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Invitrogen
VEGFB Polyclonal Antibody
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VEGFB Antibody (PA5-80220) in IHC (P)
Immunohistochemistry analysis of VEGFB on paraffin-embedded rat skeletal muscle tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with VEGFB polyclonal antibody (Product# PA5-80220) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). ... View More
Product Details
PA5-80220
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived rat VEGFB recombinant protein (Position: P22-A207).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747334
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat heart tissue. IHC: mouse heart tissue, mouse spleen tissue, rat skeletal muscle tissue, rat spleen tissue.
Target Information
Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF. There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186. VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted. VEGFB stimulates endothelial cell proliferation. VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2. Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas. It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis. The genes that encode VEGFB have been mapped to chromosome 11q13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Vascular endothelial growth factor B; VEGF-B; VEGF-related factor; VRF
Gene Aliases: VEGF-B; Vegfb; Vrf
UniProt ID: (Rat) O35485, (Mouse) P49766
Entrez Gene ID: (Rat) 89811, (Mouse) 22340
angiogenesis
positive regulation of endothelial cell proliferation
protein O-linked glycosylation
heart development
negative regulation of gene expression
positive regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of vascular wound healing
response to drug
negative regulation of apoptotic process
positive regulation of vascular permeability
negative regulation of neuron apoptotic process
positive regulation of peptidyl-tyrosine phosphorylation
positive chemotaxis
induction of positive chemotaxis
positive regulation of cell division
positive regulation of protein kinase B signaling
cardiac muscle contraction
positive regulation of mast cell chemotaxis
coronary vasculature development
positive regulation of ERK1 and ERK2 cascade
multicellular organism development
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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