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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • VEGF Antibodies

          Invitrogen

          VEGF Monoclonal Antibody (VG1)

          16 Published Figures
          77 References
          View all (88) VEGF antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite VEGF Monoclonal Antibody (VG1)

          • Antibody Testing Data (6)
          • Published Figures (16)
          VEGF Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          VEGF Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 22

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          VEGF Antibody (MA1-16629) in ICC/IF

          Immunofluorescence analysis of VEGF was performed using DU 145 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with VEGF Monoclonal Antibody (VG1) (Product # MA1-16629) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 diluti... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          VEGF Antibody in Immunocytochemistry (ICC/IF)
          VEGF Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Flow Cytometry (Flow)
          VEGF Antibody in Flow Cytometry (Flow)
          VEGF Antibody in Immunohistochemistry (IHC)
          VEGF Antibody in Immunohistochemistry (IHC)
          VEGF Antibody in Immunohistochemistry (IHC)
          VEGF Antibody in Immunohistochemistry (IHC)
          VEGF Antibody in Immunohistochemistry (IHC)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)

          Product Details

          MA1-16629

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          View 17 publications 17 publications

          Immunohistochemistry (IHC)

          -
          View 52 publications 52 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:100
          View 5 publications 5 publications

          Immunohistochemistry (Frozen) (IHC (F))

          1:20-1:100
          -

          Immunocytochemistry (ICC/IF)

          1:10-1:500
          View 3 publications 3 publications

          Flow Cytometry (Flow)

          Assay-Dependent
          -

          ELISA (ELISA)

          Assay-Dependent
          -
          Product Specifications

          Species Reactivity

          Dog, Human, Mouse, Pig, Rat

          Published species

          Camelid, Human, Mouse, Rabbit, Rat

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          VG1

          Immunogen

          Recombinant VEGF protein.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2212682

          Product Specific Information

          For IHC, incubate for a period of 30-60 minutes at room temperature.

          High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to the immunostaining.

          Target Information

          VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. Studies have shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. Elevated levels of VEGF is linked to POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes) also known as Crow-Fukase syndrome which affects multiple organs in the body.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: L-VEGF; MGC70609; RP1-261G23.1; VAS; vascular endothelial growth factor 165b; vascular endothelial growth factor 188; Vascular endothelial growth factor A; Vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; Vasculotropin; VEGF-A; VEGF1; VEGFMGC70609; VPF; VPF ant

          View more View less

          Gene Aliases: MVCD1; VEGF; VEGF-A; VEGF164; VEGFA; VPF

          View more View less

          UniProt ID: (Human) P15692, (Dog) Q9MYV3, (Pig) P49151, (Rat) P16612, (Mouse) Q00731

          View more View less

          Entrez Gene ID: (Human) 7422, (Dog) 403802, (Pig) 397157, (Rat) 83785, (Mouse) 22339

          View more View less

          Function(s)
          fibronectin binding cytokine activity platelet-derived growth factor receptor binding vascular endothelial growth factor receptor binding protein binding growth factor activity heparin binding chemoattractant activity identical protein binding protein homodimerization activity vascular endothelial growth factor receptor 1 binding vascular endothelial growth factor receptor 2 binding protein heterodimerization activity receptor agonist activity extracellular matrix binding receptor binding neuropilin binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter angiogenesis ovarian follicle development patterning of blood vessels vasculogenesis response to hypoxia in utero embryonic development kidney development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation positive regulation of receptor internalization platelet degranulation positive regulation of leukocyte migration heart morphogenesis outflow tract morphogenesis coronary vein morphogenesis nervous system development mesoderm development lactation positive regulation of cell proliferation regulation of cell shape positive regulation of endothelial cell migration positive regulation of gene expression monocyte differentiation macrophage differentiation lung development positive regulation of cell migration regulation of cGMP metabolic process epithelial cell differentiation positive regulation of vascular endothelial growth factor receptor signaling pathway post-embryonic camera-type eye development positive regulation of protein complex assembly positive regulation of protein autophosphorylation activation of protein kinase activity positive regulation of CREB transcription factor activity positive regulation of peptidyl-serine phosphorylation tube formation endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus lymph vessel morphogenesis positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway VEGF-activated neuropilin signaling pathway growth eye photoreceptor cell development positive regulation of tyrosine phosphorylation of Stat3 protein negative regulation of apoptotic process surfactant homeostasis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity positive regulation of blood vessel endothelial cell migration positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway cell maturation camera-type eye morphogenesis cardiac muscle fiber development branching morphogenesis of an epithelial tube positive regulation of axon extension involved in axon guidance artery morphogenesis positive regulation of epithelial cell proliferation positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of positive chemotaxis induction of positive chemotaxis positive regulation of cellular component movement positive regulation of cell division positive regulation of focal adhesion assembly primitive erythrocyte differentiation mammary gland alveolus development cardiac vascular smooth muscle cell development coronary artery morphogenesis regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of transcription from RNA polymerase II promoter in response to hypoxia cellular response to hypoxia dopaminergic neuron differentiation commissural neuron axon guidance positive regulation of protein kinase C signaling positive regulation of cell migration involved in sprouting angiogenesis positive regulation of branching involved in ureteric bud morphogenesis regulation of retinal ganglion cell axon guidance positive regulation of peptidyl-tyrosine autophosphorylation positive regulation of p38MAPK cascade positive regulation of histone deacetylase activity positive regulation of retinal ganglion cell axon guidance positive regulation of protein kinase D signaling blood vessel development vasculature development lymphangiogenesis blood vessel remodeling sprouting angiogenesis basophil chemotaxis regulation of transcription from RNA polymerase II promoter female pregnancy aging cell proliferation response to cold positive regulation of signal transduction cell migration negative regulation of cell-cell adhesion cell differentiation hyaluronan metabolic process response to estradiol response to progesterone response to vitamin A cellular response to drug vascular endothelial growth factor signaling pathway wound healing T-helper 1 type immune response negative regulation of programmed cell death positive regulation of vascular permeability negative regulation of neuron apoptotic process endothelial cell migration cellular response to fibroblast growth factor stimulus regulation of endothelial cell differentiation negative regulation of bone resorption mRNA stabilization lung alveolus development blood vessel morphogenesis positive regulation of smooth muscle cell proliferation neuron development response to folic acid positive regulation of protein kinase B signaling cell chemotaxis lung vasculature development positive regulation of mast cell chemotaxis angiogenesis involved in coronary vascular morphogenesis positive regulation of ERK1 and ERK2 cascade response to oxygen levels response to dexamethasone cellular response to dexamethasone stimulus negative regulation of neuron death positive regulation of lymphangiogenesis regulation of hematopoietic progenitor cell differentiation positive regulation of protein localization to early endosome negative regulation of extrinsic apoptotic signaling pathway multicellular organism development positive regulation of receptor activity
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