Proteintech

VAMP2 Polyclonal Antibody

VAMP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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VAMP2 Antibody (10135-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using (Product # 10135-1-AP) VAMP2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Product Image Gallery

Product Details

10135-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

VAMP2 Fusion Protein Ag0179 (1-116 aa encoded by BC002737)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSATAATAP PAAPAGEGGP PAPPPNLTSN RRLQQTQAQV DEVVDIMRVN VDKVLERDQK LSELDDRADA LQAGASQFET SAAKLKRKYW WKNLKMMIIL GVICAIILII IIVYFST (1-116 aa encoded by BC002737)

Target Information

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP-2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GEARBOCS: An Adeno Associated Virus Tool for In Vivo Gene Editing in Astrocytes.

bioRxiv : the preprint server for biology

Bindu DS,Savage JT,Brose N,Bradley L,Dimond K,Tan CX,Eroglu C

Thu Oct 10 00:00:00 UTC 2024

Cofilin promotes tau pathology in Alzheimer's disease.

Cell reports

Yan M,Tang L,Dai L,Lei C,Xiong M,Zhang X,He M,Tian Y,Xiong J,Ke W,Zhang Z,Zhang C,Deng X,Zhang Z

Tue Feb 28 00:00:00 UTC 2023

Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Raj N,Greune L,Kahms M,Mildner K,Franzkoch R,Psathaki OE,Zobel T,Zeuschner D,Klingauf J,Gerke V

Mon May 01 00:00:00 UTC 2023

Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Raj N,Greune L,Kahms M,Mildner K,Franzkoch R,Psathaki OE,Zobel T,Zeuschner D,Klingauf J,Gerke V

Mon May 01 00:00:00 UTC 2023

Exogenous H₂S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions.

International journal of molecular sciences

Liu N,Li M,Liu S,Kang J,Chen L,Huang J,Wang Y,Chen H,Zhang W

Sat Jan 14 00:00:00 UTC 2023

Exogenous H₂S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions.

International journal of molecular sciences

Liu N,Li M,Liu S,Kang J,Chen L,Huang J,Wang Y,Chen H,Zhang W

Sat Jan 14 00:00:00 UTC 2023

Exogenous H₂S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions.

International journal of molecular sciences

Liu N,Li M,Liu S,Kang J,Chen L,Huang J,Wang Y,Chen H,Zhang W

Sat Jan 14 00:00:00 UTC 2023

A hepatokine derived from the ER protein CREBH promotes triglyceride metabolism by stimulating lipoprotein lipase activity.

Science signaling

Kim H,Song Z,Zhang R,Davies BSJ,Zhang K

Tue Jan 17 00:00:00 UTC 2023

Tau accelerates α-synuclein aggregation and spreading in Parkinson's disease.

Brain : a journal of neurology

Pan L,Li C,Meng L,Tian Y,He M,Yuan X,Zhang G,Zhang Z,Xiong J,Chen G,Zhang Z

Fri Oct 21 00:00:00 UTC 2022

Tau accelerates α-synuclein aggregation and spreading in Parkinson's disease.

Brain : a journal of neurology

Pan L,Li C,Meng L,Tian Y,He M,Yuan X,Zhang G,Zhang Z,Xiong J,Chen G,Zhang Z

Fri Oct 21 00:00:00 UTC 2022

Autism risk gene KMT5B deficiency in prefrontal cortex induces synaptic dysfunction and social deficits via alterations of DNA repair and gene transcription.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology

Wang ZJ,Rein B,Zhong P,Williams J,Cao Q,Yang F,Zhang F,Ma K,Yan Z

Sun Aug 01 00:00:00 UTC 2021

Transcriptomic analysis of human brains with Alzheimer's disease reveals the altered expression of synaptic genes linked to cognitive deficits.

Brain communications

Williams JB,Cao Q,Yan Z

Mon May 06 00:00:00 UTC 2024

Transcriptomic analysis of human brains with Alzheimer's disease reveals the altered expression of synaptic genes linked to cognitive deficits.

Brain communications

Williams JB,Cao Q,Yan Z

Mon May 06 00:00:00 UTC 2024

Proteomics Study Reveals the Anti-Depressive Mechanisms and the Compatibility Advantage of Chaihu-Shugan-San in a Rat Model of Chronic Unpredictable Mild Stress.

Frontiers in pharmacology

Zhu X,Li T,Hu E,Duan L,Zhang C,Wang Y,Tang T,Yang Z,Fan R

Fri Feb 04 00:00:00 UTC 2022

Neuromodulation Induced by Sitagliptin: A New Strategy for Treating Diabetic Retinopathy.

Biomedicines

Ramos H,Bogdanov P,Sabater D,Huerta J,Valeri M,Hernández C,Simó R

Fri Nov 26 00:00:00 UTC 2021

Neuromodulation Induced by Sitagliptin: A New Strategy for Treating Diabetic Retinopathy.

Biomedicines

Ramos H,Bogdanov P,Sabater D,Huerta J,Valeri M,Hernández C,Simó R

Fri Nov 26 00:00:00 UTC 2021

Hepatic Synaptotagmin 1 is involved in the remodelling of liver plasma- membrane lipid composition and gene expression in male Apoe-deficient mice consuming a Western diet.

Biochimica et biophysica acta. Molecular and cell biology of lipids

Sancho-Knapik S,Pastor O,Barranquero C,Herrera Marcos LV,Guillén N,Arnal C,Gascón S,Navarro MA,Rodríguez-Yoldi MJ,Busto R,Lasunción MA,Osada J

Tue Dec 01 00:00:00 UTC 2020

Loss of the voltage-gated proton channel Hv1 decreases insulin secretion and leads to hyperglycemia and glucose intolerance in mice.

The Journal of biological chemistry

Pang H,Wang X,Zhao S,Xi W,Lv J,Qin J,Zhao Q,Che Y,Chen L,Li SJ

Fri Mar 13 00:00:00 UTC 2020

Chronic hepatitis C virus infection impairs insulin secretion by regulation of p38δ MAPK-dependent exocytosis in pancreatic β-cells.

Clinical science (London, England : 1979)

Chen J,Wang F,Zhou Y,Jiang J,Ksimu S,Zhang X,Li JZ,Niu J,Wang Q

Fri Mar 13 00:00:00 UTC 2020

SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Neuroscience

Duan XL,Guo Z,He YT,Li YX,Liu YN,Bai HH,Li HL,Hu XD,Suo ZW

Sun Mar 01 00:00:00 UTC 2020

Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.

The Journal of biological chemistry

Merriman C,Fu D

Fri Nov 08 00:00:00 UTC 2019

Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome.

Neuron

Cagnetta R,Frese CK,Shigeoka T,Krijgsveld J,Holt CE

Wed Jul 11 00:00:00 UTC 2018

Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.

The Journal of biological chemistry

Aerbajinai W,Liu L,Zhu J,Kumkhaek C,Chin K,Rodgers GP

Fri Apr 15 00:00:00 UTC 2016

Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.

The Journal of biological chemistry

Aerbajinai W,Liu L,Zhu J,Kumkhaek C,Chin K,Rodgers GP

Fri Apr 15 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Synaptobrevin; synaptobrevin 2; Synaptobrevin 2 (vesicle-associated membrane protein VAMP-2); synaptobrevin II; Synaptobrevin-2; vamp; VAMP-2; vesicle-associated membrane 2; Vesicle-associated membrane protein (synaptobrevin 2); Vesicle-associated membrane protein 2; vesicle-associated membrane protein 2 (synaptobrevin 2)

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Gene Aliases: RATVAMPB; RATVAMPIR; SYB; Syb-2; SYB2; sybII; VAMP-2; VAMP2

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UniProt ID: (Human) P63027, (Mouse) P63044, (Rat) P63045

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Entrez Gene ID: (Human) 6844, (Mouse) 22318, (Rat) 24803

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Function(s)

SNARE binding SNAP receptor activity protein binding calmodulin binding phospholipid binding protein C-terminus binding myosin binding syntaxin-1 binding syntaxin binding protein complex binding identical protein binding protein self-association ion channel binding calcium-dependent protein binding

Process(es)

protein complex assembly vesicle fusion response to glucose protein transport synaptic vesicle exocytosis vesicle-mediated transport calcium ion regulated exocytosis regulation of exocytosis regulation of calcium ion-dependent exocytosis cellular response to insulin stimulus Golgi to plasma membrane protein transport eosinophil degranulation long-term synaptic potentiation regulation of vesicle-mediated transport membrane fusion positive regulation of intracellular protein transport regulation of delayed rectifier potassium channel activity neurotransmitter secretion neutrophil degranulation natural killer cell degranulation mucus secretion regulation of histamine secretion by mast cell exocytosis post-Golgi vesicle-mediated transport glutamate secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VAMP2 Polyclonal Antibody

VAMP2 Antibody (10135-1-AP) in IHC (P)

Product Details

10135-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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