Invitrogen

VCAM-1 Monoclonal Antibody (1.4C3)

VCAM-1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

VCAM-1 Antibody (MA5-11447) in ICC/IF

Immunofluorescent analysis of CD106 (green) showing staining in the cytoplasm of HUVEC cells treated with human TNF-alpha (right) compared to untreated HUVEC cells (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD106 monoclonal antibody (Product # MA5-11447) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight... View More

VCAM-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VCAM-1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

VCAM-1 Antibody in Immunohistochemistry (IHC)

VCAM-1 Antibody in Flow Cytometry (Flow)

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Product Details

MA5-11447

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:12.5-1:25

Immunohistochemistry (Frozen) (IHC (F))

1:12.5-1:25

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1.4C3

Immunogen

Stimulated human umbilical vein endothelial cells (HUVEC)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10979792

Product Specific Information

MA5-11447 targets CD106 in IHC (P, F) and ICC/IF applications and shows reactivity with Human samples.

The MA5-11447 immunogen is stimulated human umbilical vein endothelial cells (HUVEC).

MA5-11447 has been successfully used in immunohistochemistry analysis of VCAM1 in mouse E16.5 frozen lung sections.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An integrated organoid omics map extends modeling potential of kidney disease.

Nature communications

Lassé M,El Saghir J,Berthier CC,Eddy S,Fischer M,Laufer SD,Kylies D,Hutzfeldt A,Bonin LL,Dumoulin B,Menon R,Vega-Warner V,Eichinger F,Alakwaa F,Fermin D,Billing AM,Minakawa A,McCown PJ,Rose MP,Godfrey B,Meister E,Wiech T,Noriega M,Chrysopoulou M,Brandts P,Ju W,Reinhard L,Hoxha E,Grahammer F,Lindenmeyer MT,Huber TB,Schlüter H,Thiel S,Mariani LH,Puelles VG,Braun F,Kretzler M,Demir F,Harder JL,Rinschen MM

MA5-11447 was used in Immunohistochemistry to define the organoid proteome and transcriptome trajectories over culture duration and upon exposure to TNFα, a cytokine stressor.

Mon Aug 14 00:00:00 UTC 2023

An atlas of healthy and injured cell states and niches in the human kidney.

Nature

Lake BB,Menon R,Winfree S,Hu Q,Melo Ferreira R,Kalhor K,Barwinska D,Otto EA,Ferkowicz M,Diep D,Plongthongkum N,Knoten A,Urata S,Mariani LH,Naik AS,Eddy S,Zhang B,Wu Y,Salamon D,Williams JC,Wang X,Balderrama KS,Hoover PJ,Murray E,Marshall JL,Noel T,Vijayan A,Hartman A,Chen F,Waikar SS,Rosas SE,Wilson FP,Palevsky PM,Kiryluk K,Sedor JR,Toto RD,Parikh CR,Kim EH,Satija R,Greka A,Macosko EZ,Kharchenko PV,Gaut JP,Hodgin JB,Eadon MT,Dagher PC,El-Achkar TM,Zhang K,Kretzler M,Jain S

MA5-11447 was used in Immunohistochemistry to define 28 cellular states across nephron segments and interstitium that were altered in kidney injury, encompassing cycling, adaptive (successful or maladaptive repair), transitioning and degenerative states.

Sat Jul 01 00:00:00 UTC 2023

Mechanical injury accentuates lipid deposition in ApoE^-/- mice and advance aortic valve stenosis: A novel modified aortic valve stenosis model.

Frontiers in cardiovascular medicine

Wen D,Hu L,Shan J,Zhang H,Hu L,Yuan A,Pu J,Xue S

MA5-11447 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) to show thickening and fibrosis of aortic valve leaflets in the hyperlipidemia group after wire injury.

Fri Feb 24 00:00:00 UTC 2023

VCAM-1 Targeted Lipopolyplexes as Vehicles for Efficient Delivery of shRNA-Runx2 to Osteoblast-Differentiated Valvular Interstitial Cells; Implications in Calcific Valve Disease Treatment.

International journal of molecular sciences

Voicu G,Rebleanu D,Mocanu CA,Tanko G,Droc I,Uritu CM,Pinteala M,Manduteanu I,Simionescu M,Calin M

Published figure using VCAM-1 monoclonal antibody (Product # MA5-11447) in Flow Cytometry

Wed Mar 30 00:00:00 UTC 2022

Pharmacological Inhibition of Lysine-Specific Demethylase 1A Reduces Atherosclerotic Lesion Formation in Apolipoprotein E-Deficient Mice by a Mechanism Involving Decreased Oxidative Stress and Inflammation; Potential Implications in Human Atherosclerosis.

Antioxidants (Basel, Switzerland)

Manea SA,Vlad ML,Lazar AG,Muresian H,Simionescu M,Manea A

Published figure using VCAM-1 monoclonal antibody (Product # MA5-11447) in Western Blot

Thu Dec 01 00:00:00 UTC 2022

Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repair.

Science translational medicine

Gupta N,Matsumoto T,Hiratsuka K,Garcia Saiz E,Galichon P,Miyoshi T,Susa K,Tatsumoto N,Yamashita M,Morizane R

MA5-11447 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) to identify pathologic pathways and potential therapies.

Wed Mar 02 00:00:00 UTC 2022

Thrombin Inhibition Prevents Endothelial Dysfunction and Reverses 20-HETE Overproduction without Affecting Blood Pressure in Angiotensin II-Induced Hypertension in Mice.

International journal of molecular sciences

Kij A,Bar A,Przyborowski K,Proniewski B,Mateuszuk L,Jasztal A,Kieronska-Rudek A,Marczyk B,Matyjaszczyk-Gwarda K,Tworzydlo A,Enggaard C,Hansen PBL,Jensen B,Walczak M,Chlopicki S

MA5-11447 was used in Immunohistochemistry to assess the effects of the inhibition of thrombin activity by dabigatran on Ang II-induced hypertension and endothelial dysfunction in mice with a particular focus on NO- and 20-HETE-dependent pathways.

Thu Aug 12 00:00:00 UTC 2021

Innate Immune Dysfunction in Rosacea Promotes Photosensitivity and Vascular Adhesion Molecule Expression.

The Journal of investigative dermatology

Kulkarni NN,Takahashi T,Sanford JA,Tong Y,Gombart AF,Hinds B,Cheng JY,Gallo RL

MA5-11447 was used in Immunohistochemistry to show how the expression of LL-37 can lead to enhanced sensitivity to UVB radiation in rosacea.

Sun Mar 01 00:00:00 UTC 2020

Glucocorticoid regulates mesenchymal cell differentiation required for perinatal lung morphogenesis and function.

American journal of physiology. Lung cellular and molecular physiology

Bridges JP,Sudha P,Lipps D,Wagner A,Guo M,Du Y,Brown K,Filuta A,Kitzmiller J,Stockman C,Chen X,Weirauch MT,Jobe AH,Whitsett JA,Xu Y

MA5-11447 was used in Immunohistochemistry to conclude that regulating signals controlling AT2/AT1 progenitor cell proliferation and differentiation and identifying cells and processes by which glucocorticoid signaling regulates fetal lung maturation.

Sat Aug 01 00:00:00 UTC 2020

Acute Graft-vs.-Host Disease-Associated Endothelial Activation in vitro Is Prevented by Defibrotide.

Frontiers in immunology

Martinez-Sanchez J,Hamelmann H,Palomo M,Mir E,Moreno-Castaño AB,Torramade S,Rovira M,Escolar G,Cordes S,Kalupa M,Mertlitz S,Riesner K,Carreras E,Penack O,Diaz-Ricart M

MA5-11447 was used in Immunocytochemistry-immunoflourescence to show that defibrotide has notable positive effects on endothelial biology during acute graft-vs.-host disease.

Wed Oct 28 00:00:00 UTC 2020

Follicular Stimulating Hormone Accelerates Atherogenesis by Increasing Endothelial VCAM-1 Expression.

Theranostics

Li X,Chen W,Li P,Wei J,Cheng Y,Liu P,Yan Q,Xu X,Cui Y,Gu Z,Simoncini T,Fu X

Published figure using VCAM-1 monoclonal antibody (Product # MA5-11447) in Immunofluorescence

Mon Jul 09 00:00:00 UTC 2018

Inhibition of cell surface expression of endothelial adhesion molecules by ursolic acid prevents intimal hyperplasia of venous bypass grafts in rats.

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery

Zeller I,Wiedemann D,Schwaiger S,Stelzmüller M,Kreutmayer S,Leberfing O,Stuppner H,Bernhard D

MA5-11447 was used in immunohistochemistry to study the role of reduced endothelial VCAM-1 expresson in the mechanism by which ursolic acid prevents intimal hyperplasia in a rat model of venous bypass graft

Thu Nov 01 00:00:00 UTC 2012

Identification and pharmacological characterization of the anti-inflammatory principal of the leaves of dwarf elder (Sambucus ebulus L.).

Journal of ethnopharmacology

Schwaiger S,Zeller I,Pölzelbauer P,Frotschnig S,Laufer G,Messner B,Pieri V,Stuppner H,Bernhard D

MA5-11447 was used in immunocytochemistry to identify and study the active anti-inflammatory component of dwarf elder leaves

Thu Jan 27 00:00:00 UTC 2011

Leoligin, the major lignan from Edelweiss, inhibits intimal hyperplasia of venous bypass grafts.

Cardiovascular research

Reisinger U,Schwaiger S,Zeller I,Messner B,Stigler R,Wiedemann D,Mayr T,Seger C,Schachner T,Dirsch VM,Vollmar AM,Bonatti JO,Stuppner H,Laufer G,Bernhard D

MA5-11447 was used in flow cytometry to study the mechanism by which leoligin inhibits intimal hyperplasia of venous bypass grafts

Mon Jun 01 00:00:00 UTC 2009

Immunophenotypic profile and role of adhesion molecules in splenic marginal zone lymphoma with bone marrow involvement.

Leukemia & lymphoma

Florena AM,Tripodo C,Porcasi R,Ingrao S,Fadda MR,De Cantis S,Iannitto E,Franco V

MA5-11447 was used in immunohistochemistry to study the distribution and function of adhesion molecules in splenic marginal zone lymphoma

Sun Jan 01 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1

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Gene Aliases: CD106; INCAM-100; Vcam-1; VCAM1

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UniProt ID: (Human) P19320

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Entrez Gene ID: (Human) 7412, (Mouse) 22329

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Function(s)

integrin binding primary amine oxidase activity cell adhesion molecule binding

Process(es)

response to hypoxia acute inflammatory response chronic inflammatory response cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion aging response to nutrient amine metabolic process response to zinc ion response to ionizing radiation viral process membrane to membrane docking B cell differentiation extracellular matrix organization response to lipopolysaccharide response to nicotine calcium-mediated signaling using intracellular calcium source cellular response to vascular endothelial growth factor stimulus positive regulation of T cell proliferation response to ethanol regulation of immune response leukocyte tethering or rolling oxidation-reduction process cell chemotaxis interferon-gamma-mediated signaling pathway cellular response to tumor necrosis factor heart development single organismal cell-cell adhesion embryonic placenta morphogenesis chorio-allantoic fusion visceral serous pericardium development cellular response to glucose stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VCAM-1 Monoclonal Antibody (1.4C3)

VCAM-1 Antibody (MA5-11447) in ICC/IF

Product Details

MA5-11447

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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