Proteintech

VAPB Polyclonal Antibody

VAPB Antibody in Immunocytochemistry (ICC/IF)

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VAPB Antibody (14477-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VAPB antibody (Product # 14477-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

VAPB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VAPB Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14477-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 25 publications 25 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 7 publications 7 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

VAPB Fusion Protein Ag5857 (31-180 aa encoded by BC001712)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KLGNPTDRN VCFKVKTTAP RRYCVRPNSG IIDAGASINV SVMLQPFDYD PNEKSKHKFM VQSMFAPTDT SDMEAVWKEA KPEDLMDSKL RCVFELPAEN DKPHDVEINK IISTTASKTE TPIVSKSLSS SLDDTEVKKV MEECKRLQGE V (31-180 aa encoded by BC001712)

Target Information

Vesicle-associated membrane protein-associated protein B/C is a protein that in humans is encoded by the VAPB gene. The VAPB gene is found on the 20th human chromosome. The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Elucidation of OSW-1-Induced Stress Responses in Neuro2a Cells.

International journal of molecular sciences

Oh-Hashi K,Nakamura H,Ogawa H,Hirata Y,Sakurai K

Fri Mar 17 00:00:00 UTC 2023

Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats.

Cell death & disease

Wang Y,Miao Z,Xu C,Cai Y,Yang Y,Hu Y,Zhao M,Shao Y,Li Z,Chen J,Chen S,Wang L

Sat May 13 00:00:00 UTC 2023

The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.

Nature communications

Ganji R,Paulo JA,Xi Y,Kline I,Zhu J,Clemen CS,Weihl CC,Purdy JG,Gygi SP,Raman M

Mon Feb 06 00:00:00 UTC 2023

Deficiency of Perry syndrome-associated p150^Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.

NPJ Parkinson's disease

Yu J,Yang X,Zheng J,Sgobio C,Sun L,Cai H

Tue Mar 07 00:00:00 UTC 2023

Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome.

Developmental cell

Geng J,Khaket TP,Pan J,Li W,Zhang Y,Ping Y,Cobos Sillero MI,Lu B

Mon Apr 10 00:00:00 UTC 2023

A SNX1-SNX2-VAPB partnership regulates endosomal membrane rewiring in response to nutritional stress.

Life science alliance

Da Graça J,Charles J,Djebar M,Alvarez-Valadez K,Botti J,Morel E

Wed Mar 01 00:00:00 UTC 2023

Oligomeric CHMP7 mediates three-way ER junctions and ER-mitochondria interactions.

Cell death and differentiation

Chu Q,Wang J,Du Y,Zhou T,Shi A,Xiong J,Ji WK,Deng L

Sun Jan 01 00:00:00 UTC 2023

Serine palmitoyltransferase assembles at ER-mitochondria contact sites.

Life science alliance

Aaltonen MJ,Alecu I,König T,Bennett SA,Shoubridge EA

Tue Feb 01 00:00:00 UTC 2022

Proteomic Mapping and Targeting of Mitotic Pericentriolar Material in Tumors Bearing Centrosome Amplification.

Cancer research

Xie B,Pu Y,Yang F,Chen W,Yue W,Ma J,Zhang N,Jiang Y,Wu J,Lin Y,Liang X,Wang C,Zou P,Li M

Mon Jul 18 00:00:00 UTC 2022

SPTLC1 variants associated with ALS produce distinct sphingolipid signatures through impaired interaction with ORMDL proteins.

The Journal of clinical investigation

Lone MA,Aaltonen MJ,Zidell A,Pedro HF,Morales Saute JA,Mathew S,Mohassel P,Bönnemann CG,Shoubridge EA,Hornemann T

Thu Sep 15 00:00:00 UTC 2022

The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo.

Journal of cell science

Han S,Zhao F,Hsia J,Ma X,Liu Y,Torres S,Fujioka H,Zhu X

Thu Jul 01 00:00:00 UTC 2021

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Nature cell biology

König T,Nolte H,Aaltonen MJ,Tatsuta T,Krols M,Stroh T,Langer T,McBride HM

Wed Dec 01 00:00:00 UTC 2021

The Novel ALG-2 Target Protein CDIP1 Promotes Cell Death by Interacting with ESCRT-I and VAPA/B.

International journal of molecular sciences

Inukai R,Mori K,Kuwata K,Suzuki C,Maki M,Takahara T,Shibata H

Mon Jan 25 00:00:00 UTC 2021

The Novel ALG-2 Target Protein CDIP1 Promotes Cell Death by Interacting with ESCRT-I and VAPA/B.

International journal of molecular sciences

Inukai R,Mori K,Kuwata K,Suzuki C,Maki M,Takahara T,Shibata H

Mon Jan 25 00:00:00 UTC 2021

VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions.

Molecular biology of the cell

Du Y,Wang J,Xiong J,Fang N,Ji WK

Sun Aug 01 00:00:00 UTC 2021

VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions.

Molecular biology of the cell

Du Y,Wang J,Xiong J,Fang N,Ji WK

Sun Aug 01 00:00:00 UTC 2021

Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.

EMBO reports

Spits M,Heesterbeek IT,Voortman LM,Akkermans JJ,Wijdeven RH,Cabukusta B,Neefjes J

Wed Mar 03 00:00:00 UTC 2021

A High-Density Human Mitochondrial Proximity Interaction Network.

Cell metabolism

Antonicka H,Lin ZY,Janer A,Aaltonen MJ,Weraarpachai W,Gingras AC,Shoubridge EA

Tue Sep 01 00:00:00 UTC 2020

Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.

Scientific reports

Yamanaka T,Nishiyama R,Shimogori T,Nukina N

Wed May 06 00:00:00 UTC 2020

Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.

Scientific reports

Yamanaka T,Nishiyama R,Shimogori T,Nukina N

Wed May 06 00:00:00 UTC 2020

MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.

Molecular cell

Freyre CAC,Rauher PC,Ejsing CS,Klemm RW

Thu Dec 05 00:00:00 UTC 2019

Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.

Journal of virology

Wang H,Tai AW

Fri Nov 15 00:00:00 UTC 2019

A trap mutant reveals the physiological client spectrum of TRC40.

Journal of cell science

Coy-Vergara J,Rivera-Monroy J,Urlaub H,Lenz C,Schwappach B

Mon Jul 01 00:00:00 UTC 2019

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Cell research

Zheng P,Chen Q,Tian X,Qian N,Chai P,Liu B,Hu J,Blackstone C,Zhu D,Teng J,Chen J

Wed Aug 01 00:00:00 UTC 2018

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Cell research

Zheng P,Chen Q,Tian X,Qian N,Chai P,Liu B,Hu J,Blackstone C,Zhu D,Teng J,Chen J

Wed Aug 01 00:00:00 UTC 2018

Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner.

Neuroscience bulletin

Wu D,Hao Z,Ren H,Wang G

Sat Dec 01 00:00:00 UTC 2018

Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner.

Neuroscience bulletin

Wu D,Hao Z,Ren H,Wang G

Sat Dec 01 00:00:00 UTC 2018

Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner.

Neuroscience bulletin

Wu D,Hao Z,Ren H,Wang G

Sat Dec 01 00:00:00 UTC 2018

Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner.

Neuroscience bulletin

Wu D,Hao Z,Ren H,Wang G

Sat Dec 01 00:00:00 UTC 2018

Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.

The Journal of biological chemistry

Young PA,Senkal CE,Suchanek AL,Grevengoed TJ,Lin DD,Zhao L,Crunk AE,Klett EL,Füllekrug J,Obeid LM,Coleman RA

Fri Oct 26 00:00:00 UTC 2018

Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.

The Journal of biological chemistry

Young PA,Senkal CE,Suchanek AL,Grevengoed TJ,Lin DD,Zhao L,Crunk AE,Klett EL,Füllekrug J,Obeid LM,Coleman RA

Fri Oct 26 00:00:00 UTC 2018

Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.

The Journal of cell biology

Simonetti B,Danson CM,Heesom KJ,Cullen PJ

Mon Nov 06 00:00:00 UTC 2017

Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.

The Journal of cell biology

Simonetti B,Danson CM,Heesom KJ,Cullen PJ

Mon Nov 06 00:00:00 UTC 2017

Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum.

Journal of virology

Cianciola NL,Chung S,Manor D,Carlin CR

Wed Mar 15 00:00:00 UTC 2017

Neuronal overexpression of human VAPB slows motor impairment and neuromuscular denervation in a mouse model of ALS.

Human molecular genetics

Kim JY,Jang A,Reddy R,Yoon WH,Jankowsky JL

Tue Nov 01 00:00:00 UTC 2016

Neuronal overexpression of human VAPB slows motor impairment and neuromuscular denervation in a mouse model of ALS.

Human molecular genetics

Kim JY,Jang A,Reddy R,Yoon WH,Jankowsky JL

Tue Nov 01 00:00:00 UTC 2016

The ATF6 pathway of the ER stress response contributes to enhanced viability in glioblastoma.

Oncotarget

Dadey DY,Kapoor V,Khudanyan A,Urano F,Kim AH,Thotala D,Hallahan DE

Tue Jan 12 00:00:00 UTC 2016

The ATF6 pathway of the ER stress response contributes to enhanced viability in glioblastoma.

Oncotarget

Dadey DY,Kapoor V,Khudanyan A,Urano F,Kim AH,Thotala D,Hallahan DE

Tue Jan 12 00:00:00 UTC 2016

The role of endoplasmic reticulum-related BiP/GRP78 in interferon gamma-induced persistent Chlamydia pneumoniae infection.

Cellular microbiology

Shima K,Klinger M,Schütze S,Kaufhold I,Solbach W,Reiling N,Rupp J

Wed Jul 01 00:00:00 UTC 2015

The role of endoplasmic reticulum-related BiP/GRP78 in interferon gamma-induced persistent Chlamydia pneumoniae infection.

Cellular microbiology

Shima K,Klinger M,Schütze S,Kaufhold I,Solbach W,Reiling N,Rupp J

Wed Jul 01 00:00:00 UTC 2015

Expression of vesicle-associated membrane-protein-associated protein B cleavage products in peripheral blood leukocytes and cerebrospinal fluid of patients with sporadic amyotrophic lateral sclerosis.

European journal of neurology

Deidda I,Galizzi G,Passantino R,Cascio C,Russo D,Colletti T,La Bella V,Guarneri P

Sat Mar 01 00:00:00 UTC 2014

European journal of neurology

Deidda I,Galizzi G,Passantino R,Cascio C,Russo D,Colletti T,La Bella V,Guarneri P

Sat Mar 01 00:00:00 UTC 2014

Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice.

Molecular neurodegeneration

Qiu L,Qiao T,Beers M,Tan W,Wang H,Yang B,Xu Z

Thu Jan 03 00:00:00 UTC 2013

Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice.

Molecular neurodegeneration

Qiu L,Qiao T,Beers M,Tan W,Wang H,Yang B,Xu Z

Thu Jan 03 00:00:00 UTC 2013

Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice.

Molecular neurodegeneration

Qiu L,Qiao T,Beers M,Tan W,Wang H,Yang B,Xu Z

Thu Jan 03 00:00:00 UTC 2013

Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice.

Molecular neurodegeneration

Qiu L,Qiao T,Beers M,Tan W,Wang H,Yang B,Xu Z

Thu Jan 03 00:00:00 UTC 2013

Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.

Human molecular genetics

McCray BA,Skordalakes E,Taylor JP

Mon Mar 15 00:00:00 UTC 2010

Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.

Human molecular genetics

McCray BA,Skordalakes E,Taylor JP

Mon Mar 15 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: ALS 8; ALS8VAMP-B/VAMP-C; vamp; VAMP (vesicle-associated membrane protein)-associated protein B and C; Vamp 33b; VAMP B; VAMP B VAMP C; VAMP B/VAMP C; VAMP C; VAMP-associated 33 kDa protein; VAMP-associated protein 33b; VAMP-associated protein B; VAMP-B; VAMP-B/VAMP-C; VAMP-C; VAMPB; VAMPB/VAMPC; VAMPC; VAP 33b; VAP B; VAP B/VAP C; VAP C; VAP-B; VAP-B/VAP-C; VAP-C; VAPB/VAPC; VAPC; vesicle-associated membrane protein, associated protein B and C; Vesicle-associated membrane protein-associated protein B; Vesicle-associated membrane protein-associated protein B/C

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Gene Aliases: AI225786; AI840687; AI848259; ALS8; D2Abb2e; R75548; UNQ484/PRO983; VAMP-B; Vamp33b; VAP-B; VAP33b; VAPB

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UniProt ID: (Human) O95292, (Rat) Q9Z269

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Entrez Gene ID: (Human) 9217, (Rat) 60431, (Mouse) 56491

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Function(s)

protein binding microtubule binding enzyme binding FFAT motif binding protein homodimerization activity protein heterodimerization activity beta-tubulin binding cadherin binding involved in cell-cell adhesion molecular_function

Process(es)

cellular calcium ion homeostasis ER to Golgi vesicle-mediated transport activation of signaling protein activity involved in unfolded protein response endoplasmic reticulum organization biological_process modulation by virus of host morphology or physiology endoplasmic reticulum unfolded protein response negative regulation by host of viral release from host cell positive regulation by host of viral release from host cell negative regulation by host of viral genome replication positive regulation by host of viral genome replication positive regulation of viral genome replication negative regulation by virus of viral protein levels in host cell COPII-coated vesicle budding sphingolipid biosynthetic process cell-cell adhesion response to unfolded protein

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VAPB Polyclonal Antibody

VAPB Antibody (14477-1-AP) in ICC/IF

Product Details

14477-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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