Invitrogen

VEGF Receptor 2 Monoclonal Antibody (B.309.4)

VEGF Receptor 2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

VEGF Receptor 2 Antibody (MA5-15157) in ICC/IF

Immunofluorescence analysis of VEGF Receptor 2 was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with VEGF Receptor 2 Monoclonal Antibody (B.309.4) (Product # MA5-15157) at 1:200 dilution in 0.1% BSA, inc... View More

VEGF Receptor 2 Antibody in Immunohistochemistry (IHC)

VEGF Receptor 2 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

VEGF Receptor 2 Antibody in Western Blot (WB)

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Product Details

MA5-15157

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:4000-1:16000

Immunohistochemistry (Frozen) (IHC (F))

1:200-1:800

Immunocytochemistry (ICC/IF)

1:200-1:800

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

1:100

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

B.309.4

Immunogen

Recombinant protein containing the carboxy-terminal 150 amino acid residues of human VEGF receptor 2 (aa 1206-1356).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

28 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10986085

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other family members.

Target Information

KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PD-L1-directed PlGF/VEGF blockade synergizes with chemotherapy by targeting CD141⁺ cancer-associated fibroblasts in pancreatic cancer.

Nature communications

Kim DK,Jeong J,Lee DS,Hyeon DY,Park GW,Jeon S,Lee KB,Jang JY,Hwang D,Kim HM,Jung K

MA5-15157 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to study the mechanism underlying chemotherapy-induced fibrosis in PDAC.

Sat Oct 22 00:00:00 UTC 2022

PD-L1-directed PlGF/VEGF blockade synergizes with chemotherapy by targeting CD141⁺ cancer-associated fibroblasts in pancreatic cancer.

Nature communications

Kim DK,Jeong J,Lee DS,Hyeon DY,Park GW,Jeon S,Lee KB,Jang JY,Hwang D,Kim HM,Jung K

Sat Oct 22 00:00:00 UTC 2022

Cyclooxygenase inhibition attenuates brain angiogenesis and independently decreases mouse survival under hypoxia.

Journal of neurochemistry

Seeger DR,Golovko SA,Grove BD,Golovko MY

MA5-15157 was used in Western Blotting to indicate a potential risk for using COX inhibitors under hypoxia conditions in clinics.

Thu Jul 01 00:00:00 UTC 2021

Cyclooxygenase inhibition attenuates brain angiogenesis and independently decreases mouse survival under hypoxia.

Journal of neurochemistry

Seeger DR,Golovko SA,Grove BD,Golovko MY

MA5-15157 was used in Western Blotting to indicate a potential risk for using COX inhibitors under hypoxia conditions in clinics.

Thu Jul 01 00:00:00 UTC 2021

Functional damage of endothelial progenitor cells is attenuated by 14-3-3-n through inhibition of mitochondrial injury and oxidative stress.

Cell biology international

Li Y,Sun X,Zhang X,Zhou H,Wang D,Xia Y,Li X

MA5-15157 was used in Cell Culture to provide a new target, 14-3-3-η, for the prevention and treatment of cardiovascular diseases through targeting EPCs.

Thu Apr 01 00:00:00 UTC 2021

Functional damage of endothelial progenitor cells is attenuated by 14-3-3-n through inhibition of mitochondrial injury and oxidative stress.

Cell biology international

Li Y,Sun X,Zhang X,Zhou H,Wang D,Xia Y,Li X

MA5-15157 was used in Cell Culture to provide a new target, 14-3-3-η, for the prevention and treatment of cardiovascular diseases through targeting EPCs.

Thu Apr 01 00:00:00 UTC 2021

Functional damage of endothelial progenitor cells is attenuated by 14-3-3-n through inhibition of mitochondrial injury and oxidative stress.

Cell biology international

Li Y,Sun X,Zhang X,Zhou H,Wang D,Xia Y,Li X

MA5-15157 was used in Cell Culture to provide a new target, 14-3-3-η, for the prevention and treatment of cardiovascular diseases through targeting EPCs.

Thu Apr 01 00:00:00 UTC 2021

Targeting QKI-7 in vivo restores endothelial cell function in diabetes.

Nature communications

Yang C,Eleftheriadou M,Kelaini S,Morrison T,González MV,Caines R,Edwards N,Yacoub A,Edgar K,Moez A,Ivetic A,Zampetaki A,Zeng L,Wilkinson FL,Lois N,Stitt AW,Grieve DJ,Margariti A

MA5-15157 was used in Western Blotting to show that Quaking-7 (QKI-7) is upregulated in iPS cell-derived endothelial cells(ECs) when exposed to hyperglycemia, and in human iPS-ECs from diabetic patients.

Thu Jul 30 00:00:00 UTC 2020

VEGF Upregulates EGFR Expression to Stimulate Chemotactic Behaviors in the rMC-1 Model of Müller Glia.

Brain sciences

Peña JS,Vazquez M

MA5-15157 was used in Immunocytochemistry to show an interplay between VEGF and EGF-R pathways in Müller glia.

Fri May 29 00:00:00 UTC 2020

Blockade of insulin receptor substrate-1 inhibits biological behavior of choroidal endothelial cells.

International journal of ophthalmology

Qian YY,Wu HY,Liu GQ,Ren C,Lu PR,Zhang XG

MA5-15157 was used in Western Blotting to investigate the effects of blockade of insulin receptor substrate-1 (IRS-1) on the bio-function of tube formation of human choroidal endothelial cells (HCECs).

Wed Sep 30 00:00:00 UTC 2020

Inhibition of Endothelial SCUBE2 (Signal Peptide-CUB-EGF Domain-Containing Protein 2), a Novel VEGFR2 (Vascular Endothelial Growth Factor Receptor 2) Coreceptor, Suppresses Tumor Angiogenesis.

Arteriosclerosis, thrombosis, and vascular biology

Lin YC,Liu CY,Kannagi R,Yang RB

Published figure using VEGF Receptor 2 monoclonal antibody (Product # MA5-15157) in Western Blot

Tue May 01 00:00:00 UTC 2018

Generation of Hepatic Stellate Cells from Human Pluripotent Stem Cells Enables In Vitro Modeling of Liver Fibrosis.

Cell stem cell

Coll M,Perea L,Boon R,Leite SB,Vallverdú J,Mannaerts I,Smout A,El Taghdouini A,Blaya D,Rodrigo-Torres D,Graupera I,Aguilar-Bravo B,Chesne C,Najimi M,Sokal E,Lozano JJ,van Grunsven LA,Verfaillie CM,Sancho-Bru P

MA5-15157 was used in Western Blotting to report efficient differentiation of human pluripotent stem cells into HSC-like cells (iPSC-HSCs).

Thu Jul 05 00:00:00 UTC 2018

Brain microvasculature defects and Glut1 deficiency syndrome averted by early repletion of the glucose transporter-1 protein.

Nature communications

Tang M,Gao G,Rueda CB,Yu H,Thibodeaux DN,Awano T,Engelstad KM,Sanchez-Quintero MJ,Yang H,Li F,Li H,Su Q,Shetler KE,Jones L,Seo R,McConathy J,Hillman EM,Noebels JL,De Vivo DC,Monani UR

MA515157 was used in western blot to test if glucose transporter-1 protein arrests cerebral angiogenesis in vivo

Fri Jan 20 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: CD309; CD309 antigen; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; KDR; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; soluble VEGFR2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2

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Gene Aliases: 6130401C07; CD309; Flk-1; FLK1; KDR; Krd-1; Ly73; sVEGFR-2; VEGFR; VEGFR-2; VEGFR2

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UniProt ID: (Human) P35968, (Mouse) P35918

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Entrez Gene ID: (Human) 3791, (Mouse) 16542

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Function(s)

protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity vascular endothelial growth factor-activated receptor activity integrin binding protein binding ATP binding growth factor binding Hsp90 protein binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups vascular endothelial growth factor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VEGF Receptor 2 Monoclonal Antibody (B.309.4)

VEGF Receptor 2 Antibody (MA5-15157) in ICC/IF

Product Details

MA5-15157

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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