Invitrogen

VAMP4 Polyclonal Antibody

VAMP4 Antibody in Immunocytochemistry (ICC/IF)

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VAMP4 Antibody (PA1-768) in ICC/IF

Immunofluorescence analysis of VAMP4 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with VAMP4 Rabbit Polyclonal Antibody (Product # PA1-768) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More

VAMP4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VAMP4 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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VAMP4 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of VAMP4 was observed in Mouse Brain, Rat Brain, Rat Lung and Rat Liver in Western Blot using VAMP4 Polyclonal Antibody (Product # PA1-768). VAMP4 is reported to be expressed in brain tissue and not in other tissues like lung and liver. {RE}

Product Details

PA1-768

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

2 µg/mL

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant rat VAMP-4 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2212790

Product Specific Information

PA1-768 detects VAMP-4 from human and mouse samples.

PA1-768 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~18 kDa protein representing VAMP-4 from PC3 cell extract. Immunofluorescent staining of VAMP-4 in CV-1 cells using PA1-768 results in a staining pattern consistent with VAMP-4 staining.

Immunofluorescence staining with this antibody requires saponin permeabilization.

The PA1-768 antigen is recombinant rat VAMP-4.

Target Information

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, VAMP levels of all isoforms appear to be significantly lowered.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SNAP23 deficiency causes severe brain dysplasia through the loss of radial glial cell polarity.

The Journal of cell biology

Kunii M,Noguchi Y,Yoshimura SI,Kanda S,Iwano T,Avriyanti E,Atik N,Sato T,Sato K,Ogawa M,Harada A

Published figure using VAMP4 polyclonal antibody (Product # PA1-768) in Immunocytochemistry

Mon Jan 04 00:00:00 UTC 2021

SNAP23 deficiency causes severe brain dysplasia through the loss of radial glial cell polarity.

The Journal of cell biology

Kunii M,Noguchi Y,Yoshimura SI,Kanda S,Iwano T,Avriyanti E,Atik N,Sato T,Sato K,Ogawa M,Harada A

Published figure using VAMP4 polyclonal antibody (Product # PA1-768) in Immunocytochemistry

Mon Jan 04 00:00:00 UTC 2021

Control of insulin granule formation and function by the ABC transporters ABCG1 and ABCA1 and by oxysterol binding protein OSBP.

Molecular biology of the cell

Hussain SS,Harris MT,Kreutzberger AJB,Inouye CM,Doyle CA,Castle AM,Arvan P,Castle JD

PA1-768 was used in Immunohistochemistry-immunofluorescence to study the role of cholesterol in supporting granule membrane formation and function.

Tue May 15 00:00:00 UTC 2018

Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins.

Histochemistry and cell biology

Tajika Y,Takahashi M,Khairani AF,Ueno H,Murakami T,Yorifuji H

PA1-768 was used in immunocytochemistry and western blot to study the morphology of the myotube vesicular transport system

Tue Apr 01 00:00:00 UTC 2014

An intracellular role for ABCG1-mediated cholesterol transport in the regulated secretory pathway of mouse pancreatic beta cells.

The Journal of clinical investigation

Sturek JM,Castle JD,Trace AP,Page LC,Castle AM,Evans-Molina C,Parks JS,Mirmira RG,Hedrick CC

PA1-768 was used in western blot to investigate the role of ABCG1 in subcellular cholesterol distribution in beta cells

Thu Jul 01 00:00:00 UTC 2010

VAMP2 marks quiescent satellite cells and myotubes, but not activated myoblasts.

Acta histochemica et cytochemica

Tajika Y,Takahashi M,Hino M,Murakami T,Yorifuji H

PA1-768 was used in immunocytochemistry to investigate VAMP2 expression and its cellular distribution during muscle differentiation

Fri Aug 27 00:00:00 UTC 2010

Interaction of calcium-dependent activator protein for secretion 1 (CAPS1) with the class II ADP-ribosylation factor small GTPases is required for dense-core vesicle trafficking in the trans-Golgi network.

The Journal of biological chemistry

Sadakata T,Shinoda Y,Sekine Y,Saruta C,Itakura M,Takahashi M,Furuichi T

Published figure using VAMP4 polyclonal antibody (Product # PA1-768) in Immunocytochemistry

Fri Dec 03 00:00:00 UTC 2010

The Journal of biological chemistry

Sadakata T,Shinoda Y,Sekine Y,Saruta C,Itakura M,Takahashi M,Furuichi T

Published figure using VAMP4 polyclonal antibody (Product # PA1-768) in Immunocytochemistry

Fri Dec 03 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: vamp; VAMP-4; vesicle associated membrane protein 4; Vesicle-associated membrane protein 4

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Gene Aliases: D1Ertd147e; VAMP-4; VAMP24; VAMP4

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UniProt ID: (Human) O75379, (Mouse) O70480

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Entrez Gene ID: (Human) 8674, (Mouse) 53330, (Rat) 364033

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Function(s)

SNARE binding SNAP receptor activity protein binding

Process(es)

microtubule cytoskeleton organization exocytosis vesicle fusion vesicle-mediated transport SNARE complex assembly regulation of Golgi to plasma membrane protein transport Golgi to plasma membrane protein transport Golgi ribbon formation toxin transport ER to Golgi vesicle-mediated transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VAMP4 Polyclonal Antibody

VAMP4 Antibody (PA1-768) in ICC/IF

Product Details

PA1-768

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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