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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • VDR Antibodies

          Invitrogen

          VDR Monoclonal Antibody (9A7)

          Advanced Verification
          47 Published Figures
          104 References
          View all (22) VDR antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite VDR Monoclonal Antibody (9A7)

          • Antibody Testing Data (2)
          • Published Figures (47)
          • Advanced Verification (3)
          VDR Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          VDR Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 52

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          VDR Antibody (MA1-710) in ICC/IF

          Immunofluorescent analysis of Vitamin D Receptor (green) showing staining in the nucleus of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Vitamin D Receptor monoclonal antibody (Product # MA1-710) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated w... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          VDR Antibody in Immunocytochemistry (ICC/IF)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Competition Assay (CA)
          VDR Antibody in Immunocytochemistry (ICC/IF)
          VDR Antibody in Immunocytochemistry (ICC/IF)
          VDR Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          VDR Antibody in Immunocytochemistry (ICC/IF)
          VDR Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          VDR Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          VDR Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          VDR Antibody in Immunohistochemistry (IHC)
          VDR Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          VDR Antibody in Immunohistochemistry (IHC)
          VDR Antibody in Immunohistochemistry (IHC)
          VDR Antibody in Immunohistochemistry (IHC)
          VDR Antibody in Immunohistochemistry (IHC)
          VDR Antibody in Immunohistochemistry (IHC)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot, Immunoprecipitation (WB, IP)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot, Immunoprecipitation (WB, IP)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Western Blot (WB)
          VDR Antibody in Immunoprecipitation (IP)
          VDR Antibody in ChIP Assay (ChIP)
          VDR Antibody in ChIP Assay (ChIP)
          VDR Antibody in ChIP Assay (ChIP)
          VDR Antibody in ChIP Assay (ChIP)
          VDR Antibody
          VDR Antibody
          VDR Antibody

          Product Details

          MA1-710

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 46 publications 46 publications

          Immunohistochemistry (IHC)

          -
          View 28 publications 28 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 5 publications 5 publications

          Immunocytochemistry (ICC/IF)

          1:10-1:100
          View 9 publications 9 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          Assay-dependent
          View 3 publications 3 publications

          ChIP assay (ChIP)

          Assay-dependent
          View 3 publications 3 publications

          Gel Shift (GS)

          Assay-dependent
          View 8 publications 8 publications

          Competition Assay (CA)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Chicken, Human, Many, Mouse, Rat

          Published species

          Avian, Chicken, Dog, Hamster, Human, Mouse, Non-human primate, Rat

          Host/Isotype

          Rat / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          9A7

          Immunogen

          Partially purified chicken intestinal cytoplasmic 1,25-(OH)2D3 receptor protein.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_326366

          Product Specific Information

          MA1-710 detects vitamin D receptor (VDR) from human, rat, mouse, hamster, chicken, amphibian, and fish tissues. This antibody does not cross-react with estrogen or glucocorticoid receptors. MA1-710 detects both the occupied and unoccupied forms of the receptor.

          MA1-710 has been successfully used in Western blot, IF, ICC, IP, ChIP, and gel shift procedures. By Western blot, this antibody detects a 58 kDa and 60 kDa protein representing VDR in avians while the VDRs of mammalian species appear as single bands within the 52-55 kDa range.

          The MA1-710 immunogen is partially purified chicken intestinal cytoplasmic 1,25-(OH)2D3 receptor protein. This antibody recognizes an epitope between amino acid residues 89 and 105 of human VDR. This region is just C-terminal to the DNA binding domain of the VDR.

          Target Information

          Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors. Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation. Studies have found VDR in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system. Repression of T-cell proliferation and interleukin-2 (IL-2) gene expression, for example, occurs when VDR binds within the IL-2 enhancer. Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)2D3 has been shown to interfere with assembly of nuclear factor of activated T-cells (NFATp)/Fos/Jun/DNA complex and subsequent IL-2 gene transcription. The corresponding gene for the vitamin D receptor is NR1I1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 1,25-dihydroxyvitamin D3 receptor; HGNC:12679; Nuclear receptor subfamily 1 group I member 1; protein phosphatase 1, regulatory subunit 163; VDR; vitamin D (1,25-dihydroxyvitamin D3) receptor; vitamin D nuclear receptor variant 1; vitamin D receptor; Vitamin D3 receptor

          View more View less

          Gene Aliases: NR1I1; PPP1R163; VDR

          View more View less

          UniProt ID: (Human) P11473, (Chicken) O42392, (Rat) P13053, (Mouse) P48281

          View more View less

          Entrez Gene ID: (Human) 7421, (Chicken) 395988, (Rat) 24873, (Mouse) 22337

          View more View less

          Function(s)
          DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity protein binding zinc ion binding calcitriol receptor activity lithocholic acid receptor activity sequence-specific DNA binding retinoid X receptor binding vitamin D response element binding calcitriol binding lithocholic acid binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding vitamin D binding metal ion binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter cell morphogenesis skeletal system development transcription initiation from RNA polymerase II promoter calcium ion transport cellular calcium ion homeostasis signal transduction lactation negative regulation of cell proliferation positive regulation of gene expression negative regulation of keratinocyte proliferation positive regulation of vitamin D 24-hydroxylase activity bile acid signaling pathway steroid hormone mediated signaling pathway positive regulation of keratinocyte differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter decidualization intestinal absorption positive regulation of apoptotic process involved in mammary gland involution regulation of calcidiol 1-monooxygenase activity mammary gland branching involved in pregnancy vitamin D receptor signaling pathway transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development heart development aging organ morphogenesis intracellular receptor signaling pathway response to estradiol response to calcium ion regulation of calcium ion transport cellular response to vitamin D apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.
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