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Invitrogen
VGAT Polyclonal Antibody
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VGAT Antibody (PA5-27569) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of VGAT was performed in Cultured rat E18 primary cortical neuron, DIV 8. Cells fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT Polyclonal Antibody (Product # PA5-27569) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody. Blue: Fluoroshield with DAPI.
Product Details
PA5-27569
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human VGAT. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.46 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2545045
Product Specific Information
Recommended positive controls: SLC32A1-transfected 293T.
Predicted reactivity: Mouse (97%), Rat (95%), Xenopus laevis (83%), Rhesus Monkey (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GABA and glycine transporter; GVAT; hVIAAT; mVGAT; mVIAAT; rat UNC-47 homolog; rGVAT; RUNC-47; solute carrier family 32 (GABA vesicular transporter), member 1; Solute carrier family 32 member 1; vasicular inhibitory amino acid transporter 10D; Vesicular GABA and glycine transporter; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter
Gene Aliases: R75019; SLC32A1; VGAT; VIAAT
UniProt ID: (Human) Q9H598, (Rat) O35458, (Mouse) O35633
Entrez Gene ID: (Human) 140679, (Rat) 83612, (Mouse) 22348
amino acid transmembrane transport
neurotransmitter transport
aging
gamma-aminobutyric acid transport
glycine transport
proton transport
sodium ion transmembrane transport
L-alpha-amino acid transmembrane transport
transport
ion transport
neurotransmitter secretion
amine transport
hippocampus development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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