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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • VEGF-165 Antibodies
          The PeproTech website has now moved to Thermofisher.com. All orders for PeproTech products must be placed on thermofisher.com or offline via phone, fax, email, or distributor partner. For any questions, please contact PeproTech.CustomerService@thermofisher.com

          Invitrogen

          VEGF-165 Polyclonal Antibody, PeproTech®

          1 Published Figure
          6 References
          View all (13) VEGF-165 antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite VEGF-165 Polyclonal Antibody, PeproTech®

          • Antibody Testing Data (3)
          • Published Figures (1)
          VEGF-165 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          VEGF-165 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          VEGF-165 Antibody (500-P131-1MG) in WB

          Western Blot: To detect Murine VEGF 165 by Western Blot analysis VEGF-165 Polyclonal Antibody (Product # 500-P131-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine VEGF165 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          VEGF-165 Antibody in Western Blot (WB)
          VEGF-165 Antibody in Western Blot (WB)
          VEGF-165 Antibody in ELISA (ELISA)
          VEGF-165 Antibody in Western Blot (WB)

          Product Details

          500-P131-1MG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.2 µg/mL
          View 3 publications 3 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          0.5-2.0 µg/mL
          View 1 publication 1 publication

          Neutralization (Neu)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Mouse, Rat

          Host/Isotype

          Rabbit

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived Recombinant Murine VEGF 165

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          0.1-1.0 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient

          RRID

          AB_2929389

          Product Specific Information

          AA Sequence of recombinant protein: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

          Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine VEGF 165. Anti-Murine VEGF 165-specific antibody was purified by affinity chromatography employing an immobilized Murine VEGF 165 matrix.

          Sandwich ELISA: To detect Murine VEGF 165 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine VEGF 165 (500-P131Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine VEGF 165.

          Western Blot: To detect Murine VEGF 165 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine VEGF165 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

          Target Information

          The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: H-VEGF-165; L-VEGF; M-VEGF-165; vascular endothelial growth factor 165b; vascular endothelial growth factor A; Vascular endothelial growth factor A, long form; Vascular permeability factor; VEGF165; VPF

          View more View less

          Gene Aliases: Vegf; Vegfa; Vpf

          View more View less

          UniProt ID: (Mouse) Q00731

          View more View less

          Entrez Gene ID: (Mouse) 22339

          View more View less

          Function(s)
          fibronectin binding receptor binding cytokine activity platelet-derived growth factor receptor binding vascular endothelial growth factor receptor binding protein binding growth factor activity heparin binding neuropilin binding chemoattractant activity identical protein binding protein homodimerization activity vascular endothelial growth factor receptor 1 binding vascular endothelial growth factor receptor 2 binding protein heterodimerization activity receptor agonist activity extracellular matrix binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter angiogenesis ovarian follicle development blood vessel development patterning of blood vessels response to hypoxia in utero embryonic development kidney development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation vasculature development lymphangiogenesis blood vessel remodeling sprouting angiogenesis cell migration involved in sprouting angiogenesis positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation positive regulation of receptor internalization basophil chemotaxis heart morphogenesis outflow tract morphogenesis coronary vein morphogenesis regulation of transcription from RNA polymerase II promoter multicellular organism development nervous system development mesoderm development lactation cell proliferation positive regulation of cell proliferation regulation of cell shape positive regulation of signal transduction positive regulation of endothelial cell migration positive regulation of gene expression cell migration negative regulation of cell-cell adhesion cell differentiation monocyte differentiation macrophage differentiation lung development positive regulation of cell migration regulation of cGMP metabolic process epithelial cell differentiation positive regulation of vascular endothelial growth factor receptor signaling pathway post-embryonic camera-type eye development positive regulation of protein complex assembly positive regulation of protein autophosphorylation activation of protein kinase activity positive regulation of CREB transcription factor activity positive regulation of peptidyl-serine phosphorylation tube formation endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus lymph vessel morphogenesis positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway vascular endothelial growth factor signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway VEGF-activated neuropilin signaling pathway growth T-helper 1 type immune response eye photoreceptor cell development negative regulation of apoptotic process negative regulation of programmed cell death positive regulation of vascular permeability surfactant homeostasis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity negative regulation of neuron apoptotic process positive regulation of blood vessel endothelial cell migration endothelial cell migration regulation of endothelial cell differentiation positive regulation of angiogenesis negative regulation of bone resorption positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway mRNA stabilization lung alveolus development cell maturation blood vessel morphogenesis camera-type eye morphogenesis positive regulation of smooth muscle cell proliferation neuron development cardiac muscle fiber development branching morphogenesis of an epithelial tube positive regulation of axon extension involved in axon guidance artery morphogenesis positive regulation of epithelial cell proliferation positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of positive chemotaxis induction of positive chemotaxis positive regulation of cellular component movement positive regulation of cell division positive regulation of focal adhesion assembly positive regulation of protein kinase B signaling primitive erythrocyte differentiation cell chemotaxis lung vasculature development mammary gland alveolus development positive regulation of mast cell chemotaxis cardiac vascular smooth muscle cell development angiogenesis involved in coronary vascular morphogenesis coronary artery morphogenesis positive regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of ERK1 and ERK2 cascade cellular response to hypoxia dopaminergic neuron differentiation commissural neuron axon guidance positive regulation of protein kinase C signaling positive regulation of cell migration involved in sprouting angiogenesis positive regulation of branching involved in ureteric bud morphogenesis regulation of retinal ganglion cell axon guidance positive regulation of peptidyl-tyrosine autophosphorylation positive regulation of p38MAPK cascade negative regulation of neuron death positive regulation of lymphangiogenesis regulation of hematopoietic progenitor cell differentiation positive regulation of histone deacetylase activity positive regulation of retinal ganglion cell axon guidance positive regulation of protein localization to early endosome positive regulation of protein kinase D signaling positive regulation of receptor activity negative regulation of extrinsic apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.
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