Inhibition of AIM2 inflammasome activation by a novel transcript isoform of IFI16.
EMBO reports
Wang PH,Ye ZW,Deng JJ,Siu KL,Gao WW,Chaudhary V,Cheng Y,Fung SY,Yuen KS,Ho TH,Chan CP,Zhang Y,Kok KH,Yang W,Chan CP,Jin DY
R960CUS was used in Western Blotting to demonstrate that cytoplasm-localized IFI16-β is functionally equivalent to mouse p202 that exerts an inhibitory effect on AIM2 inflammasome.
Mon Oct 01 00:00:00 UTC 2018
A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer.
Oncogene
Howley BV,Link LA,Grelet S,El-Sabban M,Howe PH
R960-25 was used in Western Blotting to study the importance of the modulation of endoplasmic reticulum-golgi trafficking in metastatic progression of breast cancer.
Thu Mar 01 00:00:00 UTC 2018
c-Src activity is differentially required by cancer cell motility modes.
Oncogene
Logue JS,Cartagena-Rivera AX,Chadwick RS
R960-25 was used in Immunocytochemistry-immunoflourescence to evaluate the role of Src activity in mediating cancer cell motility.
Sun Apr 01 00:00:00 UTC 2018
The two TRAPP complexes of metazoans have distinct roles and act on different Rab GTPases.
The Journal of cell biology
Riedel F,Galindo A,Muschalik N,Munro S
R960-25 was used in Immunohistochemistry-immunofluorescence to show that Drosophila melanogaster have two transport protein particle complexes similar to those in mammals and that both activate Rab1, whereas one, TRAPPII, also activates Rab11.
Mon Feb 05 00:00:00 UTC 2018
PROHIBITIN3 Forms Complexes with ISOCHORISMATE SYNTHASE1 to Regulate Stress-Induced Salicylic Acid Biosynthesis in Arabidopsis.
Plant physiology
Seguel A,Jelenska J,Herrera-Vásquez A,Marr SK,Joyce MB,Gagesch KR,Shakoor N,Jiang SC,Fonseca A,Wildermuth MC,Greenberg JT,Holuigue L
R960-25 was used in Western Blotting to study the mechanism by which PHB3-ICS1 regulates stress induced Salicylic acid in response to stress.
Thu Mar 01 00:00:00 UTC 2018
Inhibition of Japanese encephalitis virus infection by the host zinc-finger antiviral protein.
PLoS pathogens
Chiu HP,Chiu H,Yang CF,Lee YL,Chiu FL,Kuo HC,Lin RJ,Lin YL
R960-25 was used in Immunoprecipitation to investigate the antiviral potential of human CCCH-type zinc-finger antiviral protein against three flaviviruses: Japanese encephalitis virus, dengue virus, and Zika virus.
Sun Jul 01 00:00:00 UTC 2018
The interferon-stimulated gene product oligoadenylate synthetase-like protein enhances replication of Kaposi's sarcoma-associated herpesvirus (KSHV) and interacts with the KSHV ORF20 protein.
PLoS pathogens
Bussey KA,Lau U,Schumann S,Gallo A,Osbelt L,Stempel M,Arnold C,Wissing J,Gad HH,Hartmann R,Brune W,Jänsch L,Whitehouse A,Brinkmann MM
R960-25 was used in Immunoprecipitation to characterise the interaction of Kaposi's sarcoma-associated herpesvirus ORF20 protein with oligoadenylate synthetase-like protein, whereby it usurps the function of OASL to benefit KSHV infection.
Thu Mar 01 00:00:00 UTC 2018
Hey2 regulates the size of the cardiac progenitor pool during vertebrate heart development.
Development (Cambridge, England)
Gibb N,Lazic S,Yuan X,Deshwar AR,Leslie M,Wilson MD,Scott IC
R960-25 was used in Immunohistochemistry-immunofluorescence to determine the mechanism underlying the regulation of the cardiac progenitor pool during development of the vertebrate heart.
Wed Nov 21 00:00:00 UTC 2018
Drosophila Sidekick is required in developing photoreceptors to enable visual motion detection.
Development (Cambridge, England)
Astigarraga S,Douthit J,Tarnogorska D,Creamer MS,Mano O,Clark DA,Meinertzhagen IA,Treisman JE
R960-25 was used in Immunohistochemistry to study the conserved requirement for Sidekick proteins in establishing visual motion detection circuits, achieved through distinct cellular mechanisms in Drosophila and vertebrates.
Mon Feb 05 00:00:00 UTC 2018
14-3-3 Proteins Reduce Cell-to-Cell Transfer and Propagation of Pathogenic α-Synuclein.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Wang B,Underwood R,Kamath A,Britain C,McFerrin MB,McLean PJ,Volpicelli-Daley LA,Whitaker RH,Placzek WJ,Becker K,Ma J,Yacoubian TA
R960-25 was used in Immunoprecipitation to investigate the potential role of the 14-3-3 proteins in the regulation of α-synuclein spread using two models of α-synuclein spread.
Wed Sep 19 00:00:00 UTC 2018
Paramyxovirus V Proteins Interact with the RIG-I/TRIM25 Regulatory Complex and Inhibit RIG-I Signaling.
Journal of virology
Sánchez-Aparicio MT,Feinman LJ,García-Sastre A,Shaw ML
R960-25 was used in Western Blot to elucidate a novel mechanism for innate immune inhibition by paramyxovirus V proteins, distinct from other known V protein functions such as MDA5 and STAT1 antagonism.
Thu Mar 15 00:00:00 UTC 2018
Varicella-Zoster Virus ORF9p Binding to Cellular Adaptor Protein Complex 1 Is Important for Viral Infectivity.
Journal of virology
Lebrun M,Lambert J,Riva L,Thelen N,Rambout X,Blondeau C,Thiry M,Snoeck R,Twizere JC,Dequiedt F,Andrei G,Sadzot-Delvaux C
R960-25 was used in Immunocytochemistry-immunoflourescence to identify cellular proteins interacting with ORF9p that might be important for viral infectivity.
Wed Aug 01 00:00:00 UTC 2018
The Human Papillomavirus E6 Oncoprotein Targets USP15 and TRIM25 To Suppress RIG-I-Mediated Innate Immune Signaling.
Journal of virology
Chiang C,Pauli EK,Biryukov J,Feister KF,Meng M,White EA,Münger K,Howley PM,Meyers C,Gack MU
R960-25 was used in Western Blotting to study the human papillomavirus 16 E6 oncoprotein mediated antagonisation of activation of cytoplasmic Retinoic acid-inducible gene-I.
Thu Mar 15 00:00:00 UTC 2018
H5N1 Influenza A Virus PB1-F2 Relieves HAX-1-Mediated Restriction of Avian Virus Polymerase PA in Human Lung Cells.
Journal of virology
Mazel-Sanchez B,Boal-Carvalho I,Silva F,Dijkman R,Schmolke M
R960-25 was used in Western Blotting to demonstrate that the PA of a mammal-adapted H1N1 influenza A viruses (IAV) is resistant to HCLS1-associated protein X1 imposed restriction, while the PA of an avian-origin H5N1 IAV remains sensitive.
Fri Jun 01 00:00:00 UTC 2018
A Survey of DDX21 Activity During Rev/RRE Complex Formation.
Journal of molecular biology
Hammond JA,Zhou L,Lamichhane R,Chu HY,Millar DP,Gerace L,Williamson JR
R960-25 was used in Western Blotting to examine DDX21 for protein and RNA binding and subsequent enzymatic activities in the context of the Rev/RRE pathway.
Fri Feb 16 00:00:00 UTC 2018
Ceramide Nanoliposomes as a MLKL-Dependent, Necroptosis-Inducing, Chemotherapeutic Reagent in Ovarian Cancer.
Molecular cancer therapeutics
Zhang X,Kitatani K,Toyoshima M,Ishibashi M,Usui T,Minato J,Egiz M,Shigeta S,Fox T,Deering T,Kester M,Yaegashi N
R960-25 was used in Western Blotting to investigate the therapeutic efficacy and signalling mechanisms of ceramide nanoliposomes in refractory ovarian cancer.
Mon Jan 01 00:00:00 UTC 2018
Functional analysis of corin protein domains required for PCSK6-mediated activation.
The international journal of biochemistry & cell biology
Chen S,Wang H,Li H,Zhang Y,Wu Q
R960-25 was used in Western Blotting to assess the molecular and cellular mechanisms underlying proprotein convertase subtilisin/kexin-6-mediated corin activation that is critical for cardiovascular homeostasis.
Mon Jan 01 00:00:00 UTC 2018
Mutations in the U11/U12-65K protein associated with isolated growth hormone deficiency lead to structural destabilization and impaired binding of U12 snRNA.
RNA (New York, N.Y.)
Norppa AJ,Kauppala TM,Heikkinen HA,Verma B,Iwaï H,Frilander MJ
R960-25 was used in Immunoprecipitation to characterise the biochemical effects on the 65K protein, of the defects previously identified in U12-type intron recognition in isolated growth hormone deficiency patient cells.
Thu Mar 01 00:00:00 UTC 2018
Characterization of a novel RP2-OSTF1 interaction and its implication for actin remodelling.
Journal of cell science
Lyraki R,Lokaj M,Soares DC,Little A,Vermeren M,Marsh JA,Wittinghofer A,Hurd T
R960-25 was used in Immunocytochemistry-immunoflourescence and Western blot to elucidate a novel interaction between retinitis pigmentosa 2 and osteoclast-stimulating factor 1, implicated in actin remodelling.
Tue Feb 20 00:00:00 UTC 2018
Characterization of a novel RP2-OSTF1 interaction and its implication for actin remodelling.
Journal of cell science
Lyraki R,Lokaj M,Soares DC,Little A,Vermeren M,Marsh JA,Wittinghofer A,Hurd T
R960-25 was used in Immunocytochemistry-immunoflourescence and Western blot to elucidate a novel interaction between retinitis pigmentosa 2 and osteoclast-stimulating factor 1, implicated in actin remodelling.
Tue Feb 20 00:00:00 UTC 2018
Ciliogenesis and cell cycle alterations contribute to KIF2A-related malformations of cortical development.
Human molecular genetics
Broix L,Asselin L,Silva CG,Ivanova EL,Tilly P,Gilet JG,Lebrun N,Jagline H,Muraca G,Saillour Y,Drouot N,Reilly ML,Francis F,Benmerah A,Bahi-Buisson N,Belvindrah R,Nguyen L,Godin JD,Chelly J,Hinckelmann MV
R960-25 was used in Immunohistochemistry to examine how ciliogenesis and cell cycle alterations contribute to KIF2A-related malformations of cortical development.
Mon Jan 15 00:00:00 UTC 2018
Regulation of ciliary function by fibroblast growth factor signaling identifies FGFR3-related disorders achondroplasia and thanatophoric dysplasia as ciliopathies.
Human molecular genetics
Kunova Bosakova M,Varecha M,Hampl M,Duran I,Nita A,Buchtova M,Dosedelova H,Machat R,Xie Y,Ni Z,Martin JH,Chen L,Jansen G,Krakow D,Krejci P
R960-25 was used in Western Blotting to integrate primary cilia into canonical FGF signal transduction and uncover a FGF-cilia pathway that needs consideration when elucidating the mechanisms of physiological and pathological FGFR function, or in the development of FGFR therapeutics.
Thu Mar 15 00:00:00 UTC 2018
Variable signaling activity by FOP ACVR1 mutations.
Bone
Haupt J,Xu M,Shore EM
R960-25 was used in Western Blotting to demonstrate that fibrodysplasia ossificans progressiva mutations within the ACVR1 receptor kinase domain are more sensitive to low levels of BMP than mutations in the ACVR1 GS domain.
Sun Apr 01 00:00:00 UTC 2018
N-linked glycans are required on epithelial Na+ channel subunits for maturation and surface expression.
American journal of physiology. Renal physiology
Kashlan OB,Kinlough CL,Myerburg MM,Shi S,Chen J,Blobner BM,Buck TM,Brodsky JL,Hughey RP,Kleyman TR
R960-25 was used in Western Blotting to examine the effect of preventing the N-glycosylation of specific epithelial Na+ channel subunits on channel function, expression, maturation, and folding.
Thu Mar 01 00:00:00 UTC 2018
Bdp1 interacts with SNAPc bound to a U6, but not U1, snRNA gene promoter element to establish a stable protein-DNA complex.
FEBS letters
Verma N,Hurlburt AM,Wolfe A,Kim MK,Kang YS,Kang JJ,Stumph WE
R960-25 was used in Western Blotting to show that, in Drosophila melanogaster, DmSNAPc directly recruits Bdp1 to the U6 promoter, and we identify an 87-residue region of Bdp1 involved in this interaction.
Sun Jul 01 00:00:00 UTC 2018
Asterless is a Polo-like kinase 4 substrate that both activates and inhibits kinase activity depending on its phosphorylation state.
Molecular biology of the cell
Boese CJ,Nye J,Buster DW,McLamarrah TA,Byrnes AE,Slep KC,Rusan NM,Rogers GC
R960-25 was used in Western Blotting to study how the N-terminus of Asl binds Polo-like kinase 4 (Plk4) and promotes Plk4 self-destruction.
Thu Nov 15 00:00:00 UTC 2018
Insertion position as well as the inserted TRS and gene sequences differentially affect the retention of foreign gene expression by simian hemorrhagic fever virus (SHFV).
Virology
Di H,Morantz EK,Sadhwani H,Madden JC,Brinton MA
R960-25 was used in Western Blotting to show that although regulation of SHFV subgenomic RNA synthesis is complex, the ORF4'/ORF2b site, which is located between the two sets of minor structural proteins, is able to tolerate foreign gene insertion.
Sat Dec 01 00:00:00 UTC 2018
Functional domains of the FSHD-associated DUX4 protein.
Biology open
Mitsuhashi H,Ishimaru S,Homma S,Yu B,Honma Y,Beermann ML,Miller JB
R960-25 was used in Western Blotting and ICC/IF to examine the functional domains of the facioscapulohumeral muscular dystrophy-associated DUX4 protein.
Thu Apr 26 00:00:00 UTC 2018
Functional domains of the FSHD-associated DUX4 protein.
Biology open
Mitsuhashi H,Ishimaru S,Homma S,Yu B,Honma Y,Beermann ML,Miller JB
R960-25 was used in Western Blotting and ICC/IF to examine the functional domains of the facioscapulohumeral muscular dystrophy-associated DUX4 protein.
Thu Apr 26 00:00:00 UTC 2018
Nuclear Lamin is required for Winged Eye-mediated transdetermination of Drosophila imaginal disc.
Genes to cells : devoted to molecular & cellular mechanisms
Masuko K,Furuhashi H,Komaba K,Numao E,Nakajima R,Fuse N,Kurata S
R960-25 was used in Western Blotting to study the molecular mechanism of Winged Eye - mediated transdetermination in Drosophila.
Mon Jul 02 00:00:00 UTC 2018
Purification of kinetochores from the budding yeast Saccharomyces cerevisiae.
Methods in cell biology
Gupta A,Evans RK,Koch LB,Littleton AJ,Biggins S
R960-25 was used in Immunoprecipitation to develop a technique to purify kinetochores from budding yeast that can be used to analyze kinetochore functions and composition.
Tue Dec 11 00:00:00 UTC 2018
Triglycerides Promote Lipid Homeostasis during Hypoxic Stress by Balancing Fatty Acid Saturation.
Cell reports
Ackerman D,Tumanov S,Qiu B,Michalopoulou E,Spata M,Azzam A,Xie H,Simon MC,Kamphorst JJ
R960-25 was used in Western Blot to study the mechanism by which triglycerides promote lipid homeostasis.
Tue Sep 04 00:00:00 UTC 2018
A compartmentalized signaling network mediates crossover control in meiosis.
eLife
Zhang L,Köhler S,Rillo-Bohn R,Dernburg AF
R960-25 was used in Western Blotting to investigate the functions of the proteins ZHP-1, ZHP-2, and ZHP-4.
Fri Mar 09 00:00:00 UTC 2018
A compartmentalized signaling network mediates crossover control in meiosis.
eLife
Zhang L,Köhler S,Rillo-Bohn R,Dernburg AF
R960-25 was used in Western Blotting to investigate the functions of the proteins ZHP-1, ZHP-2, and ZHP-4.
Fri Mar 09 00:00:00 UTC 2018
A Primate-Specific Isoform of PLEKHG6 Regulates Neurogenesis and Neuronal Migration.
Cell reports
O'Neill AC,Kyrousi C,Klaus J,Leventer RJ,Kirk EP,Fry A,Pilz DT,Morgan T,Jenkins ZA,Drukker M,Berkovic SF,Scheffer IE,Guerrini R,Markie DM,Götz M,Cappello S,Robertson SP
R960-25 was used in Western Blotting to determine the role of PLEKHG6 in primate brain development.
Tue Dec 04 00:00:00 UTC 2018
APOE ε2 is associated with increased tau pathology in primary tauopathy.
Nature communications
Zhao N,Liu CC,Van Ingelgom AJ,Linares C,Kurti A,Knight JA,Heckman MG,Diehl NN,Shinohara M,Martens YA,Attrebi ON,Petrucelli L,Fryer JD,Wszolek ZK,Graff-Radford NR,Caselli RJ,Sanchez-Contreras MY,Rademakers R,Murray ME,Koga S,Dickson DW,Ross OA,Bu G
R960-25 was used in Immunohistochemistry to evaluate the association of Apolipoprotein E genotype with the presence and severity of tau pathology.
Mon Oct 22 00:00:00 UTC 2018
Biochemical Analysis of Dimethyl Suberimidate-crosslinked Yeast Nucleosomes.
Bio-protocol
Ichiakwa Y,Kaufman PD
R960-25 was used in Western Blotting to provide a detailed protocol for the use of dimethyl suberimidate to analyse yeast nucleosomes.
Tue Mar 20 00:00:00 UTC 2018
The role of glycosyltransferase enzyme GCNT3 in colon and ovarian cancer prognosis and chemoresistance.
Scientific reports
Fernández LP,Sánchez-Martínez R,Vargas T,Herranz J,Martín-Hernández R,Mendiola M,Hardisson D,Reglero G,Feliu J,Redondo A,Ramírez de Molina A
R960-25 was used in Western Blotting to study the molecular basis of the role of GCNT3 in tumourigenesis and drug resistance, and its potential role as a biomarker in epithelial ovarian cancer.
Thu May 31 00:00:00 UTC 2018
The sulfite oxidase Shopper controls neuronal activity by regulating glutamate homeostasis in Drosophila ensheathing glia.
Nature communications
Otto N,Marelja Z,Schoofs A,Kranenburg H,Bittern J,Yildirim K,Berh D,Bethke M,Thomas S,Rode S,Risse B,Jiang X,Pankratz M,Leimkühler S,Klämbt C
R960-25 was used in Immunohistochemistry to elucidate the involvement of glial cells in the modulation of neuronal activity.
Wed Aug 29 00:00:00 UTC 2018
Stepwise wiring of the Drosophila olfactory map requires specific Plexin B levels.
eLife
Li J,Guajardo R,Xu C,Wu B,Li H,Li T,Luginbuhl DJ,Xie X,Luo L
R960-25 was used in Immunocytochemistry-immunoflourescence to examine the cell-type specific distribution of endogenous levels of Plexin B in the developing antennal lobe in Drosophila.
Thu Aug 23 00:00:00 UTC 2018
SIRT1 mediates obesity- and nutrient-dependent perturbation of pubertal timing by epigenetically controlling Kiss1 expression.
Nature communications
Vazquez MJ,Toro CA,Castellano JM,Ruiz-Pino F,Roa J,Beiroa D,Heras V,Velasco I,Dieguez C,Pinilla L,Gaytan F,Nogueiras R,Bosch MA,Rønnekleiv OK,Lomniczi A,Ojeda SR,Tena-Sempere M
R960-25 was used in Immunoprecipitation to identify Sirtuin 1, a fuel-sensing deacetylase, as a molecule that restrains female puberty via epigenetic repression of the puberty-activating gene, Kiss1.
Wed Oct 10 00:00:00 UTC 2018
Essential Role of the a3 Isoform of V-ATPase in Secretory Lysosome Trafficking via Rab7 Recruitment.
Scientific reports
Matsumoto N,Sekiya M,Tohyama K,Ishiyama-Matsuura E,Sun-Wada GH,Wada Y,Futai M,Nakanishi-Matsui M
R960-25 was used in Western Blotting to reveal a novel role for the proton pump Vacuolar-type ATPase in secretory lysosome trafficking and an unexpected mechanistic link with Rab GTPases.
Mon Apr 30 00:00:00 UTC 2018
Structure and evolution of the Fam20 kinases.
Nature communications
Zhang H,Zhu Q,Cui J,Wang Y,Chen MJ,Guo X,Tagliabracci VS,Dixon JE,Xiao J
R960-25 was used in Western Blotting to study the structural, biochemical and evolutionary nature of the function of the Fam20 kinases.
Fri Mar 23 00:00:00 UTC 2018
Cross-kingdom auxiliary subunit modulation of a voltage-gated sodium channel.
The Journal of biological chemistry
Molinarolo S,Lee S,Leisle L,Lueck JD,Granata D,Carnevale V,Ahern CA
R960-25 was used in Western Blotting to report that the eukaryotic voltage-gated sodium ion-selective channel beta subunit isoform interacts with the surface expression and voltage-dependent gating properties of the bacterial NaV in Xenopus oocytes.
Fri Apr 06 00:00:00 UTC 2018
ASF1 is required to load histones on the HIRA complex in preparation of paternal chromatin assembly at fertilization.
Epigenetics & chromatin
Horard B,Sapey-Triomphe L,Bonnefoy E,Loppin B
R960-25 was used in Western Blotting and Immunohistochemistry-Immunofluorescence to show that ASF1 is critically required to load H3.3-H4 dimers on the HIRA complex prior to histone deposition on paternal DNA.
Fri May 11 00:00:00 UTC 2018
ASF1 is required to load histones on the HIRA complex in preparation of paternal chromatin assembly at fertilization.
Epigenetics & chromatin
Horard B,Sapey-Triomphe L,Bonnefoy E,Loppin B
R960-25 was used in Western Blotting and Immunohistochemistry-Immunofluorescence to show that ASF1 is critically required to load H3.3-H4 dimers on the HIRA complex prior to histone deposition on paternal DNA.
Fri May 11 00:00:00 UTC 2018
A novel NAA10 variant with impaired acetyltransferase activity causes developmental delay, intellectual disability, and hypertrophic cardiomyopathy.
European journal of human genetics : EJHG
Støve SI,Blenski M,Stray-Pedersen A,Wierenga KJ,Jhangiani SN,Akdemir ZC,Crawford D,McTiernan N,Myklebust LM,Purcarin G,McNall-Knapp R,Wadley A,Belmont JW,Kim JJ,Lupski JR,Arnesen T
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot to investigate NAA10-NAA15 complex in genetic disorders.
Sat Sep 01 00:00:00 UTC 2018
Axin phosphorylation in both Wnt-off and Wnt-on states requires the tumor suppressor APC.
PLoS genetics
Tacchelly-Benites O,Wang Z,Yang E,Benchabane H,Tian A,Randall MP,Ahmed Y
R960-25 was used in Western Blotting to identify the requirement for tumor suppressor APC in axin regulation through phosphorylation of Wnt-on and off conditions.
Thu Feb 01 00:00:00 UTC 2018
Axin phosphorylation in both Wnt-off and Wnt-on states requires the tumor suppressor APC.
PLoS genetics
Tacchelly-Benites O,Wang Z,Yang E,Benchabane H,Tian A,Randall MP,Ahmed Y
R960-25 was used in Western Blotting to identify the requirement for tumor suppressor APC in axin regulation through phosphorylation of Wnt-on and off conditions.
Thu Feb 01 00:00:00 UTC 2018
Activation of EphB2 Forward Signaling Enhances Memory Consolidation.
Cell reports
Alapin JM,Dines M,Vassiliev M,Tamir T,Ram A,Locke C,Yu J,Lamprecht R
R960-25 was used in Western Blotting to explore the roles of EphB2 in memory formation and enhancement.
Tue May 15 00:00:00 UTC 2018
The binding of Chp2's chromodomain to methylated H3K9 is essential for Chp2's role in heterochromatin assembly in fission yeast.
PloS one
Maksimov V,Oya E,Tanaka M,Kawaguchi T,Hachisuka A,Ekwall K,Bjerling P,Nakayama JI
R960-25 was used in Western Blotting to demonstrate that Chp2's lysine 9-methylated histone H3 (H3K9me) binding is critical for SHREC function and that the two SHREC complex modules are interdependent.
Thu Jan 17 00:00:00 UTC 2019
MICU1 Confers Protection from MCU-Dependent Manganese Toxicity.
Cell reports
Wettmarshausen J,Goh V,Huang KT,Arduino DM,Tripathi U,Leimpek A,Cheng Y,Pittis AA,Gabaldón T,Mokranjac D,Hajnóczky G,Perocchi F
R960-25 was used in Western Blot to dissect the interdependence between the pore-forming subunit MCU and the calcium-sensing regulator MICU1.
Tue Nov 06 00:00:00 UTC 2018
Investigating the functionality of a ribosome-binding mutant of NAA15 using Saccharomyces cerevisiae.
BMC research notes
Varland S,Arnesen T
R960-25 was used in Western Blotting to investigate the in vivo functionality of a Schizosaccharomyces pombe NAA15 mutant that is known to prevent N-terminal acetyltransferase complex A (NatA) from associating with ribosomes, but retains NatA-specific activity.
Fri Jun 22 00:00:00 UTC 2018
PARP-1-dependent RND1 transcription induced by topoisomerase I cleavage complexes confers cellular resistance to camptothecin.
Cell death & disease
Mouly L,Mamouni K,Gence R,Cristini A,Cherier J,Castinel A,Legrand M,Favre G,Sordet O,Monferran S
R960-25 was used in Western Blotting to search for potential early DNA damage-inducible RHO GTPase genes.
Wed Sep 12 00:00:00 UTC 2018
SWI/SNF regulates half of its targets without the need of ATP-driven nucleosome remodeling by Brahma.
BMC genomics
Jordán-Pla A,Yu S,Waldholm J,Källman T,Östlund Farrants AK,Visa N
R960-25 was used in Western Blotting to identify Brahma target genes, and investigate the magnitude of the non-catalytic function of Brahma in transcriptional regulation.
Fri May 18 00:00:00 UTC 2018
Novel cooperative pathway of c-Myc and Furin, a pro-protein convertase, in cell proliferation as a therapeutic target in ovarian cancers.
Oncotarget
Hasegawa-Minato J,Toyoshima M,Ishibashi M,Zhang X,Shigeta S,Grandori C,Kitatani K,Yaegashi N
R960-25 was used in Western Blotting to identify Furin as a novel therapeutic target that shows synthetic lethality in c-Myc-driven ovarian cancers via high throughput siRNA screening.
Tue Jan 09 00:00:00 UTC 2018
SUMOylation of ROR-γt inhibits IL-17 expression and inflammation via HDAC2.
Nature communications
Singh AK,Khare P,Obaid A,Conlon KP,Basrur V,DePinho RA,Venuprasad K
R960-25 was used in Chromatin immunoprecipitation to define the molecular mechanisms that govern the transcription factor activity of ROR-γt in the regulation of IL-17.
Tue Oct 30 00:00:00 UTC 2018
Tri-methylation of ATF7IP by G9a/GLP recruits the chromodomain protein MPP8.
Epigenetics & chromatin
Tsusaka T,Kikuchi M,Shimazu T,Suzuki T,Sohtome Y,Akakabe M,Sodeoka M,Dohmae N,Umehara T,Shinkai Y
R960-25 was used in Immunoprecipitation to identify the roles of lysine methylation in non-histone substrates which are targeted by the G9a/GLP complex.
Thu Oct 04 00:00:00 UTC 2018
Identification and functional analysis of SOX10 phosphorylation sites in melanoma.
PloS one
Cronin JC,Loftus SK,Baxter LL,Swatkoski S,Gucek M,Pavan WJ
R960-25 was used in Western Blotting to study phosphorylation as a mechanism employed by melanoma cells to tightly regulate SOX10 expression.
Thu Feb 22 00:00:00 UTC 2018
Hydra Mesoglea Proteome Identifies Thrombospondin as a Conserved Component Active in Head Organizer Restriction.
Scientific reports
Lommel M,Strompen J,Hellewell AL,Balasubramanian GP,Christofidou ED,Thomson AR,Boyle AL,Woolfson DN,Puglisi K,Hartl M,Holstein TW,Adams JC,Özbek S
R960-25 was used in Immunocytochemistry to describe a novel role of thrombospondin as a Wnt signalling target in the basal eumetazoan Hydra.
Mon Aug 06 00:00:00 UTC 2018
The CUE1 domain of the SNF2-like chromatin remodeler SMARCAD1 mediates its association with KRAB-associated protein 1 (KAP1) and KAP1 target genes.
The Journal of biological chemistry
Ding D,Bergmaier P,Sachs P,Klangwart M,Rückert T,Bartels N,Demmers J,Dekker M,Poot RA,Mermoud JE
R960-25 was used in Immunocytochemistry-immunoflourescence to reveal a mechanism that localizes SMARCAD1 to genomic sites through the interaction of SMARCAD1's CUE1 motif with KAP1.
Fri Feb 23 00:00:00 UTC 2018
Epigenetic silencing of SMOC1 in traditional serrated adenoma and colorectal cancer.
Oncotarget
Aoki H,Yamamoto E,Takasawa A,Niinuma T,Yamano HO,Harada T,Matsushita HO,Yoshikawa K,Takagi R,Harada E,Tanaka Y,Yoshida Y,Aoyama T,Eizuka M,Yorozu A,Kitajima H,Kai M,Sawada N,Sugai T,Nakase H,Suzuki H
R960-25 was used in Western Blotting to demonstrate that epigenetic silencing ofSMOC1is associated with TSA development but is rarely observed in SSA/Ps.
Fri Jan 12 00:00:00 UTC 2018
MERIT40-dependent recruitment of tankyrase to damaged DNA and its implication for cell sensitivity to DNA-damaging anticancer drugs.
Oncotarget
Okamoto K,Ohishi T,Kuroiwa M,Iemura SI,Natsume T,Seimiya H
R960-25 was used in Immunocytochemistry-immunoflourescence to study the role of tankyrase in the DNA damage repair response.
Fri Nov 09 00:00:00 UTC 2018
A molecular switch orchestrates enzyme specificity and secretory granule morphology.
Nature communications
Ji S,Samara NL,Revoredo L,Zhang L,Tran DT,Muirhead K,Tabak LA,Ten Hagen KG
R960-25 was used in Western Blotting to identify a gene that influences the structure and shape of secretory granules within the Drosophila salivary gland.
Wed Aug 29 00:00:00 UTC 2018
An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast.
eLife
Lang J,Barber A,Biggins S
R960-25 was used in Western Blotting to demonstrate that the CENP-T pathway becomes essential for viability and Ndc80c recruitment when the Mis12 pathway has Dsn1 phosphorylation defects.
Fri Aug 17 00:00:00 UTC 2018
Balance between senescence and apoptosis is regulated by telomere damage-induced association between p16 and caspase-3.
The Journal of biological chemistry
Panneer Selvam S,Roth BM,Nganga R,Kim J,Cooley MA,Helke K,Smith CD,Ogretmen B
R960-25 was used in Western Blot to suggest that p16 plays a direct role in telomere damage-dependent senescence by limiting apoptosis via binding to caspase-3, revealing a direct link between telomere damage-dependent senescence and apoptosis with regards to aging and cancer.
Fri Jun 22 00:00:00 UTC 2018
The E3 SUMO ligase PIASγ is a novel interaction partner regulating the activity of diabetes associated hepatocyte nuclear factor-1α.
Scientific reports
Kaci A,Keindl M,Solheim MH,Njølstad PR,Bjørkhaug L,Aukrust I
R960-25 was used in Immunoprecipitation to study the SUMOylation of hepatocyte nuclear factor-1α and identify two SUMOylation sites.
Fri Aug 24 00:00:00 UTC 2018
Directed Evolution to Engineer Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface.
Cell chemical biology
Limsakul P,Peng Q,Wu Y,Allen ME,Liang J,Remacle AG,Lopez T,Ge X,Kay BK,Zhao H,Strongin AY,Yang XL,Lu S,Wang Y
R960-25 was used in Flow cytometry/Cell sorting to develop a hybrid fluorescence resonance energy transfer-based biosensor with separated donor and acceptor that assemble in situ.
Thu Apr 19 00:00:00 UTC 2018
A mosquito salivary gland protein partially inhibits Plasmodium sporozoite cell traversal and transmission.
Nature communications
Schleicher TR,Yang J,Freudzon M,Rembisz A,Craft S,Hamilton M,Graham M,Mlambo G,Tripathi AK,Li Y,Cresswell P,Sinnis P,Dimopoulos G,Fikrig E
R960-25 was used in Immunohistochemistry to identify a mosquito salivary gland vector protein with similarity to human gamma interferon inducible thiol reductase.
Wed Jul 25 00:00:00 UTC 2018
The non-classical nuclear import carrier Transportin 1 modulates circadian rhythms through its effect on PER1 nuclear localization.
PLoS genetics
Korge S,Maier B,Brüning F,Ehrhardt L,Korte T,Mann M,Herrmann A,Robles MS,Kramer A
R960-25 was used in Western Blotting to identify that Transportin 1 is essential for normal circadian periods and potentially for redox regulation.
Mon Jan 01 00:00:00 UTC 2018
The non-classical nuclear import carrier Transportin 1 modulates circadian rhythms through its effect on PER1 nuclear localization.
PLoS genetics
Korge S,Maier B,Brüning F,Ehrhardt L,Korte T,Mann M,Herrmann A,Robles MS,Kramer A
R960-25 was used in Western Blotting to identify that Transportin 1 is essential for normal circadian periods and potentially for redox regulation.
Mon Jan 01 00:00:00 UTC 2018
Integrating glycomics and genomics uncovers SLC10A7 as essential factor for bone mineralization by regulating post-Golgi protein transport and glycosylation.
Human molecular genetics
Ashikov A,Abu Bakar N,Wen XY,Niemeijer M,Rodrigues Pinto Osorio G,Brand-Arzamendi K,Hasadsri L,Hansikova H,Raymond K,Vicogne D,Ondruskova N,Simon MEH,Pfundt R,Timal S,Beumers R,Biot C,Smeets R,Kersten M,Huijben K,Linders PTA,van den Bogaart G,van Hijum SAFT,Rodenburg R,van den Heuvel LP,van Spronsen F,Honzik T,Foulquier F,van Scherpenzeel M,Lefeber DJ,Mirjam W,Han B,Helen M,Helen M,Peter VH,Jiddeke VK,Diego M,Lars M,Katja BH,Jozef H,Majid A,Kevin C,Johann TWN
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, Sample Prep to indicate an important role for SLC10A7 in bone mineralization and transport of glycoproteins to the extracellular matrix.
Sat Sep 01 00:00:00 UTC 2018
miRNA-mediated TUSC3 deficiency enhances UPR and ERAD to promote metastatic potential of NSCLC.
Nature communications
Jeon YJ,Kim T,Park D,Nuovo GJ,Rhee S,Joshi P,Lee BK,Jeong J,Suh SS,Grotzke JE,Kim SH,Song J,Sim H,Kim Y,Peng Y,Jeong Y,Garofalo M,Zanesi N,Kim J,Liang G,Nakano I,Cresswell P,Nana-Sinkam P,Cui R,Croce CM
R960-25 was used in Western Blotting to study the roles of tumour suppressor candidate 3 in cancer progression, and the oncogenic role of HRD1-dependent ERAD in cancer metastasis.
Fri Nov 30 00:00:00 UTC 2018
Fumarate hydratase loss promotes mitotic entry in the presence of DNA damage after ionising radiation.
Cell death & disease
Johnson TI,Costa ASH,Ferguson AN,Frezza C
R960-25 was used in Western Blotting to study the effects of a loss of fumarate hydratase on the cellular susceptibility to DNA damage caused by ionising radiation.
Thu Sep 06 00:00:00 UTC 2018
Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.
PLoS genetics
Bowie E,Norris R,Anderson KV,Goetz SC
R960-25 was used in Immunoprecipitation to study the mechanisms by which the spinocerebellar ataxia type 11-associated mutations disrupt Tau tubulin kinase 2 function, and whether they interfere with ciliogenesis.
Sat Dec 01 00:00:00 UTC 2018
MORC2B is essential for meiotic progression and fertility.
PLoS genetics
Shi B,Xue J,Zhou J,Kasowitz SD,Zhang Y,Liang G,Guan Y,Shi Q,Liu M,Sha J,Huang X,Wang PJ
R960-25 was used in Western blotting following immunoprecipitation to outline the role of the MORC2B gene in chromosome synapsis and meiotic recombination in both sexes.
Mon Jan 01 00:00:00 UTC 2018
Heterogeneity of the astrocytic AMPA-receptor transcriptome.
Glia
Mölders A,Koch A,Menke R,Klöcker N
R960-25 was used in Western Blotting to quantify the astrocytic AMPAR transcriptome during brain development and with respect to selected brain regions.
Sat Dec 01 00:00:00 UTC 2018
Nuclear m6A reader YTHDC1 regulates alternative polyadenylation and splicing during mouse oocyte development.
PLoS genetics
Kasowitz SD,Ma J,Anderson SJ,Leu NA,Xu Y,Gregory BD,Schultz RM,Wang PJ
R960-25 was used in Immunoprecipitation to study the role of the nuclear m6A reader, YTHDC1, in the oocyte nucleus throughout foetal development.
Tue May 01 00:00:00 UTC 2018
Nuclear m6A reader YTHDC1 regulates alternative polyadenylation and splicing during mouse oocyte development.
PLoS genetics
Kasowitz SD,Ma J,Anderson SJ,Leu NA,Xu Y,Gregory BD,Schultz RM,Wang PJ
R960-25 was used in Immunoprecipitation to study the role of the nuclear m6A reader, YTHDC1, in the oocyte nucleus throughout foetal development.
Tue May 01 00:00:00 UTC 2018
Autopolysialylation of polysialyltransferases is required for polysialylation and polysialic acid chain elongation on select glycoprotein substrates.
The Journal of biological chemistry
Bhide GP,Zapater JL,Colley KJ
R960-25 was used in Western Blotting to compare the polybasic region sequence requirements for neural cell adhesion molecule, neuropilin-2, and synaptic cell adhesion molecule 1 for polysialylation by their respective polysialyltransferases.
Fri Jan 12 00:00:00 UTC 2018
An approach to suppress the evolution of resistance in BRAFV600E-mutant cancer.
Nature medicine
Xue Y,Martelotto L,Baslan T,Vides A,Solomon M,Mai TT,Chaudhary N,Riely GJ,Li BT,Scott K,Cechhi F,Stierner U,Chadalavada K,de Stanchina E,Schwartz S,Hembrough T,Nanjangud G,Berger MF,Nilsson J,Lowe SW,Reis-Filho JS,Rosen N,Lito P
R960-25 was used in Western Blotting to study how gene amplification can be acquired and expanded through parallel evolution, enabling tumors to adapt while maintaining their intratumoral heterogeneity.
Tue Aug 01 00:00:00 UTC 2017
Tumours with class 3 BRAF mutants are sensitive to the inhibition of activated RAS.
Nature
Yao Z,Yaeger R,Rodrik-Outmezguine VS,Tao A,Torres NM,Chang MT,Drosten M,Zhao H,Cecchi F,Hembrough T,Michels J,Baumert H,Miles L,Campbell NM,de Stanchina E,Solit DB,Barbacid M,Taylor BS,Rosen N
R960-25 was used in Western Blotting to define three distinct functional classes of BRAF mutants in human tumours.
Thu Aug 10 00:00:00 UTC 2017
Identification of CMTM6 and CMTM4 as PD-L1 protein regulators.
Nature
Mezzadra R,Sun C,Jae LT,Gomez-Eerland R,de Vries E,Wu W,Logtenberg MEW,Slagter M,Rozeman EA,Hofland I,Broeks A,Horlings HM,Wessels LFA,Blank CU,Xiao Y,Heck AJR,Borst J,Brummelkamp TR,Schumacher TNM
R960-25 was used in Western blotting to identify CMTM6 and CMTM4 as regulators of PD-L1 expression across a range of cell types, suggesting their potential as therapeutic targets.
Thu Sep 07 00:00:00 UTC 2017
Ligand and Target Discovery by Fragment-Based Screening in Human Cells.
Cell
Parker CG,Galmozzi A,Wang Y,Correia BE,Sasaki K,Joslyn CM,Kim AS,Cavallaro CL,Lawrence RM,Johnson SR,Narvaiza I,Saez E,Cravatt BF
R960-25 was used in Western Blot to produce a proteome-wide map of protein ligandability, allowing for the coordinated discovery of bioactive small molecules and their molecular targets.
Thu Jan 26 00:00:00 UTC 2017
Dynamics of BAF-Polycomb complex opposition on heterochromatin in normal and oncogenic states.
Nature genetics
Kadoch C,Williams RT,Calarco JP,Miller EL,Weber CM,Braun SM,Pulice JL,Chory EJ,Crabtree GR
R960-25 was used in Western Blotting to define a mechanistic sequence underlying the resolution and formation of facultative heterochromatin.
Wed Feb 01 00:00:00 UTC 2017
NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program.
Nature cell biology
Hiraike Y,Waki H,Yu J,Nakamura M,Miyake K,Nagano G,Nakaki R,Suzuki K,Kobayashi H,Yamamoto S,Sun W,Aoyama T,Hirota Y,Ohno H,Oki K,Yoneda M,White AP,Tseng YH,Cypess AM,Larsen TJ,Jespersen NZ,Scheele C,Tsutsumi S,Aburatani H,Yamauchi T,Kadowaki T
R960-25 was used in Western Blotting to identify nuclear factor I-A as a transcriptional regulator of the brown fat gene programme.
Fri Sep 01 00:00:00 UTC 2017
A Multi-step Transcriptional and Chromatin State Cascade Underlies Motor Neuron Programming from Embryonic Stem Cells.
Cell stem cell
Velasco S,Ibrahim MM,Kakumanu A,Garipler G,Aydin B,Al-Sayegh MA,Hirsekorn A,Abdul-Rahman F,Satija R,Ohler U,Mahony S,Mazzoni EO
R960-25 was used in Immunocytochemistry to investigate the underlying mechanisms of successful terminal cell fate programming by looking at gene expression, chromatin states, and transcription factors binding during the uniquely efficient Ngn2, Isl1, and Lhx3 motor neuron programming pathway.
Thu Feb 02 00:00:00 UTC 2017
Haploinsufficiency for DNA methyltransferase 3A predisposes hematopoietic cells to myeloid malignancies.
The Journal of clinical investigation
Cole CB,Russler-Germain DA,Ketkar S,Verdoni AM,Smith AM,Bangert CV,Helton NM,Guo M,Klco JM,O'Laughlin S,Fronick C,Fulton R,Chang GS,Petti AA,Miller CA,Ley TJ
R960-25 was used in Western Blotting to examine the correlation between haploinsufficiency for DNA methyltransferase 3A and myeloid malignancies.
Mon Oct 02 00:00:00 UTC 2017
RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs.
The Journal of clinical investigation
Wang J,Rajbhandari P,Damianov A,Han A,Sallam T,Waki H,Villanueva CJ,Lee SD,Nielsen R,Mandrup S,Reue K,Young SG,Whitelegge J,Saez E,Black DL,Tontonoz P
R960-25 was used in Western Blotting to study the effect of the RNA-binding protein PSPC1 on adipogenesis and mature adipocyte function in mice.
Wed Mar 01 00:00:00 UTC 2017
LSM12 and ME31B/DDX6 Define Distinct Modes of Posttranscriptional Regulation by ATAXIN-2 Protein Complex in Drosophila Circadian Pacemaker Neurons.
Molecular cell
Lee J,Yoo E,Lee H,Park K,Hur JH,Lim C
R960-25 was used in Immunoprecipitation to demonstrate that LSM12 and ME31B/DDX6 are two ATAXIN-2-associating factors crucial for sustaining circadian rhythms.
Thu Apr 06 00:00:00 UTC 2017
Structural Mechanism for Modulation of Synaptic Neuroligin-Neurexin Signaling by MDGA Proteins.
Neuron
Elegheert J,Cvetkovska V,Clayton AJ,Heroven C,Vennekens KM,Smukowski SN,Regan MC,Jia W,Smith AC,Furukawa H,Savas JN,de Wit J,Begbie J,Craig AM,Aricescu AR
R960-25 was used in Immunocytochemistry to illustrate a potentially brain-wide regulatory mechanism for neuroligin-neurexin signalling modulation.
Wed Aug 16 00:00:00 UTC 2017
Nipbl Interacts with Zfp609 and the Integrator Complex to Regulate Cortical Neuron Migration.
Neuron
van den Berg DLC,Azzarelli R,Oishi K,Martynoga B,Urbán N,Dekkers DHW,Demmers JA,Guillemot F
R960-25 was used in Immunocytochemistry to provide mechanistic insight for the role of Nipbl in the neurological defects associated with Cornelia de Lange syndrome.
Wed Jan 18 00:00:00 UTC 2017
Nipbl Interacts with Zfp609 and the Integrator Complex to Regulate Cortical Neuron Migration.
Neuron
van den Berg DLC,Azzarelli R,Oishi K,Martynoga B,Urbán N,Dekkers DHW,Demmers JA,Guillemot F
R960-25 was used in Immunocytochemistry to provide mechanistic insight for the role of Nipbl in the neurological defects associated with Cornelia de Lange syndrome.
Wed Jan 18 00:00:00 UTC 2017
Divergent Connectivity of Homologous Command-like Neurons Mediates Segment-Specific Touch Responses in Drosophila.
Neuron
Takagi S,Cocanougher BT,Niki S,Miyamoto D,Kohsaka H,Kazama H,Fetter RD,Truman JW,Zlatic M,Cardona A,Nose A
R960-25 was used in Immunohistochemistry-immunofluorescence to investigate how somatosensory inputs on different body segments are linked to distinct motor outputs in Drosophila larvae.
Wed Dec 20 00:00:00 UTC 2017
MTCL1 plays an essential role in maintaining Purkinje neuron axon initial segment.
The EMBO journal
Satake T,Yamashita K,Hayashi K,Miyatake S,Tamura-Nakano M,Doi H,Furuta Y,Shioi G,Miura E,Takeo YH,Yoshida K,Yahikozawa H,Matsumoto N,Yuzaki M,Suzuki A
Tue May 02 00:00:00 UTC 2017
Xenopus laevis M18BP1 Directly Binds Existing CENP-A Nucleosomes to Promote Centromeric Chromatin Assembly.
Developmental cell
French BT,Westhorpe FG,Limouse C,Straight AF
R960-25 was used in Immunocytochemistry-immunoflourescence to define cellular mechanisms for recruiting CENP-A assembly factors to existing CENP-A nucleosomes for the epigenetic inheritance of centromeres.
Mon Jul 24 00:00:00 UTC 2017
PWP1 Mediates Nutrient-Dependent Growth Control through Nucleolar Regulation of Ribosomal Gene Expression.
Developmental cell
Liu Y,Mattila J,Ventelä S,Yadav L,Zhang W,Lamichane N,Sundström J,Kauko O,Grénman R,Varjosalo M,Westermarck J,Hietakangas V
R960-25 was used in Western Blotting to study the role of PWP1 as a chromatin-associated regulator of developmental growth with a conserved role in RNA polymerase I-mediated rRNA transcription.
Mon Oct 23 00:00:00 UTC 2017
CycD/Cdk4 and Discontinuities in Dpp Signaling Activate TORC1 in the Drosophila Wing Disc.
Developmental cell
Romero-Pozuelo J,Demetriades C,Schroeder P,Teleman AA
R960-25 was used in Immunoprecipitation to characterise the spatial distribution of TORC1 activity in a developing Drosophila wing disc.
Mon Aug 21 00:00:00 UTC 2017
Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B.
Autophagy
Lennemann NJ,Coyne CB
R960-25 was used in Western Blotting to identify the endoplasmic reticulum-localised reticulophagy receptor, FAM134B, as a host cell restriction factor for both dengue virus and Zika virus.
Wed Feb 01 00:00:00 UTC 2017
Foxp1 regulation of neonatal vocalizations via cortical development.
Genes & development
Usui N,Araujo DJ,Kulkarni A,Co M,Ellegood J,Harper M,Toriumi K,Lerch JP,Konopka G
R960-25 was used in Western Blotting to elucidate the contribution of FOXP1-mediated signalling to brain development and, in particular, neocortical development.
Sun Oct 15 00:00:00 UTC 2017
Dynein light chain 1 induces assembly of large Bim complexes on mitochondria that stabilize Mcl-1 and regulate apoptosis.
Genes & development
Singh PK,Roukounakis A,Frank DO,Kirschnek S,Das KK,Neumann S,Madl J,Römer W,Zorzin C,Borner C,Haimovici A,Garcia-Saez A,Weber A,Häcker G
R960-25 was used in Western Blotting to study the role of large protein complexes containing dyenin light chain 1 in the regulation of Bim.
Fri Sep 01 00:00:00 UTC 2017
Cap-binding protein 4EHP effects translation silencing by microRNAs.
Proceedings of the National Academy of Sciences of the United States of America
Chapat C,Jafarnejad SM,Matta-Camacho E,Hesketh GG,Gelbart IA,Attig J,Gkogkas CG,Alain T,Stern-Ginossar N,Fabian MR,Gingras AC,Duchaine TF,Sonenberg N
R960-25 was used in Immunoprecipitation to show that 4EHP competes with eIF4E for binding to 4E-T, and this interaction increases the affinity of 4EHP for the cap.
Tue May 23 00:00:00 UTC 2017
Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.
Cell systems
Yadav L,Tamene F,Göös H,van Drogen A,Katainen R,Aebersold R,Gstaiger M,Varjosalo M
R960-25 was used in Western blotting following immunoprecipitation to study the protein-protein interactions and dynamics of 54 human phosphatase.
Wed Apr 26 00:00:00 UTC 2017
Fine Mapping and Functional Analysis Reveal a Role of SLC22A1 in Acylcarnitine Transport.
American journal of human genetics
Kim HI,Raffler J,Lu W,Lee JJ,Abbey D,Saleheen D,Rabinowitz JD,Bennett MJ,Hand NJ,Brown C,Rader DJ
R960-25 was used in Western Blotting to assess the role of SLC22A1 and its variants in acylcarnitine transport.
Thu Oct 05 00:00:00 UTC 2017
Suppressed ubiquitination of Nrf2 by p47phox contributes to Nrf2 activation.
Free radical biology & medicine
Ha Kim K,Sadikot RT,Yeon Lee J,Jeong HS,Oh YK,Blackwell TS,Joo M
R960-25 was used in Western Blotting to investigate whether NADPH oxidase components participate in the direct activation of Nrf2, contributing to protection from reactive oxygen species.
Fri Dec 01 00:00:00 UTC 2017
Three-tier regulation of cell number plasticity by neurotrophins and Tolls in Drosophila.
The Journal of cell biology
Foldi I,Anthoney N,Harrison N,Gangloff M,Verstak B,Nallasivan MP,AlAhmed S,Zhu B,Phizacklea M,Losada-Perez M,Moreira M,Gay NJ,Hidalgo A
R960-25 was used in Western Blotting to demonstrate that DNTs and Tolls can switch from promoting cell survival to death in the CNS via a three-tier mechanism.
Mon May 01 00:00:00 UTC 2017
Protein Tyrosine Phosphatase δ Mediates the Sema3A-Induced Cortical Basal Dendritic Arborization through the Activation of Fyn Tyrosine Kinase.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Nakamura F,Okada T,Shishikura M,Uetani N,Taniguchi M,Yagi T,Iwakura Y,Ohshima T,Goshima Y,Strittmatter SM
R960-25 was used in Western Blotting to show that LAR class PTPs participate in Semaphorin signaling in vivo.
Wed Jul 26 00:00:00 UTC 2017
The Intellectual Disability and Schizophrenia Associated Transcription Factor TCF4 Is Regulated by Neuronal Activity and Protein Kinase A.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Sepp M,Vihma H,Nurm K,Urb M,Page SC,Roots K,Hark A,Maher BJ,Pruunsild P,Timmusk T
R960-25 was used in Western Blotting to identify a novel role for transcription factor 4 (TCF4) as a neuronal-activity-regulated transcription factor, offering a novel perspective on the association of TCF4 with cognitive disorders.
Wed Oct 25 00:00:00 UTC 2017
Conserved Interaction of Lentiviral Vif Molecules with HIV-1 Gag and Differential Effects of Species-Specific Vif on Virus Production.
Journal of virology
Zheng W,Ling L,Li Z,Wang H,Rui Y,Gao W,Wang S,Su X,Wei W,Yu XF
R960-25 was used in Western Blot to demonstrate that diverse lentiviral Vif molecules maintain the ability to interact with the human immunodeficiency virus type 1 Gag precursor polyprotein.
Sat Apr 01 00:00:00 UTC 2017
Loss of the Human Cytomegalovirus US16 Protein Abrogates Virus Entry into Endothelial and Epithelial Cells by Reducing the Virion Content of the Pentamer.
Journal of virology
Luganini A,Cavaletto N,Raimondo S,Geuna S,Gribaudo G
R960-25 was used in Immunofluorescence to suggest pUS16 as a viral regulatory protein important for shaping virion composition which influences human cytomegalovirus cell tropism.
Thu Jun 01 00:00:00 UTC 2017
Protein-Protein Interaction Among the FoxP Family Members and their Regulation of Two Target Genes, VLDLR and CNTNAP2 in the Zebra Finch Song System.
Frontiers in molecular neuroscience
Mendoza E,Scharff C
R960-25 was used in Western Blotting to exemplify the potential for complex transcriptional regulation of FoxP1/2/4, highlighting the need for future functional studies dissecting their differential regulation in the brain.
Wed Sep 30 00:00:00 UTC 2020
Pericellular regulation of prostate cancer expressed kallikrein-related peptidases and matrix metalloproteinases by cell surface serine proteases.
American journal of cancer research
Reid JC,Matsika A,Davies CM,He Y,Broomfield A,Bennett NC,Magdolen V,Srinivasan B,Clements JA,Hooper JD
R960-25 was used in Immunocytochemistry to identify a novel pericellular network of proteases that are elevated and co-expressed in prostate cancer.
Thu Oct 01 00:00:00 UTC 2020
Pericellular regulation of prostate cancer expressed kallikrein-related peptidases and matrix metalloproteinases by cell surface serine proteases.
American journal of cancer research
Reid JC,Matsika A,Davies CM,He Y,Broomfield A,Bennett NC,Magdolen V,Srinivasan B,Clements JA,Hooper JD
R960-25 was used in Immunocytochemistry to identify a novel pericellular network of proteases that are elevated and co-expressed in prostate cancer.
Thu Oct 01 00:00:00 UTC 2020
Pericellular regulation of prostate cancer expressed kallikrein-related peptidases and matrix metalloproteinases by cell surface serine proteases.
American journal of cancer research
Reid JC,Matsika A,Davies CM,He Y,Broomfield A,Bennett NC,Magdolen V,Srinivasan B,Clements JA,Hooper JD
R960-25 was used in Immunocytochemistry to identify a novel pericellular network of proteases that are elevated and co-expressed in prostate cancer.
Thu Oct 01 00:00:00 UTC 2020
The Fifth Epidermal Growth Factor-like Region of Thrombomodulin Alleviates Murine Graft-versus-Host Disease in a G-Protein Coupled Receptor 15 Dependent Manner.
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation
Pan B,Wang X,Kojima S,Nishioka C,Yokoyama A,Honda G,Xu K,Ikezoe T
R960-25 was used in Immunoprecipitation to identify G-protein coupled receptor 15 expressed on T cells as a receptor/sensor of thrombomodulin.
Mon May 01 00:00:00 UTC 2017
The Fifth Epidermal Growth Factor-like Region of Thrombomodulin Alleviates Murine Graft-versus-Host Disease in a G-Protein Coupled Receptor 15 Dependent Manner.
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation
Pan B,Wang X,Kojima S,Nishioka C,Yokoyama A,Honda G,Xu K,Ikezoe T
R960-25 was used in Immunoprecipitation to identify G-protein coupled receptor 15 expressed on T cells as a receptor/sensor of thrombomodulin.
Mon May 01 00:00:00 UTC 2017
Integrated STAT3 and Ikaros Zinc Finger Transcription Factor Activities Regulate Bcl-6 Expression in CD4+ Th Cells.
Journal of immunology (Baltimore, Md. : 1950)
Read KA,Powell MD,Baker CE,Sreekumar BK,Ringel-Scaia VM,Bachus H,Martin RE,Cooley ID,Allen IC,Ballesteros-Tato A,Oestreich KJ
R960-25 was used in Western Blotting to identify the Ikaros zinc finger transcription factors, Aiolos and Ikaros, as novel regulators of Bcl-6.
Sun Oct 01 00:00:00 UTC 2017
RING finger protein 113A regulates C-X-C chemokine receptor type 4 stability and signaling.
American journal of physiology. Cell physiology
Lear T,Dunn SR,McKelvey AC,Mir A,Evankovich J,Chen BB,Liu Y
R960-25 was used in Western Blotting to provide new understanding of HIV immunity and neutrophil activation and motility regulated by CXCR4.
Wed Nov 01 00:00:00 UTC 2017
Integrin α8 and Pcdh15 act as a complex to regulate cilia biogenesis in sensory cells.
Journal of cell science
Goodman L,Zallocchi M
R960-25 was used in Immunohistochemistry-immunofluorescence to identify an integrin α8 and protocadherin-15a ciliary complex in neuromast hair cells in a zebrafish model.
Wed Nov 01 00:00:00 UTC 2017
Wnt proteins synergize to activate β-catenin signaling.
Journal of cell science
Alok A,Lei Z,Jagannathan NS,Kaur S,Harmston N,Rozen SG,Tucker-Kellogg L,Virshup DM
R960-25 was used in Western Blotting to find that multiple Wnt combinations can synergistically activate β-catenin signalling downstream of β-catenin stabilization, correlated with increased lysine acetylation of β-catenin.
Mon May 01 00:00:00 UTC 2017
G0S2 represses PI3K/mTOR signaling and increases sensitivity to PI3K/mTOR pathway inhibitors in breast cancer.
Cell cycle (Georgetown, Tex.)
Yim CY,Bikorimana E,Khan E,Warzecha JM,Shin L,Rodriguez J,Dmitrovsky E,Freemantle SJ,Spinella MJ
R960-25 was used in Western Blotting to determine potential mechanisms of G0/G1 switch gene 2 anti-oncogenic activity.
Tue Aug 07 00:00:00 UTC 2018
Hepatic Tm6sf2 overexpression affects cellular ApoB-trafficking, plasma lipid levels, hepatic steatosis and atherosclerosis.
Human molecular genetics
Ehrhardt N,Doche ME,Chen S,Mao HZ,Walsh MT,Bedoya C,Guindi M,Xiong W,Ignatius Irudayam J,Iqbal J,Fuchs S,French SW,Mahmood Hussain M,Arditi M,Arumugaswami V,Péterfy M
R960-25 was used in Western Blotting to investigate the role of human transmembrane 6 superfamily member 2 in metabolic homeostasis.
Sat Jul 15 00:00:00 UTC 2017
Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions.
Human molecular genetics
Saha M,Mitsuhashi S,Jones MD,Manko K,Reddy HM,Bruels CC,Cho KA,Pacak CA,Draper I,Kang PB
R960-25 was used in Flow cytometry/Cell sorting to demonstrate that short hairpin RNA-mediated knockdown of Megf10 leads to impaired proliferation and migration of C2C12 cells.
Tue Aug 01 00:00:00 UTC 2017
Novel nesprin-1 mutations associated with dilated cardiomyopathy cause nuclear envelope disruption and defects in myogenesis.
Human molecular genetics
Zhou C,Li C,Zhou B,Sun H,Koullourou V,Holt I,Puckelwartz MJ,Warren DT,Hayward R,Lin Z,Zhang L,Morris GE,McNally EM,Shackleton S,Rao L,Shanahan CM,Zhang Q
R960-25 was used in Immunocytochemistry-immunoflourescence to identify novel nesprin-1 mutations associated with dilated cardiomyopathy which cause nuclear envelope disruption and defects in myogenesis.
Thu Jun 15 00:00:00 UTC 2017
Dual Mechanism of Rag Gene Repression by c-Myb during Pre-B Cell Proliferation.
Molecular and cellular biology
Timblin GA,Xie L,Tjian R,Schlissel MS
R960-25 was used in Chromatin immunoprecipitation to identify two mechanisms by which c-Myb represses Rag transcription.
Thu Jun 15 00:00:00 UTC 2017
MTORC1 Regulates both General Autophagy and Mitophagy Induction after Oxidative Phosphorylation Uncoupling.
Molecular and cellular biology
Bartolomé A,García-Aguilar A,Asahara SI,Kido Y,Guillén C,Pajvani UB,Benito M
R960-25 was used in Western Blotting to investigate whether mechanistic target of rapamycin complex 1 specifically regulates autophagic clearance of damaged mitochondria.
Fri Dec 01 00:00:00 UTC 2017
The N-Terminal Part of the Dishevelled DEP Domain Is Required for Wnt/β-Catenin Signaling in Mammalian Cells.
Molecular and cellular biology
Paclíková P,Bernatík O,Radaszkiewicz TW,Bryja V
R960-25 was used in Immunofluorescence-cell culture cells to provide evidence that DVL is indispensable for the phosphorylation of LRP6 and downstream signaling after activation by the Wnt3a ligand in mammalian cells.
Fri Sep 15 00:00:00 UTC 2017
F-Box Protein XREP-4 Is a New Regulator of the Oxidative Stress Response in Caenorhabditis elegans.
Genetics
Wu CW,Wang Y,Choe KP
R960-25 was used in Western Blotting to study the complex signalling pathway mediated by the Skinhead family member-1 transcription factor in Caenorhabditis elegans.
Thu Jun 01 00:00:00 UTC 2017
Long-term oral kinetin does not protect against α-synuclein-induced neurodegeneration in rodent models of Parkinson's disease.
Neurochemistry international
Orr AL,Rutaganira FU,de Roulet D,Huang EJ,Hertz NT,Shokat KM,Nakamura K
R960-25 was used in Immunohistochemistry-immunofluorescence to study the effect of PINK1 deficiency on Parkinson's disease.
Sun Oct 01 00:00:00 UTC 2017
APOBEC3A intratumoral DNA electroporation in mice.
Gene therapy
Kostrzak A,Caval V,Escande M,Pliquet E,Thalmensi J,Bestetti T,Julithe M,Fiette L,Huet T,Wain-Hobson S,Langlade-Demoyen P
R96025 was used in immunocytochemistry and western blot to assess the antitumor efficacy of APOBEC3A
Wed Feb 01 00:00:00 UTC 2017
Meiosis-specific proteins MEIOB and SPATA22 cooperatively associate with the single-stranded DNA-binding replication protein A complex and DNA double-strand breaks.
Biology of reproduction
Xu Y,Greenberg RA,Schonbrunn E,Wang PJ
R960-25 was used in Western blotting following immunoprecipitation to study the molecular mechanisms of MEIOB and its interactions with spermatogenesis associated 22 and RPA.
Mon May 01 00:00:00 UTC 2017
Meiosis-specific proteins MEIOB and SPATA22 cooperatively associate with the single-stranded DNA-binding replication protein A complex and DNA double-strand breaks.
Biology of reproduction
Xu Y,Greenberg RA,Schonbrunn E,Wang PJ
R960-25 was used in Western blotting following immunoprecipitation to study the molecular mechanisms of MEIOB and its interactions with spermatogenesis associated 22 and RPA.
Mon May 01 00:00:00 UTC 2017
Ceramide phosphoethanolamine synthase SMSr is a target of caspase-6 during apoptotic cell death.
Bioscience reports
Cabukusta B,Nettebrock NT,Kol M,Hilderink A,Tafesse FG,Holthuis JCM
R960-25 was used in Western Blotting and ICC/IF to demonstrate that SMSr is a target of the apoptotic machinery.
Thu Aug 31 00:00:00 UTC 2017
Ceramide phosphoethanolamine synthase SMSr is a target of caspase-6 during apoptotic cell death.
Bioscience reports
Cabukusta B,Nettebrock NT,Kol M,Hilderink A,Tafesse FG,Holthuis JCM
R960-25 was used in Western Blotting and ICC/IF to demonstrate that SMSr is a target of the apoptotic machinery.
Thu Aug 31 00:00:00 UTC 2017
An interaction study in mammalian cells demonstrates weak binding of HSPB2 to BAG3, which is regulated by HSPB3 and abrogated by HSPB8.
Cell stress & chaperones
Morelli FF,Mediani L,Heldens L,Bertacchini J,Bigi I,Carrà AD,Vinet J,Carra S
R960-25 was used in Western Blotting to support the interpretation that small heat shock protein (HSP)B8-BAG3 is the preferred HSP interaction.
Sat Jul 01 00:00:00 UTC 2017
IgG light chain-independent secretion of heavy chain dimers: consequence for therapeutic antibody production and design.
The Biochemical journal
Stoyle CL,Stephens PE,Humphreys DP,Heywood S,Cain K,Bulleid NJ
R960-25 was used in Western Blotting to show that the secreted heavy-chain dimers contain folded CH1 domains and contribute to the heterogeneity of antibody species secreted during the expression of therapeutic antibodies.
Thu Sep 07 00:00:00 UTC 2017
An interaction domain in human SAMD9 is essential for myxoma virus host-range determinant M062 antagonism of host anti-viral function.
Virology
Nounamo B,Li Y,O'Byrne P,Kearney AM,Khan A,Liu J
R960-25 was used in Immunoprecipitation to reveal the first molecular insight into viral M062-dependent mechanisms that suppress human SAMD9-associated antiviral function.
Wed Mar 01 00:00:00 UTC 2017
Spatio-temporal pattern of neuronal differentiation in the Drosophila visual system: A user's guide to the dynamic morphology of the developing optic lobe.
Developmental biology
Ngo KT,Andrade I,Hartenstein V
R960-25 was used in Immunohistochemistry to analyse the emergence of the discrete layers of the medulla and lobula complex using DN-cadherin, Brp.
Tue Aug 01 00:00:00 UTC 2017
Fic-mediated deAMPylation is not dependent on homodimerization and rescues toxic AMPylation in flies.
The Journal of biological chemistry
Casey AK,Moehlman AT,Zhang J,Servage KA,Krämer H,Orth K
R960-25 was used in Western Blotting to demonstrate that excessive AMPylation by a constitutively active FicE247G mutant is lethal in Drosophila.
Fri Dec 22 00:00:00 UTC 2017
Coordinate regulation of stress signaling and epigenetic events by Acss2 and HIF-2 in cancer cells.
PloS one
Chen R,Xu M,Nagati J,Garcia JA
R960-25 was used in Western Blotting to conclude that an acetate switch in mammals links nutrient intake and stress signaling with tumor growth and metastasis.
Tue Feb 06 00:00:00 UTC 2018
IL-4 driven transcription factor FoxQ1 is expressed by monocytes in atopic dermatitis and stimulates monocyte migration.
Scientific reports
Ovsiy I,Riabov V,Manousaridis I,Michel J,Moganti K,Yin S,Liu T,Sticht C,Kremmer E,Harmsen MC,Goerdt S,Gratchev A,Kzhyshkowska J
R960-25 was used in Immunocytochemistry-immunoflourescence to indicate that FoxQ1 stimulates monocyte motility, increases pro-inflammatory potential, and directs monocyte migration towards MCP-1 causing monocyte influx into inflammatory sites.
Mon Dec 04 00:00:00 UTC 2017
The Bitter Taste Receptor TAS2R16 Achieves High Specificity and Accommodates Diverse Glycoside Ligands by using a Two-faced Binding Pocket.
Scientific reports
Thomas A,Sulli C,Davidson E,Berdougo E,Phillips M,Puffer BA,Paes C,Doranz BJ,Rucker JB
R960-25 was used in Flow cytometry/Cell sorting to characterise the residues that contribute to ligand specificity in the bitter taste receptor, TAS2R16.
Thu Aug 10 00:00:00 UTC 2017
Inhibiting clathrin-mediated endocytosis of the leucine-rich G protein-coupled receptor-5 diminishes cell fitness.
The Journal of biological chemistry
Snyder JC,Rochelle LK,Ray C,Pack TF,Bock CB,Lubkov V,Lyerly HK,Waggoner AS,Barak LS,Caron MG
R960-25 was used in Western Blotting to provide proof-of-concept genetic evidence that blocking the clathrin-mediated endocytosis of leucine-rich G protein-coupled receptor-5 could be used to pharmacologically control cell behaviour.
Fri Apr 28 00:00:00 UTC 2017
The cytoplasmic nuclear receptor RARγ controls RIP1 initiated cell death when cIAP activity is inhibited.
Nature communications
Xu Q,Jitkaew S,Choksi S,Kadigamuwa C,Qu J,Choe M,Jang J,Liu C,Liu ZG
R960-25 was used in Western Blotting to describe the role of the cytoplasmic nuclear receptor RARγ in RIP1-, but not TRADD-, initiated cell death response to death factor treatments.
Mon Sep 04 00:00:00 UTC 2017
The EMSY threonine 207 phospho-site is required for EMSYdriven suppression of DNA damage repair.
Oncotarget
Jelinic P,Eccles LA,Tseng J,Cybulska P,Wielgos M,Powell SN,Levine DA
R960-25 was used in Western Blotting to suggest that EMSY-driven suppression of DNA damage repair is a BRCA2-independent process.
Tue Feb 21 00:00:00 UTC 2017
Phosphorylation of the type II transmembrane serine protease, TMPRSS13, in hepatocyte growth factor activator inhibitor-1 and -2-mediated cell-surface localization.
The Journal of biological chemistry
Murray AS,Varela FA,Hyland TE,Schoenbeck AJ,White JM,Tanabe LM,Todi SV,List K
R960-25 was used in Western Blotting to investigate the activation, inhibition, post-translational modification, and localisation of TMPRSS13.
Fri Sep 08 00:00:00 UTC 2017
DYRK1B blocks canonical and promotes non-canonical Hedgehog signaling through activation of the mTOR/AKT pathway.
Oncotarget
Singh R,Dhanyamraju PK,Lauth M
R960-25 was used in Western Blotting to identify the DYRK1B kinase as a mediator between Hedghog signaling and mTOR/AKT activation.
Tue Jan 03 00:00:00 UTC 2017
Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba.
Scientific reports
Biscontin A,Wallach T,Sales G,Grudziecki A,Janke L,Sartori E,Bertolucci C,Mazzotta G,De Pittà C,Meyer B,Kramer A,Costa R
R960-25 was used in Immunoprecipitation to characterise the 20 circadian clock components in the Antarctic krill, Euphausia superba.
Mon Dec 18 00:00:00 UTC 2017
LFA-1 activates focal adhesion kinases FAK1/PYK2 to generate LAT-GRB2-SKAP1 complexes that terminate T-cell conjugate formation.
Nature communications
Raab M,Lu Y,Kohler K,Smith X,Strebhardt K,Rudd CE
R960-25 was used in Immunoprecipitation to outline a new model for lymphocyte function-associated antigen 1 in which the integrin can mediate both adhesion and de-adhesion events dependent on receptor cross-linking.
Wed Jul 12 00:00:00 UTC 2017
LFA-1 activates focal adhesion kinases FAK1/PYK2 to generate LAT-GRB2-SKAP1 complexes that terminate T-cell conjugate formation.
Nature communications
Raab M,Lu Y,Kohler K,Smith X,Strebhardt K,Rudd CE
R960-25 was used in Immunoprecipitation to outline a new model for lymphocyte function-associated antigen 1 in which the integrin can mediate both adhesion and de-adhesion events dependent on receptor cross-linking.
Wed Jul 12 00:00:00 UTC 2017
Cleavage of the SUN-domain protein Mps3 at its N-terminus regulates centrosome disjunction in budding yeast meiosis.
PLoS genetics
Li P,Jin H,Koch BA,Abblett RL,Han X,Yates JR,Yu HG
R960-25 was used in Western Blotting to provide a genetic mechanism for the regulation of SUN-domain protein-mediated activities.
Thu Jun 01 00:00:00 UTC 2017
SRSF1-dependent nuclear export inhibition of C9ORF72 repeat transcripts prevents neurodegeneration and associated motor deficits.
Nature communications
Hautbergue GM,Castelli LM,Ferraiuolo L,Sanchez-Martinez A,Cooper-Knock J,Higginbottom A,Lin YH,Bauer CS,Dodd JE,Myszczynska MA,Alam SM,Garneret P,Chandran JS,Karyka E,Stopford MJ,Smith EF,Kirby J,Meyer K,Kaspar BK,Isaacs AM,El-Khamisy SF,De Vos KJ,Ning K,Azzouz M,Whitworth AJ,Shaw PJ
R960-25 was used in Immunofluorescence to show that repeat RNA-sequestration of SRSF1 triggers the NXF1-dependent nuclear export of C9ORF72 transcripts retaining expanded hexanucleotide repeats and reveal a novel promising therapeutic target for neuroprotection.
Wed Jul 05 00:00:00 UTC 2017
CIB2 interacts with TMC1 and TMC2 and is essential for mechanotransduction in auditory hair cells.
Nature communications
Giese APJ,Tang YQ,Sinha GP,Bowl MR,Goldring AC,Parker A,Freeman MJ,Brown SDM,Riazuddin S,Fettiplace R,Schafer WR,Frolenkov GI,Ahmed ZM
R960-25 was used in Western blotting following immunoprecipitation to study the exact functions of calcium and integrin-binding protein 2.
Thu Jun 29 00:00:00 UTC 2017
CDH1 regulates E2F1 degradation in response to differentiation signals in keratinocytes.
Oncotarget
Singh RK,Dagnino L
R960-25 was used in Western Blotting to identify novel regulatory mechanisms that jointly modulate post-translational modifications and downregulation of E2F1, which are necessary for proper epidermal keratinocyte differentiation.
Tue Jan 17 00:00:00 UTC 2017
Systematic and Quantitative Assessment of Hydrogen Peroxide Reactivity With Cysteines Across Human Proteomes.
Molecular & cellular proteomics : MCP
Fu L,Liu K,Sun M,Tian C,Sun R,Morales Betanzos C,Tallman KA,Porter NA,Yang Y,Guo D,Liebler DC,Yang J
R960-25 was used in Western Blotting to develop a chemoproteomic platform to systematically and quantitatively analyse the reactivity of thousands of cysteines toward hydrogen peroxide in human cells.
Sun Oct 01 00:00:00 UTC 2017
The E3 ubiquitin ligase Siah1 regulates adrenal gland organization and aldosterone secretion.
JCI insight
Scortegagna M,Berthon A,Settas N,Giannakou A,Garcia G,Li JL,James B,Liddington RC,Vilches-Moure JG,Stratakis CA,Ronai ZA
R960-25 was used in Western Blotting to identify a role for the ubiquitin ligase Siah1 in adrenal gland development and primary aldosteronism.
Thu Dec 07 00:00:00 UTC 2017
G-protein coupled receptor 15 mediates angiogenesis and cytoprotective function of thrombomodulin.
Scientific reports
Pan B,Wang X,Nishioka C,Honda G,Yokoyama A,Zeng L,Xu K,Ikezoe T
R960-25 was used in Immunocytochemistry to suggest that GPR15 plays an important role in mediating cytoprotective function as well as angiogenesis of thrombomodulin.
Thu Apr 06 00:00:00 UTC 2017
PI3K-C2α knockdown decreases autophagy and maturation of endocytic vesicles.
PloS one
Merrill NM,Schipper JL,Karnes JB,Kauffman AL,Martin KR,MacKeigan JP
R960-25 was used in Immunofluorescence to support a role for PI3K-C2α in the proper maturation of endosomes and connecting the endocytic and autophagic pathways.
Thu Oct 12 00:00:00 UTC 2017
PI3K-C2α knockdown decreases autophagy and maturation of endocytic vesicles.
PloS one
Merrill NM,Schipper JL,Karnes JB,Kauffman AL,Martin KR,MacKeigan JP
R960-25 was used in Immunofluorescence to support a role for PI3K-C2α in the proper maturation of endosomes and connecting the endocytic and autophagic pathways.
Thu Oct 12 00:00:00 UTC 2017
Epithelial Gpr116 regulates pulmonary alveolar homeostasis via Gq/11 signaling.
JCI insight
Brown K,Filuta A,Ludwig MG,Seuwen K,Jaros J,Vidal S,Arora K,Naren AP,Kandasamy K,Parthasarathi K,Offermanns S,Mason RJ,Miller WE,Whitsett JA,Bridges JP
R960-25 was used in Immunocytochemistry to identify GPR116 as a plausible target for modulating endogenous alveolar surfactant pools, to treat pulmonary diseases associated with surfactant dysfunction.
Fri Jun 02 00:00:00 UTC 2017
The lack of the organic cation transporter OCT1 at the plasma membrane of tumor cells precludes a positive response to sorafenib in patients with hepatocellular carcinoma.
Oncotarget
Geier A,Macias RI,Bettinger D,Weiss J,Bantel H,Jahn D,Al-Abdulla R,Marin JJ
R960-25 was used in Immunofluorescence to indicate the presence of OCT1 at the plasma membrane is related to better outcome of HCC patients treated with sorafenib.
Tue Feb 28 00:00:00 UTC 2017
Short-chain fatty acid receptors inhibit invasive phenotypes in breast cancer cells.
PloS one
Thirunavukkarasan M,Wang C,Rao A,Hind T,Teo YR,Siddiquee AA,Goghari MAI,Kumar AP,Herr DR
R960-25 was used in Immunocytochemistry to evaluate the effects of enforced overexpression of two known G protein-coupled receptors in two phenotypically distinct breast cancer cell lines.
Mon Nov 20 00:00:00 UTC 2017
Intracellular Proteolysis of Progranulin Generates Stable, Lysosomal Granulins that Are Haploinsufficient in Patients with Frontotemporal Dementia Caused by GRN Mutations.
eNeuro
Holler CJ,Taylor G,Deng Q,Kukar T
R960-25 was used in Western Blotting to report the identification and characterisation of antibodies that reliably detect several human GRNs by immunoblot and immunocytochemistry.
Thu May 03 00:00:00 UTC 2018
Neuropilin 1 sequestration by neuropathogenic mutant glycyl-tRNA synthetase is permissive to vascular homeostasis.
Scientific reports
Sleigh JN,Gómez-Martín A,Wei N,Bai G,Yang XL,Schiavo G
Wed Aug 23 00:00:00 UTC 2017
Interferon signaling in Peromyscus leucopus confers a potent and specific restriction to vector-borne flaviviruses.
PloS one
Izuogu AO,McNally KL,Harris SE,Youseff BH,Presloid JB,Burlak C,Munshi-South J,Best SM,Taylor RT
R960-25 was used in Immunoprecipitation to compare tick-borne flavirivirus infection between reservoir host cells and related susceptible species.
Mon Sep 18 00:00:00 UTC 2017
Genetically encoded photocross-linkers determine the biological binding site of exendin-4 peptide in the N-terminal domain of the intact human glucagon-like peptide-1 receptor (GLP-1R).
The Journal of biological chemistry
Koole C,Reynolds CA,Mobarec JC,Hick C,Sexton PM,Sakmar TP
R960-25 was used in Western Blotting to link the biological activity of the glucagon-like peptide-1 receptor with the molecular structure of an intact, full-length, and functional receptor-ligand complex.
Fri Apr 28 00:00:00 UTC 2017
Time-gated detection of protein-protein interactions with transcriptional readout.
eLife
Kim MW,Wang W,Sanchez MI,Coukos R,von Zastrow M,Ting AY
R960-25 was used in Immunofluorescence to create the assay SPARK, which has the potential to advance protein-protein interaction analysis and discovery.
Thu Nov 30 00:00:00 UTC 2017
DDB2 Is a Novel Regulator of Wnt Signaling in Colon Cancer.
Cancer research
Huang S,Fantini D,Merrill BJ,Bagchi S,Guzman G,Raychaudhuri P
R960-25 was used in Western Blot to identify DDB2 as both a partner and regulator of Wnt signalling, with an important role in suppressing colon cancer development.
Fri Dec 01 00:00:00 UTC 2017
UVB-induced nuclear translocation of TC-PTP by AKT/14-3-3σ axis inhibits keratinocyte survival and proliferation.
Oncotarget
Kim M,Morales LD,Baek M,Slaga TJ,DiGiovanni J,Kim DJ
R960-25 was used in Immunocytochemistry to report that TC45 is translocated by an AKT/14-3-3σ-mediated mechanism in response to UVB exposure, resulting in increased apoptosis and decreased keratinocyte proliferation.
Tue Oct 31 00:00:00 UTC 2017
The T-LAK Cell-originated Protein Kinase Signal Pathway Promotes Colorectal Cancer Metastasis.
EBioMedicine
Zykova TA,Zhu F,Wang L,Li H,Bai R,Lim DY,Yao K,Bode AM,Dong Z
R960-25 was used in Immunoprecipitation to investigate the role of T-LAK cell-originated protein kinase in the promotion of colorectal cancer metastasis.
Sat Apr 01 00:00:00 UTC 2017
RORα controls hepatic lipid homeostasis via negative regulation of PPARγ transcriptional network.
Nature communications
Kim K,Boo K,Yu YS,Oh SK,Kim H,Jeon Y,Bhin J,Hwang D,Kim KI,Lee JS,Im SS,Yoon SG,Kim IY,Seong JK,Lee H,Fang S,Baek SH
R960-25 was used in Western Blotting to show that the nuclear receptor RORα inhibits PPARγ-mediated transcriptional activity by interacting with HDAC3 and competing for the promoters of lipogenic genes.
Mon Jul 31 00:00:00 UTC 2017
ER residency of the ceramide phosphoethanolamine synthase SMSr relies on homotypic oligomerization mediated by its SAM domain.
Scientific reports
Cabukusta B,Kol M,Kneller L,Hilderink A,Bickert A,Mina JG,Korneev S,Holthuis JC
R96025 was used in immunocytochemistry, immunoprecipitation, and western blot to examine if SMSr-SAM is structurally and functionally related to the SAM domain of diacylglycerol kinase DGKd
Wed Jan 25 00:00:00 UTC 2017
Tracing neuronal circuits in transgenic animals by transneuronal control of transcription (TRACT).
eLife
Huang TH,Niesman P,Arasu D,Lee D,De La Cruz AL,Callejas A,Hong EJ,Lois C
R960-25 was used in Immunocytochemistry to demonstrate that the TRACT method can be used to investigate the connectivity of neuronal circuits in the brain.
Tue Dec 12 00:00:00 UTC 2017
The stem cell/cancer stem cell marker ALDH1A3 regulates the expression of the survival factor tissue transglutaminase, in mesenchymal glioma stem cells.
Oncotarget
Sullivan KE,Rojas K,Cerione RA,Nakano I,Wilson KF
R960-25 was used in Western Blotting to study a novel function of aldehyde dehydrogenase 1A3 in an aggressive glioma stem cell phenotype.
Tue Apr 04 00:00:00 UTC 2017
Inhibition of cholesterol biosynthesis through RNF145-dependent ubiquitination of SCAP.
eLife
Zhang L,Rajbhandari P,Priest C,Sandhu J,Wu X,Temel R,Castrillo A,de Aguiar Vallim TQ,Sallam T,Tontonoz P
R960-25 was used in Western Blotting to identify the E3 ligase RNF145 as an inhibitor of hepatic SREBP-2 processing, outlining an additional mechanism for LXR-SREBP crosstalk in lipid homeostasis.
Wed Oct 25 00:00:00 UTC 2017
Human cytomegalovirus-encoded viral cyclin-dependent kinase (v-CDK) UL97 phosphorylates and inactivates the retinoblastoma protein-related p107 and p130 proteins.
The Journal of biological chemistry
Iwahori S,Umaña AC,VanDeusen HR,Kalejta RF
R960-25 was used in Western Blotting to identify the retinoblastoma protein-related proteins, p107 and p130, as novel targets of human cytomegalovirus-encoded UL97.
Fri Apr 21 00:00:00 UTC 2017
The PINK1 p.I368N mutation affects protein stability and ubiquitin kinase activity.
Molecular neurodegeneration
Ando M,Fiesel FC,Hudec R,Caulfield TR,Ogaki K,Górka-Skoczylas P,Koziorowski D,Friedman A,Chen L,Dawson VL,Dawson TM,Bu G,Ross OA,Wszolek ZK,Springer W
R960-25 was used in Western Blotting to characterise the pathogenicity of the PINK1 p.I368N mutation on the clinical, genetic, structural and functional level in patients' fibroblasts and in cell-based, biochemical assays.
Mon Apr 24 00:00:00 UTC 2017
Structure of Fam20A reveals a pseudokinase featuring a unique disulfide pattern and inverted ATP-binding.
eLife
Cui J,Zhu Q,Zhang H,Cianfrocco MA,Leschziner AE,Dixon JE,Xiao J
R960-25 was used in Western Blotting to examine the structure of Fam20A, and how this relates to it's function, and disease-causing mutations.
Sat Apr 22 00:00:00 UTC 2017
Dissection of the interaction between the intrinsically disordered YAP protein and the transcription factor TEAD.
eLife
Mesrouze Y,Bokhovchuk F,Meyerhofer M,Fontana P,Zimmermann C,Martin T,Delaunay C,Erdmann D,Schmelzle T,Chène P
R960-25 was used in Western Blotting to provide quantitative understanding of the interactions taking place at the YAP:TEAD interface and give insights into the formation of the YAP:TEAD complex.
Fri Apr 21 00:00:00 UTC 2017
Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration.
Nature communications
Liu JA,Rao Y,Cheung MPL,Hui MN,Wu MH,Chan LK,Ng IO,Niu B,Cheah KSE,Sharma R,Hodgson L,Cheung M
R960-25 was used in Western Blotting to present a SOX9/SOX10-NEDD9/DLC1-RHOA regulatory axis to govern neural crest cell migratory polarisation.
Mon Oct 30 00:00:00 UTC 2017
Roles of the low density lipoprotein receptor and related receptors in inhibition of lipoprotein(a) internalization by proprotein convertase subtilisin/kexin type 9.
PloS one
Romagnuolo R,Scipione CA,Marcovina SM,Gemin M,Seidah NG,Boffa MB,Koschinsky ML
R960-25 was used in Western Blotting to support the idea PCSK9 inhibits Lp(a) clearance through the LDLR, but do not exclude other effects of PCSK9 such as on Lp(a) biosynthesis.
Mon Oct 09 00:00:00 UTC 2017
Angiotensin-converting enzyme Ance is cooperatively regulated by Mad and Pannier in Drosophila imaginal discs.
Scientific reports
Kim AR,Choi EB,Kim MY,Choi KW
R960-25 was used in Western Blotting to demonstrate that Drosophila Ance expression is regulated by a combination of Mad and Pannier in imaginal discs.
Fri Oct 13 00:00:00 UTC 2017
The Trypanosoma brucei dihydroxyacetonephosphate acyltransferase TbDAT is dispensable for normal growth but important for synthesis of ether glycerophospholipids.
PloS one
Zufferey R,Pirani K,Cheung-See-Kit M,Lee S,Williams TA,Chen DG,Hossain MF
R960-25 was used in Immunofluorescence to show that in Trypanosoma brucei procyclic forms, TbDAT but not TbGAT is the physiologically relevant initial acyltransferase.
Tue Oct 24 00:00:00 UTC 2017
Inhibitor repurposing reveals ALK, LTK, FGFR, RET and TRK kinases as the targets of AZD1480.
Oncotarget
Gudernova I,Balek L,Varecha M,Kucerova JF,Kunova Bosakova M,Fafilek B,Palusova V,Uldrijan S,Trantirek L,Krejci P
R960-25 was used in Western Blotting to reveal novel targets of the protein kinase inhibitor, AZD1480.
Tue Dec 12 00:00:00 UTC 2017
Specific Palmitoyltransferases Associate with and Activate the Epithelial Sodium Channel.
The Journal of biological chemistry
Mukherjee A,Wang Z,Kinlough CL,Poland PA,Marciszyn AL,Montalbetti N,Carattino MD,Butterworth MB,Kleyman TR,Hughey RP
R960-25 was used in Western Blotting to demonstrate that specific palmitoyltransferases have a role in regulating the epithelial sodium channel.
Fri Mar 10 00:00:00 UTC 2017
Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts.
Cell
Loh KH,Stawski PS,Draycott AS,Udeshi ND,Lehrman EK,Wilton DK,Svinkina T,Deerinck TJ,Ellisman MH,Stevens B,Carr SA,Ting AY
R96025 was used in immunocytochemistry and western blot to analyze the proteomic profile of synaptic clefts
Thu Aug 25 00:00:00 UTC 2016
Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia.
Nature genetics
Broix L,Jagline H,Ivanova E,Schmucker S,Drouot N,Clayton-Smith J,Pagnamenta AT,Metcalfe KA,Isidor B,Louvier UW,Poduri A,Taylor JC,Tilly P,Poirier K,Saillour Y,Lebrun N,Stemmelen T,Rudolf G,Muraca G,Saintpierre B,Elmorjani A,Moïse M,Weirauch NB,Guerrini R,Boland A,Olaso R,Masson C,Tripathy R,Keays D,Beldjord C,Nguyen L,Godin J,Kini U,Nischké P,Deleuze JF,Bahi-Buisson N,Sumara I,Hinckelmann MV,Chelly J
R96025 was used in immunoprecipitation and western blot to demonstrate that missense mutations in NEDD4L result in periventricular nodular heterotopia associated with toe syndactyly, cleft palate, and neurodevelopmental delay
Tue Nov 01 00:00:00 UTC 2016
Itch inhibits IL-17-mediated colon inflammation and tumorigenesis by ROR-γt ubiquitination.
Nature immunology
Kathania M,Khare P,Zeng M,Cantarel B,Zhang H,Ueno H,Venuprasad K
R960-25 was used in western blot to analyze inhibition of IL-17-mediated colon inflammation and tumorigenesis by ROR-gamma-t ubiquitination by Itch
Mon Aug 01 00:00:00 UTC 2016
Directing an artificial zinc finger protein to new targets by fusion to a non-DNA-binding domain.
Nucleic acids research
Lim WF,Burdach J,Funnell AP,Pearson RC,Quinlan KG,Crossley M
R960CUS was used in ChIP assay to show that FDs can influence and may be useful in directing AZF DNA-binding proteins to specific targets and provide insights into how natural transcription factors operate.
Wed Apr 20 00:00:00 UTC 2016
Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2.
Nature protocols
Hung V,Udeshi ND,Lam SS,Loh KH,Cox KJ,Pedram K,Carr SA,Ting AY
R960-25 was used in immunocytochemistry and western blot to study engineered peroxidase APEX2 and how it can spatially resolve proteomic mapping in livving cells
Tue Mar 01 00:00:00 UTC 2016
Higher-order oligomerization promotes localization of SPOP to liquid nuclear speckles.
The EMBO journal
Marzahn MR,Marada S,Lee J,Nourse A,Kenrick S,Zhao H,Ben-Nissan G,Kolaitis RM,Peters JL,Pounds S,Errington WJ,Privé GG,Taylor JP,Sharon M,Schuck P,Ogden SK,Mittag T
R960-25 was used in Western Blotting to show that BTB-mediated speckle-type POZ protein dimers form linear oligomers via BACK domain dimerisation, and determine the concentration-dependent populations of the resulting oligomeric species.
Wed Jun 15 00:00:00 UTC 2016
ANKS4B Is Essential for Intermicrovillar Adhesion Complex Formation.
Developmental cell
Crawley SW,Weck ML,Grega-Larson NE,Shifrin DA,Tyska MJ
R960-25 was used in western blot to investigate the requirement of ANKS4B for intermicrovillar adhesion complex formation
Mon Jan 25 00:00:00 UTC 2016
Acidosis Acts through HSP90 in a PHD/VHL-Independent Manner to Promote HIF Function and Stem Cell Maintenance in Glioma.
Cancer research
Filatova A,Seidel S,Böğürcü N,Gräf S,Garvalov BK,Acker T
R960-25 was used in Western Blotting to highlight tumour acidifications role within the hypoxic niche in the regulation of hypoxia-inducible factors and CSC function through HSP90.
Sat Oct 01 00:00:00 UTC 2016
Functional screen identifies kinases driving prostate cancer visceral and bone metastasis.
Proceedings of the National Academy of Sciences of the United States of America
Faltermeier CM,Drake JM,Clark PM,Smith BA,Zong Y,Volpe C,Mathis C,Morrissey C,Castor B,Huang J,Witte ON
R960-25 was used in western blot to elucidate a screening that identifies kinases involved in prostate cancer visceral and bone metastasis
Tue Jan 12 00:00:00 UTC 2016
Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features.
Proceedings of the National Academy of Sciences of the United States of America
Ouyang Q,Nakayama T,Baytas O,Davidson SM,Yang C,Schmidt M,Lizarraga SB,Mishra S,Ei-Quessny M,Niaz S,Gul Butt M,Imran Murtaza S,Javed A,Chaudhry HR,Vaughan DJ,Hill RS,Partlow JN,Yoo SY,Lam AT,Nasir R,Al-Saffar M,Barkovich AJ,Schwede M,Nagpal S,Rajab A,DeBerardinis RJ,Housman DE,Mochida GH,Morrow EM
R960-25 was used in Immunocytochemistry-immunoflourescence to reveal a role for the glutamate pyruvate transaminase 2 enzyme in mitochondrial metabolism with relevance to developmental and potentially neurodegenerative mechanisms.
Tue Sep 20 00:00:00 UTC 2016
Overexpression screens identify conserved dosage chromosome instability genes in yeast and human cancer.
Proceedings of the National Academy of Sciences of the United States of America
Duffy S,Fam HK,Wang YK,Styles EB,Kim JH,Ang JS,Singh T,Larionov V,Shah SP,Andrews B,Boerkoel CF,Hieter P
R960-25 was used in Western Blotting to catalogue a list of candidate genes thought to cause chromosome instability (CIN) when overexpressed in human cancer.
Tue Sep 06 00:00:00 UTC 2016
CCDC115 Deficiency Causes a Disorder of Golgi Homeostasis with Abnormal Protein Glycosylation.
American journal of human genetics
Jansen JC,Cirak S,van Scherpenzeel M,Timal S,Reunert J,Rust S,Pérez B,Vicogne D,Krawitz P,Wada Y,Ashikov A,Pérez-Cerdá C,Medrano C,Arnoldy A,Hoischen A,Huijben K,Steenbergen G,Quelhas D,Diogo L,Rymen D,Jaeken J,Guffon N,Cheillan D,van den Heuvel LP,Maeda Y,Kaiser O,Schara U,Gerner P,van den Boogert MA,Holleboom AG,Nassogne MC,Sokal E,Salomon J,van den Bogaart G,Drenth JP,Huynen MA,Veltman JA,Wevers RA,Morava E,Matthijs G,Foulquier F,Marquardt T,Lefeber DJ
R960-25 was used in immunocytochemistry to study the cause of a golgi homeostasis disorder with abnormal protein glycosylation due to CCDC115 deficiency
Thu Feb 04 00:00:00 UTC 2016
Mutations in Three Genes Encoding Proteins Involved in Hair Shaft Formation Cause Uncombable Hair Syndrome.
American journal of human genetics
Ü Basmanav FB,Cau L,Tafazzoli A,Méchin MC,Wolf S,Romano MT,Valentin F,Wiegmann H,Huchenq A,Kandil R,Garcia Bartels N,Kilic A,George S,Ralser DJ,Bergner S,Ferguson DJP,Oprisoreanu AM,Wehner M,Thiele H,Altmüller J,Nürnberg P,Swan D,Houniet D,Büchner A,Weibel L,Wagner N,Grimalt R,Bygum A,Serre G,Blume-Peytavi U,Sprecher E,Schoch S,Oji V,Hamm H,Farrant P,Simon M,Betz RC
R96025 was used in immunohistochemistry to find mutations in peptidylarginine deiminase 3, transglutaminase 3, and trichohyalin in children with uncombable hair syndrome
Thu Dec 01 00:00:00 UTC 2016
TMEM199 Deficiency Is a Disorder of Golgi Homeostasis Characterized by Elevated Aminotransferases, Alkaline Phosphatase, and Cholesterol and Abnormal Glycosylation.
American journal of human genetics
Jansen JC,Timal S,van Scherpenzeel M,Michelakakis H,Vicogne D,Ashikov A,Moraitou M,Hoischen A,Huijben K,Steenbergen G,van den Boogert MA,Porta F,Calvo PL,Mavrikou M,Cenacchi G,van den Bogaart G,Salomon J,Holleboom AG,Rodenburg RJ,Drenth JP,Huynen MA,Wevers RA,Morava E,Foulquier F,Veltman JA,Lefeber DJ
Thu Feb 04 00:00:00 UTC 2016
Biallelic TBCD Mutations Cause Early-Onset Neurodegenerative Encephalopathy.
American journal of human genetics
Miyake N,Fukai R,Ohba C,Chihara T,Miura M,Shimizu H,Kakita A,Imagawa E,Shiina M,Ogata K,Okuno-Yuguchi J,Fueki N,Ogiso Y,Suzumura H,Watabe Y,Imataka G,Leong HY,Fattal-Valevski A,Kramer U,Miyatake S,Kato M,Okamoto N,Sato Y,Mitsuhashi S,Nishino I,Kaneko N,Nishiyama A,Tamura T,Mizuguchi T,Nakashima M,Tanaka F,Saitsu H,Matsumoto N
R960-25 was used in Western Blotting to study how defects of microtubule formation caused by TBCD mutations may underlie the pathomechanism of this neurodegenerative encephalopathy.
Thu Oct 06 00:00:00 UTC 2016
Role of Angiomotin-like 2 mono-ubiquitination on YAP inhibition.
EMBO reports
Kim M,Kim M,Park SJ,Lee C,Lim DS
R960-25 was used in immunoprecipitation and western blot to study the activation mechanisms of core Hippo pathway components
Fri Jan 01 00:00:00 UTC 2016
Ceramide limits phosphatidylinositol-3-kinase C2β-controlled cell motility in ovarian cancer: potential of ceramide as a metastasis-suppressor lipid.
Oncogene
Kitatani K,Usui T,Sriraman SK,Toyoshima M,Ishibashi M,Shigeta S,Nagase S,Sakamoto M,Ogiso H,Okazaki T,Hannun YA,Torchilin VP,Yaegashi N
R960-25 was used in Immunocytochemistry-immunoflourescence and Western blot to examine the role of ceramide in ovarian cancer metastasis.
Sun May 01 00:00:00 UTC 2016
Ceramide limits phosphatidylinositol-3-kinase C2β-controlled cell motility in ovarian cancer: potential of ceramide as a metastasis-suppressor lipid.
Oncogene
Kitatani K,Usui T,Sriraman SK,Toyoshima M,Ishibashi M,Shigeta S,Nagase S,Sakamoto M,Ogiso H,Okazaki T,Hannun YA,Torchilin VP,Yaegashi N
R960-25 was used in Immunocytochemistry-immunoflourescence and Western blot to examine the role of ceramide in ovarian cancer metastasis.
Sun May 01 00:00:00 UTC 2016
XRN1 Is a Species-Specific Virus Restriction Factor in Yeasts.
PLoS pathogens
Rowley PA,Ho B,Bushong S,Johnson A,Sawyer SL
R960-25 was used in Western Blotting to examine the species-specific relationship between Xrn1p and the "L-A" totivirus of different Saccharomyces yeast species.
Sat Oct 01 00:00:00 UTC 2016
XRN1 Is a Species-Specific Virus Restriction Factor in Yeasts.
PLoS pathogens
Rowley PA,Ho B,Bushong S,Johnson A,Sawyer SL
R960-25 was used in Western Blotting to examine the species-specific relationship between Xrn1p and the "L-A" totivirus of different Saccharomyces yeast species.
Sat Oct 01 00:00:00 UTC 2016
Cytoadhesion to gC1qR through Plasmodium falciparum Erythrocyte Membrane Protein 1 in Severe Malaria.
PLoS pathogens
Magallón-Tejada A,Machevo S,Cisteró P,Lavstsen T,Aide P,Rubio M,Jiménez A,Turner L,Valmaseda A,Gupta H,De Las Salas B,Mandomando I,Wang CW,Petersen JE,Muñoz J,Gascón J,Macete E,Alonso PL,Chitnis CE,Bassat Q,Mayor A
R960-25 was used in Western Blotting to support a key role for gC1qR in malaria-associated endovascular pathogenesis.
Tue Nov 01 00:00:00 UTC 2016
cGMP-Dependent Protein Kinase Encoded by foraging Regulates Motor Axon Guidance in Drosophila by Suppressing Lola Function.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Peng Q,Wang Y,Li M,Yuan D,Xu M,Li C,Gong Z,Jiao R,Liu L
R960-25 was used in Immunofluorescence to highlight a new function of PKG in axon guidance by suppressing a transcription factor.
Wed Apr 20 00:00:00 UTC 2016
APOBEC3G and APOBEC3F Act in Concert To Extinguish HIV-1 Replication.
Journal of virology
Krisko JF,Begum N,Baker CE,Foster JL,Garcia JV
R960-25 was used in western blot to assess extinguishing of HIV-1 replication by cooperation of APOBEC3F and APOBEC3G
Sun May 01 00:00:00 UTC 2016
Protein-Protein Interactions Suggest Novel Activities of Human Cytomegalovirus Tegument Protein pUL103.
Journal of virology
Ortiz DA,Glassbrook JE,Pellett PE
R960-25 was used in immunocytochemistry and immunoprecipitation to study novel activities of human cytomegalovirus tegument protein pUL103 by study of protein-protein interactions
Thu Sep 01 00:00:00 UTC 2016
ZEB1 induces EPB41L5 in the cancer mesenchymal program that drives ARF6-based invasion, metastasis and drug resistance.
Oncogenesis
Hashimoto A,Hashimoto S,Sugino H,Yoshikawa A,Onodera Y,Handa H,Oikawa T,Sabe H
R960-25 was used in Western Blotting to elucidate the involvement of EPB41L5 in the ARF6-based pathway, following induction by ZEB1.
Mon Sep 12 00:00:00 UTC 2016
Mastermind-Like 3 Controls Proliferation and Differentiation in Neuroblastoma.
Molecular cancer research : MCR
Heynen GJ,Nevedomskaya E,Palit S,Jagalur Basheer N,Lieftink C,Schlicker A,Zwart W,Bernards R,Bajpe PK
R960-25 was used in western blot to study the role of MAML3 in proliferation and retinoic acid-mediated differentiation
Sun May 01 00:00:00 UTC 2016
TNFR-Associated Factors 2 and 5 Differentially Regulate the Instructive IL-6 Receptor Signaling Required for Th17 Development.
Journal of immunology (Baltimore, Md. : 1950)
Nagashima H,Okuyama Y,Hayashi T,Ishii N,So T
R960-25 was used in immunoprecipitation and western blot to characterize differential regulation of the instructive IL-6 receptor signaling required for Th17 development by TNFR-associated factors 2 and 5
Sun May 15 00:00:00 UTC 2016
SEPT8 modulates β-amyloidogenic processing of APP by affecting the sorting and accumulation of BACE1.
Journal of cell science
Kurkinen KM,Marttinen M,Turner L,Natunen T,Mäkinen P,Haapalinna F,Sarajärvi T,Gabbouj S,Kurki M,Paananen J,Koivisto AM,Rauramaa T,Leinonen V,Tanila H,Soininen H,Lucas FR,Haapasalo A,Hiltunen M
R960-25 was used in immunocytochemistry to research the modulation of beta-amyloidogenic processing of APP by affecting the sorting and accumulation of BACE1 by SEPT8
Wed Jun 01 00:00:00 UTC 2016
Sorting nexin 9 negatively regulates invadopodia formation and function in cancer cells.
Journal of cell science
Bendris N,Stearns CJ,Reis CR,Rodriguez-Canales J,Liu H,Witkiewicz AW,Schmid SL
R960-25 was used in Western Blotting to identify sorting nexin 9 (SNX9) expression as a direct metastatic potential biomarker for human cancers.
Fri Jul 15 00:00:00 UTC 2016
Neuronal deficiency of ARV1 causes an autosomal recessive epileptic encephalopathy.
Human molecular genetics
Palmer EE,Jarrett KE,Sachdev RK,Al Zahrani F,Hashem MO,Ibrahim N,Sampaio H,Kandula T,Macintosh R,Gupta R,Conlon DM,Billheimer JT,Rader DJ,Funato K,Walkey CJ,Lee CS,Loo C,Brammah S,Elakis G,Zhu Y,Buckley M,Kirk EP,Bye A,Alkuraya FS,Roscioli T,Lagor WR
R960-25 was used in Western Blotting to support ARV1 deficiency as a cause of autosomal recessive epileptic encephalopathy.
Fri Jul 15 00:00:00 UTC 2016
Zfp703 Is a Wnt/β-Catenin Feedback Suppressor Targeting the β-Catenin/Tcf1 Complex.
Molecular and cellular biology
Kumar A,Chalamalasetty RB,Kennedy MW,Thomas S,Inala SN,Garriock RJ,Yamaguchi TP
R960-25 was used in Western Blotting to elucidate the role of Zfp703 as a novel feedback suppressor of Wnt/Β-catenin signalling through its inhibition of Β-catenin/Tcf1 complex formation.
Wed Jun 15 00:00:00 UTC 2016
Expanding the Phenotype Associated with NAA10-Related N-Terminal Acetylation Deficiency.
Human mutation
Saunier C,Støve SI,Popp B,Gérard B,Blenski M,AhMew N,de Bie C,Goldenberg P,Isidor B,Keren B,Leheup B,Lampert L,Mignot C,Tezcan K,Mancini GM,Nava C,Wasserstein M,Bruel AL,Thevenon J,Masurel A,Duffourd Y,Kuentz P,Huet F,Rivière JB,van Slegtenhorst M,Faivre L,Piton A,Reis A,Arnesen T,Thauvin-Robinet C,Zweier C
R960-25 was used in Western Blotting to characterise the phenotype associated with NAA10-related N-terminal acetylation deficiency.
Mon Aug 01 00:00:00 UTC 2016
Challenges in Targeting a Basic Helix-Loop-Helix Transcription Factor with Hydrocarbon-Stapled Peptides.
ACS chemical biology
Edwards AL,Meijer DH,Guerra RM,Molenaar RJ,Alberta JA,Bernal F,Bird GH,Stiles CD,Walensky LD
R960-25 was used in Western Blotting to highlight potential pitfalls in peptide-based targeting of bHLH transcription factors given the liabilities of their positively charged amino acid sequences and multifactorial binding determinants.
Fri Nov 18 00:00:00 UTC 2016
The SLE variant Ala71Thr of BLK severely decreases protein abundance and binding to BANK1 through impairment of the SH3 domain function.
Genes and immunity
Díaz-Barreiro A,Bernal-Quirós M,Georg I,Marañón C,Alarcón-Riquelme ME,Castillejo-López C
R960-25 was used in western blot to study the impact of different B-lymphocyte kinase variants
Tue Mar 01 00:00:00 UTC 2016
The exceptionally high reactivity of Cys 621 is critical for electrophilic activation of the sensory nerve ion channel TRPA1.
The Journal of general physiology
Bahia PK,Parks TA,Stanford KR,Mitchell DA,Varma S,Stevens SM,Taylor-Clark TE
R960-25 was used in immunocytochemistry, immunoprecipitation, and western blot to determine the critical component for electrophilic activation of the sensory nerve ion channel TRPA1 by high reactivity of Cys 621
Wed Jun 01 00:00:00 UTC 2016
Analysis of the Fam181 gene family during mouse development reveals distinct strain-specific expression patterns, suggesting a role in nervous system development and function.
Gene
Marks M,Pennimpede T,Lange L,Grote P,Herrmann BG,Wittler L
R960-25 was used in immunocytochemistry and western blot to investigate the role and localization of Fam181b and Fam181a.
Sun Jan 10 00:00:00 UTC 2016
Actinin-1 binds to the C-terminus of A2B adenosine receptor (A2BAR) and enhances A2BAR cell-surface expression.
The Biochemical journal
Sun Y,Hu W,Yu X,Liu Z,Tarran R,Ravid K,Huang P
R960-25 was used in western blot to elucidate enhancement of A2BAR cell-surface expression by actinin-1 binding to the C-terminus of A2B adenosine receptor (A2BAR)
Fri Jul 15 00:00:00 UTC 2016
Mouse Apolipoprotein L9 is a phosphatidylethanolamine-binding protein.
Biochemical and biophysical research communications
Arvind TA,Rangarajan PN
R96025 was used in immunocytochemistry to suggest that ApoL9 is a phosphatidylethanolamine-binding protein
Fri Oct 28 00:00:00 UTC 2016
Branched Fatty Acid Esters of Hydroxy Fatty Acids Are Preferred Substrates of the MODY8 Protein Carboxyl Ester Lipase.
Biochemistry
Kolar MJ,Kamat SS,Parsons WH,Homan EA,Maher T,Peroni OD,Syed I,Fjeld K,Molven A,Kahn BB,Cravatt BF,Saghatelian A
R960-25 was used in Western Blotting to suggest that carboxyl ester lipase might break down dietary fatty acid esters of hydroxy fatty acids.
Tue Aug 23 00:00:00 UTC 2016
Effects of transgenic expression of botulinum toxins in Drosophila.
Journal of neurogenetics
Backhaus P,Langenhan T,Neuser K
R960-25 was used in western blot to study botulinum toxins in drosophila and the effects of transgenic expression
Tue Mar 01 00:00:00 UTC 2016
Identification of the novel activity-driven interaction between synaptotagmin 1 and presenilin 1 links calcium, synapse, and amyloid beta.
BMC biology
Kuzuya A,Zoltowska KM,Post KL,Arimon M,Li X,Svirsky S,Maesako M,Muzikansky A,Gautam V,Kovacs D,Hyman BT,Berezovska O
R960-25 was used in western blot to study the activity-driven interaction between presenilin 1 and synaptotagmin 1 and links to calcium, amyloid beta, and synapse
Thu Mar 31 00:00:00 UTC 2016
Identification of NEK3 Kinase Threonine 165 as a Novel Regulatory Phosphorylation Site That Modulates Focal Adhesion Remodeling Necessary for Breast Cancer Cell Migration.
The Journal of biological chemistry
Harrington KM,Clevenger CV
R960-25 was used in Immunocytochemistry to identify a novel prolactin-inducible, regulatory phosphorylation site within the activation segment of NEK3.
Fri Oct 07 00:00:00 UTC 2016
PINK1 deficiency enhances autophagy and mitophagy induction.
Molecular & cellular oncology
Gómez-Sánchez R,Yakhine-Diop SM,Bravo-San Pedro JM,Pizarro-Estrella E,Rodríguez-Arribas M,Climent V,Martin-Cano FE,González-Soltero ME,Tandon A,Fuentes JM,González-Polo RA
R960-25 was used in immunocytochemistry to study enhanced mitophagy and autophagy induction by PINK1 deficiency
Tue Mar 01 00:00:00 UTC 2016
Ectodomain Shedding of Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE-1) Is Induced by Vascular Endothelial Growth Factor A (VEGF-A).
The Journal of biological chemistry
Nishida-Fukuda H,Araki R,Shudou M,Okazaki H,Tomono Y,Nakayama H,Fukuda S,Sakaue T,Shirakata Y,Sayama K,Hashimoto K,Detmar M,Higashiyama S,Hirakawa S
R960-25 was used in Immunoprecipitation to indicate that the ectodomain shedding of lymphatic vessel endothelial hyaluronan receptor 1 might be involved in promoting pathological lymphangiogenesis.
Fri May 13 00:00:00 UTC 2016
Ectodomain Shedding of Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE-1) Is Induced by Vascular Endothelial Growth Factor A (VEGF-A).
The Journal of biological chemistry
Nishida-Fukuda H,Araki R,Shudou M,Okazaki H,Tomono Y,Nakayama H,Fukuda S,Sakaue T,Shirakata Y,Sayama K,Hashimoto K,Detmar M,Higashiyama S,Hirakawa S
R960-25 was used in Immunoprecipitation to indicate that the ectodomain shedding of lymphatic vessel endothelial hyaluronan receptor 1 might be involved in promoting pathological lymphangiogenesis.
Fri May 13 00:00:00 UTC 2016
Diverse mechanisms of metaeffector activity in an intracellular bacterial pathogen, Legionella pneumophila.
Molecular systems biology
Urbanus ML,Quaile AT,Stogios PJ,Morar M,Rao C,Di Leo R,Evdokimova E,Lam M,Oatway C,Cuff ME,Osipiuk J,Michalska K,Nocek BP,Taipale M,Savchenko A,Ensminger AW
R96025 was used in western blot to determine the extent of Legionella pneumophila maintenance of translocated substrates through a systematic analysis of effector-effector functional interaction
Fri Dec 16 00:00:00 UTC 2016
The cancer-testis antigens SPANX-A/C/D and CTAG2 promote breast cancer invasion.
Oncotarget
Maine EA,Westcott JM,Prechtl AM,Dang TT,Whitehurst AW,Pearson GW
Tue Mar 22 00:00:00 UTC 2016
Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes.
The Journal of biological chemistry
Chen S,Lee B,Lee AY,Modzelewski AJ,He L
R960-25 was used in immunohistochemistry to study CRISPR ribonucleoprotein elctroporation of zygotes by highly efficient mouse genome editing
Fri Jul 08 00:00:00 UTC 2016
A Small-Molecule Anti-secretagogue of PCSK9 Targets the 80S Ribosome to Inhibit PCSK9 Protein Translation.
Cell chemical biology
Petersen DN,Hawkins J,Ruangsiriluk W,Stevens KA,Maguire BA,O'Connell TN,Rocke BN,Boehm M,Ruggeri RB,Rolph T,Hepworth D,Loria PM,Carpino PA
R960-25 was used in Western Blot to identify and demonstrate the mode of action of (R)-N-(isoquinolin-1-yl)-3-(4-methoxyphenyl)-N-(piperidin-3-yl)propanamide (R-IMPP), revealing its selective binding to human ribosomes and its role in transcript-dependent inhibition of PCSK9 translation, offering a novel pathway for drug development targeting eukaryotic translation to modulate protein activity.
Thu Nov 17 00:00:00 UTC 2016
The F-box Protein Rcy1 Is Involved in the Degradation of Histone H3 Variant Cse4 and Genome Maintenance.
The Journal of biological chemistry
Cheng H,Bao X,Rao H
R960-25 was used in Western Blotting to identify the involvement of Rcy1 in chromosome regulation and another regulatory pathway controlling Cse4 level and activity.
Fri May 06 00:00:00 UTC 2016
Repeat-Associated Fission Yeast-Like Regional Centromeres in the Ascomycetous Budding Yeast Candida tropicalis.
PLoS genetics
Chatterjee G,Sankaranarayanan SR,Guin K,Thattikota Y,Padmanabhan S,Siddharthan R,Sanyal K
R960-25 was used in Chromatin immunoprecipitation to demonstrate, for the first time, fission yeast-like repeat-associated centromeres in an ascomycetous budding yeast.
Mon Feb 01 00:00:00 UTC 2016
Molecular Determinants of GS-9620-Dependent TLR7 Activation.
PloS one
Rebbapragada I,Birkus G,Perry J,Xing W,Kwon H,Pflanz S
R960-25 was used in western blot to characterize GS-9620-dependent TLR7 activation and the associated molecular determinants
Tue Jul 26 00:00:00 UTC 2016
Longitudinal assessment of neuronal 3D genomes in mouse prefrontal cortex.
Nature communications
Mitchell AC,Javidfar B,Bicks LK,Neve R,Garbett K,Lander SS,Mirnics K,Morishita H,Wood MA,Jiang Y,Gaisler-Salomon I,Akbarian S
R960-25 was used in Chromatin immunoprecipitation to introduce 'NeuroDam' to assess epigenome status retrospectively and show an example in mice with NMDA receptor blockade.
Tue Sep 06 00:00:00 UTC 2016
The Skp1 Homologs SKR-1/2 Are Required for the Caenorhabditis elegans SKN-1 Antioxidant/Detoxification Response Independently of p38 MAPK.
PLoS genetics
Wu CW,Deonarine A,Przybysz A,Strange K,Choe KP
R960-25 was used in Western Blotting to identify a novel p38 MAPK independent signaling mechanism that activates SKN-1 via SKR-1/2 and involves WDR-23 in C. elegans.
Sat Oct 01 00:00:00 UTC 2016
Nuclear bodies reorganize during myogenesis in vitro and are differentially disrupted by expression of FSHD-associated DUX4.
Skeletal muscle
Homma S,Beermann ML,Yu B,Boyce FM,Miller JB
R960-25 was used in Immunocytochemistry-immunoflourescence to show that nucleoli, PML bodies, and SC35 speckles reorganise during human myotube formation in vitro.
Thu Dec 01 00:00:00 UTC 2016
RhoA Proteolysis Regulates the Actin Cytoskeleton in Response to Oxidative Stress.
PloS one
Girouard MP,Pool M,Alchini R,Rambaldi I,Fournier AE
R960-25 was used in Western Blotting to study the effect of RhoA-NTF upregulation on the Actin cytoskeleton in response to oxidative stress.
Fri Jun 30 00:00:00 UTC 2017
Pathogenic Mutations in the Valosin-containing Protein/p97(VCP) N-domain Inhibit the SUMOylation of VCP and Lead to Impaired Stress Response.
The Journal of biological chemistry
Wang T,Xu W,Qin M,Yang Y,Bao P,Shen F,Zhang Z,Xu J
R960-25 was used in immunoprecipitation and western blot to assess inhibition of the SUMOylation of VCP that leads to impaired stress response by pathogenic mutations in the valosin-containing protein/p97(VCP) N-domain
Fri Jul 01 00:00:00 UTC 2016
The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development.
Nature communications
Rautengarten C,Ebert B,Liu L,Stonebloom S,Smith-Moritz AM,Pauly M,Orellana A,Scheller HV,Heazlewood JL
R960-25 was used in western blot to determine the requirement for plant development by arabidopsis golgi-localized GDP-L-fucose transporter
Wed Jul 06 00:00:00 UTC 2016
The RNA-binding protein vigilin regulates VLDL secretion through modulation of Apob mRNA translation.
Nature communications
Mobin MB,Gerstberger S,Teupser D,Campana B,Charisse K,Heim MH,Manoharan M,Tuschl T,Stoffel M
R960-25 was used in Western Blotting to uncover a role for vigilin as a key regulator of hepatic Apob translation and demonstrate the therapeutic potential of inhibiting vigilin for cardiovascular diseases.
Mon Sep 26 00:00:00 UTC 2016
Bolaamphiphile-based nanocomplex delivery of phosphorothioate gapmer antisense oligonucleotides as a treatment for Clostridium difficile.
International journal of nanomedicine
Hegarty JP,Krzeminski J,Sharma AK,Guzman-Villanueva D,Weissig V,Stewart DB
R960-25 was used in Western Blotting to describe the novel application of cationic bolasomes to deliver antisense oligonucleotides into bacteria and report the first successful in vitro antisense treatment to inhibit the growth of Clostridium difficile.
Mon Mar 06 00:00:00 UTC 2017
An Unbiased Mass Spectrometry Approach Identifies Glypican-3 as an Interactor of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR) in Hepatocellular Carcinoma Cells.
The Journal of biological chemistry
Ly K,Essalmani R,Desjardins R,Seidah NG,Day R
R960-25 was used in Immunoprecipitation to identify proprotein convertase subtilisin/kexin type 9-interacting proteins in the HepG2 cells' secretome.
Fri Nov 18 00:00:00 UTC 2016
Developing therapeutically more efficient Neurturin variants for treatment of Parkinson's disease.
Neurobiology of disease
Runeberg-Roos P,Piccinini E,Penttinen AM,Mätlik K,Heikkinen H,Kuure S,Bespalov MM,Peränen J,Garea-Rodríguez E,Fuchs E,Airavaara M,Kalkkinen N,Penn R,Saarma M
R960-25 was used in Western Blotting and Immunocytochemistry to develop biologically active neurturin (NRTN) variants with reduced binding to heparin and improved intracranial diffusion for treatment of Parkinson's disease.
Thu Dec 01 00:00:00 UTC 2016
Developing therapeutically more efficient Neurturin variants for treatment of Parkinson's disease.
Neurobiology of disease
Runeberg-Roos P,Piccinini E,Penttinen AM,Mätlik K,Heikkinen H,Kuure S,Bespalov MM,Peränen J,Garea-Rodríguez E,Fuchs E,Airavaara M,Kalkkinen N,Penn R,Saarma M
R960-25 was used in Western Blotting and Immunocytochemistry to develop biologically active neurturin (NRTN) variants with reduced binding to heparin and improved intracranial diffusion for treatment of Parkinson's disease.
Thu Dec 01 00:00:00 UTC 2016
Premature polyadenylation of MAGI3 produces a dominantly-acting oncogene in human breast cancer.
eLife
Ni TK,Kuperwasser C
R960-25 was used in western blot to study the production of a dominantly-acting oncogene in human breast cancer due to premature polyadenylation of MAGI3
Fri May 20 00:00:00 UTC 2016
Identification of the BRD1 interaction network and its impact on mental disorder risk.
Genome medicine
Fryland T,Christensen JH,Pallesen J,Mattheisen M,Palmfeldt J,Bak M,Grove J,Demontis D,Blechingberg J,Ooi HS,Nyegaard M,Hauberg ME,Tommerup N,Gregersen N,Mors O,Corydon TJ,Nielsen AL,Børglum AD
R960-25 was used in Western Blotting to indicate that BRD1 acts as a regulatory hub in a comprehensive schizophrenia risk network.
Tue May 03 00:00:00 UTC 2016
Cholesterol-mediated Degradation of 7-Dehydrocholesterol Reductase Switches the Balance from Cholesterol to Vitamin D Synthesis.
The Journal of biological chemistry
Prabhu AV,Luu W,Sharpe LJ,Brown AJ
R960-25 was used in western blot to analyze the switch of balance from cholesterol to vitamin D synthesis by cholesterol-mediated degradation of 7-dehydrocholesterol reductase
Fri Apr 15 00:00:00 UTC 2016
Seizure protein 6 and its homolog seizure 6-like protein are physiological substrates of BACE1 in neurons.
Molecular neurodegeneration
Pigoni M,Wanngren J,Kuhn PH,Munro KM,Gunnersen JM,Takeshima H,Feederle R,Voytyuk I,De Strooper B,Levasseur MD,Hrupka BJ,Müller SA,Lichtenthaler SF
R960-25 was used in Inhibition experiments to identify seizure protein 6 and its ligand as physiological substrates of BACE1 (beta-site APP cleaving enzyme 1) in the murine brain.
Wed Oct 05 00:00:00 UTC 2016
E2F1 interactions with hHR23A inhibit its degradation and promote DNA repair.
Oncotarget
Singh RK,Dagnino L
R960-25 was used in Western Blot to study the relationship between E2F1 and hHR23 proteins vis-à-vis nucleotide excision repair.
Tue May 03 00:00:00 UTC 2016
DEEPN as an Approach for Batch Processing of Yeast 2-Hybrid Interactions.
Cell reports
Pashkova N,Peterson TA,Krishnamani V,Breheny P,Stamnes M,Piper RC
R960-25 was used in Western Blotting to demonstrate the specificity of dynamic enrichment for evaluation of protein networks.
Tue Sep 27 00:00:00 UTC 2016
Cloning of Glycerophosphocholine Acyltransferase (GPCAT) from Fungi and Plants: A NOVEL ENZYME IN PHOSPHATIDYLCHOLINE SYNTHESIS.
The Journal of biological chemistry
Głąb B,Beganovic M,Anaokar S,Hao MS,Rasmusson AG,Patton-Vogt J,Banaś A,Stymne S,Lager I
R960-25 was used in Western Blotting to identify and clone the yeast gene encoding glycerophosphocholine acyltransferase, a novel enzyme in phosphatidylcholine synthesis.
Fri Nov 25 00:00:00 UTC 2016
EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter.
Cell reports
Vais H,Mallilankaraman K,Mak DD,Hoff H,Payne R,Tanis JE,Foskett JK
R960-25 was used in western blot to elucidate the role of EMRE as a matrix Ca(2+) sensor that controls gatekeeping of the mitochondrial Ca(2+) uniporter
Tue Jan 26 00:00:00 UTC 2016
Internal epitope tagging informed by relative lack of sequence conservation.
Scientific reports
Burg L,Zhang K,Bonawitz T,Grajevskaja V,Bellipanni G,Waring R,Balciunas D
R96025 was used in immunohistochemistry to discuss epitope tagging of proteins
Mon Nov 28 00:00:00 UTC 2016
Aurora A drives early signalling and vesicle dynamics during T-cell activation.
Nature communications
Blas-Rus N,Bustos-Morán E,Pérez de Castro I,de Cárcer G,Borroto A,Camafeita E,Jorge I,Vázquez J,Alarcón B,Malumbres M,Martín-Cófreces NB,Sánchez-Madrid F
R960-25 was used in Immunoprecipitation to provide a mechanistic explanation as to how Aurora A kinase controls the activation of T lymphocytes during immunological synapse formation.
Tue Apr 19 00:00:00 UTC 2016
Aurora A drives early signalling and vesicle dynamics during T-cell activation.
Nature communications
Blas-Rus N,Bustos-Morán E,Pérez de Castro I,de Cárcer G,Borroto A,Camafeita E,Jorge I,Vázquez J,Alarcón B,Malumbres M,Martín-Cófreces NB,Sánchez-Madrid F
R960-25 was used in Immunoprecipitation to provide a mechanistic explanation as to how Aurora A kinase controls the activation of T lymphocytes during immunological synapse formation.
Tue Apr 19 00:00:00 UTC 2016
Targeting the ERK pathway for the treatment of Cushing's disease.
Oncotarget
Zhang D,Bergsneider M,Wang MB,Heaney AP
R960-25 was used in Western Blotting to identify the therapeutic potential of inhibitors of the Ras/Raf/MEK/ERK pathway for the treatment of Cushing's disease.
Tue Oct 25 00:00:00 UTC 2016
Smc5/6 Mediated Sumoylation of the Sgs1-Top3-Rmi1 Complex Promotes Removal of Recombination Intermediates.
Cell reports
Bonner JN,Choi K,Xue X,Torres NP,Szakal B,Wei L,Wan B,Arter M,Matos J,Sung P,Brown GW,Branzei D,Zhao X
R960-25 was used in Immunoprecipitation to study the roles of sumoylation and Smc5/6 in genome maintenance.
Tue Jul 12 00:00:00 UTC 2016
Satb2 determines miRNA expression and long-term memory in the adult central nervous system.
eLife
Jaitner C,Reddy C,Abentung A,Whittle N,Rieder D,Delekate A,Korte M,Jain G,Fischer A,Sananbenesi F,Cera I,Singewald N,Dechant G,Apostolova G
R960-25 was used in Immunohistochemistry to study how the deletion of Satb2 from the adult mouse forebrain affects synaptic long-term potentiation and object discrimination memory.
Tue Nov 29 00:00:00 UTC 2016
Satb2 determines miRNA expression and long-term memory in the adult central nervous system.
eLife
Jaitner C,Reddy C,Abentung A,Whittle N,Rieder D,Delekate A,Korte M,Jain G,Fischer A,Sananbenesi F,Cera I,Singewald N,Dechant G,Apostolova G
R960-25 was used in Immunohistochemistry to study how the deletion of Satb2 from the adult mouse forebrain affects synaptic long-term potentiation and object discrimination memory.
Tue Nov 29 00:00:00 UTC 2016
TRM6/61 connects PKCα with translational control through tRNAi(Met) stabilization: impact on tumorigenesis.
Oncogene
Macari F,El-Houfi Y,Boldina G,Xu H,Khoury-Hanna S,Ollier J,Yazdani L,Zheng G,Bièche I,Legrand N,Paulet D,Durrieu S,Byström A,Delbecq S,Lapeyre B,Bauchet L,Pannequin J,Hollande F,Pan T,Teichmann M,Vagner S,David A,Choquet A,Joubert D
R960-25 was used in Immunoprecipitation to show that protein kinase C α (PKCα) interacts with TRM61, the catalytic subunit of the TRM6/61 tRNA methyltransferase.
Thu Apr 07 00:00:00 UTC 2016
ZEB1 turns into a transcriptional activator by interacting with YAP1 in aggressive cancer types.
Nature communications
Lehmann W,Mossmann D,Kleemann J,Mock K,Meisinger C,Brummer T,Herr R,Brabletz S,Stemmler MP,Brabletz T
R960-25 was used in immunoprecipitation and western blot to study the interaction with YAP1 in aggressive cancer types by ZEB1 turning into a transcriptional activator
Mon Feb 15 00:00:00 UTC 2016
Fascin Rigidity and L-plastin Flexibility Cooperate in Cancer Cell Invadopodia and Filopodia.
The Journal of biological chemistry
Van Audenhove I,Denert M,Boucherie C,Pieters L,Cornelissen M,Gettemans J
R960-25 was used in Western Blot to study the contribution of L-plastin to degradation and invasiveness of cancer cells as a novel component of invadopodia.
Fri Apr 22 00:00:00 UTC 2016
EYA1 expression and subcellular localization in neuroblastoma and its association with prognostic markers.
Journal of cancer research & therapy
Hansen JN,Lotta LT,Eberhardt A,Schor NF,Li X
R960-25 was used in Immunoprecipitation to find that Eyes Absent 1 levels were significantly higher in neuroblastomas than in ganglioneuromas and ganglioneuroblastomas.
Wed Jun 01 00:00:00 UTC 2016
Intrinsic Disorder in Transmembrane Proteins: Roles in Signaling and Topology Prediction.
PloS one
Bürgi J,Xue B,Uversky VN,van der Goot FG
R960-25 was used in immunocytochemistry to study signaling and topology prediction by an intrinsic disorder in transmembrane proteins
Fri Aug 18 00:00:00 UTC 2017
APEX Fingerprinting Reveals the Subcellular Localization of Proteins of Interest.
Cell reports
Lee SY,Kang MG,Park JS,Lee G,Ting AY,Rhee HW
R960-25 was used in immunocytochemistry and western blot to analyze the revelation of subcellular localization of proteins of interest by APEX fingerprinting
Tue May 24 00:00:00 UTC 2016
Biliverdin Reductase A Attenuates Hepatic Steatosis by Inhibition of Glycogen Synthase Kinase (GSK) 3β Phosphorylation of Serine 73 of Peroxisome Proliferator-activated Receptor (PPAR) α.
The Journal of biological chemistry
Hinds TD,Burns KA,Hosick PA,McBeth L,Nestor-Kalinoski A,Drummond HA,AlAmodi AA,Hankins MW,Vanden Heuvel JP,Stec DE
R960-25 was used in immunoprecipitation identify and characterize a biliverdin reductase A-glycogen synthase kinase 3beta-peroxisome proliferator-activated receptor alpha axis that regulates hepatic lipid metabolism
Fri Nov 25 00:00:00 UTC 2016
RIFINs are adhesins implicated in severe Plasmodium falciparum malaria.
Nature medicine
Goel S,Palmkvist M,Moll K,Joannin N,Lara P,Akhouri RR,Moradi N,Öjemalm K,Westman M,Angeletti D,Kjellin H,Lehtiö J,Blixt O,Ideström L,Gahmberg CG,Storry JR,Hult AK,Olsson ML,von Heijne G,Nilsson I,Wahlgren M
R960-25 was used in western blot to examine the contribution of repetitive interspersed families of polypeptides to rosetting during malaria
Wed Apr 01 00:00:00 UTC 2015
PCSK6-mediated corin activation is essential for normal blood pressure.
Nature medicine
Chen S,Cao P,Dong N,Peng J,Zhang C,Wang H,Zhou T,Yang J,Zhang Y,Martelli EE,Naga Prasad SV,Miller RE,Malfait AM,Zhou Y,Wu Q
R960-25 was used in Immunoprecipitation to examine the role of proprotein convertase subtilisin/kexin-6 (PCSK6) cleavage of corin in blood pressure regulation.
Tue Sep 01 00:00:00 UTC 2015
Directed evolution of APEX2 for electron microscopy and proximity labeling.
Nature methods
Lam SS,Martell JD,Kamer KJ,Deerinck TJ,Ellisman MH,Mootha VK,Ting AY
R960-25 was used in Immunofluorescence to study how APEX2 is far more active in cells, enabling the use of electron microscopy to resolve the submitochondrial localization of calcium uptake regulatory protein MICU1.
Thu Jan 01 00:00:00 UTC 2015
The Hippo pathway effectors YAP and TAZ promote cell growth by modulating amino acid signaling to mTORC1.
Cell research
Hansen CG,Ng YL,Lam WL,Plouffe SW,Guan KL
R960-25 was used in Immunocytochemistry-immunoflourescence and Western blot to show that YAP/TAZ play an essential role in amino acid-induced mTORC1 activation, particularly under nutrient-limiting conditions.
Tue Dec 01 00:00:00 UTC 2015
The Hippo pathway effectors YAP and TAZ promote cell growth by modulating amino acid signaling to mTORC1.
Cell research
Hansen CG,Ng YL,Lam WL,Plouffe SW,Guan KL
R960-25 was used in Immunocytochemistry-immunoflourescence and Western blot to show that YAP/TAZ play an essential role in amino acid-induced mTORC1 activation, particularly under nutrient-limiting conditions.
Tue Dec 01 00:00:00 UTC 2015
Cytosolic Nuclease TREX1 Regulates Oligosaccharyltransferase Activity Independent of Nuclease Activity to Suppress Immune Activation.
Immunity
Hasan M,Fermaintt CS,Gao N,Sakai T,Miyazaki T,Jiang S,Li QZ,Atkinson JP,Morse HC,Lehrman MA,Yan N
R960-25 was used in Immunoprecipitation to find that the TREX1 C terminus suppressed immune activation by interacting with the ER oligosaccharyltransferase complex and stabilizing its catalytic integrity.
Tue Sep 15 00:00:00 UTC 2015
Cytosolic Nuclease TREX1 Regulates Oligosaccharyltransferase Activity Independent of Nuclease Activity to Suppress Immune Activation.
Immunity
Hasan M,Fermaintt CS,Gao N,Sakai T,Miyazaki T,Jiang S,Li QZ,Atkinson JP,Morse HC,Lehrman MA,Yan N
R960-25 was used in Immunoprecipitation to find that the TREX1 C terminus suppressed immune activation by interacting with the ER oligosaccharyltransferase complex and stabilizing its catalytic integrity.
Tue Sep 15 00:00:00 UTC 2015
Cytosolic Nuclease TREX1 Regulates Oligosaccharyltransferase Activity Independent of Nuclease Activity to Suppress Immune Activation.
Immunity
Hasan M,Fermaintt CS,Gao N,Sakai T,Miyazaki T,Jiang S,Li QZ,Atkinson JP,Morse HC,Lehrman MA,Yan N
R960-25 was used in Immunoprecipitation to find that the TREX1 C terminus suppressed immune activation by interacting with the ER oligosaccharyltransferase complex and stabilizing its catalytic integrity.
Tue Sep 15 00:00:00 UTC 2015
A recombined allele of the lipase gene CEL and its pseudogene CELP confers susceptibility to chronic pancreatitis.
Nature genetics
Fjeld K,Weiss FU,Lasher D,Rosendahl J,Chen JM,Johansson BB,Kirsten H,Ruffert C,Masson E,Steine SJ,Bugert P,Cnop M,Grützmann R,Mayerle J,Mössner J,Ringdal M,Schulz HU,Sendler M,Simon P,Sztromwasser P,Torsvik J,Scholz M,Tjora E,Férec C,Witt H,Lerch MM,Njølstad PR,Johansson S,Molven A
R960-25 was used in Immunofluorescence to implicate a new pathway distinct from the protease-antiprotease system of pancreatic acinar cells in chronic pancreatitis.
Fri May 01 00:00:00 UTC 2015
A recombined allele of the lipase gene CEL and its pseudogene CELP confers susceptibility to chronic pancreatitis.
Nature genetics
Fjeld K,Weiss FU,Lasher D,Rosendahl J,Chen JM,Johansson BB,Kirsten H,Ruffert C,Masson E,Steine SJ,Bugert P,Cnop M,Grützmann R,Mayerle J,Mössner J,Ringdal M,Schulz HU,Sendler M,Simon P,Sztromwasser P,Torsvik J,Scholz M,Tjora E,Férec C,Witt H,Lerch MM,Njølstad PR,Johansson S,Molven A
R960-25 was used in Immunofluorescence to implicate a new pathway distinct from the protease-antiprotease system of pancreatic acinar cells in chronic pancreatitis.
Fri May 01 00:00:00 UTC 2015
Alternative splicing regulates the expression of G9A and SUV39H2 methyltransferases, and dramatically changes SUV39H2 functions.
Nucleic acids research
Mauger O,Klinck R,Chabot B,Muchardt C,Allemand E,Batsché E
R960-25 was used in Immunocytochemistry-immunoflourescence to investigate how alternative splicing affects the function of two human histone methyltransferases: G9A and SUV39H2.
Wed Feb 18 00:00:00 UTC 2015
Alternative splicing regulates the expression of G9A and SUV39H2 methyltransferases, and dramatically changes SUV39H2 functions.
Nucleic acids research
Mauger O,Klinck R,Chabot B,Muchardt C,Allemand E,Batsché E
R960-25 was used in Immunocytochemistry-immunoflourescence to investigate how alternative splicing affects the function of two human histone methyltransferases: G9A and SUV39H2.
Wed Feb 18 00:00:00 UTC 2015
Recessive mutations in SLC13A5 result in a loss of citrate transport and cause neonatal epilepsy, developmental delay and teeth hypoplasia.
Brain : a journal of neurology
Hardies K,de Kovel CG,Weckhuysen S,Asselbergh B,Geuens T,Deconinck T,Azmi A,May P,Brilstra E,Becker F,Barisic N,Craiu D,Braun KP,Lal D,Thiele H,Schubert J,Weber Y,van 't Slot R,Nürnberg P,Balling R,Timmerman V,Lerche H,Maudsley S,Helbig I,Suls A,Koeleman BP,De Jonghe P
R960-25 was used in Western Blotting to demonstrate that cells expressing mutant sodium-dependent citrate transporter have a complete loss of citrate uptake due to various cellular loss-of-function mechanisms.
Sun Nov 01 00:00:00 UTC 2015
The Eya1 phosphatase promotes Shh signaling during hindbrain development and oncogenesis.
Developmental cell
Eisner A,Pazyra-Murphy MF,Durresi E,Zhou P,Zhao X,Chadwick EC,Xu PX,Hillman RT,Scott MP,Greenberg ME,Segal RA
R960-25 was used in western blot to study Shh signaling during oncogenesis and hindbrain development due to Eya1 phosphate promotion
Mon Apr 06 00:00:00 UTC 2015
ATG16L1 phosphorylation is oppositely regulated by CSNK2/casein kinase 2 and PPP1/protein phosphatase 1 which determines the fate of cardiomyocytes during hypoxia/reoxygenation.
Autophagy
Song H,Pu J,Wang L,Wu L,Xiao J,Liu Q,Chen J,Zhang M,Liu Y,Ni M,Mo J,Zheng Y,Wan D,Cai X,Cao Y,Xiao W,Ye L,Tu E,Lin Z,Wen J,Lu X,He J,Peng Y,Su J,Zhang H,Zhao Y,Lin M,Zhang Z
R960-25 was used in immunoprecipitation to investigate the regulatory mechanism and role of ATG16L1 phosphorylation in autophagy induction using cardiomyocytes.
Mon Jun 20 00:00:00 UTC 2016
ELF5 Drives Lung Metastasis in Luminal Breast Cancer through Recruitment of Gr1+ CD11b+ Myeloid-Derived Suppressor Cells.
PLoS biology
Gallego-Ortega D,Ledger A,Roden DL,Law AM,Magenau A,Kikhtyak Z,Cho C,Allerdice SL,Lee HJ,Valdes-Mora F,Herrmann D,Salomon R,Young AI,Lee BY,Sergio CM,Kaplan W,Piggin C,Conway JR,Rabinovich B,Millar EK,Oakes SR,Chtanova T,Swarbrick A,Naylor MJ,O'Toole S,Green AR,Timpson P,Gee JM,Ellis IO,Clark SJ,Ormandy CJ
R960-25 was used in Western Blotting to implicate ELF5 as a defining factor in the acquisition of the key aspects of the lethal phenotype in luminal A breast cancer.
Tue Dec 01 00:00:00 UTC 2015
Binding of the sphingolipid S1P to hTERT stabilizes telomerase at the nuclear periphery by allosterically mimicking protein phosphorylation.
Science signaling
Panneer Selvam S,De Palma RM,Oaks JJ,Oleinik N,Peterson YK,Stahelin RV,Skordalakes E,Ponnusamy S,Garrett-Mayer E,Smith CD,Ogretmen B
R960-25 was used in Western Blotting to understand the mechanisms of telomerase stabilisation mediated by sphingolipid S1P binding to human telomerase reverse transcriptase.
Tue Jun 16 00:00:00 UTC 2015
Nucleostemin stabilizes ARF by inhibiting the ubiquitin ligase ULF.
Oncogene
Lo D,Zhang Y,Dai MS,Sun XX,Zeng SX,Lu H
R960-25 was used in Western Blotting to study the protein-protein interactions influencing the growth regulatory properties of nucleostemin, independently of p53.
Thu Mar 26 00:00:00 UTC 2015
A secretory kinase complex regulates extracellular protein phosphorylation.
eLife
Cui J,Xiao J,Tagliabracci VS,Wen J,Rahdar M,Dixon JE
R960-25 was used in Western Blotting to show that Fam20A potentiates Fam20C kinase activity and promotes the phosphorylation of enamel matrix proteins in vitro and in cells.
Thu Mar 19 00:00:00 UTC 2015
Dengue Virus Impairs Mitochondrial Fusion by Cleaving Mitofusins.
PLoS pathogens
Yu CY,Liang JJ,Li JK,Lee YL,Chang BL,Su CI,Huang WJ,Lai MM,Lin YL
R960-25 was used in immunoprecipitation to characterize how dengue virus cleaves mitofusins and impairs mitochondrial fusion
Tue Dec 01 00:00:00 UTC 2015
Inactivation of the Human Cytomegalovirus US20 Gene Hampers Productive Viral Replication in Endothelial Cells.
Journal of virology
Cavaletto N,Luganini A,Gribaudo G
R960-25 was used in immunocytochemistry and western blot to study the inactivation of the human cytomegalovirus US20 gene impedes viral replication in endothelial cells
Sun Nov 01 00:00:00 UTC 2015
Role of Acinus in regulating retinoic acid-responsive gene pre-mRNA splicing.
Journal of cellular physiology
Wang F,Soprano KJ,Soprano DR
R960-25 was used in western blot to study retinoic acid-responsive gene pre-mRNA splicing regulated by Acinus
Wed Apr 01 00:00:00 UTC 2015
Tyrosine phosphorylation of RAS by ABL allosterically enhances effector binding.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Ting PY,Johnson CW,Fang C,Cao X,Graeber TG,Mattos C,Colicelli J
R960-25 was used in Immunoprecipitation to identify Abelson tyrosine-protein kinase substrates downstream of the RAS-to-RIN1 signaling pathway in human HEK293T cells.
Tue Sep 01 00:00:00 UTC 2015
Tyrosine phosphorylation of RAS by ABL allosterically enhances effector binding.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Ting PY,Johnson CW,Fang C,Cao X,Graeber TG,Mattos C,Colicelli J
R960-25 was used in Immunoprecipitation to identify Abelson tyrosine-protein kinase substrates downstream of the RAS-to-RIN1 signaling pathway in human HEK293T cells.
Tue Sep 01 00:00:00 UTC 2015
Efficient use of a translation start codon in BDNF exon I.
Journal of neurochemistry
Koppel I,Tuvikene J,Lekk I,Timmusk T
R960-25 was used in western blot to investigate translation of brain-derived neurotrophic factor.
Tue Sep 01 00:00:00 UTC 2015
Ndfip1 regulates itch ligase activity and airway inflammation via UbcH7.
Journal of immunology (Baltimore, Md. : 1950)
Kathania M,Zeng M,Yadav VN,Moghaddam SJ,Yang B,Venuprasad K
R960-25 was used in immunoprecipitation and western blot to provide mechanistic insights into how Itch function is regulated during inflammatory signaling
Sun Mar 01 00:00:00 UTC 2015
The caspase-3-p120-RasGAP module generates a NF-κB repressor in response to cellular stress.
Journal of cell science
Khalil H,Loukili N,Regamey A,Cuesta-Marban A,Santori E,Huber M,Widmann C
R960-25 was used in immunoprecipitation and western blot to study the role of caspase-3-p120-RasGAP in cellular stress response
Tue Sep 15 00:00:00 UTC 2015
Evolutionarily conserved pressure for the existence of distinct G2/M cell cycle arrest and A3H inactivation functions in HIV-1 Vif.
Cell cycle (Georgetown, Tex.)
Zhao K,Du J,Rui Y,Zheng W,Kang J,Hou J,Wang K,Zhang W,Simon VA,Yu XF
R960CUS was used in Western Blot to identify distinct yet overlapping domains that are required for HIV-1 Vif-mediated G2/M-phase cell cycle arrest and APOBEC3H degradation, but not for the inactivation of APOBEC3G or APOBEC3F.
Tue Dec 15 00:00:00 UTC 2015
Loss of fibulin-4 disrupts collagen synthesis and maturation: implications for pathology resulting from EFEMP2 mutations.
Human molecular genetics
Papke CL,Tsunezumi J,Ringuette LJ,Nagaoka H,Terajima M,Yamashiro Y,Urquhart G,Yamauchi M,Davis EC,Yanagisawa H
R960-25 was used in immunoprecipitation to identify the underlying disease mechanism that result from EFEMP2 mutations
Thu Oct 15 00:00:00 UTC 2015
Dominant, toxic gain-of-function mutations in gars lead to non-cell autonomous neuropathology.
Human molecular genetics
Grice SJ,Sleigh JN,Motley WW,Liu JL,Burgess RW,Talbot K,Cader MZ
R960-25 was used in Western Blotting to study how dominant mutations in glycyl-tRNA synthetase allow access to ectopic sub-compartments of the motor neuron in Charcot-Marie-Tooth disease type 2D.
Sat Aug 01 00:00:00 UTC 2015
ZC4H2, an XLID gene, is required for the generation of a specific subset of CNS interneurons.
Human molecular genetics
May M,Hwang KS,Miles J,Williams C,Niranjan T,Kahler SG,Chiurazzi P,Steindl K,Van Der Spek PJ,Swagemakers S,Mueller J,Stefl S,Alexov E,Ryu JI,Choi JH,Kim HT,Tarpey P,Neri G,Holloway L,Skinner C,Stevenson RE,Dorsky RI,Wang T,Schwartz CE,Kim CH
R960-25 was used in immunocytochemistry to analysis of an XLID gene, ZC4H2, that is required for generation of a specific subset of CNS interneurons
Tue Sep 01 00:00:00 UTC 2015
Histone Deacetylase 10 Regulates the Cell Cycle G2/M Phase Transition via a Novel Let-7-HMGA2-Cyclin A2 Pathway.
Molecular and cellular biology
Li Y,Peng L,Seto E
R960-25 was used in Chromatin immunoprecipitation to report HDAC10 regulates the cell cycle via cyclin A2 expression modulation, and cyclin A2 overexpression rescues HDAC10 knockdown-induced G2/M transition arrest.
Thu Oct 01 00:00:00 UTC 2015
UCR1C is a novel activator of phosphodiesterase 4 (PDE4) long isoforms and attenuates cardiomyocyte hypertrophy.
Cellular signalling
Wang L,Burmeister BT,Johnson KR,Baillie GS,Karginov AV,Skidgel RA,O'Bryan JP,Carnegie GK
R960-25 was used in Western Blotting to demonstrate that PDE4 activation by UCR1C attenuates cardiomyocyte hypertrophy by specifically inhibiting nuclear PKA activity.
Fri May 01 00:00:00 UTC 2015
UCR1C is a novel activator of phosphodiesterase 4 (PDE4) long isoforms and attenuates cardiomyocyte hypertrophy.
Cellular signalling
Wang L,Burmeister BT,Johnson KR,Baillie GS,Karginov AV,Skidgel RA,O'Bryan JP,Carnegie GK
R960-25 was used in Western Blotting to demonstrate that PDE4 activation by UCR1C attenuates cardiomyocyte hypertrophy by specifically inhibiting nuclear PKA activity.
Fri May 01 00:00:00 UTC 2015
Evolutionarily conserved exon definition interactions with U11 snRNP mediate alternative splicing regulation on U11-48K and U11/U12-65K genes.
RNA biology
Niemelä EH,Verbeeren J,Singha P,Nurmi V,Frilander MJ
R960-25 was used in Immunocytochemistry to describe an autoregulatory feedback mechanism that regulates two core protein components of the U12-dependent spliceosome.
Thu Sep 15 00:00:00 UTC 2016
Evolutionarily conserved exon definition interactions with U11 snRNP mediate alternative splicing regulation on U11-48K and U11/U12-65K genes.
RNA biology
Niemelä EH,Verbeeren J,Singha P,Nurmi V,Frilander MJ
R960-25 was used in Immunocytochemistry to describe an autoregulatory feedback mechanism that regulates two core protein components of the U12-dependent spliceosome.
Thu Sep 15 00:00:00 UTC 2016
Evolutionarily conserved exon definition interactions with U11 snRNP mediate alternative splicing regulation on U11-48K and U11/U12-65K genes.
RNA biology
Niemelä EH,Verbeeren J,Singha P,Nurmi V,Frilander MJ
R960-25 was used in Immunocytochemistry to describe an autoregulatory feedback mechanism that regulates two core protein components of the U12-dependent spliceosome.
Thu Sep 15 00:00:00 UTC 2016
A simple and efficient method for transfecting mouse embryonic stem cells using polyethylenimine.
Experimental cell research
Bartman CM,Egelston J,Ren X,Das R,Phiel CJ
R960-25 was used in Western Blotting to provide evidence that polyethylenimine transfection of mouse embryonic stem cells compares favourably to Lipofectamine2000.
Thu Jan 01 00:00:00 UTC 2015
Snail-mediated Cripto-1 repression regulates the cell cycle and epithelial-mesenchymal transition-related gene expression.
FEBS letters
Pilli VS,Gupta K,Kotha BP,Aradhyam GK
R960-25 was used in western blot to investigate the role of Snail in the cell cycle regulation and epithelial-mesenchymal transition
Fri May 08 00:00:00 UTC 2015
A Novel Role of Salt-Inducible Kinase 1 (SIK1) in the Post-Translational Regulation of Scavenger Receptor Class B Type 1 Activity.
Biochemistry
Hu Z,Hu J,Shen WJ,Kraemer FB,Azhar S
R960-25 was used in immunoprecipitation to analyze post-translational regulation of scavenger receptor class B type 1 activity by salt-inducible kinase 1 (SIK1)
Tue Nov 24 00:00:00 UTC 2015
Differential coexpression of FoxP1, FoxP2, and FoxP4 in the Zebra Finch (Taeniopygia guttata) song system.
The Journal of comparative neurology
Mendoza E,Tokarev K,Düring DN,Retamosa EC,Weiss M,Arpenik N,Scharff C
R960-25 was used in western blot to assess if transcriptional activity of FoxP2 requires dimerization with itself or with paralogs FoxP1 and FoxP4 in vivo
Mon Jun 15 00:00:00 UTC 2015
A Polybasic Plasma Membrane Binding Motif in the I-II Linker Stabilizes Voltage-gated CaV1.2 Calcium Channel Function.
The Journal of biological chemistry
Kaur G,Pinggera A,Ortner NJ,Lieb A,Sinnegger-Brauns MJ,Yarov-Yarovoy V,Obermair GJ,Flucher BE,Striessnig J
R960-25 was used in immunocytochemistry to study the role of the polybasic plasma membrane binding motif in Cav1.2 channel function
Fri Aug 21 00:00:00 UTC 2015
Caspase-dependent Proteolysis of Human Ribonucleotide Reductase Small Subunits R2 and p53R2 during Apoptosis.
The Journal of biological chemistry
Tebbi A,Guittet O,Tuphile K,Cabrié A,Lepoivre M
R960-25 was used in Western Blotting to identify ribonucleotide reductase activity as a prosurvival function inactivated by proteolysis during apoptosis.
Fri May 29 00:00:00 UTC 2015
Molecular Determinants for the Inactivation of the Retinoblastoma Tumor Suppressor by the Viral Cyclin-dependent Kinase UL97.
The Journal of biological chemistry
Iwahori S,Hakki M,Chou S,Kalejta RF
R960-25 was used in Western Blotting to demonstrate that UL97 phosphorylates 11 of the 16 consensus cyclin-dependent kinase sites in retinoblastoma.
Fri Aug 07 00:00:00 UTC 2015
Localization of Hippo signalling complexes and Warts activation in vivo.
Nature communications
Sun S,Reddy BV,Irvine KD
R960-25 was used in immunohistochemistry to characterize Warts activation and localization of Hippo signalling in vivo
Wed Sep 30 00:00:00 UTC 2015
Myosin 1b Regulates Amino Acid Transport by Associating Transporters with the Apical Plasma Membrane of Kidney Cells.
PloS one
Komaba S,Coluccio LM
R960-25 was used in Immunofluorescence to define a physiological role for Myo1b, that of regulating renal AAT by modulating the association of AATers with the APM.
Mon Jun 06 00:00:00 UTC 2016
20-Hydroxyecdysone (20E) Primary Response Gene E93 Modulates 20E Signaling to Promote Bombyx Larval-Pupal Metamorphosis.
The Journal of biological chemistry
Liu X,Dai F,Guo E,Li K,Ma L,Tian L,Cao Y,Zhang G,Palli SR,Li S
R960-25 was used in Immunoprecipitation to examine the transcriptional modulation of 20-Hydroxyecdysone by the primary response gene, E93, in Bombyx larval-pupal metamorphosis.
Fri Nov 06 00:00:00 UTC 2015
Comprehensive functional characterization of cancer-testis antigens defines obligate participation in multiple hallmarks of cancer.
Nature communications
Maxfield KE,Taus PJ,Corcoran K,Wooten J,Macion J,Zhou Y,Borromeo M,Kollipara RK,Yan J,Xie Y,Xie XJ,Whitehurst AW
R960-25 was used in western blot to assess the definition of obligate participation in multiple hallmarks of cancer by a comprehensive functional characterization of cancer-testis antigens
Mon Nov 16 00:00:00 UTC 2015
Differential Editosome Protein Function between Life Cycle Stages of Trypanosoma brucei.
The Journal of biological chemistry
McDermott SM,Guo X,Carnes J,Stuart K
R960-25 was used in Western Blotting to indicate that the functions of KREPB5 and KREPA3 editosome proteins are adjusted between the Trypanosoma brucei life cycle stages.
Fri Oct 09 00:00:00 UTC 2015
DNA damage induces GDNF secretion in the tumor microenvironment with paracrine effects promoting prostate cancer treatment resistance.
Oncotarget
Huber RM,Lucas JM,Gomez-Sarosi LA,Coleman I,Zhao S,Coleman R,Nelson PS
R960-25 was used in western blot to study how glial cell line-derived neurotrophic factor promotes prostate cancer growth.
Tue Feb 10 00:00:00 UTC 2015
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions.
Journal of visualized experiments : JoVE
Fasolo J,Im H,Snyder MP
R960-25 was used in ELISA to study kinase protein-protein interactions.
Sun Aug 02 00:00:00 UTC 2015
Recruitment of Matrix Metalloproteinase-9 (MMP-9) to the Fibroblast Cell Surface by Lysyl Hydroxylase 3 (LH3) Triggers Transforming Growth Factor-β (TGF-β) Activation and Fibroblast Differentiation.
The Journal of biological chemistry
Dayer C,Stamenkovic I
R960-25 was used in Flow cytometry/Cell sorting to uncover LH3 as a new docking receptor of MMP-9 on the fibroblast cell surface and demonstrate that the MMP-9 FN domain is essential for the interaction.
Fri May 29 00:00:00 UTC 2015
Recruitment of Matrix Metalloproteinase-9 (MMP-9) to the Fibroblast Cell Surface by Lysyl Hydroxylase 3 (LH3) Triggers Transforming Growth Factor-β (TGF-β) Activation and Fibroblast Differentiation.
The Journal of biological chemistry
Dayer C,Stamenkovic I
R960-25 was used in Flow cytometry/Cell sorting to uncover LH3 as a new docking receptor of MMP-9 on the fibroblast cell surface and demonstrate that the MMP-9 FN domain is essential for the interaction.
Fri May 29 00:00:00 UTC 2015
Recruitment of Matrix Metalloproteinase-9 (MMP-9) to the Fibroblast Cell Surface by Lysyl Hydroxylase 3 (LH3) Triggers Transforming Growth Factor-β (TGF-β) Activation and Fibroblast Differentiation.
The Journal of biological chemistry
Dayer C,Stamenkovic I
R960-25 was used in Flow cytometry/Cell sorting to uncover LH3 as a new docking receptor of MMP-9 on the fibroblast cell surface and demonstrate that the MMP-9 FN domain is essential for the interaction.
Fri May 29 00:00:00 UTC 2015
Profilin 1 with the amyotrophic lateral sclerosis associated mutation T109M displays unaltered actin binding and does not affect the actin cytoskeleton.
BMC neuroscience
Freischmidt A,Schöpflin M,Feiler MS,Fleck AK,Ludolph AC,Weishaupt JH
R960-25 was used in immunocytochemistry and immunoprecipitation to determine the display of unaltered actin binding and lack of affect on the actin cytoskeleton by profilin 1 with amyotrophic lateral sclerosis associated mutation T109M
Mon Nov 16 00:00:00 UTC 2015
ABCE1 is a highly conserved RNA silencing suppressor.
PloS one
Kärblane K,Gerassimenko J,Nigul L,Piirsoo A,Smialowska A,Vinkel K,Kylsten P,Ekwall K,Swoboda P,Truve E,Sarmiento C
R960-25 was used in Immunoprecipitation to report suppression of RNA silencing is conserved function of ABCE1 in Nicotiana benthamiana, mammalian HEK293 cells and Caenorhabditis elegans.
Fri Jan 08 00:00:00 UTC 2016
The brown adipocyte protein CIDEA promotes lipid droplet fusion via a phosphatidic acid-binding amphipathic helix.
eLife
Barneda D,Planas-Iglesias J,Gaspar ML,Mohammadyani D,Prasannan S,Dormann D,Han GS,Jesch SA,Carman GM,Kagan V,Parker MG,Ktistakis NT,Klein-Seetharaman J,Dixon AM,Henry SA,Christian M
R960-25 was used in immunoprecipitation to study promotion of lipid droplet fusion via a phosphatidic acid-binding amphipathic helix by brown adipocyte protein CIDEA
Thu Nov 26 00:00:00 UTC 2015
Protein Kinase A Opposes the Phosphorylation-dependent Recruitment of Glycogen Synthase Kinase 3β to A-kinase Anchoring Protein 220.
The Journal of biological chemistry
Whiting JL,Nygren PJ,Tunquist BJ,Langeberg LK,Seternes OM,Scott JD
R960-25 was used in Chromatin immunoprecipitation to show that glycogen synthase kinase 3Β phosphorylation of Thr-1132 on A-kinase anchoring protein 220 initiates recruitment of this kinase into the enzyme scaffold.
Fri Aug 07 00:00:00 UTC 2015
Massive parallel sequencing uncovers actionable FGFR2-PPHLN1 fusion and ARAF mutations in intrahepatic cholangiocarcinoma.
Nature communications
Sia D,Losic B,Moeini A,Cabellos L,Hao K,Revill K,Bonal D,Miltiadous O,Zhang Z,Hoshida Y,Cornella H,Castillo-Martin M,Pinyol R,Kasai Y,Roayaie S,Thung SN,Fuster J,Schwartz ME,Waxman S,Cordon-Cardo C,Schadt E,Mazzaferro V,Llovet JM
R960-25 was used in Immunoprecipitation to identify actionable molecular characteristics associated with Intrahepatic cholangiocarcinoma (iCCA) for therapeutic targeting.
Thu Jan 22 00:00:00 UTC 2015
Structural basis for mutation-induced destabilization of profilin 1 in ALS.
Proceedings of the National Academy of Sciences of the United States of America
Boopathy S,Silvas TV,Tischbein M,Jansen S,Shandilya SM,Zitzewitz JA,Landers JE,Goode BL,Schiffer CA,Bosco DA
R960-25 was used in Western Blotting to suggest that a destabilized form of PFN1 underlies PFN1-mediated ALS pathogenesis.
Tue Jun 30 00:00:00 UTC 2015
Differential expression of epithelial basement membrane components nidogens and perlecan in corneal stromal cells in vitro.
Molecular vision
Santhanam A,Torricelli AA,Wu J,Marino GK,Wilson SE
R960-25 was used in western blot to determine the expression of corneal epithelial basement membrane components in different corneal stromal cell types
Mon Sep 26 00:00:00 UTC 2016
Signaling pathway models as biomarkers: Patient-specific simulations of JNK activity predict the survival of neuroblastoma patients.
Science signaling
Fey D,Halasz M,Dreidax D,Kennedy SP,Hastings JF,Rauch N,Munoz AG,Pilkington R,Fischer M,Westermann F,Kolch W,Kholodenko BN,Croucher DR
R960-25 was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot to indicate that patient-specific simulations of JNK activation could stratify patients.
Tue Dec 22 00:00:00 UTC 2015
The Inhibitory Core of the Myostatin Prodomain: Its Interaction with Both Type I and II Membrane Receptors, and Potential to Treat Muscle Atrophy.
PloS one
Ohsawa Y,Takayama K,Nishimatsu S,Okada T,Fujino M,Fukai Y,Murakami T,Hagiwara H,Itoh F,Tsuchida K,Hayashi Y,Sunada Y
R960-25 was used in immunocytochemistry and immunoprecipitation to study the therapeutic use of the inhibitory core of the prodomain of myostatin
Wed May 04 00:00:00 UTC 2016
Differential dependence on N-glycosylation of anthrax toxin receptors CMG2 and TEM8.
PloS one
Friebe S,Deuquet J,van der Goot FG
R960-25 was used in immunoprecipitation to determine the importance of N-glycosylation on the folding, trafficking and ligand binding of ANTXR 1 and 2.
Tue Mar 15 00:00:00 UTC 2016
mab21-l3 regulates cell fate specification of multiciliate cells and ionocytes.
Nature communications
Takahashi C,Kusakabe M,Suzuki T,Miyatake K,Nishida E
R960-25 was used in Immunohistochemistry-wholemount to indicate that mab21-l3 acts downstream of the Notch pathway in cell fate specifications of multiciliate cells and ionocytes.
Mon Jan 19 00:00:00 UTC 2015
Nuclear DICKKOPF-1 as a biomarker of chemoresistance and poor clinical outcome in colorectal cancer.
Oncotarget
Aguilera Ó,González-Sancho JM,Zazo S,Rincón R,Fernández AF,Tapia O,Canals F,Morte B,Calvanese V,Orgaz JL,Niell N,Aguilar S,Freije JM,Graña O,Pisano DG,Borrero A,Martínez-Useros J,Jiménez B,Fraga MF,García-Foncillas J,López-Otín C,Lafarga M,Rojo F,Muñoz A
R960-25 was used in immunocytochemistry to show that nuclear DKK-1 is a predictive biomarker of chemoresistance in colorectal cancer
Fri Mar 20 00:00:00 UTC 2015
Novel transcripts reveal a complex structure of the human TRKA gene and imply the presence of multiple protein isoforms.
BMC neuroscience
Luberg K,Park R,Aleksejeva E,Timmusk T
R960-25 was used in immunocytochemistry, immunoprecipitation, and western blot to characterize the TRKA gene and its novel transcripts and multiple protein isoforms
Wed Nov 18 00:00:00 UTC 2015
Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.
The Journal of biological chemistry
van der Kant R,Jonker CT,Wijdeven RH,Bakker J,Janssen L,Klumperman J,Neefjes J
R960-25 was used in Western Blotting to provide a detailed analysis of interactions within the mammalian CORVET and HOPS as well as an additional endosomal-targeting complex (VIPAS39-VPS33B) that does not exist in yeast.
Fri Dec 18 00:00:00 UTC 2015
Targeted siRNA Screens Identify ER-to-Mitochondrial Calcium Exchange in Autophagy and Mitophagy Responses in RPE1 Cells.
International journal of molecular sciences
MacVicar TD,Mannack LV,Lees RM,Lane JD
R960-25 was used in Western Blotting to suggest that Ca2+ flux between endoplasmic reticulum and mitochondria is needed both for starvation-induced autophagy and for Parkin-mediated mitophagy.
Thu Jun 11 00:00:00 UTC 2015
Interplay between arginine methylation and ubiquitylation regulates KLF4-mediated genome stability and carcinogenesis.
Nature communications
Hu D,Gur M,Zhou Z,Gamper A,Hung MC,Fujita N,Lan L,Bahar I,Wan Y
R960-25 was used in western blot to study the relationship between arginine methylation and ubiquitylation in carcinogenesis and KLF4-mediated stability
Wed Sep 30 00:00:00 UTC 2015
A functional comparison of the domestic cat bitter receptors Tas2r38 and Tas2r43 with their human orthologs.
BMC neuroscience
Sandau MM,Goodman JR,Thomas A,Rucker JB,Rawson NE
R960-25 was used in flow cytometry to investigate domestic cat taste perception
Wed Jun 03 00:00:00 UTC 2015
Accumulation of the PX domain mutant Frank-ter Haar syndrome protein Tks4 in aggresomes.
Cell communication and signaling : CCS
Ádám C,Fekete A,Bőgel G,Németh Z,Tőkési N,Ovádi J,Liliom K,Pesti S,Geiszt M,Buday L
R960-25 was used in immunocytochemistry and western blot to assess aggresomes with an accumulation of the PX domain mutant Frank-ter Haar syndrome protein Tks4
Fri Jul 17 00:00:00 UTC 2015
Circulating angiopoietin-like 4 links proteinuria with hypertriglyceridemia in nephrotic syndrome.
Nature medicine
Clement LC,Macé C,Avila-Casado C,Joles JA,Kersten S,Chugh SS
R960-25 was used in Western Blotting to show that plasma angiopoietin-like 4 links proteinuria with hypertriglyceridemia through two negative feedback loops.
Wed Jan 01 00:00:00 UTC 2014
Non-cell-autonomous driving of tumour growth supports sub-clonal heterogeneity.
Nature
Marusyk A,Tabassum DP,Altrock PM,Almendro V,Michor F,Polyak K
R960-25 was used in immunohistochemistry - paraffin section to use a mouse xenograft model to investigate the impact of sub-clonal heterogeneity on tumor phenotypes
Thu Oct 02 00:00:00 UTC 2014
Cytoskeletal tension inhibits Hippo signaling through an Ajuba-Warts complex.
Cell
Rauskolb C,Sun S,Sun G,Pan Y,Irvine KD
R960-25 was used in Immunohistochemistry to delineate a mechanism linking cytoskeletal tension to regulation of the Hippo pathway, modulating organ growth in Drosophila.
Thu Jul 03 00:00:00 UTC 2014
Exome-wide association study identifies a TM6SF2 variant that confers susceptibility to nonalcoholic fatty liver disease.
Nature genetics
Kozlitina J,Smagris E,Stender S,Nordestgaard BG,Zhou HH,Tybjærg-Hansen A,Vogt TF,Hobbs HH,Cohen JC
R960-25 was used in western blot to discover a TM6SF2 variant by an exome-wide association study that validates the susceptibility to nonalcoholic fatty liver disease
Tue Apr 01 00:00:00 UTC 2014
A specific missense mutation in GTF2I occurs at high frequency in thymic epithelial tumors.
Nature genetics
Petrini I,Meltzer PS,Kim IK,Lucchi M,Park KS,Fontanini G,Gao J,Zucali PA,Calabrese F,Favaretto A,Rea F,Rodriguez-Canales J,Walker RL,Pineda M,Zhu YJ,Lau C,Killian KJ,Bilke S,Voeller D,Dakshanamurthy S,Wang Y,Giaccone G
R960-25 was used in Western Blotting to identify a missense mutation (chromosome 7 c.74146970T>A) in GTF2I at high frequency in type A thymomas, a relatively indolent subtype.
Fri Aug 01 00:00:00 UTC 2014
NUP98-PHF23 is a chromatin-modifying oncoprotein that causes a wide array of leukemias sensitive to inhibition of PHD histone reader function.
Cancer discovery
Gough SM,Lee F,Yang F,Walker RL,Zhu YJ,Pineda M,Onozawa M,Chung YJ,Bilke S,Wagner EK,Denu JM,Ning Y,Xu B,Wang GG,Meltzer PS,Aplan PD
R960-25 was used in Western Blotting to show that expression of a NUP98-PHF23 (NP23) fusion, associated with acute myeloid leukemia (AML) in humans, leads to myeloid, erythroid, T-cell, and B-cell leukemia in mice.
Thu May 01 00:00:00 UTC 2014
Molecular profiling of the residual disease of triple-negative breast cancers after neoadjuvant chemotherapy identifies actionable therapeutic targets.
Cancer discovery
Balko JM,Giltnane JM,Wang K,Schwarz LJ,Young CD,Cook RS,Owens P,Sanders ME,Kuba MG,Sánchez V,Kurupi R,Moore PD,Pinto JA,Doimi FD,Gómez H,Horiuchi D,Goga A,Lehmann BD,Bauer JA,Pietenpol JA,Ross JS,Palmer GA,Yelensky R,Cronin M,Miller VA,Stephens PJ,Arteaga CL
R960-25 was used in western blot to study treatment resistance mechanisms in triple-negative breast cancers.
Sat Feb 01 00:00:00 UTC 2014
Active, phosphorylated fingolimod inhibits histone deacetylases and facilitates fear extinction memory.
Nature neuroscience
Hait NC,Wise LE,Allegood JC,O'Brien M,Avni D,Reeves TM,Knapp PE,Lu J,Luo C,Miles MF,Milstien S,Lichtman AH,Spiegel S
R960-25 was used in Western Blotting to assess the role of fingolimod on histone acetlyation in the brain, and the role this may play in fear extinction memory functions.
Tue Jul 01 00:00:00 UTC 2014
Rsp5/Nedd4 is the main ubiquitin ligase that targets cytosolic misfolded proteins following heat stress.
Nature cell biology
Fang NN,Chan GT,Zhu M,Comyn SA,Persaud A,Deshaies RJ,Rotin D,Gsponer J,Mayor T
R960-25 was used in Western Blotting to study how misfolded proteins are targeted for proteolysis in heat shock.
Mon Dec 01 00:00:00 UTC 2014
Plasma membrane translocation of trimerized MLKL protein is required for TNF-induced necroptosis.
Nature cell biology
Cai Z,Jitkaew S,Zhao J,Chiang HC,Choksi S,Liu J,Ward Y,Wu LG,Liu ZG
R960-25 was used in Immunocytochemistry-immunoflourescence to report that MLKL forms a homotrimer through its amino-terminal coiled-coil domain and locates to the cell plasma membrane during TNF-induced necroptosis.
Wed Jan 01 00:00:00 UTC 2014
β-arrestin1-biased β1-adrenergic receptor signaling regulates microRNA processing.
Circulation research
Kim IM,Wang Y,Park KM,Tang Y,Teoh JP,Vinson J,Traynham CJ,Pironti G,Mao L,Su H,Johnson JA,Koch WJ,Rockman HA
R960-25 was used in Western Blotting to indicate a novel function for Β1AR-mediated Β-arrestin1 signalling activated by carvedilol in miRNA biogenesis.
Fri Feb 28 00:00:00 UTC 2014
Discovery of protein interactions using parallel analysis of translated ORFs (PLATO).
Nature protocols
Larman HB,Liang AC,Elledge SJ,Zhu J
R960-25 was used in western blot to use parallel analysis of translated ORFs (PLATO) to discover protein interactions
Wed Jan 01 00:00:00 UTC 2014
Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation.
The EMBO journal
Voss M,Künzel U,Higel F,Kuhn PH,Colombo A,Fukumori A,Haug-Kröper M,Klier B,Grammer G,Seidl A,Schröder B,Obst R,Steiner H,Lichtenthaler SF,Haass C,Fluhrer R
R960-25 was used in western blot to that SPPL3 alters the pattern of cellular N-glycosylation.
Wed Dec 17 00:00:00 UTC 2014
Ibf1 and Ibf2 are novel CP190-interacting proteins required for insulator function.
The EMBO journal
Cuartero S,Fresán U,Reina O,Planet E,Espinàs ML
R960-25 was used in western blot to identify novel pathway of CP190 chromatin recruitment.
Tue Mar 18 00:00:00 UTC 2014
Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase.
Genes & development
Zheng X,Zhai B,Koivunen P,Shin SJ,Lu G,Liu J,Geisen C,Chakraborty AA,Moslehi JJ,Smalley DM,Wei X,Chen X,Chen Z,Beres JM,Zhang J,Tsao JL,Brenner MC,Zhang Y,Fan C,DePinho RA,Paik J,Gygi SP,Kaelin WG,Zhang Q
R960-25 was used in Western Blotting to provide new insights into post-transcriptional control of FOXO3a and provide a new avenue for pharmacologically altering Cyclin D1 activity.
Tue Jul 01 00:00:00 UTC 2014
Heterodimerization of p45-p75 modulates p75 signaling: structural basis and mechanism of action.
PLoS biology
Vilar M,Sung TC,Chen Z,García-Carpio I,Fernandez EM,Xu J,Riek R,Lee KF
R960-25 was used in Immunoprecipitation to show that p45 markedly interferes with the function of p75 as a co-receptor for Nogo receptor.
Fri Aug 01 00:00:00 UTC 2014
SUMOylation inhibits FOXM1 activity and delays mitotic transition.
Oncogene
Myatt SS,Kongsema M,Man CW,Kelly DJ,Gomes AR,Khongkow P,Karunarathna U,Zona S,Langer JK,Dunsby CW,Coombes RC,French PM,Brosens JJ,Lam EW
R960-25 was used in western blot to investigate the role of SUMOylation on FOXM1 activity.
Thu Aug 21 00:00:00 UTC 2014
Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.
The Journal of cell biology
Cherepanova NA,Shrimal S,Gilmore R
R960-25 was used in western blot to determine how N-glycosylation of cysteine-proximal acceptor sites in glycoproteins requires oxidoreductase activity
Mon Aug 18 00:00:00 UTC 2014
CD82 regulates STAT5/IL-10 and supports survival of acute myelogenous leukemia cells.
International journal of cancer
Nishioka C,Ikezoe T,Yang J,Nobumoto A,Kataoka S,Tsuda M,Udaka K,Yokoyama A
R960-25 was used in Flow cytometry/Cell sorting to perform a functional analysis of CD82 in CD34(+) /CD38(-) acute myelogenous leukaemia cells.
Wed Jan 01 00:00:00 UTC 2014
A new domain in the Toll/IL-1R domain-containing adaptor inducing interferon-β factor protein amino terminus is important for tumor necrosis factor-α receptor-associated factor 3 association, protein stabilization and interferon signaling.
Journal of innate immunity
Nguyen VP,Chen J,Petrus MN,Goldman CK,Kruhlak MJ,Bamford RN,Waldmann TA
R960-25 was used in Immunoprecipitation to investigate the impact of a 21-residue deletion from the amino-terminal of Toll/IL-1R domain-containing adaptor inducing interferon-Β factor (TRIF) on its function.
Mon May 11 00:00:00 UTC 2015
A new domain in the Toll/IL-1R domain-containing adaptor inducing interferon-β factor protein amino terminus is important for tumor necrosis factor-α receptor-associated factor 3 association, protein stabilization and interferon signaling.
Journal of innate immunity
Nguyen VP,Chen J,Petrus MN,Goldman CK,Kruhlak MJ,Bamford RN,Waldmann TA
R960-25 was used in Immunoprecipitation to investigate the impact of a 21-residue deletion from the amino-terminal of Toll/IL-1R domain-containing adaptor inducing interferon-Β factor (TRIF) on its function.
Mon May 11 00:00:00 UTC 2015
Dlg5 regulates dendritic spine formation and synaptogenesis by controlling subcellular N-cadherin localization.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Wang SH,Celic I,Choi SY,Riccomagno M,Wang Q,Sun LO,Mitchell SP,Vasioukhin V,Huganir RL,Kolodkin AL
R960-25 was used in Immunohistochemistry to show that DLG5 is a MAGUK protein that regulates spine formation, synaptogenesis, and synaptic transmission in cortical neurons.
Wed Sep 17 00:00:00 UTC 2014
Identification of human cytomegalovirus genes important for biogenesis of the cytoplasmic virion assembly complex.
Journal of virology
Das S,Ortiz DA,Gurczynski SJ,Khan F,Pellett PE
R960-25 was used in Immunocytochemistry to identify human cytomegalovirus proteins that regulate cytoplasmic virion assembly complex biogenesis.
Fri Aug 01 00:00:00 UTC 2014
Identification of human cytomegalovirus genes important for biogenesis of the cytoplasmic virion assembly complex.
Journal of virology
Das S,Ortiz DA,Gurczynski SJ,Khan F,Pellett PE
R960-25 was used in Immunocytochemistry to identify human cytomegalovirus proteins that regulate cytoplasmic virion assembly complex biogenesis.
Fri Aug 01 00:00:00 UTC 2014
Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Journal of virology
Jefferson M,Donaszi-Ivanov A,Pollen S,Dalmay T,Saalbach G,Powell PP
R960-25 was used in Immunocytochemistry-immunoflourescence to identify a new role for the viral N-terminal protease through its interaction with over 55 associated proteins, mainly ribosomal proteins and ribonucleoproteins.
Mon Sep 01 00:00:00 UTC 2014
Induced overexpression of OCT4A in human embryonic stem cells increases cloning efficiency.
American journal of physiology. Cell physiology
Tsai SC,Chang DF,Hong CM,Xia P,Senadheera D,Trump L,Mishra S,Lutzko C
R960-25 was used in Immunofluorescence to indicate that high levels of OCT4A increase human embryonic stem cell cloning efficiency and do not induce differentiation.
Sun Jun 15 00:00:00 UTC 2014
The role of the plexin-A2 receptor in Sema3A and Sema3B signal transduction.
Journal of cell science
Sabag AD,Smolkin T,Mumblat Y,Ueffing M,Kessler O,Gloeckner CJ,Neufeld G
R960-25 was used in Western blotting following immunoprecipitation to explore the roles of the class A plexins in class 3 semaphorin signal transduction, and to clarify the possible role of plexin-A2 in Sema3A signaling.
Mon Dec 15 00:00:00 UTC 2014
NLRP7 affects trophoblast lineage differentiation, binds to overexpressed YY1 and alters CpG methylation.
Human molecular genetics
Mahadevan S,Wen S,Wan YW,Peng HH,Otta S,Liu Z,Iacovino M,Mahen EM,Kyba M,Sadikovic B,Van den Veyver IB
R960-25 was used in Chromatin immunoprecipitation to study how NLRP7 appears to function in chromatin reprogramming and DNA methylation in the germline or early embryonic development.
Sat Feb 01 00:00:00 UTC 2014
Direct interaction between the WD40 repeat protein WDR-23 and SKN-1/Nrf inhibits binding to target DNA.
Molecular and cellular biology
Leung CK,Hasegawa K,Wang Y,Deonarine A,Tang L,Miwa J,Choe KP
R960-25 was used in Western Blotting to examine how WDR-23 interacts with and regulates SKN-1 to inhibit binding at target DNA.
Fri Aug 01 00:00:00 UTC 2014
The SAP motif and C-terminal RS- and RD/E-rich region influences the sub-nuclear localization of Acinus isoforms.
Journal of cellular biochemistry
Wang F,Wendling KS,Soprano KJ,Soprano DR
R960-25 was used in Western Blotting to identify the different functional roles of Acinus-L and Acinus-S' by studying the difference in nuclear localization.
Mon Dec 01 00:00:00 UTC 2014
Intracellular trafficking of AIP56, an NF-κB-cleaving toxin from Photobacterium damselae subsp. piscicida.
Infection and immunity
Pereira LM,Pinto RD,Silva DS,Moreira AR,Beitzinger C,Oliveira P,Sampaio P,Benz R,Azevedo JE,dos Santos NM,do Vale A
R960-25 was used in Western Blotting to show that mouse bone marrow-derived macrophages (mBMDM) intoxicated by AIP56 undergo NF-κB p65 depletion and apoptosis.
Mon Dec 01 00:00:00 UTC 2014
SUMV-1 antagonizes the activity of synthetic multivulva genes in Caenorhabditis elegans.
Developmental biology
Yücel D,Hoe M,Llamosas E,Kant S,Jamieson C,Young PA,Crossley M,Nicholas HR
R960-25 was used in Western Blotting to suggest that SUMV-1 and SUMV-2 may function together with MYS-2 in a nematode KAT8/MOF-like complex to antagonise the activity of the synMuv genes.
Fri Aug 15 00:00:00 UTC 2014
PHD3 regulates EGFR internalization and signalling in tumours.
Nature communications
Garvalov BK,Foss F,Henze AT,Bethani I,Gräf-Höchst S,Singh D,Filatova A,Dopeso H,Seidel S,Damm M,Acker-Palmer A,Acker T
R960-25 was used in Western Blotting to reveal that PHD3 inactivation provides a novel route of EGFR activation to sustain proliferative signalling in the hypoxic microenvironment.
Tue Nov 25 00:00:00 UTC 2014
Tristetraprolin is a novel regulator of BDNF.
SpringerPlus
Kumar A,Varendi K,Peränen J,Andressoo JO
R960-25 was used in western blot to report that tristetraprolin is a novel regulator of brain-derived neurotrophic factor
Fri Oct 03 00:00:00 UTC 2014
Identification of HIV-1 Vif regions required for CBF-β interaction and APOBEC3 suppression.
PloS one
Wang H,Liu B,Liu X,Li Z,Yu XF,Zhang W
R960-25 was used in western blot to map regions of HIV-1 Vif that interact with CBF-beta
Fri Oct 16 00:00:00 UTC 2015
Transnitrosylation from DJ-1 to PTEN attenuates neuronal cell death in parkinson's disease models.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Choi MS,Nakamura T,Cho SJ,Han X,Holland EA,Qu J,Petsko GA,Yates JR,Liddington RC,Lipton SA
R960-25 was used in Western Blot to study the inhibition of phosphatase and tensin homolog (PTEN) phosphatase activity, mediated via a transnitrosylation reaction.
Wed Nov 05 00:00:00 UTC 2014
Defining the factors that contribute to on-target specificity of antisense oligonucleotides.
PloS one
Lima WF,Vickers TA,Nichols J,Li C,Crooke ST
R960-25 was used in western blot to investigate factors that modulate the activity and specificity of antisense oligonucleotides
Mon Nov 09 00:00:00 UTC 2015
CDK5-mediated phosphorylation of p19INK4d avoids DNA damage-induced neurodegeneration in mouse hippocampus and prevents loss of cognitive functions.
Biochimica et biophysica acta
Ogara MF,Belluscio LM,de la Fuente V,Berardino BG,Sonzogni SV,Byk L,Marazita M,Cánepa ET
R960-25 was used in Western Blotting to showed that decreased levels of p19INK4d rendered hippocampal neurons more sensitive to genotoxic insult resulting in the loss of cognitive abilities that rely on the integrity of this brain structure.
Tue Jul 01 00:00:00 UTC 2014
Endocytosis of secreted carboxyl ester lipase in a syndrome of diabetes and pancreatic exocrine dysfunction.
The Journal of biological chemistry
Torsvik J,Johansson BB,Dalva M,Marie M,Fjeld K,Johansson S,Bjørkøy G,Saraste J,Njølstad PR,Molven A
R960-25 was used in Western Blotting to investigate whether the disease-causing c.1686delT mutation affects subcellular distribution, intracellular transport, and degradation of the CEL protein in cell line models.
Fri Oct 17 00:00:00 UTC 2014
Human immunodeficiency virus type 1 enhancer-binding protein 3 is essential for the expression of asparagine-linked glycosylation 2 in the regulation of osteoblast and chondrocyte differentiation.
The Journal of biological chemistry
Imamura K,Maeda S,Kawamura I,Matsuyama K,Shinohara N,Yahiro Y,Nagano S,Setoguchi T,Yokouchi M,Ishidou Y,Komiya S
R960-25 was used in western blot to study the role of human immunodeficiency virus type 1 enhancer-binding protein 3 in the expression of asparagine-linked glycosylation 2 in the regulation of osteoblast and chondrocyte differentiation.
Fri Apr 04 00:00:00 UTC 2014
The light-induced FOS response in melanopsin expressing HEK-293 cells is correlated with melanopsin quantity and dependent on light duration and irradiance.
Photochemistry and photobiology
Georg B,Rask L,Hannibal J,Fahrenkrug J
R960-25 was used in Western Blotting to establish a HEK-hMel cell line to reveal dependency of the FOS response on both melanopsin amount and light intensity in addition to a detailed time-course of both FOS mRNA and protein.
Wed Sep 30 00:00:00 UTC 2015
Ligand independence of the T618I mutation in the colony-stimulating factor 3 receptor (CSF3R) protein results from loss of O-linked glycosylation and increased receptor dimerization.
The Journal of biological chemistry
Maxson JE,Luty SB,MacManiman JD,Abel ML,Druker BJ,Tyner JW
R960-25 was used in Western Blotting to show the combination of CSF3R membrane proximal and truncation mutations, causes enhanced cellular transformation when compared with either mutation alone.
Fri Feb 28 00:00:00 UTC 2014
Regulation of age-related structural integrity in neurons by protein with tau-like repeats (PTL-1) is cell autonomous.
Scientific reports
Chew YL,Fan X,Götz J,Nicholas HR
R960-25 was used in immunohistochemistry to report that the regulation of neuronal ageing by PTL-1 occurs via a cell-autonomous mechanism
Thu Jun 05 00:00:00 UTC 2014
HIV-1 Vif N-terminal motif is required for recruitment of Cul5 to suppress APOBEC3.
Retrovirology
Evans SL,Schön A,Gao Q,Han X,Zhou X,Freire E,Yu XF
R960-25 was used in western blot to identify core binding factor beta as a member of the E3 ubiquitin ligase complex.
Tue Jan 14 00:00:00 UTC 2014
Heat shock protein 83 (Hsp83) facilitates methoprene-tolerant (Met) nuclear import to modulate juvenile hormone signaling.
The Journal of biological chemistry
He Q,Wen D,Jia Q,Cui C,Wang J,Palli SR,Li S
R960-25 was used in Immunohistochemistry to show Hsp83 facilitates the juvenile hormone-induced nuclear import of Met through the juvenile hormone response region, inducing Kr-h1 expression.
Fri Oct 03 00:00:00 UTC 2014
Lysine-specific demethylase 1 regulates differentiation onset and migration of trophoblast stem cells.
Nature communications
Zhu D,Hölz S,Metzger E,Pavlovic M,Jandausch A,Jilg C,Galgoczy P,Herz C,Moser M,Metzger D,Günther T,Arnold SJ,Schüle R
R960-25 was used in Western Blotting to investigate whether lysine-specific demethylase 1 directly regulates Ovol2 expression in trophoblast stem cells in mice.
Mon Mar 07 00:00:00 UTC 2016
Isolation of small interfering RNAs using viral suppressors of RNA interference.
Methods in molecular biology (Clifton, N.J.)
van den Beek M,Antoniewski C,Carré C
R960-25 was used in Immunoprecipitation to detail a protocol for the immunoprecipitation of siRNAs bound to epitope-tagged VSR and subsequent analysis by 3'-end-labeling using cytidine-3',5'-bis phosphate ([5'-(32)P]pCp) and northern blotting.
Thu Jan 22 00:00:00 UTC 2015
The protein synthesis inhibitor blasticidin s enters mammalian cells via leucine-rich repeat-containing protein 8D.
The Journal of biological chemistry
Lee CC,Freinkman E,Sabatini DM,Ploegh HL
R960-25 was used in Immunoprecipitation to identify a role for leucine-rich repeat-containing 8 (LRRC8) proteins, which by forming one or more complexes mediate cell-cell communication by transporting small solutes.
Fri Jun 13 00:00:00 UTC 2014
A direct interaction between the sigma-1 receptor and the hERG voltage-gated K+ channel revealed by atomic force microscopy and homogeneous time-resolved fluorescence (HTRF®).
The Journal of biological chemistry
Balasuriya D,D'Sa L,Talker R,Dupuis E,Maurin F,Martin P,Borgese F,Soriani O,Edwardson JM
R960-25 was used in Immuno-assay to investigate the nature of the interaction between the sigma-1 receptor and hERG.
Fri Nov 14 00:00:00 UTC 2014
Sequestration of latent TGF-β binding protein 1 into CADASIL-related Notch3-ECD deposits.
Acta neuropathologica communications
Kast J,Hanecker P,Beaufort N,Giese A,Joutel A,Dichgans M,Opherk C,Haffner C
R960-25 was used in Western Blot to study three extracellular matrix constituents involved in the regulation of transforming growth factor-Β bioavailability, in post-mortem brain tissue from CADASIL patients and control subjects.
Wed Aug 13 00:00:00 UTC 2014
Importance of extended protease substrate recognition motifs in steering BNIP-2 cleavage by human and mouse granzymes B.
BMC biochemistry
Van Damme P,Plasman K,Vandemoortele G,Jonckheere V,Maurer-Stroh S,Gevaert K
R960-25 was used in Immunoprecipitation to show that the substrate sequence context beyond the P4-P1 positions can influence orthologous granzyme B cleavage efficiencies to an unmatched extent.
Wed Sep 10 00:00:00 UTC 2014
Near-genomewide RNAi screening for regulators of BRAF(V600E) -induced senescence identifies RASEF, a gene epigenetically silenced in melanoma.
Pigment cell & melanoma research
Kaplon J,Hömig-Hölzel C,Gao L,Meissl K,Verdegaal EM,van der Burg SH,van Doorn R,Peeper DS
R960-25 was used in Western Blotting to illustrate the power of short hairpin (sh)RNA oncogene-induced senescence bypass screens and identify a potential novel melanoma suppressor gene.
Tue Jul 01 00:00:00 UTC 2014
Protein interaction discovery using parallel analysis of translated ORFs (PLATO).
Nature biotechnology
Zhu J,Larman HB,Gao G,Somwar R,Zhang Z,Laserson U,Ciccia A,Pavlova N,Church G,Zhang W,Kesari S,Elledge SJ
R960-25 was used in Western Blotting to develop an in vitro method for mapping physical interactions between proteins and other molecules.
Mon Apr 01 00:00:00 UTC 2013
Interplay of LRRK2 with chaperone-mediated autophagy.
Nature neuroscience
Orenstein SJ,Kuo SH,Tasset I,Arias E,Koga H,Fernandez-Carasa I,Cortes E,Honig LS,Dauer W,Consiglio A,Raya A,Sulzer D,Cuervo AM
R960-25 was used in Immunohistochemistry-immunofluorescence to study the interplay of leucine-rich repeat kinase 2 with chaperone-mediated autophagy.
Mon Apr 01 00:00:00 UTC 2013
A multi-parametric flow cytometric assay to analyze DNA-protein interactions.
Nucleic acids research
Arbab M,Mahony S,Cho H,Chick JM,Rolfe PA,van Hoff JP,Morris VW,Gygi SP,Maas RL,Gifford DK,Sherwood RI
R960-25 was used in flow cytometry to develop a flow cytometry-based method to study interactions between DNA and transcription factors
Tue Jan 01 00:00:00 UTC 2013
Synthesis of a probe for monitoring HSV1-tk reporter gene expression using chemical exchange saturation transfer MRI.
Nature protocols
Bar-Shir A,Liu G,Greenberg MM,Bulte JW,Gilad AA
R960-25 was used in Immunohistochemistry to show the synthesis of 5-MDHT can be completed within five days, and is stable for more than one year.
Sun Dec 01 00:00:00 UTC 2013
Albumin is recycled from the primary urine by tubular transcytosis.
Journal of the American Society of Nephrology : JASN
Tenten V,Menzel S,Kunter U,Sicking EM,van Roeyen CR,Sanden SK,Kaldenbach M,Boor P,Fuss A,Uhlig S,Lanzmich R,Willemsen B,Dijkman H,Grepl M,Wild K,Kriz W,Smeets B,Floege J,Moeller MJ
R960-25 was used in Immunohistochemistry to provide evidence of a transcytosis within the kidney tubular system that protects albumin and IgG from lysosomal degradation.
Sun Dec 01 00:00:00 UTC 2013
Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I interferon signaling.
PLoS pathogens
Morrison J,Laurent-Rolle M,Maestre AM,Rajsbaum R,Pisanelli G,Simon V,Mulder LC,Fernandez-Sesma A,García-Sastre A
R960-25 was used in western blot to study NS5-mediated STAT2 degradation in dengue virus replication and report that UBR4 inhibits IFN-I signaling via STAT2 degradation
Fri Mar 01 00:00:00 UTC 2013
CD34⁺/CD38⁻ acute myelogenous leukemia cells aberrantly express CD82 which regulates adhesion and survival of leukemia stem cells.
International journal of cancer
Nishioka C,Ikezoe T,Furihata M,Yang J,Serada S,Naka T,Nobumoto A,Kataoka S,Tsuda M,Udaka K,Yokoyama A
R960-25 was used in Flow cytometry/Cell sorting to identify that aberrant CD82 expression may play a role in survival and adhesion of leukemia stem cells to bone marrow microenvironment.
Wed May 01 00:00:00 UTC 2013
Replication protein a links cell cycle progression and the onset of neurogenesis in Drosophila optic lobe development.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Zhou L,Luo H
R960-25 was used in immunohistochemistry to study cell fate in the Drosophila optic lobe.
Wed Feb 13 00:00:00 UTC 2013
Merkel cell polyomavirus large T antigen has growth-promoting and inhibitory activities.
Journal of virology
Cheng J,Rozenblatt-Rosen O,Paulson KG,Nghiem P,DeCaprio JA
R960-25 was used in Western Blotting to identify V5 epitope tagged SV40 and MCPyV large T antigens, and MCPyV early region expression plasmids, to investigate the role of large T antigens of the integrated viral DNA, in Merkel cell carcinoma (MCC).
Sat Jun 01 00:00:00 UTC 2013
A murine Zic3 transcript with a premature termination codon evades nonsense-mediated decay during axis formation.
Disease models & mechanisms
Ahmed JN,Ali RG,Warr N,Wilson HM,Bellchambers HM,Barratt KS,Thompson AJ,Arkell RM
R960-25 was used in immunocytochemistry and western blot to study the contribution of nonsense-mediated decay to mutated ZIC3 transcripts
Wed May 01 00:00:00 UTC 2013
Regulation of PDGFC signalling and extracellular matrix composition by FREM1 in mice.
Disease models & mechanisms
Wiradjaja F,Cottle DL,Jones L,Smyth I
R960-25 was used in immunoprecipitation to investigate the role of FREM1 in mice and their regulation of PDGFC signaling and extracellular matrix composition
Fri Nov 01 00:00:00 UTC 2013
Loss of DJ-1 protein stability and cytoprotective function by Parkinson's disease-associated proline-158 deletion.
Journal of neurochemistry
Rannikko EH,Vesterager LB,Shaik JH,Weber SS,Cornejo Castro EM,Fog K,Jensen PH,Kahle PJ
R960-25 was used in Western Blotting to demonstrate that the P158Δ point mutation may contribute to neurodegeneration by protein destabilisation and hence loss of DJ-1 function.
Mon Apr 01 00:00:00 UTC 2013
New role of signal peptide peptidase to liberate C-terminal peptides for MHC class I presentation.
Journal of immunology (Baltimore, Md. : 1950)
Oliveira CC,Querido B,Sluijter M,de Groot AF,van der Zee R,Rabelink MJ,Hoeben RC,Ossendorp F,van der Burg SH,van Hall T
R960-25 was used in immunocytochemistry and western blot to study the involvement of signal peptide peptidase in MHC class I presentation.
Tue Oct 15 00:00:00 UTC 2013
Regulation of dipeptidyl peptidase 8 and 9 expression in activated lymphocytes and injured liver.
World journal of gastroenterology
Chowdhury S,Chen Y,Yao TW,Ajami K,Wang XM,Popov Y,Schuppan D,Bertolino P,McCaughan GW,Yu DM,Gorrell MD
R960-25 was used in Flow cytometry/Cell sorting to point to biological roles for DPP8 and DPP9 in lymphocyte activation and apoptosis and in hepatocytes during liver disease pathogenesis.
Tue May 21 00:00:00 UTC 2013
Regulation of dipeptidyl peptidase 8 and 9 expression in activated lymphocytes and injured liver.
World journal of gastroenterology
Chowdhury S,Chen Y,Yao TW,Ajami K,Wang XM,Popov Y,Schuppan D,Bertolino P,McCaughan GW,Yu DM,Gorrell MD
R960-25 was used in Flow cytometry/Cell sorting to point to biological roles for DPP8 and DPP9 in lymphocyte activation and apoptosis and in hepatocytes during liver disease pathogenesis.
Tue May 21 00:00:00 UTC 2013
PTL-1 regulates neuronal integrity and lifespan in C. elegans.
Journal of cell science
Chew YL,Fan X,Götz J,Nicholas HR
R960-25 was used in Immunofluorescence to suggest that some of the effects of tau pathology result from the loss of physiological tau function and not solely from a toxic gain-of-function due to accumulation of tau.
Wed May 01 00:00:00 UTC 2013
Dynamic trafficking of STAT5 depends on an unconventional nuclear localization signal.
Journal of cell science
Shin HY,Reich NC
R960-25 was used in Immunoprecipitation to provide insight into the fundamental mechanisms that regulate STAT5 nuclear trafficking and cooperation with the glucocorticoid receptor.
Thu Aug 01 00:00:00 UTC 2013
Cyclin E2 induces genomic instability by mechanisms distinct from cyclin E1.
Cell cycle (Georgetown, Tex.)
Caldon CE,Sergio CM,Burgess A,Deans AJ,Sutherland RL,Musgrove EA
R960-25 was used in western blot to investigate the effect of cyclin E1 and E2 on genomic instability.
Fri Feb 15 00:00:00 UTC 2013
The SNM1B/APOLLO DNA nuclease functions in resolution of replication stress and maintenance of common fragile site stability.
Human molecular genetics
Mason JM,Das I,Arlt M,Patel N,Kraftson S,Glover TW,Sekiguchi JM
R960-25 was used in Flow cytometry/Cell sorting to provide evidence that SNM1B functions in resolving replication stress and preventing accumulation of genomic damage.
Sun Dec 15 00:00:00 UTC 2013
The SNM1B/APOLLO DNA nuclease functions in resolution of replication stress and maintenance of common fragile site stability.
Human molecular genetics
Mason JM,Das I,Arlt M,Patel N,Kraftson S,Glover TW,Sekiguchi JM
R960-25 was used in Flow cytometry/Cell sorting to provide evidence that SNM1B functions in resolving replication stress and preventing accumulation of genomic damage.
Sun Dec 15 00:00:00 UTC 2013
IDOL stimulates clathrin-independent endocytosis and multivesicular body-mediated lysosomal degradation of the low-density lipoprotein receptor.
Molecular and cellular biology
Scotti E,Calamai M,Goulbourne CN,Zhang L,Hong C,Lin RR,Choi J,Pilch PF,Fong LG,Zou P,Ting AY,Pavone FS,Young SG,Tontonoz P
R960-25 was used in western blot to investigate how lipoprotein receptor expression and cellular lipid uptake are regulated.
Mon Apr 01 00:00:00 UTC 2013
HIV-1 Env gp120 structural determinants for peptide triazole dual receptor site antagonism.
Proteins
Tuzer F,Madani N,Kamanna K,Zentner I,LaLonde J,Holmes A,Upton E,Rajagopal S,McFadden K,Contarino M,Sodroski J,Chaiken I
R960-25 was used in Binding experiments to study the importance of gp120 in binding to and inhibition by the peptide triazole, KR21.
Fri Feb 01 00:00:00 UTC 2013
Diacylglycerol lipaseα (DAGLα) and DAGLβ cooperatively regulate the production of 2-arachidonoyl glycerol in autaptic hippocampal neurons.
Molecular pharmacology
Jain T,Wager-Miller J,Mackie K,Straiker A
R960-25 was used in Western Blotting to provide explicit evidence for a role of DAGLΒ in modulating neurotransmission.
Thu Aug 01 00:00:00 UTC 2013
Ubiquilin-1 modulates γ-secretase-mediated ε-site cleavage in neuronal cells.
Biochemistry
Viswanathan J,Haapasalo A,Kurkinen KM,Natunen T,Mäkinen P,Bertram L,Soininen H,Tanzi RE,Hiltunen M
R960-25 was used in western blot to study the ability of a variant of ubiquilin- to increase gamma-secretase-mediated cleavage of APP at the epsilon site.
Tue Jun 04 00:00:00 UTC 2013
Pax3 synergizes with Gli2 and Zic1 in transactivating the Myf5 epaxial somite enhancer.
Developmental biology
Himeda CL,Barro MV,Emerson CP
R960-25 was used in Chromatin immunoprecipitation to indicate that DNA-bound Pax3 strengthens the ability of both Zic1 and Gli2 to transactivate Myf5 in the epaxial somite.
Fri Nov 01 00:00:00 UTC 2013
Par-complex aPKC and Par3 cross-talk with innate immunity NF-κB pathway in epithelial cells.
Biology open
Forteza R,Wald FA,Mashukova A,Kozhekbaeva Z,Salas PJ
R960-25 was used in immunocytochemistry and western blot to investigate the contributions of PKC and Par3 to inflammatory responses
Mon Nov 18 00:00:00 UTC 2013
MTA1 and MTA3 Regulate HIF1a Expression in Hypoxia-Treated Human Trophoblast Cell Line HTR8/Svneo.
Medical journal of obstetrics and gynecology
Wang K,Chen Y,Ferguson SD,Leach RE
R960-25 was used in Western Blotting to investigate the induction of metastasis associated proteins (MTAs) 1 and 3 by hypoxia, where they upregulate HIF1a expression in trophoblasts.
Thu Dec 12 00:00:00 UTC 2013
In silico screening for palmitoyl substrates reveals a role for DHHC1/3/10 (zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting to Rab5-positive endosomes.
The Journal of biological chemistry
Oku S,Takahashi N,Fukata Y,Fukata M
R960-25 was used in Immunocytochemistry-immunoflourescence to demonstrate that neurochondrin and the DHHC1/10 (z1/11) and DHHC3/7 subfamilies are novel palmitoyl substrate-enzyme pairs and that neurochondrin palmitoylation plays an essential role in its specific endosomal targeting.
Fri Jul 05 00:00:00 UTC 2013
Ubiquitin regulates caspase recruitment domain-mediated signaling by nucleotide-binding oligomerization domain-containing proteins NOD1 and NOD2.
The Journal of biological chemistry
Ver Heul AM,Fowler CA,Ramaswamy S,Piper RC
R960-25 was used in Western Blotting to suggest that Ub binding provides a negative feedback loop upon NOD-dependent activation of RIP2.
Fri Mar 08 00:00:00 UTC 2013
Mapping cytoskeletal protein function in cells by means of nanobodies.
Cytoskeleton (Hoboken, N.J.)
Van Audenhove I,Van Impe K,Ruano-Gallego D,De Clercq S,De Muynck K,Vanloo B,Verstraete H,Fernández LÁ,Gettemans J
R960-25 was used in GST Pull Down to summarize a general protocol for the generation, cloning and production of nanobodies.
Tue Oct 01 00:00:00 UTC 2013
Accumulation of a repulsive axonal guidance molecule RGMa in amyloid plaques: a possible hallmark of regenerative failure in Alzheimer's disease brains.
Neuropathology and applied neurobiology
Satoh J,Tabunoki H,Ishida T,Saito Y,Arima K
R960-25 was used in Western Blotting to demonstrate RGMa protein interacts with C-terminal fragment β of amyloid beta precursor protein and amyloid plaques and to show that RGM, produced by TGFβ-activated astrocytes and accumulated in amyloid plaques and the glial scar, could contribute to the regenerative failure of degenerating axons in Alzheimer's disease brains.
Fri Feb 01 00:00:00 UTC 2013
Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle.
The Journal of biological chemistry
Horiuchi K,Kawamura T,Iwanari H,Ohashi R,Naito M,Kodama T,Hamakubo T
R960-25 was used in Western Blotting to show that the WTAP complex is a component of RNA processing machinery important in posttranscriptional control and cell cycle regulation.
Fri Nov 15 00:00:00 UTC 2013
Lysyl oxidase is downregulated by the EWS/FLI1 oncoprotein and its propeptide domain displays tumor supressor activities in Ewing sarcoma cells.
PloS one
Agra N,Cidre F,García-García L,de la Parra J,Alonso J
R960-25 was used in immunoprecipitation and western blot to study the role of lysyl oxidase in Ewing sarcoma
Mon Sep 29 00:00:00 UTC 2014
In vivo cross-linking reveals principally oligomeric forms of α-synuclein and β-synuclein in neurons and non-neural cells.
The Journal of biological chemistry
Dettmer U,Newman AJ,Luth ES,Bartels T,Selkoe D
R960-25 was used in western blot to study the existance of alpha- and beta-synuclein predomonantly as oligomers in normal neuronal and non-neuronal cells.
Fri Mar 01 00:00:00 UTC 2013
The deubiquitinase USP9X suppresses pancreatic ductal adenocarcinoma.
Nature
Pérez-Mancera PA,Rust AG,van der Weyden L,Kristiansen G,Li A,Sarver AL,Silverstein KA,Grützmann R,Aust D,Rümmele P,Knösel T,Herd C,Stemple DL,Kettleborough R,Brosnan JA,Li A,Morgan R,Knight S,Yu J,Stegeman S,Collier LS,ten Hoeve JJ,de Ridder J,Klein AP,Goggins M,Hruban RH,Chang DK,Biankin AV,Grimmond SM,Wessels LF,Wood SA,Iacobuzio-Donahue CA,Pilarsky C,Largaespada DA,Adams DJ,Tuveson DA
R960-25 was used in western blot to identify genes that cooperate with oncogenic Kras(G12D) to accelerate tumorigenesis and promote progression
Sun Apr 29 00:00:00 UTC 2012
Epigenetic repression of cardiac progenitor gene expression by Ezh2 is required for postnatal cardiac homeostasis.
Nature genetics
Delgado-Olguín P,Huang Y,Li X,Christodoulou D,Seidman CE,Seidman JG,Tarakhovsky A,Bruneau BG
R960-25 was used in immunohistochemistry to study the role of Ezh2 during postnatal cardiac homeostasis.
Sun Jan 22 00:00:00 UTC 2012
BAZF, a novel component of cullin3-based E3 ligase complex, mediates VEGFR and Notch cross-signaling in angiogenesis.
Blood
Ohnuki H,Inoue H,Takemori N,Nakayama H,Sakaue T,Fukuda S,Miwa D,Nishiwaki E,Hatano M,Tokuhisa T,Endo Y,Nose M,Higashiyama S
R960-25 was used in Western Blotting to propose that BAZF supports angiogenic sprouting via BAZF-CUL3-based polyubiquitination-dependent degradation of CBF1 to down-regulate Notch signaling.
Thu Mar 15 00:00:00 UTC 2012
MYST protein acetyltransferase activity requires active site lysine autoacetylation.
The EMBO journal
Yuan H,Rossetto D,Mellert H,Dang W,Srinivasan M,Johnson J,Hodawadekar S,Ding EC,Speicher K,Abshiru N,Perry R,Wu J,Yang C,Zheng YG,Speicher DW,Thibault P,Verreault A,Johnson FB,Berger SL,Sternglanz R,McMahon SB,Côté J,Marmorstein R
R960-25 was used in Immunofluorescence to provide an avenue for the autoposttranslational regulation of MYST proteins that is distinct from other acetyltransferases but draws similarities to the phosphoregulation of protein kinases.
Wed Jan 04 00:00:00 UTC 2012
Xenopus Shugoshin 2 regulates the spindle assembly pathway mediated by the chromosomal passenger complex.
The EMBO journal
Rivera T,Ghenoiu C,Rodríguez-Corsino M,Mochida S,Funabiki H,Losada A
R960-25 was used in Western Blotting to suggest that this functional specificity could rely on the association of XSgo1 and XSgo2 with different regulatory subunits of the PP2A complex.
Wed Mar 21 00:00:00 UTC 2012
Highly specific targeting of the TMPRSS2/ERG fusion gene using liposomal nanovectors.
Clinical cancer research : an official journal of the American Association for Cancer Research
Shao L,Tekedereli I,Wang J,Yuca E,Tsang S,Sood A,Lopez-Berestein G,Ozpolat B,Ittmann M
R960-25 was used in Western Blotting to show that argeting the T/E fusion junction in vivo with specific siRNAs delivered via liposomal nanovectors is a promising therapy for men with prostate cancer.
Sat Dec 15 00:00:00 UTC 2012
Vid30 is required for the association of Vid vesicles and actin patches in the vacuole import and degradation pathway.
Autophagy
Alibhoy AA,Giardina BJ,Dunton DD,Chiang HL
R960-25 was used in Western Blotting to study the critical role of VID30 in the association of Vid vesicles and actin patches.
Sun Jan 01 00:00:00 UTC 2012
Cutaneous β-human papillomavirus E6 proteins bind Mastermind-like coactivators and repress Notch signaling.
Proceedings of the National Academy of Sciences of the United States of America
Tan MJ,White EA,Sowa ME,Harper JW,Aster JC,Howley PM
R960-25 was used in Western Blotting to study the effects of bovine papillomavirus type 1 and β-genus human papillomavirus E6 on notch transcriptional activation.
Tue Jun 05 00:00:00 UTC 2012
Nonsense mutations in AAGAB cause punctate palmoplantar keratoderma type Buschke-Fischer-Brauer.
American journal of human genetics
Giehl KA,Eckstein GN,Pasternack SM,Praetzel-Wunder S,Ruzicka T,Lichtner P,Seidl K,Rogers M,Graf E,Langbein L,Braun-Falco M,Betz RC,Strom TM
R960-25 was used in Western Blotting to define the genetic basis of PPKP1 and provide evidence that AAGAB plays an important role in skin integrity.
Fri Oct 05 00:00:00 UTC 2012
Exons 5-15 of kazrin are dispensable for murine epidermal morphogenesis and homeostasis.
The Journal of investigative dermatology
Chhatriwala MK,Cipolat S,Sevilla LM,Nachat R,Watt FM
R960-25 was used in immunocytochemistry to examine kazrin function in vivo
Wed Aug 01 00:00:00 UTC 2012
PIT1 upregulation by HMGA proteins has a role in pituitary tumorigenesis.
Endocrine-related cancer
Palmieri D,Valentino T,De Martino I,Esposito F,Cappabianca P,Wierinckx A,Vitiello M,Lombardi G,Colao A,Trouillas J,Pierantoni GM,Fusco A,Fedele M
R960-25 was used in Western blotting following immunoprecipitation to identify the role of Pit1 overexpression in pituitary tumour development induced by HMGA1B and HMGA2.
Sun Apr 01 00:00:00 UTC 2012
PIT1 upregulation by HMGA proteins has a role in pituitary tumorigenesis.
Endocrine-related cancer
Palmieri D,Valentino T,De Martino I,Esposito F,Cappabianca P,Wierinckx A,Vitiello M,Lombardi G,Colao A,Trouillas J,Pierantoni GM,Fusco A,Fedele M
R960-25 was used in Western blotting following immunoprecipitation to identify the role of Pit1 overexpression in pituitary tumour development induced by HMGA1B and HMGA2.
Sun Apr 01 00:00:00 UTC 2012
RIN1 orchestrates the activation of RAB5 GTPases and ABL tyrosine kinases to determine the fate of EGFR.
Journal of cell science
Balaji K,Mooser C,Janson CM,Bliss JM,Hojjat H,Colicelli J
R960-25 was used in western blot to elucidate factors that regulate epidermal growth factor receptor recycling.
Sat Dec 01 00:00:00 UTC 2012
Mutagenesis of the palmitoylation site in vaccinia virus envelope glycoprotein B5.
The Journal of general virology
Lorenzo MM,Sánchez-Puig JM,Blasco R
R960-25 was used in Immunocytochemistry to study the role of palmitoylation in the biogenesis of the enveloped vaccinia virus.
Sun Apr 01 00:00:00 UTC 2012
LRRK2 Parkinson disease mutations enhance its microtubule association.
Human molecular genetics
Kett LR,Boassa D,Ho CC,Rideout HJ,Hu J,Terada M,Ellisman M,Dauer WT
R960-25 was used in Western Blotting to identify a novel effect of leucine-rich repeat kinase 2 (LRRK2) Parkinson's disease mutations, and highlight a potential role for microtubules in the pathogenesis of LRRK2-related neurodegeneration.
Wed Feb 15 00:00:00 UTC 2012
RMI1 promotes DNA replication fork progression and recovery from replication fork stress.
Molecular and cellular biology
Yang J,O'Donnell L,Durocher D,Brown GW
R960-25 was used in Immunoprecipitation to reveal direct roles of RMI1 in DNA replication and the replication stress response.
Wed Aug 01 00:00:00 UTC 2012
RMI1 promotes DNA replication fork progression and recovery from replication fork stress.
Molecular and cellular biology
Yang J,O'Donnell L,Durocher D,Brown GW
R960-25 was used in Immunoprecipitation to reveal direct roles of RMI1 in DNA replication and the replication stress response.
Wed Aug 01 00:00:00 UTC 2012
Protein refolding in peroxisomes is dependent upon an HSF1-regulated function.
Cell stress & chaperones
Heldens L,van Genesen ST,Hanssen LL,Hageman J,Kampinga HH,Lubsen NH
R960-25 was used in western blot to investigate proteins that regulate the post-heat shock refolding of peroxisomal luciferase
Sat Sep 01 00:00:00 UTC 2012
Blimp1 activation by AP-1 in human lung cancer cells promotes a migratory phenotype and is inhibited by the lysyl oxidase propeptide.
PloS one
Yu Z,Sato S,Trackman PC,Kirsch KH,Sonenshein GE
R960-25 was used in western blot to measure Blimp-1 expression in epithelial-derived tumors
Mon Aug 13 00:00:00 UTC 2012
Nuclear envelope phosphatase 1-regulatory subunit 1 (formerly TMEM188) is the metazoan Spo7p ortholog and functions in the lipin activation pathway.
The Journal of biological chemistry
Han S,Bahmanyar S,Zhang P,Grishin N,Oegema K,Crooke R,Graham M,Reue K,Dixon JE,Goodman JM
R960-25 was used in Western Blotting to investigate how NEP1-R1 is functionally conserved from yeast to humans and functions in the lipin activation pathway.
Fri Jan 27 00:00:00 UTC 2012
CDK2 and PKA mediated-sequential phosphorylation is critical for p19INK4d function in the DNA damage response.
PloS one
Marazita MC,Ogara MF,Sonzogni SV,Martí M,Dusetti NJ,Pignataro OP,Cánepa ET
R960-25 was used in immunoprecipitation to elucidate the activation mechanism of p19INK4d in response to DNA damage
Thu Sep 13 00:00:00 UTC 2012
The M2 module of the Cys-His-rich domain (CHRD) of PCSK9 protein is needed for the extracellular low-density lipoprotein receptor (LDLR) degradation pathway.
The Journal of biological chemistry
Saavedra YG,Day R,Seidah NG
R960-25 was used in an ELISA assay to study how although M2 is dispensable for secretion, its presence is required for the extracellular activity of PCSK9 on cell surface LDLR.
Fri Dec 21 00:00:00 UTC 2012
High-content analysis of proapoptotic EphA4 dependence receptor functions using small-molecule libraries.
Journal of biomolecular screening
Nelersa CM,Barreras H,Runko E,Ricard J,Shi Y,Glass SJ,Bixby JL,Lemmon VP,Liebl DJ
R960-25 was used in Western Blotting to develop a high-content method for screening dependence receptor-signalling pathways, and demonstrate that several candidate small-molecule compounds can inhibit EphA4-mediated cell death.
Sun Jul 01 00:00:00 UTC 2012
Requirements for stress granule recruitment of fused in sarcoma (FUS) and TAR DNA-binding protein of 43 kDa (TDP-43).
The Journal of biological chemistry
Bentmann E,Neumann M,Tahirovic S,Rodde R,Dormann D,Haass C
R960-25 was used in Immunocytochemistry to identify and outline the requirements for FUS and TDP-43 recruitment into stress granules.
Fri Jun 29 00:00:00 UTC 2012
Requirements for stress granule recruitment of fused in sarcoma (FUS) and TAR DNA-binding protein of 43 kDa (TDP-43).
The Journal of biological chemistry
Bentmann E,Neumann M,Tahirovic S,Rodde R,Dormann D,Haass C
R960-25 was used in Immunocytochemistry to identify and outline the requirements for FUS and TDP-43 recruitment into stress granules.
Fri Jun 29 00:00:00 UTC 2012
Reduced APOBEC3H variant anti-viral activities are associated with altered RNA binding activities.
PloS one
Zhen A,Du J,Zhou X,Xiong Y,Yu XF
R960-25 was used in western blot to study conserved features of human APOBEC3 cytidine deaminases
Thu Apr 11 00:00:00 UTC 2013
Frank-ter Haar syndrome protein Tks4 regulates epidermal growth factor-dependent cell migration.
The Journal of biological chemistry
Bögel G,Gujdár A,Geiszt M,Lányi Á,Fekete A,Sipeki S,Downward J,Buday L
R960-25 was used in Western Blotting to identify a novel role for Tks4 in the epidermal growth factor signalling pathway.
Fri Sep 07 00:00:00 UTC 2012
Stress-inducible caspase substrate TRB3 promotes nuclear translocation of procaspase-3.
PloS one
Shimizu K,Takahama S,Endo Y,Sawasaki T
R960-25 was used in immunocytochemistry and western blot to elucidate how TRB3 contributes to ER stress-induced apoptosis
Tue Apr 23 00:00:00 UTC 2013
Functional analysis of in-frame indel ARID1A mutations reveals new regulatory mechanisms of its tumor suppressor functions.
Neoplasia (New York, N.Y.)
Guan B,Gao M,Wu CH,Wang TL,Shih IeM
R960-25 was used in Western Blotting to delineate a mechanism underlying the regulation of ARID1A subcellular distribution and protein stability.
Mon Oct 01 00:00:00 UTC 2012
In vitro and in vivo antagonism of a G protein-coupled receptor (S1P3) with a novel blocking monoclonal antibody.
PloS one
Harris GL,Creason MB,Brulte GB,Herr DR
R960-25 was used in immunocytochemistry to generate and characterize a monoclonal antibody for S1P(3)
Thu Aug 23 00:00:00 UTC 2012
Identification of ubiquitin-specific protease 9X (USP9X) as a deubiquitinase acting on ubiquitin-peroxin 5 (PEX5) thioester conjugate.
The Journal of biological chemistry
Grou CP,Francisco T,Rodrigues TA,Freitas MO,Pinto MP,Carvalho AF,Domingues P,Wood SA,Rodríguez-Borges JE,Sá-Miranda C,Fransen M,Azevedo JE
R960-25 was used in Western Blotting and Immunoprecipitation to identify the most active deubiquitinase acting on ubiquitinated peroxin 5.
Fri Apr 13 00:00:00 UTC 2012
Identification of ubiquitin-specific protease 9X (USP9X) as a deubiquitinase acting on ubiquitin-peroxin 5 (PEX5) thioester conjugate.
The Journal of biological chemistry
Grou CP,Francisco T,Rodrigues TA,Freitas MO,Pinto MP,Carvalho AF,Domingues P,Wood SA,Rodríguez-Borges JE,Sá-Miranda C,Fransen M,Azevedo JE
R960-25 was used in Western Blotting and Immunoprecipitation to identify the most active deubiquitinase acting on ubiquitinated peroxin 5.
Fri Apr 13 00:00:00 UTC 2012
SMAD4-dependent barrier constrains prostate cancer growth and metastatic progression.
Nature
Ding Z,Wu CJ,Chu GC,Xiao Y,Ho D,Zhang J,Perry SR,Labrot ES,Wu X,Lis R,Hoshida Y,Hiller D,Hu B,Jiang S,Zheng H,Stegh AH,Scott KL,Signoretti S,Bardeesy N,Wang YA,Hill DE,Golub TR,Stampfer MJ,Wong WH,Loda M,Mucci L,Chin L,DePinho RA
R960-25 was used in Western Blotting to establish SMAD4 as a key regulator of prostate cancer progression in mice and humans.
Thu Feb 10 00:00:00 UTC 2011
TRIM79α, an interferon-stimulated gene product, restricts tick-borne encephalitis virus replication by degrading the viral RNA polymerase.
Cell host & microbe
Taylor RT,Lubick KJ,Robertson SJ,Broughton JP,Bloom ME,Bresnahan WA,Best SM
R960-25 was used in Western Blotting to show the specificity of TRIM79α for tick-borne encephalitis virus reveals the innate type I interferon response discriminates between closely related flaviviruses.
Thu Sep 15 00:00:00 UTC 2011
The E3 ligase Itch and deubiquitinase Cyld act together to regulate Tak1 and inflammation.
Nature immunology
Ahmed N,Zeng M,Sinha I,Polin L,Wei WZ,Rathinam C,Flavell R,Massoumi R,Venuprasad K
R960-25 was used in western blot to report an Itch-Cyld-mediated regulatory mechanism in macrophages
Sun Nov 06 00:00:00 UTC 2011
Plexin-A4 promotes tumor progression and tumor angiogenesis by enhancement of VEGF and bFGF signaling.
Blood
Kigel B,Rabinowicz N,Varshavsky A,Kessler O,Neufeld G
R960-25 was used in Western blotting following immunoprecipitation to observe that plexin-A1 and plexin-A4 are required simultaneously for transduction of inhibitory sema3A signals.
Thu Oct 13 00:00:00 UTC 2011
Plexin-A4 promotes tumor progression and tumor angiogenesis by enhancement of VEGF and bFGF signaling.
Blood
Kigel B,Rabinowicz N,Varshavsky A,Kessler O,Neufeld G
R960-25 was used in Western blotting following immunoprecipitation to observe that plexin-A1 and plexin-A4 are required simultaneously for transduction of inhibitory sema3A signals.
Thu Oct 13 00:00:00 UTC 2011
Phosphorylation of Tip60 by GSK-3 determines the induction of PUMA and apoptosis by p53.
Molecular cell
Charvet C,Wissler M,Brauns-Schubert P,Wang SJ,Tang Y,Sigloch FC,Mellert H,Brandenburg M,Lindner SE,Breit B,Green DR,McMahon SB,Borner C,Gu W,Maurer U
R960-25 was used in Western Blotting to suggest that glycogen synthase kinase-3 mediated Tip60S86 phosphorylation provides a link between PI3K signalling and apoptosis induction by p53.
Fri Jun 10 00:00:00 UTC 2011
A genome-wide RNAi screen in mouse embryonic stem cells identifies Mp1 as a key mediator of differentiation.
The Journal of experimental medicine
Westerman BA,Braat AK,Taub N,Potman M,Vissers JH,Blom M,Verhoeven E,Stoop H,Gillis A,Velds A,Nijkamp W,Beijersbergen R,Huber LA,Looijenga LH,van Lohuizen M
R960-25 was used in immunocytochemistry to identify genes needed for the initial differentiation of embryonic stem cells
Mon Dec 19 00:00:00 UTC 2011
The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.
The EMBO journal
Finkbeiner E,Haindl M,Muller S
R960-25 was used in western blot to elucidate the role of SUMO in mammalian ribosome biogenesis.
Wed Mar 16 00:00:00 UTC 2011
FGFR-4 Arg³⁸⁸ enhances prostate cancer progression via extracellular signal-related kinase and serum response factor signaling.
Clinical cancer research : an official journal of the American Association for Cancer Research
Yu W,Feng S,Dakhova O,Creighton CJ,Cai Y,Wang J,Li R,Frolov A,Ayala G,Ittmann M
R960-25 was used in Western Blotting to define a signal transduction pathway downstream of FGFR-4 Arg(388) that acts via extracellular signal-related kinase and serum response factor to promote prostate cancer progression.
Fri Jul 01 00:00:00 UTC 2011
p23H implicated as cis/trans regulator of AlaXp-directed editing for mammalian cell homeostasis.
Proceedings of the National Academy of Sciences of the United States of America
Nawaz MH,Merriman E,Yang XL,Schimmel P
R960-25 was used in Western Blotting to demonstrate the dependence of mammalian cell proteostasis on AlaXp, and implicate p23(H) as a cis- and trans-acting regulator of its activity.
Tue Feb 15 00:00:00 UTC 2011
Mortalin-p53 interaction in cancer cells is stress dependent and constitutes a selective target for cancer therapy.
Cell death and differentiation
Lu WJ,Lee NP,Kaul SC,Lan F,Poon RT,Wadhwa R,Luk JM
R960-25 was used in Immunocytochemistry-immunoflourescence to demonstrate that the mortalin-p53 interaction exists in cancer cells that are either physiologically stressed or treated with stress-inducing chemicals.
Wed Jun 01 00:00:00 UTC 2011
Alternative splicing factor or splicing factor-2 plays a key role in intron retention of the endoglin gene during endothelial senescence.
Aging cell
Blanco FJ,Bernabeu C
R960-25 was used in Western Blotting to study the involvement of alternative splicing factor or splicing factor-2 in the synthesis of endoglin.
Sat Oct 01 00:00:00 UTC 2011
Anti-proliferative and pro-apoptotic actions of a novel human and mouse ovarian tumor-associated gene OTAG-12: downregulation, alternative splicing and drug sensitization.
Oncogene
Chen X,Zhang H,Aravindakshan JP,Gotlieb WH,Sairam MR
R960-25 was used in Western Blotting to identify a novel ovarian tumour associated gene-12, which is progressively downregulated in ovarian tumours and maps to Chromosome 8B3.3.
Thu Jun 23 00:00:00 UTC 2011
Human cytomegalovirus UL97 kinase prevents the deposition of mutant protein aggregates in cellular models of Huntington's disease and ataxia.
Neurobiology of disease
Tower C,Fu L,Gill R,Prichard M,Lesort M,Sztul E
R960-25 was used in Immunocytochemistry-immunoflourescence to identify that UL97 can modulate polyglutamine aggregation, and serve as a tool to probe the cellular mechanisms of the formation of aggregates in polyglutamine disorders.
Sat Jan 01 00:00:00 UTC 2011
Identification of cis-elements and transcription factors regulating neuronal activity-dependent transcription of human BDNF gene.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Pruunsild P,Sepp M,Orav E,Koppel I,Timmusk T
R960-25 was used in Western Blotting to study the regulation of neuronal activity-dependent transcription of human brain-derived neurotrophic factor.
Wed Mar 02 00:00:00 UTC 2011
The C proteins of human parainfluenza virus type 1 limit double-stranded RNA accumulation that would otherwise trigger activation of MDA5 and protein kinase R.
Journal of virology
Boonyaratanakornkit J,Bartlett E,Schomacker H,Surman S,Akira S,Bae YS,Collins P,Murphy B,Schmidt A
R960-25 was used in Western Blotting to study the mechanisms by which human parainfluenza virus type 1 limits the activation of interferon regulatory factor 3, NF-κB, and protein kinase R.
Tue Feb 01 00:00:00 UTC 2011
A novel role of dipeptidyl peptidase 9 in epidermal growth factor signaling.
Molecular cancer research : MCR
Yao TW,Kim WS,Yu DM,Sharbeen G,McCaughan GW,Choi KY,Xia P,Gorrell MD
R960-25 was used in Flow cytometry/Cell sorting to suggest an important signaling role of DPP9 in the regulation of survival and proliferation pathways.
Fri Jul 01 00:00:00 UTC 2011
PKA and Epac synergistically inhibit smooth muscle cell proliferation.
Journal of molecular and cellular cardiology
Hewer RC,Sala-Newby GB,Wu YJ,Newby AC,Bond M
R960-25 was used in western blot to elucidate the role of PKA and Epac signaling on cAMP-induced inhibition of VSMC proliferation
Sat Jan 01 00:00:00 UTC 2011
Dual-color quantum dot detection of a heterotetrameric potassium channel (hKCa3.1).
American journal of physiology. Cell physiology
Waschk DE,Fabian A,Budde T,Schwab A
R960-25 was used in Immunofluorescence to determine the subunit composition of the tetrameric K(+) channel hK(Ca)3.1 in humans.
Fri Apr 01 00:00:00 UTC 2011
Mitogen-activated protein kinases promote WNT/beta-catenin signaling via phosphorylation of LRP6.
Molecular and cellular biology
Červenka I,Wolf J,Mašek J,Krejci P,Wilcox WR,Kozubík A,Schulte G,Gutkind JS,Bryja V
R960-25 was used in Western Blotting to examine the role of LDL-related protein 6 phosphorylation by mitogen activated protein kinases as a point of convergence between Wnt/Β-catenin signalling and mitogenic pathways.
Sat Jan 01 00:00:00 UTC 2011
Assessing ubiquitination of viral proteins: Lessons from flavivirus NS5.
Methods (San Diego, Calif.)
Taylor RT,Best SM
R960-25 was used in Western Blotting to describe a protocol for detection and characterisation of the ubiquitination status of a viral substrate protein.
Sat Oct 01 00:00:00 UTC 2011
Modulation of clock gene expression by the transcriptional coregulator receptor interacting protein 140 (RIP140).
Journal of biological rhythms
Poliandri AH,Gamsby JJ,Christian M,Spinella MJ,Loros JJ,Dunlap JC,Parker MG
R960-25 was used in western blot to report that RIP140 mRNA and protein levels are under circadian regulation
Wed Jun 01 00:00:00 UTC 2011
Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1.
Biochemical and biophysical research communications
Ochiai K,Watanabe M,Ueki H,Huang P,Fujii Y,Nasu Y,Noguchi H,Hirata T,Sakaguchi M,Huh NH,Kashiwakura Y,Kaku H,Kumon H
R960-25 was used in western blot to identify binding partners of REIC/Dkk-3.
Fri Aug 26 00:00:00 UTC 2011
Sphingolipid modulation of angiogenic factor expression in neuroblastoma.
Cancer prevention research (Philadelphia, Pa.)
Li MH,Hla T,Ferrer F
R960-25 was used in Western Blotting to suggest that enhanced formation of sphingolipid mediator S1P in neuroblastoma profoundly influences tumour microenvironment by inducing VEGF expression via S1P receptor-2.
Mon Aug 01 00:00:00 UTC 2011
The long noncoding RNA Six3OS acts in trans to regulate retinal development by modulating Six3 activity.
Neural development
Rapicavoli NA,Poth EM,Zhu H,Blackshaw S
R960-25 was used in RNA immunoprecipitation and western blot to study expression of long non-coding RNAs during embryonic development
Wed Sep 21 00:00:00 UTC 2011
A bacterial protein targets the BAHD1 chromatin complex to stimulate type III interferon response.
Science (New York, N.Y.)
Lebreton A,Lakisic G,Job V,Fritsch L,Tham TN,Camejo A,Matteï PJ,Regnault B,Nahori MA,Cabanes D,Gautreau A,Ait-Si-Ali S,Dessen A,Cossart P,Bierne H
R960-25 was used in Immunocytochemistry-immunofluorescence to find that a secreted listerial virulence factor, LntA, could target the chromatin repressor BAHD1 in the host cell nucleus to activate interferon (IFN)-stimulated genes (ISGs).
Fri Mar 11 00:00:00 UTC 2011
Selective inhibition of microRNA accessibility by RBM38 is required for p53 activity.
Nature communications
Léveillé N,Elkon R,Davalos V,Manoharan V,Hollingworth D,Oude Vrielink J,le Sage C,Melo CA,Horlings HM,Wesseling J,Ule J,Esteller M,Ramos A,Agami R
R960-25 was used in western blot to assess the role of RBM38 in p53 regulation.
Tue Oct 25 00:00:00 UTC 2011
Efficient conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector.
PloS one
Addis RC,Hsu FC,Wright RL,Dichter MA,Coulter DA,Gearhart JD
R960-25 was used in immunocytochemistry to report the reprogramming of astrocytes to dopaminergic neurons
Mon Apr 23 00:00:00 UTC 2012
Fibroblast growth factor receptor 3 (FGFR3) is a strong heat shock protein 90 (Hsp90) client: implications for therapeutic manipulation.
The Journal of biological chemistry
Laederich MB,Degnin CR,Lunstrum GP,Holden P,Horton WA
R960-25 was used in Immunoprecipitation to delineate the relationship of Hsp90 and co-chaperone Cdc37 with fibroblast growth factor receptor (FGFR) 3 and the FGFR family.
Fri Jun 03 00:00:00 UTC 2011
The survival effect of mitochondrial Higd-1a is associated with suppression of cytochrome C release and prevention of caspase activation.
Biochimica et biophysica acta
An HJ,Shin H,Jo SG,Kim YJ,Lee JO,Paik SG,Lee H
R960-25 was used in Western Blotting to elucidate how the expression of Higd-1a is induced by hypoxia, and how it mediates its anti-apoptotic effects.
Thu Dec 01 00:00:00 UTC 2011
Mutations in PINK1 and Parkin impair ubiquitination of Mitofusins in human fibroblasts.
PloS one
Rakovic A,Grünewald A,Kottwitz J,Brüggemann N,Pramstaller PP,Lohmann K,Klein C
R960-25 was used in western blot to examine the impact of mutations in endogenous PINK1 and Parkin on the ubiquitination of mitochondrial fusion and fission factors and the mitochondrial network structure
Tue Mar 08 00:00:00 UTC 2011
In silico and in vitro investigations of the mutability of disease-causing missense mutation sites in spermine synthase.
PloS one
Zhang Z,Norris J,Schwartz C,Alexov E
R960-25 was used in western blot to discuss the consequences of known mutations of spermine synthase
Thu Sep 22 00:00:00 UTC 2011
Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization.
The Journal of biological chemistry
Fuhs SR,Insel PA
R960-25 was used in Western Blotting to characterise the SUMOylation of caveolin-3, and elucidate its importance for the regulation of certain signalling partners.
Fri Apr 29 00:00:00 UTC 2011
Schizosaccharomyces pombe minichromosome maintenance-binding protein (MCM-BP) antagonizes MCM helicase.
The Journal of biological chemistry
Ding L,Forsburg SL
R960-25 was used in Western Blotting to examine the interactions between Mcm-binding protein 1 and the minichromosome maintenance complex helicase.
Fri Sep 23 00:00:00 UTC 2011
Carcinoembryonic antigen interacts with TGF-{beta} receptor and inhibits TGF-{beta} signaling in colorectal cancers.
Cancer research
Li Y,Cao H,Jiao Z,Pakala SB,Sirigiri DN,Li W,Kumar R,Mishra L
R960-25 was used in immunoprecipitation and western blot to report that carcinoembryonic antigen directly interacts with TGF-beta receptor and inhibits TGF-beta signaling
Fri Oct 15 00:00:00 UTC 2010
Activated BRAF induces gliomas in mice when combined with Ink4a/Arf loss or Akt activation.
Oncogene
Robinson JP,VanBrocklin MW,Guilbeault AR,Signorelli DL,Brandner S,Holmen SL
Thu Jan 21 00:00:00 UTC 2010
Disrupted transforming growth factor-beta signaling in spinal and bulbar muscular atrophy.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Katsuno M,Adachi H,Minamiyama M,Waza M,Doi H,Kondo N,Mizoguchi H,Nitta A,Yamada K,Banno H,Suzuki K,Tanaka F,Sobue G
R960-25 was used in Western Blotting to examine whether TGF-beta signalling is dysregulated in spinal bulbar muscular atrophy.
Wed Apr 21 00:00:00 UTC 2010
Z proteins of New World arenaviruses bind RIG-I and interfere with type I interferon induction.
Journal of virology
Fan L,Briese T,Lipkin WI
R960-25 was used in Western Blotting to demonstrate that the Z proteins of four New World arenaviruses bind to retinoic acid-inducible gene I product, resulting in downregulation of the interferon-beta response.
Mon Feb 01 00:00:00 UTC 2010
A single amino acid difference in human APOBEC3H variants determines HIV-1 Vif sensitivity.
Journal of virology
Zhen A,Wang T,Zhao K,Xiong Y,Yu XF
R960-25 was used in Western Blotting to investigate whether HIV-1 Vif can recognise and suppress the antiviral activity of APOBEC 3H variants, as it does with other APOBEC3 proteins.
Mon Feb 01 00:00:00 UTC 2010
Association of potent human antiviral cytidine deaminases with 7SL RNA and viral RNP in HIV-1 virions.
Journal of virology
Zhang W,Du J,Yu K,Wang T,Yong X,Yu XF
R960-25 was used in Western Blotting to study how potent anti-HIV-1 cytidine deaminases have evolved to possess a unique RNA-binding ability for precise HIV-1 targeting and viral inhibition.
Wed Dec 01 00:00:00 UTC 2010
A novel transmembrane domain mediating retention of a highly motile herpesvirus glycoprotein in the endoplasmic reticulum.
The Journal of general virology
Däubner T,Fink A,Seitz A,Tenzer S,Müller J,Strand D,Seckert CK,Janssen C,Renzaho A,Grzimek NK,Simon CO,Ebert S,Reddehase MJ,Oehrlein-Karpi SA,Lemmermann NA
R960-25 was used in Western Blotting to reveal that the m164 gene product shows unusual features in its cell biology.
Tue Jun 01 00:00:00 UTC 2010
The RET51/FKBP52 complex and its involvement in Parkinson disease.
Human molecular genetics
Fusco D,Vargiolu M,Vidone M,Mariani E,Pennisi LF,Bonora E,Capellari S,Dirnberger D,Baumeister R,Martinelli P,Romeo G
R960-25 was used in Immunoprecipitation to find a compound heterozygous carrying two mutations in RET and FKBP52 that are sufficient to disrupt the RET51/FKBP52 complex, indicating its potential role in PD.
Thu Jul 15 00:00:00 UTC 2010
The RET51/FKBP52 complex and its involvement in Parkinson disease.
Human molecular genetics
Fusco D,Vargiolu M,Vidone M,Mariani E,Pennisi LF,Bonora E,Capellari S,Dirnberger D,Baumeister R,Martinelli P,Romeo G
R960-25 was used in Immunoprecipitation to find a compound heterozygous carrying two mutations in RET and FKBP52 that are sufficient to disrupt the RET51/FKBP52 complex, indicating its potential role in PD.
Thu Jul 15 00:00:00 UTC 2010
Mutant huntingtin fragment selectively suppresses Brn-2 POU domain transcription factor to mediate hypothalamic cell dysfunction.
Human molecular genetics
Yamanaka T,Tosaki A,Miyazaki H,Kurosawa M,Furukawa Y,Yamada M,Nukina N
R960-25 was used in Western Blotting to analyse the altered DNA binding of multiple transcription factors using R6/2 Huntington's disease model mice brains that express an N-terminal fragment of mutant huntingtin.
Tue Jun 01 00:00:00 UTC 2010
Effect of GFP tags on the localization of EB1 and EB1 fragments in vivo.
Cytoskeleton (Hoboken, N.J.)
Skube SB,Chaverri JM,Goodson HV
R960-25 was used in Immunofluorescence to survey the localization of expressed EB1 fragments and investigate whether GFP tags alter these localizations.
Fri Jan 01 00:00:00 UTC 2010
Surfactant protein A2 mutations associated with pulmonary fibrosis lead to protein instability and endoplasmic reticulum stress.
The Journal of biological chemistry
Maitra M,Wang Y,Gerard RD,Mendelson CR,Garcia CK
R960-25 was used in Western Blotting to examine the synthesis, secretion, oligomerization, detergent solubility, protein stability and functionality of surfactant protein A2 (SP-A2) variants.
Fri Jul 16 00:00:00 UTC 2010
Mutational analysis of NHAoc/NHA2 in Saccharomyces cerevisiae.
Biochimica et biophysica acta
Huang X,Morse LR,Xu Y,Zahradka J,Sychrová H,Stashenko P,Fan F,Battaglino RA
R960-25 was used in Immunofluorescence to conduct a mutational analysis of the osteoclast-specific Sodium-Proton antiporter NHAoc/NHA2 in yeast.
Wed Dec 01 00:00:00 UTC 2010
CADM1 interacts with Tiam1 and promotes invasive phenotype of human T-cell leukemia virus type I-transformed cells and adult T-cell leukemia cells.
The Journal of biological chemistry
Masuda M,Maruyama T,Ohta T,Ito A,Hayashi T,Tsukasaki K,Kamihira S,Yamaoka S,Hoshino H,Yoshida T,Watanabe T,Stanbridge EJ,Murakami Y
R960-25 was used in Western Blotting to demonstrate that the cytoplasmic domain of CADM1 directly interacts with the PSD95/Dlg/ZO-1 domain of Tiam1, inducing formation of lamellipodia through Rac activation in T cell leukaemia cell lines.
Fri May 14 00:00:00 UTC 2010
O-glycosylation modulates proprotein convertase activation of angiopoietin-like protein 3: possible role of polypeptide GalNAc-transferase-2 in regulation of concentrations of plasma lipids.
The Journal of biological chemistry
Schjoldager KT,Vester-Christensen MB,Bennett EP,Levery SB,Schwientek T,Yin W,Blixt O,Clausen H
R960-25 was used in Western Blotting to demonstrate that ANGPTL3 activation is modulated by O-glycosylation and that this step is likely controlled by GalNAc-T2.
Fri Nov 19 00:00:00 UTC 2010
A sequence variation (I148M) in PNPLA3 associated with nonalcoholic fatty liver disease disrupts triglyceride hydrolysis.
The Journal of biological chemistry
He S,McPhaul C,Li JZ,Garuti R,Kinch L,Grishin NV,Cohen JC,Hobbs HH
R960-25 was used in western blot to assess the effect of an I148M substitution on the enzymatic activity and cellular location of PNPLA3
Fri Feb 26 00:00:00 UTC 2010
DUSP26 negatively affects the proliferation of epithelial cells, an effect not mediated by dephosphorylation of MAPKs.
Biochimica et biophysica acta
Patterson KI,Brummer T,Daly RJ,O'Brien PM
R960-25 was used in Western Blotting to characterise the phosphatase activity of the atypical dual specificity phosphatase, DUSP26, on mitogen-activated protein kinases, and to determine its expression, regulation and function in cancer cells.
Wed Sep 01 00:00:00 UTC 2010
DUSP26 negatively affects the proliferation of epithelial cells, an effect not mediated by dephosphorylation of MAPKs.
Biochimica et biophysica acta
Patterson KI,Brummer T,Daly RJ,O'Brien PM
R960-25 was used in Western Blotting to characterise the phosphatase activity of the atypical dual specificity phosphatase, DUSP26, on mitogen-activated protein kinases, and to determine its expression, regulation and function in cancer cells.
Wed Sep 01 00:00:00 UTC 2010
Molecular consequences of the ACVR1(R206H) mutation of fibrodysplasia ossificans progressiva.
The Journal of biological chemistry
Song GA,Kim HJ,Woo KM,Baek JH,Kim GS,Choi JY,Ryoo HM
R960-25 was used in Immunocytochemistry to study how impaired binding to FKBP1A and an altered subcellular distribution by R206H ACVR1 mutation may result in mild activation of osteogenic BMP-signaling in extraskeletal sites such as muscle.
Fri Jul 16 00:00:00 UTC 2010
Molecular consequences of the ACVR1(R206H) mutation of fibrodysplasia ossificans progressiva.
The Journal of biological chemistry
Song GA,Kim HJ,Woo KM,Baek JH,Kim GS,Choi JY,Ryoo HM
R960-25 was used in Immunocytochemistry to study how impaired binding to FKBP1A and an altered subcellular distribution by R206H ACVR1 mutation may result in mild activation of osteogenic BMP-signaling in extraskeletal sites such as muscle.
Fri Jul 16 00:00:00 UTC 2010
Molecular consequences of the ACVR1(R206H) mutation of fibrodysplasia ossificans progressiva.
The Journal of biological chemistry
Song GA,Kim HJ,Woo KM,Baek JH,Kim GS,Choi JY,Ryoo HM
R960-25 was used in Immunocytochemistry to study how impaired binding to FKBP1A and an altered subcellular distribution by R206H ACVR1 mutation may result in mild activation of osteogenic BMP-signaling in extraskeletal sites such as muscle.
Fri Jul 16 00:00:00 UTC 2010
CCCTC-binding factor and the transcription factor T-bet orchestrate T helper 1 cell-specific structure and function at the interferon-gamma locus.
Immunity
Sekimata M,Pérez-Melgosa M,Miller SA,Weinmann AS,Sabo PJ,Sandstrom R,Dorschner MO,Stamatoyannopoulos JA,Wilson CB
R960-25 was used in Chromatin immunoprecipitation to demonstrate that cooperation between architectural and transcriptional enhancing factors and the elements to which they bind is required for proper Th1 cell-specific expression of Ifng.
Fri Oct 16 00:00:00 UTC 2009
Somatic single hits inactivate the X-linked tumor suppressor FOXP3 in the prostate.
Cancer cell
Wang L,Liu R,Li W,Chen C,Katoh H,Chen GY,McNally B,Lin L,Zhou P,Zuo T,Cooney KA,Liu Y,Zheng P
R960-25 was used in Western Blotting to demonstrate that somatic inactivating mutations and deletion of the X-linked FOXP3 gene residing at Xp11.23 in human prostate cancer.
Tue Oct 06 00:00:00 UTC 2009
CBL exon 8/9 mutants activate the FLT3 pathway and cluster in core binding factor/11q deletion acute myeloid leukemia/myelodysplastic syndrome subtypes.
Clinical cancer research : an official journal of the American Association for Cancer Research
Reindl C,Quentmeier H,Petropoulos K,Greif PA,Benthaus T,Argiropoulos B,Mellert G,Vempati S,Duyster J,Buske C,Bohlander SK,Humphries KR,Hiddemann W,Spiekermann K
R960-25 was used in Immunocytochemistry to determine the frequency of CBL mutations in acute leukaemias and evaluate the oncogenic potential of mutant CBL.
Wed Apr 01 00:00:00 UTC 2009
A large-scale functional RNAi screen reveals a role for CK2 in the mammalian circadian clock.
Genes & development
Maier B,Wendt S,Vanselow JT,Wallach T,Reischl S,Oehmke S,Schlosser A,Kramer A
R960-25 was used in Western Blotting to elucidate the role of casein kinase 2 as a novel component of the mammalian circadian clock.
Sun Mar 15 00:00:00 UTC 2009
A large-scale functional RNAi screen reveals a role for CK2 in the mammalian circadian clock.
Genes & development
Maier B,Wendt S,Vanselow JT,Wallach T,Reischl S,Oehmke S,Schlosser A,Kramer A
R960-25 was used in Western Blotting to elucidate the role of casein kinase 2 as a novel component of the mammalian circadian clock.
Sun Mar 15 00:00:00 UTC 2009
Stat3 activation is required for the growth of U87 cell-derived tumours in mice.
European journal of cancer (Oxford, England : 1990)
Dasgupta A,Raychaudhuri B,Haqqi T,Prayson R,Van Meir EG,Vogelbaum M,Haque SJ
R960-25 was used in Western Blotting to study the contribution of activated Stat3 to the growth of glioblastoma multiforme.
Sun Mar 01 00:00:00 UTC 2009
A patch of positively charged amino acids surrounding the human immunodeficiency virus type 1 Vif SLVx4Yx9Y motif influences its interaction with APOBEC3G.
Journal of virology
Chen G,He Z,Wang T,Xu R,Yu XF
R960-25 was used in Western Blotting to demonstrate that the SLV/Ix4Yx9Y motif and surrounding amino acids represent an important functional domain in the Vif-mediated defence against APOBEC3.
Tue Sep 01 00:00:00 UTC 2009
Requirement of a dynein light chain in TGFbeta/Smad3 signaling.
Journal of cellular physiology
Jin Q,Gao G,Mulder KM
R960-25 was used in Western Blotting to study the functions of km23-2 in TGFbeta signalling.
Tue Dec 01 00:00:00 UTC 2009
CHAC1/MGC4504 is a novel proapoptotic component of the unfolded protein response, downstream of the ATF4-ATF3-CHOP cascade.
Journal of immunology (Baltimore, Md. : 1950)
Mungrue IN,Pagnon J,Kohannim O,Gargalovic PS,Lusis AJ
R960-25 was used in Immunocytochemistry-immunoflourescence to characterise the role of CHAC1/MGC4504 as a proapoptotic component of the unfolded protein response.
Thu Jan 01 00:00:00 UTC 2009
Casein kinase 1 delta regulates the pace of the mammalian circadian clock.
Molecular and cellular biology
Etchegaray JP,Machida KK,Noton E,Constance CM,Dallmann R,Di Napoli MN,DeBruyne JP,Lambert CM,Yu EA,Reppert SM,Weaver DR
R960-25 was used in Immunoprecipitation to reveal important functional difference of CK1delta over CK1epsilon in maintaining the 24-h circadian cycle length in murine model.
Wed Jul 01 00:00:00 UTC 2009
Loss-of-function point mutations and two-furin domain derivatives provide insights about R-spondin2 structure and function.
Cellular signalling
Li SJ,Yen TY,Endo Y,Klauzinska M,Baljinnyam B,Macher B,Callahan R,Rubin JS
R960-25 was used in Immunoprecipitation to analyse the functions and structures of R-spondin variants.
Mon Jun 01 00:00:00 UTC 2009
Loss-of-function point mutations and two-furin domain derivatives provide insights about R-spondin2 structure and function.
Cellular signalling
Li SJ,Yen TY,Endo Y,Klauzinska M,Baljinnyam B,Macher B,Callahan R,Rubin JS
R960-25 was used in Immunoprecipitation to analyse the functions and structures of R-spondin variants.
Mon Jun 01 00:00:00 UTC 2009
A functional heteromeric MIF receptor formed by CD74 and CXCR4.
FEBS letters
Schwartz V,Lue H,Kraemer S,Korbiel J,Krohn R,Ohl K,Bucala R,Weber C,Bernhagen J
R960-25 was used in Western Blotting to demonstrate that CD74/CXCR4 complexes formed when CD74 was expressed with CXCR4 in HEK293 cells.
Thu Sep 03 00:00:00 UTC 2009
TIF1beta/KAP-1 is a coactivator of the orphan nuclear receptor NGFI-B/Nur77.
The Journal of biological chemistry
Rambaud J,Desroches J,Balsalobre A,Drouin J
R960-25 was used in Western Blotting to indicate that TIF1beta is an important coactivator of NGFI-B-dependent transcription.
Fri May 22 00:00:00 UTC 2009
Matrix metalloproteinase 12 silencing: a therapeutic approach to treat pathological lung tissue remodeling?
Pulmonary pharmacology & therapeutics
Garbacki N,Di Valentin E,Piette J,Cataldo D,Crahay C,Colige A
R960-25 was used in Immunohistochemistry, ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot to study MMP-12 structure, functions and mechanisms of activation and regulation, including potential MMP-12 modulation by microRNA.
Sat Aug 01 00:00:00 UTC 2009
Transcriptional interaction of an estrogen receptor splice variant and ErbB4 suggests convergence in gene susceptibility pathways in schizophrenia.
The Journal of biological chemistry
Wong J,Weickert CS
R960-25 was used in Western Blotting to demonstrate convergence between ERalpha and ErbB4-ICD in the transcriptional control of ERalpha-target gene expression and suggest it may be disrupted in schizophrenia.
Fri Jul 10 00:00:00 UTC 2009
Gene-specific transcriptional activation mediated by the p150 subunit of the chromatin assembly factor 1.
The Journal of biological chemistry
Lee SB,Ou DS,Lee CF,Juan LJ
R960-25 was used in Western Blotting to study how p150 directly activates transcription, independently of its histone deposition function.
Fri May 22 00:00:00 UTC 2009
Crude subcellular fractionation of cultured mammalian cell lines.
BMC research notes
Holden P,Horton WA
R960-25 was used in western blot to discuss methods to isolate recombinant proteins from mammalian cells
Thu Dec 10 00:00:00 UTC 2009
Exo70-mediated recruitment of nucleoporin Nup62 at the leading edge of migrating cells is required for cell migration.
Traffic (Copenhagen, Denmark)
Hubert T,Vandekerckhove J,Gettemans J
R960-25 was used in Western Blot to study the interaction between Nup62 and Exo70 at the leading edge of migrating cells.
Tue Sep 01 00:00:00 UTC 2009
Inhibitory activity of myelin-associated glycoprotein on sensory neurons is largely independent of NgR1 and NgR2 and resides within Ig-Like domains 4 and 5.
PloS one
Wörter V,Schweigreiter R,Kinzel B,Mueller M,Barske C,Böck G,Frentzel S,Bandtlow CE
R960-25 was used in immunocytochemistry and western blot to elucidate the contributions of Nogo-receptors and gangliosides to myelin-associated glycoprotein-mediated inhibition of sensory neurons
Thu Jul 30 00:00:00 UTC 2009
SirT1 inhibition reduces IGF-I/IRS-2/Ras/ERK1/2 signaling and protects neurons.
Cell metabolism
Li Y,Xu W,McBurney MW,Longo VD
R960-25 was used in Immunoprecipitation to suggest that SirT1 contributes to oxidative damage in mammals by activating IRS-2/Ras/ERK signaling downstream of insulin/IGF-I receptors.
Tue Jul 01 00:00:00 UTC 2008
p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping.
Neuron
Lim YS,McLaughlin T,Sung TC,Santiago A,Lee KF,O'Leary DD
R960-25 was used in Immunoprecipitation to conclude that p75(NTR) is a signaling partner for ephrin-As and the ephrin-A- p75(NTR) complex reverse signals to mediate axon repulsion required for guidance and mapping.
Thu Sep 11 00:00:00 UTC 2008
p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping.
Neuron
Lim YS,McLaughlin T,Sung TC,Santiago A,Lee KF,O'Leary DD
R960-25 was used in Immunoprecipitation to conclude that p75(NTR) is a signaling partner for ephrin-As and the ephrin-A- p75(NTR) complex reverse signals to mediate axon repulsion required for guidance and mapping.
Thu Sep 11 00:00:00 UTC 2008
Mutant Huntingtin reduces HSP70 expression through the sequestration of NF-Y transcription factor.
The EMBO journal
Yamanaka T,Miyazaki H,Oyama F,Kurosawa M,Washizu C,Doi H,Nukina N
R960-25 was used in Immunocytochemistry-immunoflourescence to study the interaction between mutant Huntingtin and the components of NF-Y transcriptional factor in vitro and in a Huntington's disease model mouse brain.
Wed Mar 19 00:00:00 UTC 2008
The helix-loop-helix protein Id1 requires cyclin D1 to promote the proliferation of mammary epithelial cell acini.
Cancer research
Caldon CE,Swarbrick A,Lee CS,Sutherland RL,Musgrove EA
R960-25 was used in Western Blotting to show that the down-regulation of endogenous Id1 via proteosomal degradation and relocalization from the nucleus to the cytoplasm is an early event in the formation of mammary epithelial acini.
Tue Apr 15 00:00:00 UTC 2008
Protein-sphingolipid interactions within cellular membranes.
Journal of lipid research
Haberkant P,Schmitt O,Contreras FX,Thiele C,Hanada K,Sprong H,Reinhard C,Wieland FT,Brügger B
R960-25 was used in Immunoprecipitation to demonstrate that a novel photolabile sphingolipid probe allows the detection of protein-sphingolipid interactions within the membrane bilayer of living cells.
Tue Jan 01 00:00:00 UTC 2008
NHA-oc/NHA2: a mitochondrial cation-proton antiporter selectively expressed in osteoclasts.
Bone
Battaglino RA,Pham L,Morse LR,Vokes M,Sharma A,Odgren PR,Yang M,Sasaki H,Stashenko P
R960-25 was used in Western Blotting to identify genes upregulated in RANKL-stimulated osteoclast precursor cells.
Tue Jan 01 00:00:00 UTC 2008
The COOH-terminal domain of the JIL-1 histone H3S10 kinase interacts with histone H3 and is required for correct targeting to chromatin.
The Journal of biological chemistry
Bao X,Cai W,Deng H,Zhang W,Krencik R,Girton J,Johansen J,Johansen KM
R960-25 was used in immunohistochemistry to elucidate the function of domains in JIL-1
Fri Nov 21 00:00:00 UTC 2008
A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis.
Cell
Nachury MV,Loktev AV,Zhang Q,Westlake CJ,Peränen J,Merdes A,Slusarski DC,Scheller RH,Bazan JF,Sheffield VC,Jackson PK
R960-25 was used in Western Blotting to show that the vast majority of evolutionarily conserved Bardet-Biedl syndrome (BBS) proteins are present in a stable complex that transiently associates with PCM-1.
Fri Jun 15 00:00:00 UTC 2007
Requirement for O-linked N-acetylglucosaminyltransferase in lymphocytes activation.
The EMBO journal
Golks A,Tran TT,Goetschy JF,Guerini D
R960-25 was used in Western Blotting to demonstrate that O-linked N-acetylglucosaminyltransferase is a central factor for T- and B-lymphocytes activation.
Wed Oct 17 00:00:00 UTC 2007
The p53 Isoform Deltap53 lacks intrinsic transcriptional activity and reveals the critical role of nuclear import in dominant-negative activity.
Cancer research
Chan WM,Poon RY
R960-25 was used in Immunocytochemistry to demonstrate that ectopically expressed Deltap53 neither transactivates the promoters of p21(CIP1/WAF1) or murine double minute-2 nor represses the cyclin B1 promoter in unstressed H1299 cells.
Thu Mar 01 00:00:00 UTC 2007
Activation of p38- and CRM1-dependent nuclear export promotes E2F1 degradation during keratinocyte differentiation.
Oncogene
Ivanova IA,Dagnino L
R960-25 was used in Immunofluorescence to demonstrate that E2F1 downregulation in differentiating keratinocytes involves its ubiquitination, as well as proteasomal degradation subsequent to CRM1-dependent nuclear export.
Thu Feb 22 00:00:00 UTC 2007
A mycobacterium ESX-1-secreted virulence factor with unique requirements for export.
PLoS pathogens
McLaughlin B,Chon JS,MacGurn JA,Carlsson F,Cheng TL,Cox JS,Brown EJ
R960-25 was used in western blot to establish that EspB is a substrate of ESX-1 that is required for Mycobacterium virulence and growth in macrophages
Fri Aug 03 00:00:00 UTC 2007
BRCA1-IRIS activates cyclin D1 expression in breast cancer cells by downregulating the JNK phosphatase DUSP3/VHR.
International journal of cancer
Hao L,ElShamy WM
R960-25 was used in Western Blotting to study an alternate or a complementary pathway by which BRCA1-IRIS activates Cyclin D1 expression.
Sun Jul 01 00:00:00 UTC 2007
The transcription factor nerve growth factor-inducible protein a mediates epigenetic programming: altering epigenetic marks by immediate-early genes.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Weaver IC,D'Alessio AC,Brown SE,Hellstrom IC,Dymov S,Sharma S,Szyf M,Meaney MJ
R960-25 was used in Western Blotting to show that NGFI-A is required for serotonin-induced DNA demethylation and increased exon 1(7) glucocorticoid receptor promoter expression.
Wed Feb 14 00:00:00 UTC 2007
Elevated expression of DecR1 impairs ErbB2/Neu-induced mammary tumor development.
Molecular and cellular biology
Ursini-Siegel J,Rajput AB,Lu H,Sanguin-Gendreau V,Zuo D,Papavasiliou V,Lavoie C,Turpin J,Cianflone K,Huntsman DG,Muller WJ
R960-25 was used in Western Blotting to investigate whether the downregulation of Neu expression is an obligate event in all Neu-transformed breast cancer cells.
Sat Sep 01 00:00:00 UTC 2007
Human pregnancy-associated malaria-specific B cells target polymorphic, conformational epitopes in VAR2CSA.
Molecular microbiology
Barfod L,Bernasconi NL,Dahlbäck M,Jarrossay D,Andersen PH,Salanti A,Ofori MF,Turner L,Resende M,Nielsen MA,Theander TG,Sallusto F,Lanzavecchia A,Hviid L
R960-25 was used in western blot to assess the specificity of naturally acquired immunity to pregnancy-associated malaria
Mon Jan 01 00:00:00 UTC 2007
Wnt3a regulates Lef-1 expression during airway submucosal gland morphogenesis.
Developmental biology
Driskell RR,Goodheart M,Neff T,Liu X,Luo M,Moothart C,Sigmund CD,Hosokawa R,Chai Y,Engelhardt JF
R960-25 was used in Chromatin immunoprecipitation to provide the first in vivo evidence that Wnt3a can transcriptionally regulate the Lef-1 gene in mice.
Tue May 01 00:00:00 UTC 2007
JunB and JunD regulate human heme oxygenase-1 gene expression in renal epithelial cells.
The Journal of biological chemistry
Hock TD,Liby K,Wright MM,McConnell S,Schorpp-Kistner M,Ryan TM,Agarwal A
R960-25 was used in Immunocytochemistry to investigate the regulation of the human haem oxygenase-1 gene in renal epithelial cells.
Fri Mar 02 00:00:00 UTC 2007
Evidence that proteasome-dependent degradation of the retinoblastoma protein in cells lacking A-type lamins occurs independently of gankyrin and MDM2.
PloS one
Nitta RT,Smith CL,Kennedy BK
R960-25 was used in western blot to test if gankyrin and/or MDM2 are required for enhanced pRB degradation in Lmna-/- fibroblasts
Wed Sep 26 00:00:00 UTC 2007
Downstream signaling mechanism of the C-terminal activation domain of transcriptional coactivator CoCoA.
Nucleic acids research
Kim JH,Yang CK,Stallcup MR
R960-25 was used in western blot to characterize the coiled-coil coactivator active domain
Wed Jun 07 00:00:00 UTC 2006
Epitope mapping and topographic analysis of VAR2CSA DBL3X involved in P. falciparum placental sequestration.
PLoS pathogens
Dahlbäck M,Rask TS,Andersen PH,Nielsen MA,Ndam NT,Resende M,Turner L,Deloron P,Hviid L,Lund O,Pedersen AG,Theander TG,Salanti A
R960-25 was used in ELISA to optimize anti-VAR2CSA antibodies
Wed Nov 01 00:00:00 UTC 2006
Epilysin (MMP-28) induces TGF-beta mediated epithelial to mesenchymal transition in lung carcinoma cells.
Journal of cell science
Illman SA,Lehti K,Keski-Oja J,Lohi J
R960-25 was used in Western Blotting to show that epilysin can induce EMT and cell invasion through a TGF-beta-dependent mechanism.
Fri Sep 15 00:00:00 UTC 2006
Laminin alpha 3 forms a complex with beta3 and gamma3 chains that serves as the ligand for alpha 6beta1-integrin at the apical ectoplasmic specialization in adult rat testes.
The Journal of biological chemistry
Yan HHN,Cheng CY
R960-25 was used in Western Blotting to characterise the laminin chains expressed in cells of the seminiferous epithelium in the rat testes.
Fri Jun 23 00:00:00 UTC 2006
MSX2 overexpression inhibits gemcitabine-induced caspase-3 activity in pancreatic cancer cells.
World journal of gastroenterology
Hamada S,Satoh K,Kimura K,Kanno A,Masamune A,Shimosegawa T
R960-25 was used in Western Blotting to show that MSX2 overexpression in Panc-1 cells resulted in decreased gemcitabine-induced caspase-3 activation and increased cell viability under gemcitabine treatment in Px14 cells.
Mon Nov 21 00:00:00 UTC 2005
Truncated ALK derived from chromosomal translocation t(2;5)(p23;q35) binds to the SH3 domain of p85-PI3K.
Mutation research
Polgar D,Leisser C,Maier S,Strasser S,Rüger B,Dettke M,Khorchide M,Simonitsch I,Cerni C,Krupitza G
R960-25 was used in western blot to investigate the molecular interaction between ALK and the regulatory p85 subunit of PI3K
Tue Feb 15 00:00:00 UTC 2005
Proteasomes mediate prolactin-induced receptor down-regulation and fragment generation in breast cancer cells.
The Journal of biological chemistry
Lu JC,Piazza TM,Schuler LA
R960-25 was used in Western Blotting to describe a potential mediator of prolactin action and a novel mechanism whereby proteasomes modulate cellular processes.
Fri Oct 07 00:00:00 UTC 2005
Identification of a family of animal sphingomyelin synthases.
The EMBO journal
Huitema K,van den Dikkenberg J,Brouwers JF,Holthuis JC
R960-25 was used in western blot to identify a novel family of integral membrane proteins involved in sphingomyelin synthesis
Wed Jan 14 00:00:00 UTC 2004
Multimerization and interaction of Toll and Spätzle in Drosophila.
Proceedings of the National Academy of Sciences of the United States of America
Hu X,Yagi Y,Tanji T,Zhou S,Ip YT
R960-25 was used in Western Blotting to identify the mechanism of signalling of toll family receptors in the innate immune response to pathogen-associated molecules.
Tue Jun 22 00:00:00 UTC 2004
Plakoglobin (gamma-catenin) has TCF/LEF family-dependent transcriptional activity in beta-catenin-deficient cell line.
Oncogene
Maeda O,Usami N,Kondo M,Takahashi M,Goto H,Shimokata K,Kusugami K,Sekido Y
R960-25 was used in Western Blotting to study whether plakoglobin has a T-cell factor/lymphocyte enhancer factor family-dependent transcriptional activity.
Thu Jan 29 00:00:00 UTC 2004
TorsinB--perinuclear location and association with torsinA.
Journal of neurochemistry
Hewett JW,Kamm C,Boston H,Beauchamp R,Naismith T,Ozelius L,Hanson PI,Breakefield XO,Ramesh V
R960-25 was used in Western Blotting to conclude that torsinB and torsinA are localized in overlapping cell compartments in the same protein complexes, and may perform related functions.
Tue Jun 01 00:00:00 UTC 2004
Cluster analysis of mass spectrometry data reveals a novel component of SAGA.
Molecular and cellular biology
Powell DW,Weaver CM,Jennings JL,McAfee KJ,He Y,Weil PA,Link AJ
R960-25 was used in western blot to elucidate the regulation of the yeast SAGA complex
Sun Aug 01 00:00:00 UTC 2004
Identification of ubiquitin-interacting proteins in purified polyglutamine aggregates.
FEBS letters
Doi H,Mitsui K,Kurosawa M,Machida Y,Kuroiwa Y,Nukina N
R960-25 was used in Immunocytochemistry to identify ubiquitin-interacting proteins in purified polyglutamine aggregates.
Fri Jul 30 00:00:00 UTC 2004
A PCR-based method for gene deletion and protein tagging in Trypanosoma brucei.
Methods in molecular biology (Clifton, N.J.)
Arhin GK,Shen S,Ullu E,Tschudi C
R960-25 was used in Western Blotting to describe a polymerase chain reaction (PCR)-based method for direct gene deletion and the generation of epitope-tagged fusion proteins.
Wed Jul 28 00:00:00 UTC 2004
Gadd45 proteins induce G2/M arrest and modulate apoptosis in kidney cells exposed to hyperosmotic stress.
The Journal of biological chemistry
Mak SK,Kültz D
R960-25 was used in Immunocytochemistry to show that Gadd45 isoforms function in common but also in distinct pathways during hyperosmolality.
Fri Sep 10 00:00:00 UTC 2004
Role of the coiled-coil coactivator (CoCoA) in aryl hydrocarbon receptor-mediated transcription.
The Journal of biological chemistry
Kim JH,Stallcup MR
R960-25 was used in Chromatin immunoprecipitation to support a physiological role for coiled-coil protein coiled-coil coactivator as a transcriptional coactivator in aryl hydrocarbon receptor/AHR nuclear translocator-mediated transcription.
Fri Nov 26 00:00:00 UTC 2004
Genetic complementation between mutant b subunits in F1F0 ATP synthase.
The Journal of biological chemistry
Grabar TB,Cain BD
R960-25 was used in western blot to develop a system to express and study F(1)F(0) ATP synthase complexes
Fri Jul 23 00:00:00 UTC 2004
Hyaluronidase 2 negatively regulates RON receptor tyrosine kinase and mediates transformation of epithelial cells by jaagsiekte sheep retrovirus.
Proceedings of the National Academy of Sciences of the United States of America
Danilkovitch-Miagkova A,Duh FM,Kuzmin I,Angeloni D,Liu SL,Miller AD,Lerman MI
R960-25 was used in Western blotting following immunoprecipitation to document a novel model of epithelial cell transformation by jaagsiekte sheep retrovirus involving HYAL2-RON receptor tyrosine kinase interaction.
Tue Apr 15 00:00:00 UTC 2003
Dorfin localizes to Lewy bodies and ubiquitylates synphilin-1.
The Journal of biological chemistry
Ito T,Niwa J,Hishikawa N,Ishigaki S,Doyu M,Sobue G
R960-25 was used in immunoprecipitation to study the effect of dorfin-dependent synphilin-1 ubiquitylation in Parkinson's disease and during Lewy body formation
Fri Aug 01 00:00:00 UTC 2003
Ca2+-dependent localization of integrin-linked kinase to cell junctions in differentiating keratinocytes.
The Journal of biological chemistry
Vespa A,Darmon AJ,Turner CE,D'Souza SJ,Dagnino L
R960-25 was used in Western Blotting to show that integrin-linked kinase protein levels are independent of integrin expression and signalling, as they remain constant during Ca(2+)-induced differentiation in murine epidermal keratinocytes.
Fri Mar 28 00:00:00 UTC 2003
Expression of a 28-kilodalton glutathione S-transferase antigen of Schistosoma mansoni on the surface of filamentous phages and evaluation of its vaccine potential.
Clinical and diagnostic laboratory immunology
Rao KV,He YX,Kalyanasundaram R
R960-25 was used in ELISA to describe how phage display can be used as a tool to express candidate vaccine antigens such as the S-transferase antigen of Schistosoma mansoni
Tue Jul 01 00:00:00 UTC 2003
Furin-mediated processing of Pro-C-type natriuretic peptide.
The Journal of biological chemistry
Wu C,Wu F,Pan J,Morser J,Wu Q
R960-25 was used in western blot to investigate the role of furin in human pro-CNP processing
Fri Jul 11 00:00:00 UTC 2003
Fyn and Cdk5 mediate semaphorin-3A signaling, which is involved in regulation of dendrite orientation in cerebral cortex.
Neuron
Sasaki Y,Cheng C,Uchida Y,Nakajima O,Ohshima T,Yagi T,Taniguchi M,Nakayama T,Kishida R,Kudo Y,Ohno S,Nakamura F,Goshima Y
R960-25 was used in western blot to describe a signal transduction pathway in which Fyn and Cdk5 mediate neuronal guidance regulated by Sema3A
Thu Aug 29 00:00:00 UTC 2002
Subcellular localization of the yeast proteome.
Genes & development
Kumar A,Agarwal S,Heyman JA,Matson S,Heidtman M,Piccirillo S,Umansky L,Drawid A,Jansen R,Liu Y,Cheung KH,Miller P,Gerstein M,Roeder GS,Snyder M
R960-25 was used in immunocytochemistry to report the first proteome-scale analysis of protein localization within an eukaryote
Fri Mar 15 00:00:00 UTC 2002
Overexpression, purification, and site-directed spin labeling of the Nramp metal transporter from Mycobacterium leprae.
Proceedings of the National Academy of Sciences of the United States of America
Reeve I,Hummel D,Nelson N,Voss J
R960-25 was used in western blot to characterize the Nramp homologue from Mycobacterium leprae
Tue Jun 25 00:00:00 UTC 2002
Positional cloning of the murine flavivirus resistance gene.
Proceedings of the National Academy of Sciences of the United States of America
Perelygin AA,Scherbik SV,Zhulin IB,Stockman BM,Li Y,Brinton MA
R960-25 was used in western blot to identify Oas1b as a gene that contributes to mouse strain susceptibility to Flaviviruses
Tue Jul 09 00:00:00 UTC 2002
A novel transforming growth factor-beta receptor-interacting protein that is also a light chain of the motor protein dynein.
Molecular biology of the cell
Tang Q,Staub CM,Gao G,Jin Q,Wang Z,Ding W,Aurigemma RE,Mulder KM
R960-25 was used in Western Blotting to identify the mammalian homologue of the LC7 family of dynein light chains as a transforming growth factor-beta receptor-interacting protein.
Sun Dec 01 00:00:00 UTC 2002
UBP43 (USP18) specifically removes ISG15 from conjugated proteins.
The Journal of biological chemistry
Malakhov MP,Malakhova OA,Kim KI,Ritchie KJ,Zhang DE
R960-25 was used in western blot to identify UBP43 is a ISG15-specific protease
Fri Mar 22 00:00:00 UTC 2002
Myostatin inhibits myoblast differentiation by down-regulating MyoD expression.
The Journal of biological chemistry
Langley B,Thomas M,Bishop A,Sharma M,Gilmour S,Kambadur R
R960-25 was used in Immunoprecipitation to propose that myostatin plays a critical role in myogenic differentiation.
Fri Dec 20 00:00:00 UTC 2002
Inhibition of adipocyte differentiation by resistin-like molecule alpha. Biochemical characterization of its oligomeric nature.
The Journal of biological chemistry
Blagoev B,Kratchmarova I,Nielsen MM,Fernandez MM,Voldby J,Andersen JS,Kristiansen K,Pandey A,Mann M
R960-25 was used in western blot to investigate the interactions of RELMalpha in adipogenesis and muscle differentiation
Fri Nov 01 00:00:00 UTC 2002
Silencing of transcription of the human luteinizing hormone receptor gene by histone deacetylase-mSin3A complex.
The Journal of biological chemistry
Zhang Y,Dufau ML
R960-25 was used in western blot to study the role of the HDAC-mSin3A complex during hLHR gene transcription
Fri Sep 06 00:00:00 UTC 2002
Overexpressed human mitochondrial thioredoxin confers resistance to oxidant-induced apoptosis in human osteosarcoma cells.
The Journal of biological chemistry
Chen Y,Cai J,Murphy TJ,Jones DP
R960-25 was used in western blot to study the localization and function of human mtTrx using 143B human osteosarcoma cells
Fri Sep 06 00:00:00 UTC 2002
Integrin-linked kinase regulates inducible nitric oxide synthase and cyclooxygenase-2 expression in an NF-kappa B-dependent manner.
The Journal of biological chemistry
Tan C,Mui A,Dedhar S
R960-25 was used in western blot to investigate the role of integrin-linked kinase in LPS-stimulated macrophages
Fri Feb 01 00:00:00 UTC 2002
EMR4, a novel epidermal growth factor (EGF)-TM7 molecule up-regulated in activated mouse macrophages, binds to a putative cellular ligand on B lymphoma cell line A20.
The Journal of biological chemistry
Stacey M,Chang GW,Sanos SL,Chittenden LR,Stubbs L,Gordon S,Lin HH
R960-25 was used in western blot to identify and characterize a novel member of the EGF-TM7 family, mEMR4
Fri Aug 09 00:00:00 UTC 2002
Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex.
The Journal of biological chemistry
Vainauskas S,Maeda Y,Kurniawan H,Kinoshita T,Menon AK
R960-25 was used in immunoprecipitation to perform structure-function analyses of Gaa1
Fri Aug 23 00:00:00 UTC 2002
RYK, a catalytically inactive receptor tyrosine kinase, associates with EphB2 and EphB3 but does not interact with AF-6.
The Journal of biological chemistry
Trivier E,Ganesan TS
R960-25 was used in immunoprecipitation and western blot to demonstrate that human RYK associates with EphB2 and EphB3 but is not phosphorylated by them
Fri Jun 21 00:00:00 UTC 2002
Characterization of a novel salivary immunosuppressive protein from Ixodes ricinus ticks.
The Journal of biological chemistry
Leboulle G,Crippa M,Decrem Y,Mejri N,Brossard M,Bollen A,Godfroid E
R960-25 was used in western blot to determine the immunomodulator activity of Ixodes ricinus immunosuppressor (Iris)
Fri Mar 22 00:00:00 UTC 2002
A Forkhead/winged helix-related transcription factor mediates insulin-increased plasminogen activator inhibitor-1 gene transcription.
The Journal of biological chemistry
Vulin AI,Stanley FM
R960-25 was used in western blot to elucidate the mechanism of insulin-activated PAI-1 transcription
Fri Jun 07 00:00:00 UTC 2002
RasGRP4, a new mast cell-restricted Ras guanine nucleotide-releasing protein with calcium- and diacylglycerol-binding motifs. Identification of defective variants of this signaling protein in asthma, mastocytosis, and mast cell leukemia patients and demonstration of the importance of RasGRP4 in mast cell development and function.
The Journal of biological chemistry
Yang Y,Li L,Wong GW,Krilis SA,Madhusudhan MS,Sali A,Stevens RL
R960-25 was used in western blot to describe the role of RasGRP4 in the final stages of MC development
Fri Jul 12 00:00:00 UTC 2002
Cyclin G2 associates with protein phosphatase 2A catalytic and regulatory B' subunits in active complexes and induces nuclear aberrations and a G1/S phase cell cycle arrest.
The Journal of biological chemistry
Bennin DA,Don AS,Brake T,McKenzie JL,Rosenbaum H,Ortiz L,DePaoli-Roach AA,Horne MC
R960-25 was used in immunocytochemistry to determine the role of G2-PP2A complexes during cell cycle progression
Fri Jul 26 00:00:00 UTC 2002
Processing of pro-atrial natriuretic peptide by corin in cardiac myocytes.
The Journal of biological chemistry
Wu F,Yan W,Pan J,Morser J,Wu Q
R960-25 was used in immunoprecipitation and western blot to show that corin is the endogenous pro-ANP convertase in cardiomyocytes
Fri May 10 00:00:00 UTC 2002
Identification and characterization of Moca-cyp. A Drosophila melanogaster nuclear cyclophilin.
The Journal of biological chemistry
Cavarec L,Kamphausen T,Dubourg B,Callebaut I,Lemeunier F,Métivier D,Feunteun J,Fischer G,Modjtahedi N
R960-25 was used in immunocytochemistry and immunoprecipitation to characterize the novel cyclophilin, Moca-cyp, isolated from Drosophila melanogaster
Fri Oct 25 00:00:00 UTC 2002
Chimaerins, novel non-protein kinase C phorbol ester receptors, associate with Tmp21-I (p23): evidence for a novel anchoring mechanism involving the chimaerin C1 domain.
The Journal of biological chemistry
Wang H,Kazanietz MG
R960-25 was used in immunocytochemistry and western blot to investigate the relationship between Tmp21-I (p23) and chimaerins
Fri Feb 08 00:00:00 UTC 2002
Identification of novel SH3 domain ligands for the Src family kinase Hck. Wiskott-Aldrich syndrome protein (WASP), WASP-interacting protein (WIP), and ELMO1.
The Journal of biological chemistry
Scott MP,Zappacosta F,Kim EY,Annan RS,Miller WT
R960-25 was used in western blot to identify binding partners for the SH3 domain of Hck using the monocyte cell line U937
Fri Aug 02 00:00:00 UTC 2002
Mechanisms of regulation of phospholipase D1 and D2 by the heterotrimeric G proteins G13 and Gq.
The Journal of biological chemistry
Xie Z,Ho WT,Spellman R,Cai S,Exton JH
R960-25 was used in western blot to study the effect of phosphorylation and palmitoylation on the activation of rPLD1 by constitutively active Galpha(13)Q226L and Galpha(q)Q209L
Fri Apr 05 00:00:00 UTC 2002
Identification of a matrix-binding domain in MAGP1 and MAGP2 and intracellular localization of alternative splice forms.
The Journal of biological chemistry
Segade F,Trask BC,Broekelmann TJ,Pierce RA,Mecham RP
R960-25 was used in immunocytochemistry to elucidate which residues of MAGP1 are needed for interactions with other microfibrillar proteins
Fri Mar 29 00:00:00 UTC 2002
Rrn3 phosphorylation is a regulatory checkpoint for ribosome biogenesis.
The Journal of biological chemistry
Cavanaugh AH,Hirschler-Laszkiewicz I,Hu Q,Dundr M,Smink T,Misteli T,Rothblum LI
R960-25 was used in western blot to determine that the phosphorylation state of Rrn3 regulates rDNA transcription
Fri Jul 26 00:00:00 UTC 2002
Biosynthesis and cellular trafficking of the convertase SKI-1/S1P: ectodomain shedding requires SKI-1 activity.
The Journal of biological chemistry
Elagoz A,Benjannet S,Mammarbassi A,Wickham L,Seidah NG
R960-25 was used in western blot to elucidate the domains that control SKI-1 activity and cellular trafficking
Fri Mar 29 00:00:00 UTC 2002
Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins.
The Journal of biological chemistry
Gubitz AK,Mourelatos Z,Abel L,Rappsilber J,Mann M,Dreyfuss G
R960-25 was used in immunoprecipitation and western blot to identify Gemin5 as a component of the survival of motor neurons (SMN) complex
Fri Feb 15 00:00:00 UTC 2002
Functional interaction of caveolin-1 with Bruton's tyrosine kinase and Bmx.
The Journal of biological chemistry
Vargas L,Nore BF,Berglof A,Heinonen JE,Mattsson PT,Smith CI,Mohamed AJ
R960-25 was used in immunoprecipitation to investigate caveolin-1 and Btk interactions in B cells
Fri Mar 15 00:00:00 UTC 2002
Membrane targeting of ATP-sensitive potassium channel. Effects of glycosylation on surface expression.
The Journal of biological chemistry
Conti LR,Radeke CM,Vandenberg CA
R960-25 was used in western blot to determine the relationship between the N-linked glycosylation sites (Asn(10) and Asn(1050)) and SUR1 trafficking
Fri Jul 12 00:00:00 UTC 2002
Suppression of Ras-mediated tumorigenicity and metastasis through inhibition of the Met receptor tyrosine kinase.
Proceedings of the National Academy of Sciences of the United States of America
Furge KA,Kiewlich D,Le P,Vo MN,Faure M,Howlett AR,Lipson KE,Vande Woude GF,Webb CP
R960-25 was used in western blot to investigate the role of Met during Ras-mediated tumor growth and metastasis
Tue Sep 11 00:00:00 UTC 2001
Nuclear envelope proteomics: novel integral membrane proteins of the inner nuclear membrane.
Proceedings of the National Academy of Sciences of the United States of America
Dreger M,Bengtsson L,Schöneberg T,Otto H,Hucho F
R960-25 was used in immunocytochemistry to identify novel nuclear envelope proteins using gel electrophoresis and mass spectrometry
Tue Oct 09 00:00:00 UTC 2001
Drosophila MyD88 is an adapter in the Toll signaling pathway.
Proceedings of the National Academy of Sciences of the United States of America
Horng T,Medzhitov R
R960-25 was used in western blot to characterize a Drosophila homologue of human MyD88 and determine its role in caspase activation
Tue Oct 23 00:00:00 UTC 2001
A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via its RING domain.
The Journal of biological chemistry
Dai KS,Liew CC
R960-25 was used in immunocytochemistry to investigate the localization and interactions of striated muscle RING zinc finger protein (SMRZ)
Fri Jun 29 00:00:00 UTC 2001
TANK2, a new TRF1-associated poly(ADP-ribose) polymerase, causes rapid induction of cell death upon overexpression.
The Journal of biological chemistry
Kaminker PG,Kim SH,Taylor RD,Zebarjadian Y,Funk WD,Morin GB,Yaswen P,Campisi J
R960-25 was used in Western Blotting to characterise a second human tankyrase-like protein, tankyrase 2 (TANK2), in vitro.
Fri Sep 21 00:00:00 UTC 2001
MdmX binding to ARF affects Mdm2 protein stability and p53 transactivation.
The Journal of biological chemistry
Jackson MW,Lindstrom MS,Berberich SJ
R960-25 was used in immunoprecipitation to demonstrate that the interaction between MdmX and ARF regulate Mdm2 protein levels
Fri Jul 06 00:00:00 UTC 2001
hCds1-mediated phosphorylation of BRCA1 regulates the DNA damage response.
Nature
Lee JS,Collins KM,Brown AL,Lee CH,Chung JH
R960-25 was used in immunoprecipitation to investigate the relationship between Cds1 kinase (hCds1/Chk2) and BRCA1 during DNA damage
Thu Mar 09 00:00:00 UTC 2000
Corin, a transmembrane cardiac serine protease, acts as a pro-atrial natriuretic peptide-converting enzyme.
Proceedings of the National Academy of Sciences of the United States of America
Yan W,Wu F,Morser J,Wu Q
R960-25 was used in immunoprecipitation and western blot to identify corin as a pro-ANP-converting enzyme
Tue Jul 18 00:00:00 UTC 2000
A protein kinase encoded by the t complex responder gene causes non-mendelian inheritance.
Nature
Herrmann BG,Koschorz B,Wertz K,McLaughlin KJ,Kispert A
R960-25 was used in western blot to identify Tcr and study its role in non-mendelian transmission of chromosomes
Thu Nov 11 00:00:00 UTC 1999
Substrate targeting of the yeast cyclin-dependent kinase Pho85p by the cyclin Pcl10p.
Molecular and cellular biology
Wilson WA,Mahrenholz AM,Roach PJ
R960-25 was used in ChIP assay to study the interactions between Gsy2p and Pho85p and Pcl10p
Fri Oct 01 00:00:00 UTC 1999
